################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII KasI KpnI KspI MamI MfeI MflI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 537 GTCTAC GT^[AC][GT]AC AciI 415 CCGC CCGC(-3/-1) AclI 789 AACGTT AA^CGTT AflIII 277 ACATGT A^C[GA][CT]GT Alw44I 1431 GTGCAC G^TGCAC ApaLI 1431 GTGCAC G^TGCAC BalI 2 TGGCCA TGG^CCA BbvI 121 GCTGC GCAGC(8/12) BciVI 1347 GTATCC GTATCC(6/5) BsgI 122 CTGCAC GTGCAG(16/14) BsmFI 1487 GGGAC GGGAC(10/14) Bsp1407I 720 TGTACA T^GTACA BspLU11I 277 ACATGT A^CATGT BsrDI 1058 GCAATG GCAATG(2/0) BsrGI 720 TGTACA T^GTACA BssSI 1176 CACGAG CACGAG(-5/-1) BstXI 638 CCAGTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 475 GATC ^GATC DraI 582 TTTAAA TTT^AAA DraII 1487 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 2 TGGCCA [CT]^GGCC[GA] EcoO109I 1487 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 5 CCAAGG C^C[AT][AT]GG EcoT22I 290 ATGCAT ATGCA^T Fnu4HI 121 GCTGC GC^[ACGT]GC HincII 757 GTTAAC GT[CT]^[GA]AC HindII 757 GTTAAC GT[CT]^[GA]AC HpaI 757 GTTAAC GTT^AAC ItaI 121 GCTGC GC^[ACGT]GC MboI 475 GATC ^GATC MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MspA1I 250 CAGCTG C[AC]G^C[GT]G NdeII 475 GATC ^GATC NsiI 290 ATGCAT ATGCA^T PpuMI 1487 GGGACCC [GA]G^G[AT]CC[CT] Psp1406I 789 AACGTT AA^CGTT PvuII 250 CAGCTG CAG^CTG Sau3AI 475 GATC ^GATC SpeI 693 ACTAGT A^CTAGT SspBI 720 TGTACA T^GTACA StuI 1204 AGGCCT AGG^CCT StyI 5 CCAAGG C^C[AT][AT]GG TseI 121 GCTGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 721 GTAC GT^AC 1161 GTAC Asp700I 457 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1367 GAAGACTTTC AspHI 1220 GAGCAC G[AT]GC[AT]^C 1431 GTGCAC BanII 1456 GAGCCC G[GA]GC[CT]^C 1565 GAGCCC BpmI 387 CTCCAG CTGGAG(16/14) 1570 CTGGAG BsaJI 5 CCAAGG C^C[ACGT][ACGT]GG 1568 CCCTGG BsiHKAI 1220 GAGCAC G[AT]GC[AT]^C 1431 GTGCAC BsiYI 445 CCAGGACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1404 CCCAGCAGTGG BslI 445 CCAGGACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1404 CCCAGCAGTGG BsmBI 1246 GAGACG CGTCTC(1/5) 1548 GAGACG BstBI 667 TTCGAA TT^CGAA 1470 TTCGAA BstF5I 243 CATCC GGATG(2/0) 624 GGATG Cac8I 1399 GCAAGC GC[ACGT]^[ACGT]GC 1438 GCCAGC EcoRI 664 GAATTC G^AATTC 1515 GAATTC EcoRV 238 GATATC GAT^ATC 1327 GATATC FokI 243 CATCC GGATG(9/13) 624 GGATG HaeIII 3 GGCC GG^CC 1205 GGCC NspI 277 ACATGT [GA]CATG^[CT] 701 ACATGC NspV 667 TTCGAA TT^CGAA 1470 TTCGAA PleI 1063 GACTC GAGTC(4/5) 1087 GAGTC RsaI 721 GTAC GT^AC 1161 GTAC SfaNI 14 GATGC GCATC(5/9) 997 GATGC SfcI 247 CTACAG C^T[GA][CT]AG 1644 CTGTAG SfuI 667 TTCGAA TT^CGAA 1470 TTCGAA SmlI 494 CTCAAG C^T[CT][GA]AG 1677 CTTGAG Tsp45I 1198 GTCAC ^GT[GC]AC 1305 GTCAC XmnI 457 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1367 GAAGACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AcsI AluI ApoI AvaII BbsI BfaI BmyI BpuAI BsmAI Bsp1286I BspMI BsrI BssKI BstNI Cfr13I DdeI EarI EcoRII HinfI HphI Ksp632I MaeI MaeII MaeIII MboII MnlI MseI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I