(Circular) (Six-base) MAPSORT of: pFN21AB7681.seq Check: 5141 from: 1 to: 5734 pFN21AB7681 5734 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2124 2124 Size: 5734 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 3055 3055 Size: 5734 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2467 3797 5181 5449 1632 Size: 835 1330 1384 268 1917 Fragments arranged by size: 1917 1384 1330 835 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 4633 5610 1203 Size: 3430 977 1327 Fragments arranged by size: 3430 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 2965 4463 2965 Size: 1498 4236 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5734 AlwNI CAG_nnn'CTG Cuts at: 2759 3477 5049 2759 Size: 718 1572 3444 Fragments arranged by size: 3444 1572 718 ApaI G_GGCC'C Cuts at: 2087 2172 2235 2273 2345 2358 2087 Size: 85 63 38 72 13 5463 Fragments arranged by size: 5463 85 72 63 38 13 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 3043 3269 3421 5530 1493 Size: 1550 226 152 2109 1697 Fragments arranged by size: 2109 1697 1550 226 152 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2065 2228 2416 2550 2589 3059 Size: 73 69 53 163 188 134 39 470 Cuts at: 3059 1870 Size: 4545 Fragments arranged by size: 4545 470 188 163 134 73 69 53 39 BamHI G'GATC_C Cuts at: 3064 3431 3064 Size: 367 5367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2863 3055 4510 618 Size: 325 217 627 1076 192 1455 1842 Fragments arranged by size: 1842 1455 1076 627 325 217 192 BanII G_rGCy'C Cuts at: 1391 2087 2172 2181 2235 2273 2345 2358 2768 Size: 696 85 9 54 38 72 13 410 Cuts at: 2768 3053 1391 Size: 285 4072 Fragments arranged by size: 4072 696 410 285 85 72 54 38 13 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2297 2509 928 Size: 971 398 212 4153 Fragments arranged by size: 4153 971 398 212 BbvCI CC'TCA_GC Cuts at: 2283 2724 2283 Size: 441 5293 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3925 3959 1903 Size: 34 1988 34 3678 Fragments arranged by size: 3678 1988 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3626 4836 3626 Size: 1210 4524 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BfrBI ATG'CAT Cuts at: 2814 2814 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2340 2772 4345 Size: 107 122 71 631 605 668 432 1573 Cuts at: 4345 136 Size: 1525 Fragments arranged by size: 1573 1525 668 631 605 432 122 107 71 BglII A'GATC_T Cuts at: 5729 5729 Size: 5734 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 2087 2172 2235 2273 2345 2358 3795 4951 Size: 297 85 63 38 72 13 1437 1156 Cuts at: 4951 1790 Size: 2573 Fragments arranged by size: 2573 1437 1156 297 85 72 63 38 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5734 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3465 4423 442 Size: 416 53 83 399 24 2048 958 1753 Fragments arranged by size: 2048 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2311 2965 2311 Size: 654 5080 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 2519 2528 2775 2790 4394 1239 Size: 956 324 9 247 15 1604 2579 Fragments arranged by size: 2579 1604 956 324 247 15 9 Bpu10I CC'TnA_GC Cuts at: 2283 2724 2987 2283 Size: 441 263 5030 Fragments arranged by size: 5030 441 263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3859 4724 5022 3859 Size: 865 298 4571 Fragments arranged by size: 4571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4397 882 Size: 617 2898 2219 Fragments arranged by size: 2898 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3525 493 Size: 711 2321 2702 Fragments arranged by size: 2702 2321 711 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3541 3923 4558 275 Size: 53 83 186 936 2008 382 635 1451 Fragments arranged by size: 2008 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4167 4565 4839 4986 5397 4167 Size: 398 274 147 411 4504 Fragments arranged by size: 4504 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5509 