(Circular) (Six-base) MAPSORT of: pFN21AE4095.seq Check: 7372 from: 1 to: 6109 pFN21AE4095 6109 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2009 3452 1937 Size: 72 1443 4594 Fragments arranged by size: 4594 1443 72 Acc65I G'GTAC_C Cuts at: 3430 3430 Size: 6109 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2591 2765 2939 4172 5556 5824 1632 Size: 959 174 174 1233 1384 268 1917 Fragments arranged by size: 1917 1384 1233 959 268 174 174 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5912 AflIII A'CryG_T Cuts at: 1203 5008 5985 1203 Size: 3805 977 1327 Fragments arranged by size: 3805 1327 977 AgeI A'CCGG_T Cuts at: 4940 4940 Size: 6109 AhdI GACnn_n'nnGTC Cuts at: 4838 4838 Size: 6109 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6109 AlwNI CAG_nnn'CTG Cuts at: 2133 2260 2569 3171 3852 5424 2133 Size: 127 309 602 681 1572 2818 Fragments arranged by size: 2818 1572 681 602 309 127 ApaI G_GGCC'C Cuts at: 2192 2192 Size: 6109 ApaLI G'TGCA_C Cuts at: 4166 5322 4166 Size: 1156 4953 ApoI r'AATT_y Cuts at: 1493 2852 3418 3644 3796 5905 1493 Size: 1359 566 226 152 2109 1697 Fragments arranged by size: 2109 1697 1359 566 226 152 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 3434 1870 Size: 73 1491 4545 Fragments arranged by size: 4545 1491 73 BamHI G'GATC_C Cuts at: 3439 3806 3439 Size: 367 5742 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3430 4885 618 Size: 325 217 627 1643 1455 1842 Fragments arranged by size: 1842 1643 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2192 2755 3428 1391 Size: 801 563 673 4072 Fragments arranged by size: 4072 801 673 563 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3223 928 Size: 971 1324 3814 Fragments arranged by size: 3814 1324 971 BbvCI CC'TCA_GC Cuts at: 2397 2636 2397 Size: 239 5870 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2671 2705 4300 4334 1903 Size: 34 734 34 1595 34 3678 Fragments arranged by size: 3678 1595 734 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 1548 Size: 6109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4720 136 Size: 107 122 71 631 605 3048 1525 Fragments arranged by size: 3048 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2017 3006 6104 2017 Size: 989 3098 2022 Fragments arranged by size: 3098 2022 989 BlpI GC'TnA_GC Cuts at: 3526 3526 Size: 6109 Bme1580I G_kGCm'C Cuts at: 1790 2103 2192 4170 5326 1790 Size: 313 89 1978 1156 2573 Fragments arranged by size: 2573 1978 1156 313 89 BmgBI CAC'GTC Cuts at: 1318 3202 3406 1318 Size: 1884 204 4021 Fragments arranged by size: 4021 1884 204 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2075 3040 3840 Size: 416 53 83 399 24 658 965 800 Cuts at: 3840 4798 442 Size: 958 1753 Fragments arranged by size: 1753 965 958 800 658 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2399 2435 2399 Size: 36 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2194 2371 2405 2579 4769 1239 Size: 955 177 34 174 2190 2579 Fragments arranged by size: 2579 2190 955 177 174 34 Bpu10I CC'TnA_GC Cuts at: 2397 2463 2636 2678 2397 Size: 66 173 42 5828 Fragments arranged by size: 5828 173 66 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2692 2756 4234 5099 5397 2692 Size: 64 1478 865 298 3404 Fragments arranged by size: 3404 1478 865 298 64 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3181 4772 882 Size: 617 1682 1591 2219 Fragments arranged by size: 2219 1682 1591 617 BsaAI yAC'GTr Cuts at: 493 1204 3900 493 Size: 711 2696 2702 Fragments arranged by size: 2702 2696 711 BsaBI GATnn'nnATC Cuts at: 6103 6103 Size: 6109 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3916 4298 4933 275 Size: 53 83 186 936 2383 382 635 1451 Fragments arranged by size: 2383 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2247 4542 4940 5214 5361 5772 2247 Size: 2295 398 274 147 411 2584 Fragments arranged by size: 2584 2295 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5884 Fragments arranged by size: 5884 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2414 2594 2763 1186 Size: 928 300 180 169 4532 Fragments arranged by size: 4532 928 300 180 169 BseYI C'CCAG_C Cuts at: 2133 2661 2878 2995 5312 5808 2133 Size: 528 217 117 2317 496 2434 Fragments arranged by size: 2434 2317 528 496 217 117 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5536 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3559 4320 5348 1540 Size: 275 186 1558 761 1028 2301 Fragments arranged by size: 2301 1558 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4170 4255 5326 4170 Size: 85 1071 4953 Fragments arranged by size: 4953 1071 85 BsmI GAATG_Cn' Cuts at: 3260 3620 3713 3260 Size: 360 93 5656 Fragments arranged by size: 5656 360 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2192 2755 3428 4170 4255 5326 Size: 399 313 89 563 673 742 85 1071 Cuts at: 5326 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 673 563 399 313 89 85 BspEI T'CCGG_A Cuts at: 5772 5772 Size: 6109 BspHI T'CATG_A Cuts at: 3998 5728 3998 Size: 1730 4379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3464 844 Size: 2620 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3996 1394 Size: 429 2173 3507 Fragments arranged by size: 3507 2173 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4604 4778 65 Size: 1183 3356 174 1396 Fragments arranged by size: 3356 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4753 4940 1700 Size: 110 2943 187 2869 Fragments arranged by size: 2943 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6109 BssSI C'ACGA_G Cuts at: 2900 4169 5181 2900 Size: 1269 1012 3828 Fragments arranged by size: 3828 1269 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6109 BstBI TT'CG_AA Cuts at: 2727 4913 2727 Size: 2186 3923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstXI CCAn_nnnn'nTGG Cuts at: 2363 2363 Size: 6109 BstYI r'GATC_y Cuts at: 1972 2017 2914 3006 3439 3806 4191 4208 5660 Size: 45 897 92 433 367 385 17 1452 Cuts at: 5660 5746 5758 6104 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 897 433 385 367 346 92 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 2033 2126 3326 2033 Size: 93 1200 4816 Fragments arranged by size: 4816 1200 93 BtgI C'CryG_G Cuts at: 513 1065 2362 513 Size: 552 1297 4260 Fragments arranged by size: 4260 1297 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2218 487 Size: 683 56 992 4378 Fragments arranged by size: 4378 992 683 56 BtsI GCAGTG_nn' Cuts at: 2304 3625 4419 4439 2304 Size: 1321 794 20 3974 Fragments arranged by size: 3974 1321 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6074 DraI TTT'AAA Cuts at: 3147 3413 3147 Size: 266 5843 DraIII CAC_nnn'GTG Cuts at: 2094 2094 Size: 6109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 3556 4445 Size: 54 1425 50 275 18 396 1330 889 Cuts at: 4445 6074 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1330 889 396 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3556 1537 Size: 275 1744 4090 Fragments arranged by size: 4090 1744 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2571 2607 2915 4039 1353 Size: 1218 36 308 1124 3423 Fragments arranged by size: 3423 1218 1124 308 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4696 5070 5216 387 Size: 1064 138 3107 374 146 1280 Fragments arranged by size: 3107 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2194 2371 2405 2579 2591 2765 2939 Size: 393 562 177 34 174 12 174 174 Cuts at: 2939 4172 4769 5556 5824 1239 Size: 1233 597 787 268 1524 Fragments arranged by size: 1524 1233 787 597 562 393 268 177 174 174 174 34 12 EcoNI CCTnn'n_nnAGG Cuts at: 2036 3189 2036 Size: 1153 4956 EcoO109I rG'GnC_Cy Cuts at: 2178 2188 3124 3322 2178 Size: 10 936 198 4965 Fragments arranged by size: 4965 936 198 10 EcoRI G'AATT_C Cuts at: 3418 3796 3418 Size: 378 5731 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2739 2739 Size: 6109 FspI TGC'GCA Cuts at: 4615 4615 Size: 6109 HaeII r_GCGC'y Cuts at: 1678 2318 2346 5256 1678 Size: 640 28 2910 2531 Fragments arranged by size: 2910 2531 640 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3116 3148 3230 3262 4750 Size: 32 135 32 1544 32 82 32 1488 Cuts at: 4750 4782 4824 4856 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1544 1488 135 82 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3453 3699 669 Size: 988 281 1515 246 3079 Fragments arranged by size: 3079 1515 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6109 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KpnI G_GTAC'C Cuts at: 3434 3434 Size: 6109 MfeI C'AATT_G Cuts at: 3708 3708 Size: 6109 MluI A'CGCG_T Cuts at: 5985 5985 Size: 6109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2889 5223 5407 1305 Size: 1584 2334 184 2007 Fragments arranged by size: 2334 2007 1584 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2228 10 Size: 54 1425 343 396 3891 Fragments arranged by size: 3891 1425 396 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2555 2831 4067 4426 4585 518 Size: 648 1389 276 1236 359 159 2042 Fragments arranged by size: 2042 1389 1236 648 359 276 159 MspA1I CmG'CkG Cuts at: 1606 3525 4202 5350 5595 1606 Size: 1919 677 1148 245 2120 Fragments arranged by size: 2120 1919 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2362 513 Size: 552 1297 4260 Fragments arranged by size: 4260 1297 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 3556 3556 Size: 6109 NspI r_CATG'y Cuts at: 1132 1229 3467 5012 1132 Size: 97 2238 1545 2229 Fragments arranged by size: 2238 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6109 PciI A'CATG_T Cuts at: 5008 5008 Size: 6109 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6109 PfoI T'CCnGG_A Cuts at: 2421 3234 2421 Size: 813 5296 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5691 5723 4178 Size: 32 1481 32 4564 Fragments arranged by size: 4564 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2178 3124 2178 Size: 946 5163 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6109 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PspOMI G'GGCC_C Cuts at: 2188 2188 Size: 6109 PstI C_TGCA'G Cuts at: 830 2265 3461 830 Size: 1435 1196 3478 Fragments arranged by size: 3478 1435 1196 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 4357 6069 4357 Size: 1712 4397 SfcI C'TryA_G Cuts at: 826 1045 2010 2261 3292 3457 4592 5273 5464 Size: 219 965 251 1031 165 1135 681 191 Cuts at: 5464 826 Size: 1471 Fragments arranged by size: 1471 1135 1031 965 681 251 219 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 3436 1872 Size: 1564 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2707 2735 4213 5114 5376 820 Size: 197 926 764 28 1478 901 262 1553 Fragments arranged by size: 1553 1478 926 901 764 262 197 28 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3467 1132 Size: 2335 3774 SspI AAT'ATT Cuts at: 5 52 4033 5 Size: 47 3981 2081 Fragments arranged by size: 3981 2081 47 StuI AGG'CCT Cuts at: 1517 3331 1517 Size: 1814 4295 StyI C'CwwG_G Cuts at: 513 1065 2181 2310 2362 3548 513 Size: 552 1116 129 52 1186 3074 Fragments arranged by size: 3074 1186 1116 552 129 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3071 4136 4153 4306 4491 4936 325 Size: 1255 1491 1065 17 153 185 445 1498 Fragments arranged by size: 1498 1491 1255 1065 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2470 4355 6067 Size: 274 80 33 51 1443 493 1885 1712 Cuts at: 6067 96 Size: 138 Fragments arranged by size: 1885 1712 1443 493 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6069 6101 6069 Size: 32 6077 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6109 XbaI T'CTAG_A Cuts at: 3445 3445 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6109 XmaI C'CCGG_G Cuts at: 1870 3434 1870 Size: 1564 4545 XmnI GAAnn'nnTTC Cuts at: 4238 4238 Size: 6109 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI