(Circular) (Six-base) MAPSORT of: pFN21AB7704.seq Check: 5277 from: 1 to: 6091 pFN21AB7704 6091 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2109 3434 1937 Size: 172 1325 4594 Fragments arranged by size: 4594 1325 172 Acc65I G'GTAC_C Cuts at: 3161 3412 3161 Size: 251 5840 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2022 2844 2877 3114 3404 4154 5538 5806 Size: 390 822 33 237 290 750 1384 268 Cuts at: 5806 1632 Size: 1917 Fragments arranged by size: 1917 1384 822 750 390 290 268 237 33 AflII C'TTAA_G Cuts at: 820 1017 2681 820 Size: 197 1664 4230 Fragments arranged by size: 4230 1664 197 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 2116 4820 2116 Size: 2704 3387 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 3834 5406 3834 Size: 1572 4519 ApaLI G'TGCA_C Cuts at: 2551 4148 5304 2551 Size: 1597 1156 3338 Fragments arranged by size: 3338 1597 1156 ApoI r'AATT_y Cuts at: 1493 2100 3183 3400 3626 3778 5887 1493 Size: 607 1083 217 226 152 2109 1697 Fragments arranged by size: 2109 1697 1083 607 226 217 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2914 3416 1870 Size: 73 72 899 502 4545 Fragments arranged by size: 4545 899 502 73 72 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2165 2415 3161 3412 4867 Size: 325 217 627 378 250 746 251 1455 Cuts at: 4867 618 Size: 1842 Fragments arranged by size: 1842 1455 746 627 378 325 251 250 217 BanII G_rGCy'C Cuts at: 1391 3410 1391 Size: 2019 4072 BbeI G_GCGC'C Cuts at: 2419 2419 Size: 6091 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3069 928 Size: 971 1170 3950 Fragments arranged by size: 3950 1170 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2413 4702 136 Size: 107 122 71 631 605 741 2289 1525 Fragments arranged by size: 2289 1525 741 631 605 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 2457 3508 2457 Size: 1051 5040 Bme1580I G_kGCm'C Cuts at: 1790 2015 2555 4152 5308 1790 Size: 225 540 1597 1156 2573 Fragments arranged by size: 2573 1597 1156 540 225 BmgBI CAC'GTC Cuts at: 1318 2785 1318 Size: 1467 4624 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2565 2763 3329 4751 1239 Size: 1326 198 566 1422 2579 Fragments arranged by size: 2579 1422 1326 566 198 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3269 3338 4216 5081 5379 3269 Size: 69 878 865 298 3981 Fragments arranged by size: 3981 878 865 298 69 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4754 882 Size: 617 3255 2219 Fragments arranged by size: 3255 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2416 3898 4280 4915 Size: 53 83 186 936 883 1482 382 635 Cuts at: 4915 275 Size: 1451 Fragments arranged by size: 1482 1451 936 883 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2706 4524 4922 5196 5343 5754 2706 Size: 1818 398 274 147 411 3043 Fragments arranged by size: 3043 1818 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5866 Fragments arranged by size: 5866 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3089 3092 1186 Size: 1903 3 4185 Fragments arranged by size: 4185 1903 3 BseYI C'CCAG_C Cuts at: 5294 5790 5294 Size: 496 5595 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5518 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2113 3541 4302 5330 1540 Size: 275 186 112 1428 761 1028 2301 Fragments arranged by size: 2301 1428 1028 761 275 186 112 BsiHKAI G_wGCw'C Cuts at: 2555 4152 4237 5308 2555 Size: 1597 85 1071 3338 Fragments arranged by size: 3338 1597 1071 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2555 3410 4152 4237 5308 1391 Size: 399 225 540 855 742 85 1071 2174 Fragments arranged by size: 2174 1071 855 742 540 399 225 85 BspEI T'CCGG_A Cuts at: 2706 5754 2706 Size: 3048 3043 BspHI T'CATG_A Cuts at: 3261 3980 5710 3261 Size: 719 1730 3642 Fragments arranged by size: 3642 1730 719 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3446 844 Size: 2602 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3978 1394 Size: 429 2155 3507 Fragments arranged by size: 3507 2155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2148 2850 4586 4760 65 Size: 1183 900 702 1736 174 1396 Fragments arranged by size: 1736 1396 1183 900 702 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 4922 1700 Size: 110 2925 187 2869 Fragments arranged by size: 2925 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6091 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstYI r'GATC_y Cuts at: 1972 3253 3421 3788 4173 4190 5642 5728 5740 Size: 1281 168 367 385 17 1452 86 12 Cuts at: 5740 6086 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1281 385 367 346 168 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2113 513 Size: 552 939 109 4491 Fragments arranged by size: 4491 939 552 109 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 2553 3607 4401 4421 2553 Size: 1054 794 20 4223 Fragments arranged by size: 4223 1054 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3139 3538 4427 Size: 54 1425 50 275 18 1309 399 889 Cuts at: 4427 6056 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1309 889 399 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3066 3090 4021 1353 Size: 1713 24 931 3423 Fragments arranged by size: 3423 1713 931 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4678 5052 5198 387 Size: 1064 138 3089 374 146 1280 Fragments arranged by size: 3089 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2022 2565 2763 2844 2877 3114 3329 Size: 393 390 543 198 81 33 237 215 Cuts at: 3329 3404 4154 4751 5538 5806 1239 Size: 75 750 597 787 268 1524 Fragments arranged by size: 1524 787 750 597 543 393 390 268 237 215 198 81 75 33 EcoO109I rG'GnC_Cy Cuts at: 2233 2725 2233 Size: 492 5599 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 EcoRV GAT'ATC Cuts at: 2569 2910 2569 Size: 341 5750 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 2145 2217 2419 5238 1678 Size: 467 72 202 2819 2531 Fragments arranged by size: 2819 2531 467 202 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 4732 4764 4806 Size: 32 135 32 530 32 2598 32 42 Cuts at: 4806 4838 1373 Size: 32 2626 Fragments arranged by size: 2626 2598 530 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2110 2467 3435 3681 669 Size: 988 281 172 357 968 246 3079 Fragments arranged by size: 3079 988 968 357 281 246 172 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KasI G'GCGC_C Cuts at: 2415 2415 Size: 6091 KpnI G_GTAC'C Cuts at: 3165 3416 3165 Size: 251 5840 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5205 5389 1305 Size: 3900 184 2007 Fragments arranged by size: 3900 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3141 10 Size: 54 1425 343 1309 2960 Fragments arranged by size: 2960 1425 1309 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3266 4049 4408 4567 518 Size: 648 2100 783 359 159 2042 Fragments arranged by size: 2100 2042 783 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2594 3115 3507 4184 5332 5577 1606 Size: 988 521 392 677 1148 245 2120 Fragments arranged by size: 2120 1148 988 677 521 392 245 NarI GG'CG_CC Cuts at: 2416 2416 Size: 6091 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4600 Fragments arranged by size: 4600 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2336 3449 4994 1132 Size: 97 1107 1113 1545 2229 Fragments arranged by size: 2229 1545 1113 1107 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PfoI T'CCnGG_A Cuts at: 2247 2247 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2725 2725 Size: 6091 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 2520 3661 2520 Size: 1141 4950 PstI C_TGCA'G Cuts at: 830 3120 3443 830 Size: 2290 323 3478 Fragments arranged by size: 3478 2290 323 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2594 3115 2594 Size: 521 5570 SalI G'TCGA_C Cuts at: 1936 2108 3433 1936 Size: 172 1325 4594 Fragments arranged by size: 4594 1325 172 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SfcI C'TryA_G Cuts at: 826 1045 3116 3359 3439 4574 5255 5446 826 Size: 219 2071 243 80 1135 681 191 1471 Fragments arranged by size: 2071 1471 1135 681 243 219 191 80 SfoI GGC'GCC Cuts at: 2417 2417 Size: 6091 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2681 3284 3317 4195 5096 5358 Size: 197 926 738 603 33 878 901 262 Cuts at: 5358 820 Size: 1553 Fragments arranged by size: 1553 926 901 878 738 603 262 197 33 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2004 2868 3530 513 Size: 552 939 864 662 3074 Fragments arranged by size: 3074 939 864 662 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2183 4118 4135 4288 4473 4918 325 Size: 1255 603 1935 17 153 185 445 1498 Fragments arranged by size: 1935 1498 1255 603 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2339 2766 4337 Size: 274 80 33 51 1443 362 427 1571 Cuts at: 4337 6049 96 Size: 1712 138 Fragments arranged by size: 1712 1571 1443 427 362 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2158 2190 6051 6083 2158 Size: 32 3861 32 2166 Fragments arranged by size: 3861 2166 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 2987 3427 2987 Size: 440 5651 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 2702 4220 2702 Size: 1518 4573 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI