(Circular) (Six-base) MAPSORT of: pFN21AE9622.seq Check: 5417 from: 1 to: 6388 pFN21AE9622 6388 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3595 3595 Size: 6388 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4198 278 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2543 3731 1937 Size: 606 1188 4594 Fragments arranged by size: 4594 1188 606 Acc65I G'GTAC_C Cuts at: 3709 3709 Size: 6388 AclI AA'CG_TT Cuts at: 1563 2795 4515 4888 1563 Size: 1232 1720 373 3063 Fragments arranged by size: 3063 1720 1232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3117 3347 4451 5835 6103 1632 Size: 1485 230 1104 1384 268 1917 Fragments arranged by size: 1917 1485 1384 1104 268 230 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6191 AflIII A'CryG_T Cuts at: 1203 2282 5287 6264 1203 Size: 1079 3005 977 1327 Fragments arranged by size: 3005 1327 1079 977 AgeI A'CCGG_T Cuts at: 5219 5219 Size: 6388 AhdI GACnn_n'nnGTC Cuts at: 5117 5117 Size: 6388 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6388 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6388 AlwNI CAG_nnn'CTG Cuts at: 4131 5703 4131 Size: 1572 4816 ApaLI G'TGCA_C Cuts at: 3436 4445 5601 3436 Size: 1009 1156 4223 Fragments arranged by size: 4223 1156 1009 ApoI r'AATT_y Cuts at: 1493 2398 2424 2669 3520 3626 3697 3923 4075 Size: 905 26 245 851 106 71 226 152 Cuts at: 4075 6184 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 905 851 245 226 152 106 71 26 AseI AT'TA_AT Cuts at: 160 1032 4942 160 Size: 872 3910 1606 Fragments arranged by size: 3910 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 AvaI C'yCGr_G Cuts at: 1870 1943 3713 1870 Size: 73 1770 4545 Fragments arranged by size: 4545 1770 73 BamHI G'GATC_C Cuts at: 3718 4085 3718 Size: 367 6021 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3709 5164 618 Size: 325 217 627 1922 1455 1842 Fragments arranged by size: 1922 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3465 3574 3707 1391 Size: 2074 109 133 4072 Fragments arranged by size: 4072 2074 133 109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 2820 3379 928 Size: 971 159 762 559 3937 Fragments arranged by size: 3937 971 762 559 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4579 4613 1903 Size: 34 2642 34 3678 Fragments arranged by size: 3678 2642 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3363 4280 5490 3363 Size: 917 1210 4261 Fragments arranged by size: 4261 1210 917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6388 BfrBI ATG'CAT Cuts at: 2297 2297 Size: 6388 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4999 136 Size: 107 122 71 631 605 3327 1525 Fragments arranged by size: 3327 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6383 6383 Size: 6388 BlpI GC'TnA_GC Cuts at: 3805 3805 Size: 6388 Bme1580I G_kGCm'C Cuts at: 1790 3440 4449 5605 1790 Size: 1650 1009 1156 2573 Fragments arranged by size: 2573 1650 1156 1009 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6388 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2519 4119 5077 Size: 416 53 83 399 24 1102 1600 958 Cuts at: 5077 442 Size: 1753 Fragments arranged by size: 1753 1600 1102 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6388 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2377 3596 5048 1239 Size: 1138 1219 1452 2579 Fragments arranged by size: 2579 1452 1219 1138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2484 3703 4513 5378 5676 2484 Size: 1219 810 865 298 3196 Fragments arranged by size: 3196 1219 865 810 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5051 882 Size: 617 3552 2219 Fragments arranged by size: 3552 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4179 493 Size: 711 2975 2702 Fragments arranged by size: 2975 2702 711 BsaBI GATnn'nnATC Cuts at: 6382 6382 Size: 6388 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4195 4577 5212 275 Size: 53 83 186 936 2662 382 635 1451 Fragments arranged by size: 2662 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4821 5219 5493 5640 6051 4821 Size: 398 274 147 411 5158 Fragments arranged by size: 5158 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3262 3292 1178 Size: 30 165 30 1859 30 4274 Fragments arranged by size: 4274 1859 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6388 BseYI C'CCAG_C Cuts at: 3409 5591 6087 3409 Size: 2182 496 3710 Fragments arranged by size: 3710 2182 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2112 1163 Size: 573 376 5439 Fragments arranged by size: 5439 573 376 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3838 4599 5627 1540 Size: 275 186 1837 761 1028 2301 Fragments arranged by size: 2301 1837 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3229 3440 4449 4534 5605 3229 Size: 211 1009 85 1071 4012 Fragments arranged by size: 4012 1071 1009 211 85 BsmI GAATG_Cn' Cuts at: 3899 3992 3899 Size: 93 6295 BsmBI CGTCTCn'nnnn_ Cuts at: 3245 3547 3245 Size: 302 6086 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3229 3440 3465 3574 3707 4449 4534 Size: 399 1439 211 25 109 133 742 85 Cuts at: 4534 5605 1391 Size: 1071 2174 Fragments arranged by size: 2174 1439 1071 742 399 211 133 109 85 25 BspEI T'CCGG_A Cuts at: 6051 6051 Size: 6388 BspHI T'CATG_A Cuts at: 4277 6007 4277 Size: 1730 4658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2103 2344 3595 3743 844 Size: 1259 241 1251 148 3489 Fragments arranged by size: 3489 1259 1251 241 148 BsrBI CCG'CTC Cuts at: 1394 1823 4275 1394 Size: 429 2452 3507 Fragments arranged by size: 3507 2452 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3070 4883 5057 65 Size: 1183 1822 1813 174 1396 Fragments arranged by size: 1822 1813 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5032 5219 1700 Size: 110 3222 187 2869 Fragments arranged by size: 3222 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2725 96 Size: 2629 3759 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6388 BssSI C'ACGA_G Cuts at: 3181 4448 5460 3181 Size: 1267 1012 4109 Fragments arranged by size: 4109 1267 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6388 BstBI TT'CG_AA Cuts at: 2673 3476 5192 2673 Size: 803 1716 3869 Fragments arranged by size: 3869 1716 803 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6388 BstXI CCAn_nnnn'nTGG Cuts at: 2650 2650 Size: 6388 BstYI r'GATC_y Cuts at: 1972 3718 4085 4470 4487 5939 6025 6037 6383 Size: 1746 367 385 17 1452 86 12 346 Cuts at: 6383 1972 Size: 1977 Fragments arranged by size: 1977 1746 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4897 Fragments arranged by size: 4897 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5649 Fragments arranged by size: 5649 683 56 BtsI GCAGTG_nn' Cuts at: 2159 3420 3904 4698 4718 2159 Size: 1261 484 794 20 3829 Fragments arranged by size: 3829 1261 794 484 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6388 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6353 DraI TTT'AAA Cuts at: 2589 3692 2589 Size: 1103 5285 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5395 809 Size: 507 4079 1802 Fragments arranged by size: 4079 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3835 4724 Size: 54 1425 50 275 18 177 1828 889 Cuts at: 4724 6353 8 Size: 1629 43 Fragments arranged by size: 1828 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3835 1537 Size: 275 2023 4090 Fragments arranged by size: 4090 2023 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2877 3018 3481 4318 1353 Size: 1524 141 463 837 3423 Fragments arranged by size: 3423 1524 837 463 141 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4975 5349 5495 387 Size: 1064 138 3386 374 146 1280 Fragments arranged by size: 3386 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2377 3117 3347 3596 4451 5048 5835 Size: 393 745 740 230 249 855 597 787 Cuts at: 5835 6103 1239 Size: 268 1524 Fragments arranged by size: 1524 855 787 745 740 597 393 268 249 230 EcoO109I rG'GnC_Cy Cuts at: 3493 3493 Size: 6388 EcoRI G'AATT_C Cuts at: 2669 3520 3697 4075 2669 Size: 851 177 378 4982 Fragments arranged by size: 4982 851 378 177 EcoRV GAT'ATC Cuts at: 2245 3334 2245 Size: 1089 5299 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2063 2327 2063 Size: 264 6124 FspI TGC'GCA Cuts at: 4894 4894 Size: 6388 HaeII r_GCGC'y Cuts at: 1678 5535 1678 Size: 3857 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 5029 5061 5103 Size: 32 135 32 803 32 2622 32 42 Cuts at: 5103 5135 1373 Size: 32 2626 Fragments arranged by size: 2626 2622 803 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2764 3732 3978 669 Size: 988 281 826 968 246 3079 Fragments arranged by size: 3079 988 968 826 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6388 HpaI GTT'AAC Cuts at: 2764 3978 2764 Size: 1214 5174 KpnI G_GTAC'C Cuts at: 3713 3713 Size: 6388 MfeI C'AATT_G Cuts at: 3987 3987 Size: 6388 MluI A'CGCG_T Cuts at: 6264 6264 Size: 6388 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2662 3073 5502 5686 1305 Size: 1357 411 2429 184 2007 Fragments arranged by size: 2429 2007 1357 411 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4389 Fragments arranged by size: 4389 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 4346 4705 4864 518 Size: 648 3180 359 159 2042 Fragments arranged by size: 3180 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2257 3804 4481 5629 5874 1606 Size: 651 1547 677 1148 245 2120 Fragments arranged by size: 2120 1547 1148 677 651 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4897 Fragments arranged by size: 4897 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6388 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6388 NotI GC'GGCC_GC Cuts at: 3835 3835 Size: 6388 NsiI A_TGCA'T Cuts at: 2299 2299 Size: 6388 NspI r_CATG'y Cuts at: 1132 1229 2286 2710 3746 5291 1132 Size: 97 1057 424 1036 1545 2229 Fragments arranged by size: 2229 1545 1057 1036 424 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6388 PciI A'CATG_T Cuts at: 2282 5287 2282 Size: 3005 3383 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6388 PfoI T'CCnGG_A Cuts at: 2449 2449 Size: 6388 PmeI GTTT'AAAC Cuts at: 3692 3692 Size: 6388 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4457 4489 5970 6002 4457 Size: 32 1481 32 4843 Fragments arranged by size: 4843 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3493 3493 Size: 6388 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6388 PsiI TTA'TAA Cuts at: 3958 3958 Size: 6388 PstI C_TGCA'G Cuts at: 830 3740 830 Size: 2910 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6388 PvuII CAG'CTG Cuts at: 2257 2257 Size: 6388 SalI G'TCGA_C Cuts at: 1936 3730 1936 Size: 1794 4594 SanDI GG'GwC_CC Cuts at: 3493 3493 Size: 6388 SbfI CC_TGCA'GG Cuts at: 3740 3740 Size: 6388 ScaI AGT'ACT Cuts at: 4636 6348 4636 Size: 1712 4676 SfcI C'TryA_G Cuts at: 826 1045 2252 3649 3736 4871 5552 5743 826 Size: 219 1207 1397 87 1135 681 191 1471 Fragments arranged by size: 1471 1397 1207 1135 681 219 191 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6388 SmaI CCC'GGG Cuts at: 1872 3715 1872 Size: 1843 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2499 3682 4492 5393 5655 820 Size: 197 926 556 1183 810 901 262 1553 Fragments arranged by size: 1553 1183 926 901 810 556 262 197 SnaBI TAC'GTA Cuts at: 493 4179 493 Size: 3686 2702 SpeI A'CTAG_T Cuts at: 152 2698 152 Size: 2546 3842 SphI G_CATG'C Cuts at: 1132 3746 1132 Size: 2614 3774 SspI AAT'ATT Cuts at: 5 52 4312 5 Size: 47 4260 2081 Fragments arranged by size: 4260 2081 47 StuI AGG'CCT Cuts at: 1517 3211 1517 Size: 1694 4694 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 3827 513 Size: 552 939 6 1817 3074 Fragments arranged by size: 3074 1817 939 552 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4415 4432 4585 4770 5215 325 Size: 1255 2835 17 153 185 445 1498 Fragments arranged by size: 2835 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2725 3165 4634 Size: 274 80 33 51 1443 748 440 1469 Cuts at: 4634 6346 96 Size: 1712 138 Fragments arranged by size: 1712 1469 1443 748 440 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6348 6380 6348 Size: 32 6356 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6388 XbaI T'CTAG_A Cuts at: 3724 3724 Size: 6388 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6388 XmaI C'CCGG_G Cuts at: 1870 3713 1870 Size: 1843 4545 XmnI GAAnn'nnTTC Cuts at: 2466 3376 4517 2466 Size: 910 1141 4337 Fragments arranged by size: 4337 1141 910 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4196 276 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI