(Circular) (Six-base) MAPSORT of: pFN21AE4123.seq Check: 1184 from: 1 to: 6238 pFN21AE4123 6238 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2831 3183 3581 1937 Size: 894 352 398 4594 Fragments arranged by size: 4594 894 398 352 Acc65I G'GTAC_C Cuts at: 3559 3559 Size: 6238 AclI AA'CG_TT Cuts at: 1563 4365 4738 1563 Size: 2802 373 3063 Fragments arranged by size: 3063 2802 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2765 2887 3128 4301 5685 5953 1632 Size: 1133 122 241 1173 1384 268 1917 Fragments arranged by size: 1917 1384 1173 1133 268 241 122 AfeI AGC'GCT Cuts at: 2644 2644 Size: 6238 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 3006 3282 5137 6114 1203 Size: 1803 276 1855 977 1327 Fragments arranged by size: 1855 1803 1327 977 276 AgeI A'CCGG_T Cuts at: 5069 5069 Size: 6238 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6238 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6238 AlwNI CAG_nnn'CTG Cuts at: 2231 3981 5553 2231 Size: 1750 1572 2916 Fragments arranged by size: 2916 1750 1572 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 2454 3547 3773 3925 6034 1493 Size: 961 1093 226 152 2109 1697 Fragments arranged by size: 2109 1697 1093 961 226 152 AseI AT'TA_AT Cuts at: 160 1032 4792 160 Size: 872 3760 1606 Fragments arranged by size: 3760 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 3095 3563 1870 Size: 73 1152 468 4545 Fragments arranged by size: 4545 1152 468 73 BamHI G'GATC_C Cuts at: 3568 3935 3568 Size: 367 5871 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2460 3559 5014 618 Size: 325 217 627 673 1099 1455 1842 Fragments arranged by size: 1842 1455 1099 673 627 325 217 BanII G_rGCy'C Cuts at: 1391 3557 1391 Size: 2166 4072 BbeI G_GCGC'C Cuts at: 2464 2464 Size: 6238 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3040 928 Size: 971 1141 4126 Fragments arranged by size: 4126 1141 971 BbvCI CC'TCA_GC Cuts at: 3192 3192 Size: 6238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4429 4463 1903 Size: 34 2492 34 3678 Fragments arranged by size: 3678 2492 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 2268 1548 Size: 720 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2118 4849 136 Size: 107 122 71 631 605 446 2731 1525 Fragments arranged by size: 2731 1525 631 605 446 122 107 71 BglII A'GATC_T Cuts at: 6233 6233 Size: 6238 BlpI GC'TnA_GC Cuts at: 3655 3655 Size: 6238 Bme1580I G_kGCm'C Cuts at: 1790 2998 3290 3320 4299 5455 1790 Size: 1208 292 30 979 1156 2573 Fragments arranged by size: 2573 1208 1156 979 292 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2406 3969 4927 Size: 416 53 83 399 24 989 1563 958 Cuts at: 4927 442 Size: 1753 Fragments arranged by size: 1753 1563 989 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3182 3182 Size: 6238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2729 3157 4898 1239 Size: 1490 428 1741 2579 Fragments arranged by size: 2579 1741 1490 428 Bpu10I CC'TnA_GC Cuts at: 2232 3192 2232 Size: 960 5278 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3454 4363 5228 5526 3454 Size: 909 865 298 4166 Fragments arranged by size: 4166 909 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2291 2337 2671 2751 4901 882 Size: 617 792 46 334 80 2150 2219 Fragments arranged by size: 2219 2150 792 617 334 80 46 BsaAI yAC'GTr Cuts at: 493 1204 4029 493 Size: 711 2825 2702 Fragments arranged by size: 2825 2702 711 BsaBI GATnn'nnATC Cuts at: 2159 6232 2159 Size: 4073 2165 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2461 3113 4045 4427 Size: 53 83 186 936 928 652 932 382 Cuts at: 4427 5062 275 Size: 635 1451 Fragments arranged by size: 1451 936 932 928 652 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2868 4671 5069 5343 5490 5901 2868 Size: 1803 398 274 147 411 3205 Fragments arranged by size: 3205 1803 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2918 2948 3116 3146 1178 Size: 30 165 30 1515 30 168 30 4270 Fragments arranged by size: 4270 1515 168 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2606 1186 Size: 1420 4818 BseYI C'CCAG_C Cuts at: 2282 2301 2464 2515 2885 5441 5937 2282 Size: 19 163 51 370 