(Linear) (Six-base) MAPSORT of: KIBB7681.seq Check: 6378 from: 1 to: 1029 KIBB7681.orf With 182 enzymes: SgfI * July 3, 2012 16:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 119 1029 Size: 119 910 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 462 1029 Size: 462 567 AhdI GACnn_n'nnGTC Cuts at: 0 960 1029 Size: 960 69 AlwNI CAG_nnn'CTG Cuts at: 0 754 1029 Size: 754 275 ApaI G_GGCC'C Cuts at: 0 82 167 230 268 340 353 1029 Size: 82 85 63 38 72 13 676 Fragments arranged by size: 676 85 82 72 63 38 13 AvaI C'yCGr_G Cuts at: 0 7 60 223 411 545 584 1029 Size: 7 53 163 188 134 39 445 Fragments arranged by size: 445 188 163 134 53 39 7 BanI G'GyrC_C Cuts at: 0 858 1029 Size: 858 171 BanII G_rGCy'C Cuts at: 0 82 167 176 230 268 340 353 763 Size: 82 85 9 54 38 72 13 410 Cuts at: 763 1029 Size: 266 Fragments arranged by size: 410 266 85 82 72 54 38 13 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 292 504 1029 Size: 292 212 525 Fragments arranged by size: 525 292 212 BbvCI CC'TCA_GC Cuts at: 0 278 719 1029 Size: 278 441 310 Fragments arranged by size: 441 310 278 BfrBI ATG'CAT Cuts at: 0 809 1029 Size: 809 220 BglI GCCn_nnn'nGGC Cuts at: 0 335 767 1029 Size: 335 432 262 Fragments arranged by size: 432 335 262 Bme1580I G_kGCm'C Cuts at: 0 82 167 230 268 340 353 1029 Size: 82 85 63 38 72 13 676 Fragments arranged by size: 676 85 82 72 63 38 13 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 306 960 1029 Size: 306 654 69 Fragments arranged by size: 654 306 69 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 514 523 770 785 1029 Size: 190 324 9 247 15 244 Fragments arranged by size: 324 247 244 190 15 9 Bpu10I CC'TnA_GC Cuts at: 0 278 719 982 1029 Size: 278 441 263 47 Fragments arranged by size: 441 278 263 47 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 295 511 1029 Size: 295 216 518 Fragments arranged by size: 518 295 216 BseYI C'CCAG_C Cuts at: 0 311 1029 Size: 311 718 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 23 97 1029 Size: 23 74 932 Fragments arranged by size: 932 74 23 BsiHKAI G_wGCw'C Cuts at: 0 14 1029 Size: 14 1015 BsmBI CGTCTCn'nnnn_ Cuts at: 0 338 981 1029 Size: 338 643 48 Fragments arranged by size: 643 338 48 Bsp1286I G_dGCh'C Cuts at: 0 14 82 167 176 230 268 340 353 Size: 14 68 85 9 54 38 72 13 Cuts at: 353 763 1029 Size: 410 266 Fragments arranged by size: 410 266 85 72 68 54 38 14 13 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 119 221 1029 Size: 119 102 808 Fragments arranged by size: 808 119 102 BsrFI r'CCGG_y Cuts at: 0 189 1029 Size: 189 840 BssHII G'CGCG_C Cuts at: 0 17 1029 Size: 17 1012 BstXI CCAn_nnnn'nTGG Cuts at: 0 435 832 1029 Size: 435 397 197 Fragments arranged by size: 435 397 197 BstYI r'GATC_y Cuts at: 0 647 751 1029 Size: 647 104 278 Fragments arranged by size: 647 278 104 BtgI C'CryG_G Cuts at: 0 129 136 269 428 831 900 1029 Size: 129 7 133 159 403 69 129 Fragments arranged by size: 403 159 133 129 129 69 7 BtsI GCAGTG_nn' Cuts at: 0 12 1029 Size: 12 1017 EaeI y'GGCC_r Cuts at: 0 191 249 386 437 1029 Size: 191 58 137 51 592 Fragments arranged by size: 592 191 137 58 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 639 675 1029 Size: 639 36 354 Fragments arranged by size: 639 354 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 462 514 523 770 785 1029 Size: 190 272 52 9 247 15 244 Fragments arranged by size: 272 247 244 190 52 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 577 1029 Size: 577 452 EcoO109I rG'GnC_Cy Cuts at: 0 34 164 219 264 265 336 337 350 Size: 34 130 55 45 1 71 1 13 Cuts at: 350 376 769 1029 Size: 26 393 260 Fragments arranged by size: 393 260 130 71 55 45 34 26 13 1 1 FspI TGC'GCA Cuts at: 0 565 1029 Size: 565 464 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 337 369 946 978 1029 Size: 337 32 577 32 51 Fragments arranged by size: 577 337 51 32 32 MscI TGG'CCA Cuts at: 0 388 439 1029 Size: 388 51 590 Fragments arranged by size: 590 388 51 MslI CAynn'nnrTG Cuts at: 0 691 830 836 856 1029 Size: 691 139 6 20 173 Fragments arranged by size: 691 173 139 20 6 MspA1I CmG'CkG Cuts at: 0 100 131 138 271 315 492 555 723 Size: 100 31 7 133 44 177 63 168 Cuts at: 723 1029 Size: 306 Fragments arranged by size: 306 177 168 133 100 63 44 31 7 NaeI GCC'GGC Cuts at: 0 191 1029 Size: 191 838 NcoI C'CATG_G Cuts at: 0 428 831 900 1029 Size: 428 403 69 129 Fragments arranged by size: 428 403 129 69 NgoMIV G'CCGG_C Cuts at: 0 189 1029 Size: 189 840 NsiI A_TGCA'T Cuts at: 0 811 1029 Size: 811 218 NspI r_CATG'y Cuts at: 0 813 1029 Size: 813 216 PasI CC'CwG_GG Cuts at: 0 918 1029 Size: 918 111 PflMI CCAn_nnn'nTGG Cuts at: 0 754 1029 Size: 754 275 PfoI T'CCnGG_A Cuts at: 0 650 747 1029 Size: 650 97 282 Fragments arranged by size: 650 282 97 PpuMI rG'GwC_Cy Cuts at: 0 219 1029 Size: 219 810 PspOMI G'GGCC_C Cuts at: 0 78 163 226 264 336 349 1029 Size: 78 85 63 38 72 13 680 Fragments arranged by size: 680 85 78 72 63 38 13 PstI C_TGCA'G Cuts at: 0 218 728 1029 Size: 218 510 301 Fragments arranged by size: 510 301 218 PvuII CAG'CTG Cuts at: 0 315 492 555 723 1029 Size: 315 177 63 168 306 Fragments arranged by size: 315 306 177 168 63 SacII CC_GC'GG Cuts at: 0 132 139 272 1029 Size: 132 7 133 757 Fragments arranged by size: 757 133 132 7 SbfI CC_TGCA'GG Cuts at: 0 218 1029 Size: 218 811 SfcI C'TryA_G Cuts at: 0 214 724 1029 Size: 214 510 305 Fragments arranged by size: 510 305 214 SmaI CCC'GGG Cuts at: 0 413 547 586 1029 Size: 413 134 39 443 Fragments arranged by size: 443 413 134 39 StyI C'CwwG_G Cuts at: 0 320 428 831 900 995 1029 Size: 320 108 403 69 95 34 Fragments arranged by size: 403 320 108 95 69 34 XmaI C'CCGG_G Cuts at: 0 411 545 584 1029 Size: 411 134 39 445 Fragments arranged by size: 445 411 134 39 XmnI GAAnn'nnTTC Cuts at: 0 511 667 1029 Size: 511 156 362 Fragments arranged by size: 511 362 156 Enzymes that do cut: AarI AcuI AhdI AlwNI ApaI AvaI BanI BanII BbsI BbvCI BfrBI BglI Bme1580I BplI BpmI Bpu10I BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII BstXI BstYI BtgI BtsI EaeI EciI Eco57MI EcoNI EcoO109I FspI Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SbfI SfcI SmaI StyI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI ZraI