################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AvrII BalI BbeI BbrPI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 386 GGTACC G^GTACC AcsI 732 GAATTT [GA]^AATT[CT] Aor51HI 356 AGCGCT AGC^GCT ApaI 707 GGGCCC GGGCC^C ApoI 732 GAATTT [GA]^AATT[CT] Asp718I 386 GGTACC G^GTACC AspHI 142 GTGCTC G[AT]GC[AT]^C AvaII 588 GGTCC G^G[AT]CC AviII 167 TGCGCA TGC^GCA BanI 386 GGTACC G^G[CT][GA]CC BbvCI 225 CCTCAGC CCTCAGC(-5/-2) BcgI 284 GCAAAATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 617 CCCGG CC^[GC]GG BfaI 509 CTAG C^TAG BlpI 336 GCTGAGC GC^T[ACGT]AGC Bpu1102I 336 GCTGAGC GC^T[ACGT]AGC BsaAI 643 TACGTG [CT]AC^GT[GA] BsaJI 153 CCTGGG C^C[ACGT][ACGT]GG BsiEI 419 CGGTCG CG[GA][CT]^CG BsiHKAI 142 GTGCTC G[AT]GC[AT]^C BsmI 698 GCATTC GAATGC(1/-1) BstXI 538 CCAGTCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 336 GCTGAGC GC^T[ACGT]AGC EaeI 203 TGGCCG [CT]^GGCC[GA] EarI 220 CTCTTC CTCTTC(1/4) Eco47III 356 AGCGCT AGC^GCT FspI 167 TGCGCA TGC^GCA HapII 618 CCGG C^CGG HgaI 840 GCGTC GACGC(5/10) HpaII 618 CCGG C^CGG HphI 910 GGTGA GGTGA(8/7) KpnI 386 GGTACC GGTAC^C Ksp632I 220 CTCTTC CTCTTC(1/4) MaeI 509 CTAG C^TAG MspI 618 CCGG C^CGG NciI 617 CCCGG CC^[GC]GG PleI 60 GACTC GAGTC(4/5) PstI 883 CTGCAG CTGCA^G SfcI 883 CTGCAG C^T[GA][CT]AG SphI 164 GCATGC GCATG^C StuI 324 AGGCCT AGG^CCT TfiI 505 GATTC G^A[AT]TC TspRI 662 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 135 CGCG CG^CG 543 CGCG AlwNI 76 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 853 CAGAACCTG AocI 895 CCTGAGG CC^T[ACGT]AGG 925 CCTGAGG BbsI 19 GTCTTC GAAGAC(2/6) 670 GTCTTC BpuAI 19 GTCTTC GAAGAC(2/6) 670 GTCTTC BseRI 676 CTCCTC GAGGAG(10/8) 55 GAGGAG BsmAI 58 GAGAC GTCTC(1/5) 181 GTCTC BsrI 16 CCAGT ACTGG(1/-1) 538 CCAGT BstUI 135 CGCG CG^CG 543 CGCG Bsu36I 895 CCTGAGG CC^T[ACGT]AGG 925 CCTGAGG Cac8I 164 GCATGC GC[ACGT]^[ACGT]GC 686 GCCTGC Eco81I 895 CCTGAGG CC^T[ACGT]AGG 925 CCTGAGG HaeII 340 AGCGCC [GA]GCGC^[CT] 356 AGCGCT HinfI 60 GACTC G^A[ACGT]TC 505 GATTC MaeIII 482 GTGAC ^GT[ACGT]AC 646 GTGAC MvnI 135 CGCG CG^CG 543 CGCG NspI 164 GCATGC [GA]CATG^[CT] 770 GCATGT Tsp45I 482 GTGAC ^GT[GC]AC 646 GTGAC Tsp509I 714 AATT ^AATT 733 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AfaI AluI AlwI BamHI BanII BbvI BmyI BpmI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HhaI HinP1I ItaI MaeII MboI MboII MflI MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI XhoII