2556 496 2583 Fragments arranged by size: 2583 2556 496 370 163 51 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3198 1163 Size: 573 1462 4203 Fragments arranged by size: 4203 1462 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3688 4449 5477 1540 Size: 275 186 1687 761 1028 2301 Fragments arranged by size: 2301 1687 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2868 4299 4384 5455 2868 Size: 1431 85 1071 3651 Fragments arranged by size: 3651 1431 1071 85 BsmI GAATG_Cn' Cuts at: 3225 3749 3842 3225 Size: 524 93 5621 Fragments arranged by size: 5621 524 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2177 2177 Size: 6238 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2868 2998 3290 3320 3557 4299 4384 Size: 399 1078 130 292 30 237 742 85 Cuts at: 4384 5455 1391 Size: 1071 2174 Fragments arranged by size: 2174 1078 1071 742 399 292 237 130 85 30 BspEI T'CCGG_A Cuts at: 5901 5901 Size: 6238 BspHI T'CATG_A Cuts at: 2913 4127 5857 2913 Size: 1214 1730 3294 Fragments arranged by size: 3294 1730 1214 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2843 3593 844 Size: 1999 750 3489 Fragments arranged by size: 3489 1999 750 BsrBI CCG'CTC Cuts at: 1394 1823 4125 1394 Size: 429 2302 3507 Fragments arranged by size: 3507 2302 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2277 3330 3348 4733 4907 65 Size: 1183 1029 1053 18 1385 174 1396 Fragments arranged by size: 1396 1385 1183 1053 1029 174 18 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 2406 4882 5069 1700 Size: 110 311 285 2476 187 2869 Fragments arranged by size: 2869 2476 311 285 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6238 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 4298 5310 4298 Size: 1012 5226 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6238 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 3253 1184 Size: 2069 4169 BstXI CCAn_nnnn'nTGG Cuts at: 2447 2447 Size: 6238 BstYI r'GATC_y Cuts at: 1972 2154 3090 3568 3935 4320 4337 5789 5875 Size: 182 936 478 367 385 17 1452 86 Cuts at: 5875 5887 6233 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 936 478 385 367 346 182 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3023 3023 Size: 6238 BtgI C'CryG_G Cuts at: 513 1065 2004 2529 2661 513 Size: 552 939 525 132 4090 Fragments arranged by size: 4090 939 552 525 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5499 Fragments arranged by size: 5499 683 56 BtsI GCAGTG_nn' Cuts at: 2576 2714 3255 3754 4548 4568 2576 Size: 138 541 499 794 20 4246 Fragments arranged by size: 4246 794 541 499 138 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 3542 3542 Size: 6238 DraIII CAC_nnn'GTG Cuts at: 3324 3324 Size: 6238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5245 809 Size: 507 3929 1802 Fragments arranged by size: 3929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2658 3015 3685 Size: 54 1425 50 275 18 828 357 670 Cuts at: 3685 4574 6203 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 828 670 357 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2070 3155 3173 4168 1353 Size: 717 1085 18 995 3423 Fragments arranged by size: 3423 1085 995 717 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3023 4825 5199 5345 387 Size: 1064 138 1434 1802 374 146 1280 Fragments arranged by size: 1802 1434 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2729 2765 2887 3128 3157 4301 4898 Size: 393 1097 36 122 241 29 1144 597 Cuts at: 4898 5685 5953 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1144 1097 787 597 393 268 241 122 36 29 EcoO109I rG'GnC_Cy Cuts at: 2228 3340 2228 Size: 1112 5126 EcoRI G'AATT_C Cuts at: 3547 3925 3547 Size: 378 5860 EcoRV GAT'ATC Cuts at: 2485 2485 Size: 6238 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2914 2914 Size: 6238 FseI GG_CCGG'CC Cuts at: 2125 2125 Size: 6238 FspI TGC'GCA Cuts at: 4744 4744 Size: 6238 HaeII r_GCGC'y Cuts at: 1678 2464 2646 5385 1678 Size: 786 182 2739 2531 Fragments arranged by size: 2739 2531 786 182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2486 2518 2918 Size: 32 135 32 515 32 367 32 400 Cuts at: 2918 2950 3194 3226 4879 4911 