################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccII AclI AflIII AgeI AlwNI AocI Aor51HI ApaI AscI AseI AsnI AspI AviII AvrII BamHI BanII BbrPI BbvCI BcgI BciVI BclI BcnI BglII BlnI BsaAI BsaBI BsaI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HindIII HpaI KspI MamI MfeI MluI MunI MvnI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### Acc65I 1156 GGTACC G^GTACC AccI 103 GTCGAC GT^[AC][GT]AC AccIII 701 TCCGGA T^CCGGA AciI 199 GCGG CCGC(-3/-1) AcyI 410 GGCGCC G[GA]^CG[CT]C AflII 676 CTTAAG C^TTAAG AhdI 106 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 546 GTGCAC G^TGCAC ApaLI 546 GTGCAC G^TGCAC Asp700I 693 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1156 GGTACC G^GTACC AspEI 106 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 546 GTGCAC G[AT]GC[AT]^C AvaII 720 GGTCC G^G[AT]CC BalI 1134 TGGCCA TGG^CCA BbeI 410 GGCGCC GGCGC^C BbsI 1057 GAAGAC GAAGAC(2/6) BfrI 676 CTTAAG C^TTAAG BglI 402 GCCTGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 451 GCTAAGC GC^T[ACGT]AGC Bpu1102I 451 GCTAAGC GC^T[ACGT]AGC BpuAI 1057 GAAGAC GAAGAC(2/6) BsaHI 410 GGCGCC G[GA]^CG[CT]C BsaWI 701 TCCGGA [AT]^CCGG[AT] BseAI 701 TCCGGA T^CCGGA BsiEI 105 CGACCG CG[GA][CT]^CG BsiHKAI 546 GTGCAC G[AT]GC[AT]^C BsmAI 798 GAGAC GTCTC(1/5) BspEI 701 TCCGGA T^CCGGA BspHI 1256 TCATGA T^CATGA BstYI 1248 GGATCT [GA]^GATC[CT] CelII 451 GCTAAGC GC^T[ACGT]AGC DsaI 108 CCGTGG C^C[GA][CT]GG EaeI 1134 TGGCCA [CT]^GGCC[GA] Eam1105I 106 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 863 CCAAGG C^C[AT][AT]GG HapII 702 CCGG C^CGG HgaI 312 GCGTC GACGC(5/10) Hin1I 410 GGCGCC G[GA]^CG[CT]C HpaII 702 CCGG C^CGG HphI 559 GGTGA GGTGA(8/7) KasI 410 GGCGCC G^GCGCC KpnI 1156 GGTACC GGTAC^C MflI 1248 GGATCT [GA]^GATC[CT] MluNI 1134 TGGCCA TGG^CCA MroI 701 TCCGGA T^CCGGA MscI 1134 TGGCCA TGG^CCA MseI 677 TTAA T^TAA MslI 1257 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 702 CCGG C^CGG NarI 410 GGCGCC GG^CGCC NspI 327 ACATGC [GA]CATG^[CT] PpuMI 719 AGGTCCC [GA]G^G[AT]CC[CT] PstI 1111 CTGCAG CTGCA^G RcaI 1256 TCATGA T^CATGA SalI 103 GTCGAC G^TCGAC SfoI 410 GGCGCC GGC^GCC StyI 863 CCAAGG C^C[AT][AT]GG TaqI 104 TCGA T^CGA Tru9I 677 TTAA T^TAA TthHB8I 104 TCGA T^CGA XbaI 982 TCTAGA T^CTAGA XhoII 1248 GGATCT [GA]^GATC[CT] XmnI 693 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 95 AAATTC [GA]^AATT[CT] 1178 AAATTC AlwI 601 GATCC GGATC(4/5) 1248 GGATC ApoI 95 AAATTC [GA]^AATT[CT] 1178 AAATTC AvaI 10 CCCGAG C^[CT]CG[GA]G 909 CTCGGG BmyI 6 GTGCCC G[AGT]GC[ACT]^C 546 GTGCAC BseRI 1069 GAGGAG GAGGAG(10/8) 1072 GAGGAG BsiYI 924 CCACAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1137 CCAGTATAGGG BslI 924 CCACAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1137 CCAGTATAGGG BsoBI 10 CCCGAG C^[CT]CG[GA]G 909 CTCGGG Bsp1286I 6 GTGCCC G[AGT]GC[ACT]^C 546 GTGCAC BsrDI 144 CATTGC GCAATG(2/0) 846 CATTGC Cac8I 148 GCTGGC GC[ACGT]^[ACGT]GC 1113 GCAGGC Cfr13I 228 GGCCC G^G[ACGT]CC 720 GGTCC DpnII 601 GATC ^GATC 1249 GATC DraII 227 AGGCCCC [GA]G^G[ACGT]CC[CT] 719 AGGTCCC EarI 1066 GAAGAG CTCTTC(1/4) 1090 GAAGAG EcoO109I 227 AGGCCCC [GA]G^G[ACGT]CC[CT] 719 AGGTCCC EcoRV 562 GATATC GAT^ATC 903 GATATC HincII 103 GTCGAC GT[CT]^[GA]AC 460 GTTGAC HindII 103 GTCGAC GT[CT]^[GA]AC 460 GTTGAC Ksp632I 1066 GAAGAG CTCTTC(1/4) 1090 GAAGAG MaeII 354 ACGT A^CGT 779 ACGT MboI 601 GATC ^GATC 1249 GATC MspA1I 587 CAGCTG C[AC]G^C[GT]G 1108 CAGCTG NdeII 601 GATC ^GATC 1249 GATC PvuII 587 CAGCTG CAG^CTG 1108 CAGCTG Sau3AI 601 GATC ^GATC 1249 GATC Sau96I 228 GGCCC G^G[ACGT]CC 720 GGTCC SfcI 1111 CTGCAG C^T[GA][CT]AG 1354 CTACAG TspRI 549 CACTG CA[GC]TG[ACGT][ACGT]^ 975 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AfaI AluI BanI BbvI BfaI BpmI BsaJI BsmFI BsrI BssKI BstF5I BstNI CfoI DdeI EcoRII Fnu4HI FokI HaeII HaeIII HhaI HinP1I HinfI ItaI MaeI MaeIII MboII MnlI MvaI MwoI NlaIII NlaIV RsaI ScrFI SfaNI SmlI TfiI TseI Tsp509I