(Circular) (Six-base) MAPSORT of: pFN21AE3042.seq Check: 2692 from: 1 to: 5251 pFN21AE3042 5251 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3061 278 Size: 53 83 186 936 1525 2468 Fragments arranged by size: 2468 1525 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2594 1937 Size: 657 4594 Acc65I G'GTAC_C Cuts at: 2572 2572 Size: 5251 AclI AA'CG_TT Cuts at: 1563 3378 3751 1563 Size: 1815 373 3063 Fragments arranged by size: 3063 1815 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3314 4698 4966 1632 Size: 1682 1384 268 1917 Fragments arranged by size: 1917 1682 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5054 AflIII A'CryG_T Cuts at: 1203 4150 5127 1203 Size: 2947 977 1327 Fragments arranged by size: 2947 1327 977 AgeI A'CCGG_T Cuts at: 4082 4082 Size: 5251 AhdI GACnn_n'nnGTC Cuts at: 3980 3980 Size: 5251 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5251 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5251 AlwNI CAG_nnn'CTG Cuts at: 2198 2994 4566 2198 Size: 796 1572 2883 Fragments arranged by size: 2883 1572 796 ApaLI G'TGCA_C Cuts at: 3308 4464 3308 Size: 1156 4095 ApoI r'AATT_y Cuts at: 1493 2471 2530 2560 2786 2938 5047 1493 Size: 978 59 30 226 152 2109 1697 Fragments arranged by size: 2109 1697 978 226 152 59 30 AseI AT'TA_AT Cuts at: 160 1032 3805 160 Size: 872 2773 1606 Fragments arranged by size: 2773 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5251 AvaI C'yCGr_G Cuts at: 1870 1943 2039 2078 2576 1870 Size: 73 96 39 498 4545 Fragments arranged by size: 4545 498 96 73 39 BamHI G'GATC_C Cuts at: 2581 2948 2581 Size: 367 4884 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2572 4027 618 Size: 325 217 627 785 1455 1842 Fragments arranged by size: 1842 1455 785 627 325 217 BanII G_rGCy'C Cuts at: 1391 2085 2570 1391 Size: 694 485 4072 Fragments arranged by size: 4072 694 485 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4280 BbvCI CC'TCA_GC Cuts at: 2191 2191 Size: 5251 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3442 3476 1903 Size: 34 1505 34 3678 Fragments arranged by size: 3678 1505 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3143 4353 3143 Size: 1210 4041 BclI T'GATC_A Cuts at: 1548 1548 Size: 5251 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3862 136 Size: 107 122 71 631 605 2190 1525 Fragments arranged by size: 2190 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5246 5246 Size: 5251 BlpI GC'TnA_GC Cuts at: 2668 2668 Size: 5251 Bme1580I G_kGCm'C Cuts at: 1790 3312 4468 1790 Size: 1522 1156 2573 Fragments arranged by size: 2573 1522 1156 BmgBI CAC'GTC Cuts at: 1318 2464 1318 Size: 1146 4105 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2982 3940 442 Size: 416 53 83 399 24 1565 958 1753 Fragments arranged by size: 1753 1565 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5251 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2243 3911 1239 Size: 1004 1668 2579 Fragments arranged by size: 2579 1668 1004 Bpu10I CC'TnA_GC Cuts at: 2191 2191 Size: 5251 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3376 4241 4539 3376 Size: 865 298 4088 Fragments arranged by size: 4088 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2159 2495 3914 882 Size: 617 660 336 1419 2219 Fragments arranged by size: 2219 1419 660 617 336 BsaAI yAC'GTr Cuts at: 493 1204 3042 493 Size: 711 1838 2702 Fragments arranged by size: 2702 1838 711 BsaBI GATnn'nnATC Cuts at: 5245 5245 Size: 5251 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2186 3058 3440 4075 Size: 53 83 186 936 653 872 382 635 Cuts at: 4075 275 Size: 1451 Fragments arranged by size: 1451 936 872 653 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3684 4082 4356 4503 4914 3684 Size: 398 274 147 411 4021 Fragments arranged by size: 4021 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5026 Fragments arranged by size: 5026 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5251 BseYI C'CCAG_C Cuts at: 2399 4454 4950 2399 Size: 2055 496 2700 Fragments arranged by size: 2700 2055 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4678 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2701 3462 4490 1540 Size: 275 186 700 761 1028 2301 Fragments arranged by size: 2301 1028 761 700 275 186 BsiHKAI G_wGCw'C Cuts at: 3312 3397 4468 3312 Size: 85 1071 4095 Fragments arranged by size: 4095 1071 85 BsmI GAATG_Cn' Cuts at: 2762 2855 2762 Size: 93 5158 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2570 3312 3397 4468 1391 Size: 399 295 485 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 485 399 295 85 BspEI T'CCGG_A Cuts at: 4914 4914 Size: 5251 BspHI T'CATG_A Cuts at: 3140 4870 3140 Size: 1730 3521 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2606 844 Size: 1762 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3138 1394 Size: 429 1315 3507 Fragments arranged by size: 3507 1315 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3746 3920 65 Size: 1183 2498 174 1396 Fragments arranged by size: 2498 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 2098 3895 4082 1700 Size: 110 238 50 1797 187 2869 Fragments arranged by size: 2869 1797 238 187 110 50 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5251 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5251 BssSI C'ACGA_G Cuts at: 3311 4323 3311 Size: 1012 4239 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5251 BstBI TT'CG_AA Cuts at: 4055 4055 Size: 5251 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5251 BstYI r'GATC_y Cuts at: 1972 2581 2948 3333 3350 4802 4888 4900 5246 Size: 609 367 385 17 1452 86 12 346 Cuts at: 5246 1972 Size: 1977 Fragments arranged by size: 1977 1452 609 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2070 2328 513 Size: 552 1005 258 3436 Fragments arranged by size: 3436 1005 552 258 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2350 487 Size: 683 56 1124 3388 Fragments arranged by size: 3388 1124 683 56 BtsI GCAGTG_nn' Cuts at: 2767 3561 3581 2767 Size: 794 20 4437 Fragments arranged by size: 4437 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5251 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5216 DraI TTT'AAA Cuts at: 2555 2555 Size: 5251 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4258 809 Size: 507 2942 1802 Fragments arranged by size: 2942 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2361 2466 Size: 54 1425 50 275 18 312 219 105 Cuts at: 2466 2698 3587 5216 8 Size: 232 889 1629 43 Fragments arranged by size: 1629 1425 889 312 275 232 219 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2698 1537 Size: 275 886 4090 Fragments arranged by size: 4090 886 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2520 3181 1353 Size: 1167 661 3423 Fragments arranged by size: 3423 1167 661 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3838 4212 4358 387 Size: 1064 138 2249 374 146 1280 Fragments arranged by size: 2249 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 3314 3911 4698 4966 1239 Size: 393 611 1071 597 787 268 1524 Fragments arranged by size: 1524 1071 787 611 597 393 268 EcoRI G'AATT_C Cuts at: 2530 2560 2938 2530 Size: 30 378 4843 Fragments arranged by size: 4843 378 30 FspI TGC'GCA Cuts at: 3757 3757 Size: 5251 HaeII r_GCGC'y Cuts at: 1678 2216 4398 1678 Size: 538 2182 2531 Fragments arranged by size: 2531 2182 538 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2528 2560 3892 3924 3966 Size: 32 135 32 956 32 1332 32 42 Cuts at: 3966 3998 1373 Size: 32 2626 Fragments arranged by size: 2626 1332 956 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2595 2841 669 Size: 988 281 657 246 3079 Fragments arranged by size: 3079 988 657 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5251 HpaI GTT'AAC Cuts at: 2841 2841 Size: 5251 KpnI G_GTAC'C Cuts at: 2576 2576 Size: 5251 MfeI C'AATT_G Cuts at: 2850 2850 Size: 5251 MluI A'CGCG_T Cuts at: 5127 5127 Size: 5251 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2559 4365 4549 1305 Size: 1254 1806 184 2007 Fragments arranged by size: 2007 1806 1254 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2468 10 Size: 54 1425 343 636 2793 Fragments arranged by size: 2793 1425 636 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3209 3568 3727 518 Size: 648 2043 359 159 2042 Fragments arranged by size: 2043 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2072 2269 2330 2399 2667 3344 4492 4737 Size: 466 197 61 69 268 677 1148 245 Cuts at: 4737 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 466 268 245 197 69 61 NaeI GCC'GGC Cuts at: 2050 2100 2050 Size: 50 5201 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4699 NdeI CA'TA_TG Cuts at: 387 387 Size: 5251 NgoMIV G'CCGG_C Cuts at: 2048 2098 2048 Size: 50 5201 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5251 NotI GC'GGCC_GC Cuts at: 2698 2698 Size: 5251 NspI r_CATG'y Cuts at: 1132 1229 2609 4154 1132 Size: 97 1380 1545 2229 Fragments arranged by size: 2229 1545 1380 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5251 PciI A'CATG_T Cuts at: 4150 4150 Size: 5251 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5251 PmeI GTTT'AAAC Cuts at: 2555 2555 Size: 5251 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3320 3352 4833 4865 3320 Size: 32 1481 32 3706 Fragments arranged by size: 3706 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5251 PsiI TTA'TAA Cuts at: 2821 2821 Size: 5251 PstI C_TGCA'G Cuts at: 830 2497 2603 830 Size: 1667 106 3478 Fragments arranged by size: 3478 1667 106 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5251 PvuII CAG'CTG Cuts at: 2269 2269 Size: 5251 SacII CC_GC'GG Cuts at: 2073 2331 2073 Size: 258 4993 SalI G'TCGA_C Cuts at: 1936 2593 1936 Size: 657 4594 SbfI CC_TGCA'GG Cuts at: 2603 2603 Size: 5251 ScaI AGT'ACT Cuts at: 2375 3499 5211 2375 Size: 1124 1712 2415 Fragments arranged by size: 2415 1712 1124 SfcI C'TryA_G Cuts at: 826 1045 2493 2599 3734 4415 4606 826 Size: 219 1448 106 1135 681 191 1471 Fragments arranged by size: 1471 1448 1135 681 219 191 106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5251 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5251 SmaI CCC'GGG Cuts at: 1872 2080 2578 1872 Size: 208 498 4545 Fragments arranged by size: 4545 498 208 SmlI C'TyrA_G Cuts at: 820 1017 1943 3355 4256 4518 820 Size: 197 926 1412 901 262 1553 Fragments arranged by size: 1553 1412 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3042 493 Size: 2549 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5251 SphI G_CATG'C Cuts at: 1132 2609 1132 Size: 1477 3774 SspI AAT'ATT Cuts at: 5 52 3175 5 Size: 47 3123 2081 Fragments arranged by size: 3123 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5251 StyI C'CwwG_G Cuts at: 513 1065 2690 513 Size: 552 1625 3074 Fragments arranged by size: 3074 1625 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3278 3295 3448 3633 4078 325 Size: 1255 1698 17 153 185 445 1498 Fragments arranged by size: 1698 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 2373 3497 Size: 274 80 33 51 1443 264 132 1124 Cuts at: 3497 5209 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1124 274 264 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5211 5243 5211 Size: 32 5219 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5251 XbaI T'CTAG_A Cuts at: 2587 2587 Size: 5251 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5251 XmaI C'CCGG_G Cuts at: 1870 2078 2576 1870 Size: 208 498 4545 Fragments arranged by size: 4545 498 208 XmnI GAAnn'nnTTC Cuts at: 3380 3380 Size: 5251 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3059 276 Size: 53 83 186 936 1525 2468 Fragments arranged by size: 2468 1525 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI