################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 3 GTCTAC GT^[AC][GT]AC AcsI 847 AAATTC [GA]^AATT[CT] AlwI 909 GGATC GGATC(4/5) ApaI 183 GGGCCC GGGCC^C ApoI 847 AAATTC [GA]^AATT[CT] BalI 221 TGGCCA TGG^CCA BbsI 1225 GTCTTC GAAGAC(2/6) BcgI 677 GCAAAATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 960 CCCGG CC^[GC]GG BfaI 598 CTAG C^TAG BpuAI 1225 GTCTTC GAAGAC(2/6) BsaI 1170 GGTCTC GGTCTC(1/5) BsaWI 242 TCCGGT [AT]^CCGG[AT] BsmI 1255 GCATTC GAATGC(1/-1) BssSI 895 CTCGTG CACGAG(-5/-1) BstBI 721 TTCGAA TT^CGAA BstXI 351 CCAGAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 1140 TTTAAA TTT^AAA DraIII 84 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 357 CCATGG C^C[GA][CT]GG EaeI 221 TGGCCA [CT]^GGCC[GA] HgaI 207 GCGTC GACGC(5/10) MaeI 598 CTAG C^TAG MluNI 221 TGGCCA TGG^CCA MscI 221 TGGCCA TGG^CCA MseI 1141 TTAA T^TAA NciI 960 CCCGG CC^[GC]GG NcoI 357 CCATGG C^CATGG NspV 721 TTCGAA TT^CGAA PstI 256 CTGCAG CTGCA^G SfuI 721 TTCGAA TT^CGAA StuI 1324 AGGCCT AGG^CCT TaqI 722 TCGA T^CGA Tru9I 1141 TTAA T^TAA Tsp509I 848 AATT ^AATT TthHB8I 722 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 466 GTAC GT^AC 995 GTAC BanII 183 GGGCCC G[GA]GC[CT]^C 746 GAGCCC BbvCI 391 GCTGAGG CCTCAGC(-5/-2) 630 GCTGAGG BglII 12 AGATCT A^GATCT 1001 AGATCT CfoI 310 GCGC GCG^C 338 GCGC EcoNI 27 CCTCAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1180 CCTTTGAAAGG FauI 186 CCCGC CCCGC(4/6) 1296 CCCGC HaeII 309 GGCGCT [GA]GCGC^[CT] 337 AGCGCC HapII 243 CCGG C^CGG 961 CCGG HhaI 310 GCGC GCG^C 338 GCGC HinP1I 310 GCGC G^CGC 338 GCGC HpaII 243 CCGG C^CGG 961 CCGG MaeII 1196 ACGT A^CGT 1400 ACGT MaeIII 79 GTGAC ^GT[ACGT]AC 1176 GTCAC MslI 546 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 822 CACGGGAATG MspI 243 CCGG C^CGG 961 CCGG PpuMI 172 AGGACCT [GA]G^G[AT]CC[CT] 1118 GGGACCT RsaI 466 GTAC GT^AC 995 GTAC SmlI 702 CTCAAG C^T[CT][GA]AG 730 CTTGAG Tsp45I 79 GTGAC ^GT[GC]AC 1176 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AlwNI AocI AvaII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI TspRI XhoII