(Linear) (Six-base) MAPSORT of: KIEE4123.seq Check: 6270 from: 1 to: 1533 KIEE4123.orf With 182 enzymes: SgfI * July 3, 2012 18:37 .. AccI GT'mk_AC Cuts at: 0 826 1178 1533 Size: 826 352 355 Fragments arranged by size: 826 355 352 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 760 882 1123 1533 Size: 760 122 241 410 Fragments arranged by size: 760 410 241 122 AfeI AGC'GCT Cuts at: 0 639 1533 Size: 639 894 AflIII A'CryG_T Cuts at: 0 1001 1277 1533 Size: 1001 276 256 Fragments arranged by size: 1001 276 256 AlwNI CAG_nnn'CTG Cuts at: 0 226 1533 Size: 226 1307 ApoI r'AATT_y Cuts at: 0 449 1533 Size: 449 1084 AvaI C'yCGr_G Cuts at: 0 1090 1533 Size: 1090 443 BanI G'GyrC_C Cuts at: 0 455 1533 Size: 455 1078 BbeI G_GCGC'C Cuts at: 0 459 1533 Size: 459 1074 BbsI GAAGACnn'nnnn_ Cuts at: 0 1035 1533 Size: 1035 498 BbvCI CC'TCA_GC Cuts at: 0 1187 1533 Size: 1187 346 BclI T'GATC_A Cuts at: 0 263 1533 Size: 263 1270 BglI GCCn_nnn'nGGC Cuts at: 0 113 1533 Size: 113 1420 Bme1580I G_kGCm'C Cuts at: 0 993 1285 1315 1533 Size: 993 292 30 218 Fragments arranged by size: 993 292 218 30 BmrI ACTGGGnnnn_n' Cuts at: 0 401 1533 Size: 401 1132 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1177 1533 Size: 1177 356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 724 1152 1533 Size: 724 428 381 Fragments arranged by size: 724 428 381 Bpu10I CC'TnA_GC Cuts at: 0 227 1187 1533 Size: 227 960 346 Fragments arranged by size: 960 346 227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1449 1533 Size: 1449 84 BsaI GGTCTCn'nnnn_ Cuts at: 0 286 332 666 746 1533 Size: 286 46 334 80 787 Fragments arranged by size: 787 334 286 80 46 BsaBI GATnn'nnATC Cuts at: 0 154 1533 Size: 154 1379 BsaHI Gr'CG_yC Cuts at: 0 456 1108 1533 Size: 456 652 425 Fragments arranged by size: 652 456 425 BsaWI w'CCGG_w Cuts at: 0 863 1533 Size: 863 670 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 913 943 1111 1141 1533 Size: 913 30 168 30 392 Fragments arranged by size: 913 392 168 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 601 1533 Size: 601 932 BseYI C'CCAG_C Cuts at: 0 277 296 459 510 880 1533 Size: 277 19 163 51 370 653 Fragments arranged by size: 653 370 277 163 51 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1193 1533 Size: 1193 340 BsiHKAI G_wGCw'C Cuts at: 0 863 1533 Size: 863 670 BsmI GAATG_Cn' Cuts at: 0 1220 1533 Size: 1220 313 BsmBI CGTCTCn'nnnn_ Cuts at: 0 172 1533 Size: 172 1361 Bsp1286I G_dGCh'C Cuts at: 0 863 993 1285 1315 1533 Size: 863 130 292 30 218 Fragments arranged by size: 863 292 218 130 30 BspHI T'CATG_A Cuts at: 0 908 1533 Size: 908 625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 838 1533 Size: 838 695 BsrDI GCAATG_nn' Cuts at: 0 272 1325 1343 1533 Size: 272 1053 18 190 Fragments arranged by size: 1053 272 190 18 BsrFI r'CCGG_y Cuts at: 0 116 401 1533 Size: 116 285 1132 Fragments arranged by size: 1132 285 116 BstEII G'GTnAC_C Cuts at: 0 1248 1533 Size: 1248 285 BstXI CCAn_nnnn'nTGG Cuts at: 0 442 1533 Size: 442 1091 BstYI r'GATC_y Cuts at: 0 149 1085 1533 Size: 149 936 448 Fragments arranged by size: 936 448 149 Bsu36I CC'TnA_GG Cuts at: 0 1018 1533 Size: 1018 515 BtgI C'CryG_G Cuts at: 0 524 656 1533 Size: 524 132 877 Fragments arranged by size: 877 524 132 BtsI GCAGTG_nn' Cuts at: 0 571 709 1250 1533 Size: 571 138 541 283 Fragments arranged by size: 571 541 283 138 DraIII CAC_nnn'GTG Cuts at: 0 1319 1533 Size: 1319 214 EaeI y'GGCC_r Cuts at: 0 653 1010 1533 Size: 653 357 523 Fragments arranged by size: 653 523 357 EarI CTCTTCn'nnn_ Cuts at: 0 65 1150 1168 1533 Size: 65 1085 18 365 Fragments arranged by size: 1085 365 65 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1018 1533 Size: 1018 515 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 724 760 882 1123 1152 1533 Size: 724 36 122 241 29 381 Fragments arranged by size: 724 381 241 122 36 29 EcoO109I rG'GnC_Cy Cuts at: 0 223 1335 1533 Size: 223 1112 198 Fragments arranged by size: 1112 223 198 EcoRV GAT'ATC Cuts at: 0 480 1533 Size: 480 1053 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 909 1533 Size: 909 624 FseI GG_CCGG'CC Cuts at: 0 120 1533 Size: 120 1413 HaeII r_GCGC'y Cuts at: 0 459 641 1533 Size: 459 182 892 Fragments arranged by size: 892 459 182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 82 114 481 513 913 945 1189 1221 Size: 82 32 367 32 400 32 244 32 Cuts at: 1221 1533 Size: 312 Fragments arranged by size: 400 367 312 244 82 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 193 1533 Size: 193 1340 KasI G'GCGC_C Cuts at: 0 455 1533 Size: 455 1078 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 164 1397 1533 Size: 164 1233 136 Fragments arranged by size: 1233 164 136 MscI TGG'CCA Cuts at: 0 1012 1533 Size: 1012 521 MspA1I CmG'CkG Cuts at: 0 258 1533 Size: 258 1275 NaeI GCC'GGC Cuts at: 0 118 1533 Size: 118 1415 NarI GG'CG_CC Cuts at: 0 456 1533 Size: 456 1077 NcoI C'CATG_G Cuts at: 0 524 1533 Size: 524 1009 NgoMIV G'CCGG_C Cuts at: 0 116 1533 Size: 116 1417 NspI r_CATG'y Cuts at: 0 1005 1281 1533 Size: 1005 276 252 Fragments arranged by size: 1005 276 252 PasI CC'CwG_GG Cuts at: 0 220 1294 1533 Size: 220 1074 239 Fragments arranged by size: 1074 239 220 PciI A'CATG_T Cuts at: 0 1001 1277 1533 Size: 1001 276 256 Fragments arranged by size: 1001 276 256 PfoI T'CCnGG_A Cuts at: 0 198 536 722 971 1533 Size: 198 338 186 249 562 Fragments arranged by size: 562 338 249 198 186 PpuMI rG'GwC_Cy Cuts at: 0 1335 1533 Size: 1335 198 PstI C_TGCA'G Cuts at: 0 1085 1533 Size: 1085 448 SapI GCTCTTCn'nnn_ Cuts at: 0 65 1533 Size: 65 1468 SfcI C'TryA_G Cuts at: 0 1081 1262 1533 Size: 1081 181 271 Fragments arranged by size: 1081 271 181 SfoI GGC'GCC Cuts at: 0 457 1533 Size: 457 1076 SmlI C'TyrA_G Cuts at: 0 1464 1533 Size: 1464 69 StyI C'CwwG_G Cuts at: 0 524 1073 1533 Size: 524 549 460 Fragments arranged by size: 549 524 460 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 699 1119 1533 Size: 699 420 414 Fragments arranged by size: 699 420 414 Enzymes that do cut: AccI AcuI AfeI AflIII AlwNI ApoI AvaI BanI BbeI BbsI BbvCI BclI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRV FalI FseI HaeII Hin4I HindIII KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PpuMI PstI SapI SfcI SfoI SmlI StyI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BcgI BciVI BfrBI BglII BlpI BmgBI BmtI BsaAI BsiEI BsiWI BspEI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI FspI FspAI HincII HpaI KpnI MfeI MluI MslI NdeI NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI