################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspI AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MaeII MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AhdI 955 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 749 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 955 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 10 GAGCAC G[AT]GC[AT]^C AviII 563 TGCGCA TGC^GCA BanI 858 GGTGCC G^G[CT][GA]CC BsiHKAI 10 GAGCAC G[AT]GC[AT]^C BsrFI 189 GCCGGC [GA]^CCGG[CT] BssHII 17 GCGCGC G^CGCGC Cfr10I 189 GCCGGC [GA]^CCGG[CT] Eam1105I 955 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 573 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 807 ATGCAT ATGCA^T FspI 563 TGCGCA TGC^GCA HgaI 459 GCGTC GACGC(5/10) NaeI 189 GCCGGC GCC^GGC NgoMIV 189 GCCGGC G^CCGGC NsiI 807 ATGCAT ATGCA^T NspI 809 GCATGT [GA]CATG^[CT] PflMI 748 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 218 AGGACCT [GA]G^G[AT]CC[CT] SbfI 213 CCTGCAGG CCTGCA^GG Sse8387I 213 CCTGCAGG CCTGCA^GG Tsp45I 953 GTGAC ^GT[GC]AC TspRI 13 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 748 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AlwI 648 GATCC GGATC(4/5) 751 GGATC Asp700I 507 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 663 GAACCCCTTC BalI 386 TGGCCA TGG^CCA 437 TGGCCA BbsI 285 GAAGAC GAAGAC(2/6) 511 GTCTTC BbvCI 718 CCTCAGC CCTCAGC(-5/-2) 277 GCTGAGG BglI 329 GCCGAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 761 GCCCTGGAGGC BpuAI 285 GAAGAC GAAGAC(2/6) 511 GTCTTC BseRI 280 GAGGAG GAGGAG(10/8) 496 GAGGAG BsgI 38 CTGCAC GTGCAG(16/14) 112 CTGCAC BsmBI 975 CGTCTC CGTCTC(1/5) 344 GAGACG BsmFI 65 GGGAC GGGAC(10/14) 414 GGGAC BspMI 110 ACCTGC ACCTGC(4/8) 212 ACCTGC BsrI 1019 ACTGG ACTGG(1/-1) 743 CCAGT BstF5I 795 CATCC GGATG(2/0) 897 CATCC BstXI 428 CCATGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 825 CCACCCCCATGG BstYI 647 AGATCC [GA]^GATC[CT] 751 GGATCT DpnII 648 GATC ^GATC 752 GATC FokI 795 CATCC GGATG(9/13) 897 CATCC HinfI 416 GACTC G^A[ACGT]TC 424 GACTC MaeIII 464 GTAAC ^GT[ACGT]AC 953 GTGAC MboI 648 GATC ^GATC 752 GATC MflI 647 AGATCC [GA]^GATC[CT] 751 GGATCT MluNI 386 TGGCCA TGG^CCA 437 TGGCCA MscI 386 TGGCCA TGG^CCA 437 TGGCCA NdeII 648 GATC ^GATC 752 GATC PleI 416 GACTC GAGTC(4/5) 424 GACTC PstI 214 CTGCAG CTGCA^G 724 CTGCAG Sau3AI 648 GATC ^GATC 752 GATC SfcI 214 CTGCAG C^T[GA][CT]AG 724 CTGCAG XhoII 647 AGATCC [GA]^GATC[CT] 751 GGATCT XmnI 507 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 663 GAACCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AfaI AluI ApaI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI BsoBI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI KspI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NlaIII NlaIV PvuII RsaI SacII Sau96I ScrFI SfaNI SmaI StyI TseI XmaCI XmaI