################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MaeI MamI MfeI MflI MluI MroI MslI MunI MvaI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcyI 180 GGCGTC G[GA]^CG[CT]C AluI 263 AGCT AG^CT AlwNI 188 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 461 TGGCCA TGG^CCA BanII 76 GGGCTC G[GA]GC[CT]^C BbvCI 185 CCTCAGC CCTCAGC(-5/-2) BmyI 76 GGGCTC G[AGT]GC[ACT]^C BpmI 217 CTGGAG CTGGAG(16/14) BsaHI 180 GGCGTC G[GA]^CG[CT]C Bsp1286I 76 GGGCTC G[AGT]GC[ACT]^C BstF5I 375 GGATG GGATG(2/0) DdeI 186 CTCAG C^T[ACGT]AG DpnII 447 GATC ^GATC EarI 520 GAAGAG CTCTTC(1/4) EcoRI 525 GAATTC G^AATTC FokI 375 GGATG GGATG(9/13) HaeII 207 AGCGCC [GA]GCGC^[CT] HgaI 181 GCGTC GACGC(5/10) Hin1I 180 GGCGTC G[GA]^CG[CT]C HphI 320 TCACC GGTGA(8/7) Ksp632I 520 GAAGAG CTCTTC(1/4) MaeII 458 ACGT A^CGT MaeIII 319 GTCAC ^GT[ACGT]AC MboI 447 GATC ^GATC MluNI 461 TGGCCA TGG^CCA MscI 461 TGGCCA TGG^CCA MseI 452 TTAA T^TAA NdeII 447 GATC ^GATC PstI 488 CTGCAG CTGCA^G PvuII 262 CAGCTG CAG^CTG Sau3AI 447 GATC ^GATC ScaI 368 AGTACT AGT^ACT SfcI 488 CTGCAG C^T[GA][CT]AG SmaI 73 CCCGGG CCC^GGG Tru9I 452 TTAA T^TAA Tsp45I 319 GTCAC ^GT[GC]AC XmaCI 73 CCCGGG C^CCGGG XmaI 73 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 466 AAATTC [GA]^AATT[CT] 525 GAATTC AfaI 237 GTAC GT^AC 369 GTAC ApoI 466 AAATTC [GA]^AATT[CT] 525 GAATTC AvaI 34 CCCGAG C^[CT]CG[GA]G 73 CCCGGG AvaII 30 GGACC G^G[AT]CC 69 GGTCC BbvI 261 GCAGC GCAGC(8/12) 90 GCTGC BsaI 496 GAGACC GGTCTC(1/5) 148 GGTCTC BsmAI 496 GAGAC GTCTC(1/5) 149 GTCTC BsoBI 34 CCCGAG C^[CT]CG[GA]G 73 CCCGGG BsrFI 43 GCCGGC [GA]^CCGG[CT] 93 GCCGGC Cfr10I 43 GCCGGC [GA]^CCGG[CT] 93 GCCGGC DsaI 65 CCGCGG C^C[GA][CT]GG 323 CCGCGG FauI 64 CCCGC CCCGC(4/6) 99 GCGGG KspI 65 CCGCGG CCGC^GG 323 CCGCGG MboII 517 GAAGA GAAGA(8/7) 520 GAAGA NaeI 43 GCCGGC GCC^GGC 93 GCCGGC NgoMIV 43 GCCGGC G^CCGGC 93 GCCGGC NlaIII 258 CATG CATG^ 297 CATG RsaI 237 GTAC GT^AC 369 GTAC SacII 65 CCGCGG CCGC^GG 323 CCGCGG SfaNI 253 GATGC GCATC(5/9) 190 GCATC TseI 90 GCTGC G^C[AT]GC 261 GCAGC Tsp509I 467 AATT ^AATT 526 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AccII AciI BcnI BsaJI BsiYI BslI BsmFI BssKI BstUI Cac8I CfoI Cfr13I EaeI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MnlI MspA1I MspI MvnI MwoI NciI NlaIV PleI Sau96I ScrFI