################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I ApaI ApaLI ApoI AscI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BseAI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspMI BsrBI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO109I EcoRI EcoRV FauI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeI MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 265 GTAGAC GT^[AC][GT]AC AflIII 824 ACATGT A^C[GA][CT]GT AocI 484 CCTAAGG CC^T[ACGT]AGG Aor51HI 371 AGCGCT AGC^GCT AseI 565 ATTAAT AT^TAAT AsnI 565 ATTAAT AT^TAAT Asp700I 738 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 208 GAGCAC G[AT]GC[AT]^C BanI 248 GGCACC G^G[CT][GA]CC BclI 107 TGATCA T^GATCA BcnI 534 CCGGG CC^[GC]GG BsaBI 345 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 507 ACCGGA [AT]^CCGG[AT] BseRI 156 CTCCTC GAGGAG(10/8) BsiEI 461 CGGTCG CG[GA][CT]^CG BsiHKAI 208 GAGCAC G[AT]GC[AT]^C BsmFI 833 GGGAC GGGAC(10/14) BsmI 54 GAATGC GAATGC(1/-1) Bsp1407I 842 TGTACA T^GTACA BspLU11I 824 ACATGT A^CATGT BsrDI 786 CATTGC GCAATG(2/0) BsrFI 1220 GCCGGC [GA]^CCGG[CT] BsrGI 842 TGTACA T^GTACA BssSI 1020 CACGAG CACGAG(-5/-1) BstEII 20 GGTCACC G^GT[ACGT]ACC BstPI 20 GGTCACC G^GT[ACGT]ACC BstYI 350 AGATCC [GA]^GATC[CT] Bsu36I 484 CCTAAGG CC^T[ACGT]AGG Cfr10I 1220 GCCGGC [GA]^CCGG[CT] Cfr13I 545 GGGCC G^G[ACGT]CC DsaI 386 CCATGG C^C[GA][CT]GG EarI 1027 CTCTTC CTCTTC(1/4) Eco47III 371 AGCGCT AGC^GCT Eco81I 484 CCTAAGG CC^T[ACGT]AGG EcoO65I 20 GGTCACC G^GT[ACGT]ACC EcoT22I 718 ATGCAT ATGCA^T FbaI 107 TGATCA T^GATCA HaeII 371 AGCGCT [GA]GCGC^[CT] Ksp632I 1027 CTCTTC CTCTTC(1/4) MaeII 995 ACGT A^CGT MamI 345 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 350 AGATCC [GA]^GATC[CT] NaeI 1220 GCCGGC GCC^GGC NciI 534 CCGGG CC^[GC]GG NcoI 386 CCATGG C^CATGG NgoMIV 1220 GCCGGC G^CCGGC NsiI 718 ATGCAT ATGCA^T NspI 824 ACATGT [GA]CATG^[CT] PshBI 565 ATTAAT AT^TAAT Sau96I 545 GGGCC G^G[ACGT]CC ScaI 1124 AGTACT AGT^ACT SexAI 711 ACCTGGT A^CC[AT]GGT SspBI 842 TGTACA T^GTACA TaqI 1158 TCGA T^CGA TfiI 953 GAATC G^A[AT]TC Tsp45I 21 GTCAC ^GT[GC]AC TthHB8I 1158 TCGA T^CGA XcmI 356 CCATGCCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 350 AGATCC [GA]^GATC[CT] XmnI 738 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 174 GCGG CCGC(-3/-1) 848 GCGG AlwI 351 GATCC GGATC(4/5) 576 GATCC AlwNI 601 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 707 CAGTACCTG BmyI 208 GAGCAC G[AGT]GC[ACT]^C 247 GGGCAC BsgI 493 GTGCAG GTGCAG(16/14) 538 GTGCAG Bsp1286I 208 GAGCAC G[AGT]GC[ACT]^C 247 GGGCAC BstF5I 270 CATCC GGATG(2/0) 317 GGATG BstXI 26 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 237 CCATGCCAATGG CfoI 372 GCGC GCG^C 973 GCGC EcoT14I 386 CCATGG C^C[AT][AT]GG 1143 CCAAGG FokI 270 CATCC GGATG(9/13) 317 GGATG HhaI 372 GCGC GCG^C 973 GCGC HinP1I 372 GCGC G^CGC 973 GCGC HindIII 739 AAGCTT A^AGCTT 1073 AAGCTT MaeIII 21 GTCAC ^GT[ACGT]AC 694 GTAAC MspA1I 172 CAGCGG C[AC]G^C[GT]G 846 CAGCGG NlaIV 176 GGCTCC GG[ACGT]^[ACGT]CC 248 GGCACC SfaNI 318 GATGC GCATC(5/9) 296 GCATC StyI 386 CCATGG C^C[AT][AT]GG 1143 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AfaI AluI BalI BbvI BpmI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI Cac8I DdeI DpnII EaeI EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MwoI NdeII NlaIII PflMI PleI RsaI Sau3AI ScrFI Tru9I TseI Tsp509I TspRI Van91I