4953 4985 1373 Size: 32 244 32 1653 32 42 32 2626 Fragments arranged by size: 2626 1653 515 400 367 244 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3582 3828 669 Size: 988 281 1644 246 3079 Fragments arranged by size: 3079 1644 988 281 246 HindIII A'AGCT_T Cuts at: 748 2198 748 Size: 1450 4788 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KasI G'GCGC_C Cuts at: 2460 2460 Size: 6238 KpnI G_GTAC'C Cuts at: 3563 3563 Size: 6238 MfeI C'AATT_G Cuts at: 3837 3837 Size: 6238 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2169 3402 5352 5536 1305 Size: 864 1233 1950 184 2007 Fragments arranged by size: 2007 1950 1233 864 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3017 10 Size: 54 1425 343 1185 3231 Fragments arranged by size: 3231 1425 1185 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4196 4555 4714 518 Size: 648 843 2187 359 159 2042 Fragments arranged by size: 2187 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2263 3654 4331 5479 5724 1606 Size: 657 1391 677 1148 245 2120 Fragments arranged by size: 2120 1391 1148 677 657 245 NaeI GCC'GGC Cuts at: 2123 2123 Size: 6238 NarI GG'CG_CC Cuts at: 2461 2461 Size: 6238 NcoI C'CATG_G Cuts at: 513 1065 2004 2529 513 Size: 552 939 525 4222 Fragments arranged by size: 4222 939 552 525 NdeI CA'TA_TG Cuts at: 387 387 Size: 6238 NgoMIV G'CCGG_C Cuts at: 2121 2121 Size: 6238 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 3010 3286 3596 5141 1132 Size: 97 1781 276 310 1545 2229 Fragments arranged by size: 2229 1781 1545 310 276 97 PasI CC'CwG_GG Cuts at: 1348 2225 3299 1348 Size: 877 1074 4287 Fragments arranged by size: 4287 1074 877 PciI A'CATG_T Cuts at: 3006 3282 5137 3006 Size: 276 1855 4107 Fragments arranged by size: 4107 1855 276 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6238 PfoI T'CCnGG_A Cuts at: 2203 2541 2727 2976 2203 Size: 338 186 249 5465 Fragments arranged by size: 5465 338 249 186 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3340 3340 Size: 6238 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6238 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PstI C_TGCA'G Cuts at: 830 3090 3590 830 Size: 2260 500 3478 Fragments arranged by size: 3478 2260 500 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 SalI G'TCGA_C Cuts at: 1936 3580 1936 Size: 1644 4594 SapI GCTCTTCn'nnn_ Cuts at: 2070 2070 Size: 6238 SbfI CC_TGCA'GG Cuts at: 3590 3590 Size: 6238 ScaI AGT'ACT Cuts at: 4486 6198 4486 Size: 1712 4526 SfcI C'TryA_G Cuts at: 826 1045 3086 3267 3586 4721 5402 5593 826 Size: 219 2041 181 319 1135 681 191 1471 Fragments arranged by size: 2041 1471 1135 681 319 219 191 181 SfoI GGC'GCC Cuts at: 2462 2462 Size: 6238 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6238 SmaI CCC'GGG Cuts at: 1872 3565 1872 Size: 1693 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3469 4342 5243 5505 820 Size: 197 926 1526 873 901 262 1553 Fragments arranged by size: 1553 1526 926 901 873 262 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6238 SphI G_CATG'C Cuts at: 1132 3596 1132 Size: 2464 3774 SspI AAT'ATT Cuts at: 5 52 4162 5 Size: 47 4110 2081 Fragments arranged by size: 4110 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6238 StyI C'CwwG_G Cuts at: 513 1065 2004 2529 3078 3677 513 Size: 552 939 525 549 599 3074 Fragments arranged by size: 3074 939 599 552 549 525 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4265 4282 4435 4620 5065 325 Size: 1255 2685 17 153 185 445 1498 Fragments arranged by size: 2685 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4484 6196 96 Size: 274 80 33 51 1443 2507 1712 138 Fragments arranged by size: 2507 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6198 6230 6198 Size: 32 6206 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6238 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2704 3124 2704 Size: 420 5818 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6238 XmaI C'CCGG_G Cuts at: 1870 3563 1870 Size: 1693 4545 XmnI GAAnn'nnTTC Cuts at: 4367 4367 Size: 6238 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI EcoNI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI