(Linear) (Six-base) MAPSORT of: KIEE9622.seq Check: 3328 from: 1 to: 1683 KIEE9622.orf With 182 enzymes: SgfI * July 3, 2012 19:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1590 1683 Size: 1590 93 AccI GT'mk_AC Cuts at: 0 538 1683 Size: 538 1145 AclI AA'CG_TT Cuts at: 0 790 1683 Size: 790 893 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1112 1342 1683 Size: 1112 230 341 Fragments arranged by size: 1112 341 230 AflIII A'CryG_T Cuts at: 0 277 1683 Size: 277 1406 ApaLI G'TGCA_C Cuts at: 0 1431 1683 Size: 1431 252 ApoI r'AATT_y Cuts at: 0 393 419 664 1515 1621 1683 Size: 393 26 245 851 106 62 Fragments arranged by size: 851 393 245 106 62 26 BanII G_rGCy'C Cuts at: 0 1460 1569 1683 Size: 1460 109 114 Fragments arranged by size: 1460 114 109 BbsI GAAGACnn'nnnn_ Cuts at: 0 53 815 1374 1683 Size: 53 762 559 309 Fragments arranged by size: 762 559 309 53 BciVI GTATCCnnnnn_n' Cuts at: 0 1358 1683 Size: 1358 325 BfrBI ATG'CAT Cuts at: 0 292 1683 Size: 292 1391 Bme1580I G_kGCm'C Cuts at: 0 1435 1683 Size: 1435 248 BmrI ACTGGGnnnn_n' Cuts at: 0 514 1683 Size: 514 1169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 372 1591 1683 Size: 372 1219 92 Fragments arranged by size: 1219 372 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 479 1683 Size: 479 1204 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1257 1287 1683 Size: 1257 30 396 Fragments arranged by size: 1257 396 30 BseYI C'CCAG_C Cuts at: 0 1404 1683 Size: 1404 279 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 107 1683 Size: 107 1576 BsiHKAI G_wGCw'C Cuts at: 0 1224 1435 1683 Size: 1224 211 248 Fragments arranged by size: 1224 248 211 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1240 1542 1683 Size: 1240 302 141 Fragments arranged by size: 1240 302 141 Bsp1286I G_dGCh'C Cuts at: 0 1224 1435 1460 1569 1683 Size: 1224 211 25 109 114 Fragments arranged by size: 1224 211 114 109 25 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 98 339 1590 1683 Size: 98 241 1251 93 Fragments arranged by size: 1251 241 98 93 BsrDI GCAATG_nn' Cuts at: 0 1065 1683 Size: 1065 618 BsrGI T'GTAC_A Cuts at: 0 720 1683 Size: 720 963 BssSI C'ACGA_G Cuts at: 0 1176 1683 Size: 1176 507 BstBI TT'CG_AA Cuts at: 0 668 1471 1683 Size: 668 803 212 Fragments arranged by size: 803 668 212 BstXI CCAn_nnnn'nTGG Cuts at: 0 645 1683 Size: 645 1038 BtsI GCAGTG_nn' Cuts at: 0 154 1415 1683 Size: 154 1261 268 Fragments arranged by size: 1261 268 154 DraI TTT'AAA Cuts at: 0 584 1683 Size: 584 1099 EaeI y'GGCC_r Cuts at: 0 2 1683 Size: 2 1681 EarI CTCTTCn'nnn_ Cuts at: 0 872 1013 1476 1683 Size: 872 141 463 207 Fragments arranged by size: 872 463 207 141 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 372 1112 1342 1591 1683 Size: 372 740 230 249 92 Fragments arranged by size: 740 372 249 230 92 EcoO109I rG'GnC_Cy Cuts at: 0 1488 1683 Size: 1488 195 EcoRI G'AATT_C Cuts at: 0 664 1515 1683 Size: 664 851 168 Fragments arranged by size: 851 664 168 EcoRV GAT'ATC Cuts at: 0 240 1329 1683 Size: 240 1089 354 Fragments arranged by size: 1089 354 240 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 58 322 1683 Size: 58 264 1361 Fragments arranged by size: 1361 264 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 370 402 1683 Size: 370 32 1281 Fragments arranged by size: 1281 370 32 HincII GTy'rAC Cuts at: 0 759 1683 Size: 759 924 HpaI GTT'AAC Cuts at: 0 759 1683 Size: 759 924 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 657 1068 1683 Size: 657 411 615 Fragments arranged by size: 657 615 411 MscI TGG'CCA Cuts at: 0 4 1683 Size: 4 1679 MspA1I CmG'CkG Cuts at: 0 252 1683 Size: 252 1431 NsiI A_TGCA'T Cuts at: 0 294 1683 Size: 294 1389 NspI r_CATG'y Cuts at: 0 281 705 1683 Size: 281 424 978 Fragments arranged by size: 978 424 281 PciI A'CATG_T Cuts at: 0 277 1683 Size: 277 1406 PfoI T'CCnGG_A Cuts at: 0 444 1683 Size: 444 1239 PpuMI rG'GwC_Cy Cuts at: 0 1488 1683 Size: 1488 195 PvuII CAG'CTG Cuts at: 0 252 1683 Size: 252 1431 SanDI GG'GwC_CC Cuts at: 0 1488 1683 Size: 1488 195 SfcI C'TryA_G Cuts at: 0 247 1644 1683 Size: 247 1397 39 Fragments arranged by size: 1397 247 39 SmlI C'TyrA_G Cuts at: 0 494 1677 1683 Size: 494 1183 6 Fragments arranged by size: 1183 494 6 SpeI A'CTAG_T Cuts at: 0 693 1683 Size: 693 990 StuI AGG'CCT Cuts at: 0 1206 1683 Size: 1206 477 StyI C'CwwG_G Cuts at: 0 5 1683 Size: 5 1678 TatI w'GTAC_w Cuts at: 0 720 1160 1683 Size: 720 440 523 Fragments arranged by size: 720 523 440 XmnI GAAnn'nnTTC Cuts at: 0 461 1371 1683 Size: 461 910 312 Fragments arranged by size: 910 461 312 Enzymes that do cut: AarI AccI AclI AcuI AflIII ApaLI ApoI BanII BbsI BciVI BfrBI Bme1580I BmrI BpmI BpuEI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrGI BssSI BstBI BstXI BtsI DraI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII HpaI MmeI MscI MspA1I NsiI NspI PciI PfoI PpuMI PvuII SanDI SfcI SmlI SpeI StuI StyI TatI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI BssHII BstAPI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI FseI FspI FspAI HaeII HindIII KasI KpnI MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI