################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanII BbrPI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoRI EcoT22I FspI HincII HindII HpaI KpnI KspI MaeII MfeI MluI MroI MslI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccII 1064 CGCG CG^CG AcsI 449 AAATTC [GA]^AATT[CT] AfaI 621 GTAC GT^AC AlwNI 221 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1017 CCTGAGG CC^T[ACGT]AGG Aor51HI 637 AGCGCT AGC^GCT ApoI 449 AAATTC [GA]^AATT[CT] AspHI 859 GTGCTC G[AT]GC[AT]^C AvaI 1090 CTCGGG C^[CT]CG[GA]G BalI 1010 TGGCCA TGG^CCA BanI 455 GGCGCC G^G[CT][GA]CC BbeI 455 GGCGCC GGCGC^C BbsI 1042 GTCTTC GAAGAC(2/6) BbvCI 1186 GCTGAGG CCTCAGC(-5/-2) BclI 263 TGATCA T^GATCA BglI 107 GCCCAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1042 GTCTTC GAAGAC(2/6) BsaBI 150 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 863 TCCGGT [AT]^CCGG[AT] BseRI 610 CTCCTC GAGGAG(10/8) BsgI 1172 GTGCAG GTGCAG(16/14) BsiHKAI 859 GTGCTC G[AT]GC[AT]^C BsmBI 178 GAGACG CGTCTC(1/5) BsmI 1220 GCATTC GAATGC(1/-1) BsoBI 1090 CTCGGG C^[CT]CG[GA]G BspHI 908 TCATGA T^CATGA BspMI 829 ACCTGC ACCTGC(4/8) BstEII 1248 GGTCACC G^GT[ACGT]ACC BstPI 1248 GGTCACC G^GT[ACGT]ACC BstUI 1064 CGCG CG^CG BstXI 435 CCAATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1017 CCTGAGG CC^T[ACGT]AGG Cac8I 116 GCCGGC GC[ACGT]^[ACGT]GC DraIII 1314 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 637 AGCGCT AGC^GCT Eco81I 1017 CCTGAGG CC^T[ACGT]AGG EcoO65I 1248 GGTCACC G^GT[ACGT]ACC EcoRV 478 GATATC GAT^ATC FbaI 263 TGATCA T^GATCA FseI 115 GGCCGGCC GGCCGG^CC HgaI 1108 GCGTC GACGC(5/10) HindIII 193 AAGCTT A^AGCTT KasI 455 GGCGCC G^GCGCC MamI 150 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1010 TGGCCA TGG^CCA MscI 1010 TGGCCA TGG^CCA MseI 1490 TTAA T^TAA MspA1I 256 CAGCGG C[AC]G^C[GT]G MvnI 1064 CGCG CG^CG NaeI 116 GCCGGC GCC^GGC NarI 455 GGCGCC GG^CGCC NcoI 524 CCATGG C^CATGG NgoMIV 116 GCCGGC G^CCGGC PpuMI 1334 GGGTCCT [GA]G^G[AT]CC[CT] PstI 1081 CTGCAG CTGCA^G RcaI 908 TCATGA T^CATGA RsaI 621 GTAC GT^AC SapI 70 GAAGAGC GCTCTTC(1/4) SfoI 455 GGCGCC GGC^GCC SmlI 1464 CTCAAG C^T[CT][GA]AG Tru9I 1490 TTAA T^TAA Tsp45I 1249 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 825 GTCTAC GT^[AC][GT]AC 1177 GTCTAC AcyI 455 GGCGCC G[GA]^CG[CT]C 1107 GGCGTC AflIII 1001 ACATGT A^C[GA][CT]GT 1277 ACATGT BpmI 1167 CTCCAG CTGGAG(16/14) 703 CTGGAG BsaHI 455 GGCGCC G[GA]^CG[CT]C 1107 GGCGTC BspLU11I 1001 ACATGT A^CATGT 1277 ACATGT BsrFI 116 GCCGGC [GA]^CCGG[CT] 401 ACCGGC BstYI 149 AGATCC [GA]^GATC[CT] 1085 AGATCC Cfr10I 116 GCCGGC [GA]^CCGG[CT] 401 ACCGGC DraII 222 AGGGCCT [GA]G^G[ACGT]CC[CT] 1334 GGGTCCT DsaI 524 CCATGG C^C[GA][CT]GG 656 CCGTGG EaeI 653 TGGCCG [CT]^GGCC[GA] 1010 TGGCCA EcoO109I 222 AGGGCCT [GA]G^G[ACGT]CC[CT] 1334 GGGTCCT EcoT14I 524 CCATGG C^C[AT][AT]GG 1073 CCAAGG FauI 258 GCGGG CCCGC(4/6) 1116 GCGGG HaeII 455 GGCGCC [GA]GCGC^[CT] 637 AGCGCT Hin1I 455 GGCGCC G[GA]^CG[CT]C 1107 GGCGTC MaeIII 398 GTAAC ^GT[ACGT]AC 1249 GTCAC MflI 149 AGATCC [GA]^GATC[CT] 1085 AGATCC MwoI 107 GCCCAAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 640 GCTGCCCGTGC NspI 1001 ACATGT [GA]CATG^[CT] 1277 ACATGT SfcI 1081 CTGCAG C^T[GA][CT]AG 1262 CTGTAG StyI 524 CCATGG C^C[AT][AT]GG 1073 CCAAGG TaqI 95 TCGA T^CGA 851 TCGA TfiI 419 GAATC G^A[AT]TC 1445 GAATC TthHB8I 95 TCGA T^CGA 851 TCGA XcmI 692 CCATCTTCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1112 CCATGCGGGAGTTGG XhoII 149 AGATCC [GA]^GATC[CT] 1085 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BcnI BfaI BmyI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MboI MboII MnlI MspI MvaI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TseI Tsp509I TspRI