Fragments arranged by size: 5509 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 2516 1186 Size: 1114 216 4404 Fragments arranged by size: 4404 1114 216 BseYI C'CCAG_C Cuts at: 2316 4937 5433 2316 Size: 2621 496 2617 Fragments arranged by size: 2621 2617 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2028 2102 1163 Size: 573 292 74 4795 Fragments arranged by size: 4795 573 292 74 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3184 3945 4973 1540 Size: 275 186 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2019 3795 3880 4951 2019 Size: 1776 85 1071 2802 Fragments arranged by size: 2802 1776 1071 85 BsmI GAATG_Cn' Cuts at: 3245 3338 3245 Size: 93 5641 BsmBI CGTCTCn'nnnn_ Cuts at: 2343 2986 2343 Size: 643 5091 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2087 2172 2181 2235 2273 2345 Size: 399 229 68 85 9 54 38 72 Cuts at: 2345 2358 2768 3053 3795 3880 4951 1391 Size: 13 410 285 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 410 399 285 229 85 85 72 68 54 38 13 9 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 3623 5353 3623 Size: 1730 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2124 2226 3089 844 Size: 1280 102 863 3489 Fragments arranged by size: 3489 1280 863 102 BsrBI CCG'CTC Cuts at: 1394 1823 3621 1394 Size: 429 1798 3507 Fragments arranged by size: 3507 1798 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4229 4403 65 Size: 1183 2981 174 1396 Fragments arranged by size: 2981 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2194 4378 4565 1700 Size: 110 384 2184 187 2869 Fragments arranged by size: 2869 2184 384 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 2022 1925 Size: 97 5637 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5734 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5734 BstXI CCAn_nnnn'nTGG Cuts at: 2440 2837 2440 Size: 397 5337 BstYI r'GATC_y Cuts at: 1972 2652 2756 3064 3431 3816 3833 5285 5371 Size: 680 104 308 367 385 17 1452 86 Cuts at: 5371 5383 5729 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 680 385 367 346 308 104 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2134 2141 2274 2433 2836 2905 Size: 552 939 130 7 133 159 403 69 Cuts at: 2905 3030 513 Size: 125 3217 Fragments arranged by size: 3217 939 552 403 159 133 130 125 69 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4995 Fragments arranged by size: 4995 683 56 BtsI GCAGTG_nn' Cuts at: 2017 3250 4044 4064 2017 Size: 1233 794 20 3687 Fragments arranged by size: 3687 1233 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5699 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2254 2391 Size: 54 1425 50 275 18 366 58 137 Cuts at: 2391 2442 3181 4070 5699 8 Size: 51 739 889 1629 43 Fragments arranged by size: 1629 1425 889 739 366 275 137 58 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3664 1353 Size: 2311 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2644 2680 4321 4695 4841 387 Size: 1064 138 1055 36 1641 374 146 1280 Fragments arranged by size: 1641 1280 1064 1055 374 146 138 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2467 2519 2528 2775 2790 3797 Size: 393 563 272 52 9 247 15 1007 Cuts at: 3797 4394 5181 5449 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1007 787 597 563 393 272 268 247 52 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 2582 2582 Size: 5734 EcoO109I rG'GnC_Cy Cuts at: 2039 2169 2224 2269 2270 2341 2342 2355 2381 Size: 130 55 45 1 71 1 13 26 Cuts at: 2381 2774 2039 Size: 393 4999 Fragments arranged by size: 4999 393 130 71 55 45 26 13 1 1 EcoRI G'AATT_C Cuts at: 3043 3421 3043 Size: 378 5356 FspI TGC'GCA Cuts at: 2570 4240 2570 Size: 1670 4064 HaeII r_GCGC'y Cuts at: 1678 4881 1678 Size: 3203 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2374 2951 2983 4375 Size: 32 135 32 770 32 577 32 1392 Cuts at: 4375 4407 4449 4481 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1392 770 577 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KpnI G_GTAC'C Cuts at: 3059 3059 Size: 5734 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4848 5032 1305 Size: 3543 184 2007 Fragments arranged by size: 3543 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2393 2444 10 Size: 54 1425 343 561 51 3300 Fragments arranged by size: 3300 1425 561 343 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 2696 2835 2841 2861 3692 4051 4210 Size: 648 1530 139 6 20 831 359 159 Cuts at: 4210 518 Size: 2042 Fragments arranged by size: 2042 1530 831 648 359 159 139 20 6 MspA1I CmG'CkG Cuts at: 1606 2105 2136 2143 2276 2320 2497 2560 2728 Size: 499 31 7 133 44 177 63 168 Cuts at: 2728 3150 3827 4975 5220 1606 Size: 422 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 499 422 245 177 168 133 63 44 31 7 NaeI GCC'GGC Cuts at: 2196 2196 Size: 5734 NcoI C'CATG_G Cuts at: 513 1065 2004 2433 2836 2905 513 Size: 552 939 429 403 69 3342 Fragments arranged by size: 3342 939 552 429 403 69 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NgoMIV G'CCGG_C Cuts at: 2194 2194 Size: 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NsiI A_TGCA'T Cuts at: 2816 2816 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 2818 3092 4637 1132 Size: 97 1589 274 1545 2229 Fragments arranged by size: 2229 1589 1545 274 97 PasI CC'CwG_GG Cuts at: 1348 2923 1348 Size: 1575 4159 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 2759 1108 Size: 1651 4083 PfoI T'CCnGG_A Cuts at: 2655 2752 2655 Size: 97 5637 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3803 3835 5316 5348 3803 Size: 32 1481 32 4189 Fragments arranged by size: 4189 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2224 2224 Size: 5734 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5734 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PspOMI G'GGCC_C Cuts at: 2083 2168 2231 2269 2341 2354 2083 Size: 85 63 38 72 13 5463 Fragments arranged by size: 5463 85 72 63 38 13 PstI C_TGCA'G Cuts at: 830 2223 2733 3086 830 Size: 1393 510 353 3478 Fragments arranged by size: 3478 1393 510 353 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 PvuII CAG'CTG Cuts at: 2320 2497 2560 2728 2320 Size: 177 63 168 5326 Fragments arranged by size: 5326 177 168 63 SacII CC_GC'GG Cuts at: 2137 2144 2277 2137 Size: 7 133 5594 Fragments arranged by size: 5594 133 7 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SbfI CC_TGCA'GG Cuts at: 2223 3086 2223 Size: 863 4871 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 2219 2729 3082 4217 4898 5089 826 Size: 219 1174 510 353 1135 681 191 1471 Fragments arranged by size: 1471 1174 1135 681 510 353 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 2418 2552 2591 3061 1872 Size: 546 134 39 470 4545 Fragments arranged by size: 4545 546 470 134 39 SmlI C'TyrA_G Cuts at: 820 1017 1943 3838 4739 5001 820 Size: 197 926 1895 901 262 1553 Fragments arranged by size: 1895 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5734 StyI C'CwwG_G Cuts at: 513 1065 2004 2325 2433 2836 2905 3000 3173 Size: 552 939 321 108 403 69 95 173 Cuts at: 3173 513 Size: 3074 Fragments arranged by size: 3074 939 552 403 321 173 108 95 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3761 3778 3931 4116 4561 325 Size: 1255 2181 17 153 185 445 1498 Fragments arranged by size: 2181 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3980 5692 96 Size: 274 80 33 51 1443 2003 1712 138 Fragments arranged by size: 2003 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5694 5726 5694 Size: 32 5702 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 2416 2550 2589 3059 1870 Size: 546 134 39 470 4545 Fragments arranged by size: 4545 546 470 134 39 XmnI GAAnn'nnTTC Cuts at: 2516 2672 3863 2516 Size: 156 1191 4387 Fragments arranged by size: 4387 1191 156 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI