(Circular) (Six-base) MAPSORT of: pFN21AE3721.seq Check: 6629 from: 1 to: 5944 pFN21AE3721 5944 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3754 278 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2271 3287 1937 Size: 334 1016 4594 Fragments arranged by size: 4594 1016 334 Acc65I G'GTAC_C Cuts at: 3265 3265 Size: 5944 AclI AA'CG_TT Cuts at: 1563 4071 4444 1563 Size: 2508 373 3063 Fragments arranged by size: 3063 2508 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2281 4007 5391 5659 1632 Size: 649 1726 1384 268 1917 Fragments arranged by size: 1917 1726 1384 649 268 AfeI AGC'GCT Cuts at: 2378 2378 Size: 5944 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5747 AflIII A'CryG_T Cuts at: 1203 2829 4843 5820 1203 Size: 1626 2014 977 1327 Fragments arranged by size: 2014 1626 1327 977 AgeI A'CCGG_T Cuts at: 4775 4775 Size: 5944 AhdI GACnn_n'nnGTC Cuts at: 4673 4673 Size: 5944 AleI CACnn'nnGTG Cuts at: 1166 2453 1166 Size: 1287 4657 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5944 AlwNI CAG_nnn'CTG Cuts at: 2611 2717 3687 5259 2611 Size: 106 970 1572 3296 Fragments arranged by size: 3296 1572 970 106 ApaLI G'TGCA_C Cuts at: 4001 5157 4001 Size: 1156 4788 ApoI r'AATT_y Cuts at: 1493 3253 3479 3631 5740 1493 Size: 1760 226 152 2109 1697 Fragments arranged by size: 2109 1760 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2571 4498 160 Size: 872 1539 1927 1606 Fragments arranged by size: 1927 1606 1539 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 AvaI C'yCGr_G Cuts at: 1870 1943 3269 1870 Size: 73 1326 4545 Fragments arranged by size: 4545 1326 73 BamHI G'GATC_C Cuts at: 3274 3641 3274 Size: 367 5577 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2253 3265 4720 618 Size: 325 217 627 466 1012 1455 1842 Fragments arranged by size: 1842 1455 1012 627 466 325 217 BanII G_rGCy'C Cuts at: 1391 3263 1391 Size: 1872 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4973 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4135 4169 1903 Size: 34 2198 34 3678 Fragments arranged by size: 3678 2198 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3836 5046 3836 Size: 1210 4734 BclI T'GATC_A Cuts at: 1548 2112 1548 Size: 564 5380 BfrBI ATG'CAT Cuts at: 2725 2725 Size: 5944 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4555 136 Size: 107 122 71 631 605 2883 1525 Fragments arranged by size: 2883 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5939 5939 Size: 5944 BlpI GC'TnA_GC Cuts at: 3361 3361 Size: 5944 Bme1580I G_kGCm'C Cuts at: 1790 2256 4005 5161 1790 Size: 466 1749 1156 2573 Fragments arranged by size: 2573 1749 1156 466 BmgBI CAC'GTC Cuts at: 1318 3001 1318 Size: 1683 4261 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2705 2767 3675 Size: 416 53 83 399 24 1288 62 908 Cuts at: 3675 4633 442 Size: 958 1753 Fragments arranged by size: 1753 1288 958 908 416 399 83 62 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2108 2393 2817 4604 1239 Size: 836 33 285 424 1787 2579 Fragments arranged by size: 2579 1787 836 424 285 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4069 4934 5232 4069 Size: 865 298 4781 Fragments arranged by size: 4781 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4607 882 Size: 617 3108 2219 Fragments arranged by size: 3108 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3735 493 Size: 711 2531 2702 Fragments arranged by size: 2702 2531 711 BsaBI GATnn'nnATC Cuts at: 2354 5938 2354 Size: 3584 2360 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3751 4133 4768 275 Size: 53 83 186 936 2218 382 635 1451 Fragments arranged by size: 2218 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2512 4377 4775 5049 5196 5607 2512 Size: 1865 398 274 147 411 2849 Fragments arranged by size: 2849 1865 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5719 Fragments arranged by size: 5719 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2152 1186 Size: 966 4978 BseYI C'CCAG_C Cuts at: 5147 5643 5147 Size: 496 5448 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2519 2564 1163 Size: 573 783 45 4543 Fragments arranged by size: 4543 783 573 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2469 3394 4155 5183 1540 Size: 275 186 468 925 761 1028 2301 Fragments arranged by size: 2301 1028 925 761 468 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 4005 4090 5161 2217 Size: 1788 85 1071 3000 Fragments arranged by size: 3000 1788 1071 85 BsmI GAATG_Cn' Cuts at: 2065 3455 3548 2065 Size: 1390 93 4461 Fragments arranged by size: 4461 1390 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2256 3263 4005 4090 5161 1391 Size: 399 427 39 1007 742 85 1071 2174 Fragments arranged by size: 2174 1071 1007 742 427 399 85 39 BspEI T'CCGG_A Cuts at: 5607 5607 Size: 5944 BspHI T'CATG_A Cuts at: 3833 5563 3833 Size: 1730 4214 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3299 844 Size: 2455 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3831 1394 Size: 429 2008 3507 Fragments arranged by size: 3507 2008 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2790 4439 4613 65 Size: 1183 1542 1649 174 1396 Fragments arranged by size: 1649 1542 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3225 4588 4775 1700 Size: 110 1415 1363 187 2869 Fragments arranged by size: 2869 1415 1363 187 110 BsrGI T'GTAC_A Cuts at: 96 2847 96 Size: 2751 3193 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5944 BssSI C'ACGA_G Cuts at: 3025 4004 5016 3025 Size: 979 1012 3953 Fragments arranged by size: 3953 1012 979 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5944 BstBI TT'CG_AA Cuts at: 4748 4748 Size: 5944 BstEII G'GTnAC_C Cuts at: 1184 2025 1184 Size: 841 5103 BstXI CCAn_nnnn'nTGG Cuts at: 2038 2249 2038 Size: 211 5733 BstYI r'GATC_y Cuts at: 1972 2355 3274 3641 4026 4043 5495 5581 5593 Size: 383 919 367 385 17 1452 86 12 Cuts at: 5593 5939 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 919 385 383 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2490 2490 Size: 5944 BtgI C'CryG_G Cuts at: 513 1065 2391 513 Size: 552 1326 4066 Fragments arranged by size: 4066 1326 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5205 Fragments arranged by size: 5205 683 56 BtsI GCAGTG_nn' Cuts at: 2552 3460 4254 4274 2552 Size: 908 794 20 4222 Fragments arranged by size: 4222 908 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5944 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2815 2850 529 Size: 35 2251 35 3623 Fragments arranged by size: 3623 2251 35 35 DraI TTT'AAA Cuts at: 3248 3248 Size: 5944 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4951 809 Size: 507 3635 1802 Fragments arranged by size: 3635 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2239 2854 3054 Size: 54 1425 50 275 18 409 615 200 Cuts at: 3054 3099 3188 3391 4280 5909 8 Size: 45 89 203 889 1629 43 Fragments arranged by size: 1629 1425 889 615 409 275 203 200 89 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3391 1537 Size: 275 1579 4090 Fragments arranged by size: 4090 1579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3038 3874 1353 Size: 1685 836 3423 Fragments arranged by size: 3423 1685 836 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4531 4905 5051 387 Size: 1064 138 2942 374 146 1280 Fragments arranged by size: 2942 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2108 2281 2393 2817 4007 4604 Size: 393 443 33 173 112 424 1190 597 Cuts at: 4604 5391 5659 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1190 787 597 443 424 393 268 173 112 33 EcoRI G'AATT_C Cuts at: 3253 3631 3253 Size: 378 5566 FspI TGC'GCA Cuts at: 4450 4450 Size: 5944 HaeII r_GCGC'y Cuts at: 1678 2380 5091 1678 Size: 702 2711 2531 Fragments arranged by size: 2711 2531 702 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4585 4617 4659 4691 1373 Size: 32 135 32 3013 32 42 32 2626 Fragments arranged by size: 3013 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3288 3534 669 Size: 988 281 1350 246 3079 Fragments arranged by size: 3079 1350 988 281 246 HindIII A'AGCT_T Cuts at: 748 2744 3078 748 Size: 1996 334 3614 Fragments arranged by size: 3614 1996 334 HpaI GTT'AAC Cuts at: 3534 3534 Size: 5944 KpnI G_GTAC'C Cuts at: 3269 3269 Size: 5944 MfeI C'AATT_G Cuts at: 3543 3543 Size: 5944 MluI A'CGCG_T Cuts at: 5820 5820 Size: 5944 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2935 5058 5242 1305 Size: 1630 2123 184 2007 Fragments arranged by size: 2123 2007 1630 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3056 3101 3190 10 Size: 54 1425 343 1224 45 89 2764 Fragments arranged by size: 2764 1425 1224 343 89 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2247 2453 2456 2864 3902 4261 4420 Size: 648 1081 206 3 408 1038 359 159 Cuts at: 4420 518 Size: 2042 Fragments arranged by size: 2042 1081 1038 648 408 359 206 159 3 MspA1I CmG'CkG Cuts at: 1606 2179 2853 3360 4037 5185 5430 1606 Size: 573 674 507 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 674 573 507 245 NaeI GCC'GGC Cuts at: 3227 3227 Size: 5944 NcoI C'CATG_G Cuts at: 513 1065 2391 513 Size: 552 1326 4066 Fragments arranged by size: 4066 1326 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5944 NgoMIV G'CCGG_C Cuts at: 3225 3225 Size: 5944 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5944 NotI GC'GGCC_GC Cuts at: 3391 3391 Size: 5944 NsiI A_TGCA'T Cuts at: 2727 2727 Size: 5944 NspI r_CATG'y Cuts at: 1132 1229 2833 3302 4847 1132 Size: 97 1604 469 1545 2229 Fragments arranged by size: 2229 1604 1545 469 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5944 PciI A'CATG_T Cuts at: 2829 4843 2829 Size: 2014 3930 PflMI CCAn_nnn'nTGG Cuts at: 1108 2397 2717 3108 1108 Size: 1289 320 391 3944 Fragments arranged by size: 3944 1289 391 320 PfoI T'CCnGG_A Cuts at: 2184 2184 Size: 5944 PmeI GTTT'AAAC Cuts at: 3248 3248 Size: 5944 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4013 4045 5526 5558 4013 Size: 32 1481 32 4399 Fragments arranged by size: 4399 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5944 PsiI TTA'TAA Cuts at: 3514 3514 Size: 5944 PstI C_TGCA'G Cuts at: 830 3296 830 Size: 2466 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5944 SalI G'TCGA_C Cuts at: 1936 3286 1936 Size: 1350 4594 SbfI CC_TGCA'GG Cuts at: 3296 3296 Size: 5944 ScaI AGT'ACT Cuts at: 3131 4192 5904 3131 Size: 1061 1712 3171 Fragments arranged by size: 3171 1712 1061 SexAI A'CCwGG_T Cuts at: 2716 2716 Size: 5944 SfcI C'TryA_G Cuts at: 826 1045 3292 4427 5108 5299 826 Size: 219 2247 1135 681 191 1471 Fragments arranged by size: 2247 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5944 SmaI CCC'GGG Cuts at: 1872 3271 1872 Size: 1399 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4048 4949 5211 820 Size: 197 926 2105 901 262 1553 Fragments arranged by size: 2105 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3735 493 Size: 3242 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5944 SphI G_CATG'C Cuts at: 1132 3302 1132 Size: 2170 3774 SspI AAT'ATT Cuts at: 5 52 3868 5 Size: 47 3816 2081 Fragments arranged by size: 3816 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5944 StyI C'CwwG_G Cuts at: 513 1065 2391 3148 3383 513 Size: 552 1326 757 235 3074 Fragments arranged by size: 3074 1326 757 552 235 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2044 2455 3971 3988 4141 4326 4771 Size: 1255 464 411 1516 17 153 185 445 Cuts at: 4771 325 Size: 1498 Fragments arranged by size: 1516 1498 1255 464 445 411 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2847 3129 4190 Size: 274 80 33 51 1443 870 282 1061 Cuts at: 4190 5902 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1061 870 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5904 5936 5904 Size: 32 5912 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5944 XbaI T'CTAG_A Cuts at: 3280 3280 Size: 5944 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2368 2011 Size: 357 5587 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5944 XmaI C'CCGG_G Cuts at: 1870 3269 1870 Size: 1399 4545 XmnI GAAnn'nnTTC Cuts at: 2747 4073 2747 Size: 1326 4618 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3752 276 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI