################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AhdI AlwNI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbeI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstNI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRII EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HinfI HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI MvaI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Alw44I 1102 GTGCAC G^TGCAC ApaI 172 GGGCCC GGGCC^C ApaLI 1102 GTGCAC G^TGCAC AspHI 1102 GTGCAC G[AT]GC[AT]^C AspI 1003 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 733 CCCGGG C^[CT]CG[GA]G BanI 13 GGTGCC G^G[CT][GA]CC BbrPI 1105 CACGTG CAC^GTG BglII 616 AGATCT A^GATCT BsaAI 1105 CACGTG [CT]AC^GT[GA] BsaI 602 GAGACC GGTCTC(1/5) BsgI 1038 CTGCAC GTGCAG(16/14) BsiHKAI 1102 GTGCAC G[AT]GC[AT]^C BsoBI 733 CCCGGG C^[CT]CG[GA]G BsrBI 937 GAGCGG CCGCTC(-3/-3) BstAPI 1040 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1032 GGTTACC G^GT[ACGT]ACC BstPI 1032 GGTTACC G^GT[ACGT]ACC DraIII 1041 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1032 GGTTACC G^GT[ACGT]ACC EcoRI 495 GAATTC G^AATTC EcoT14I 743 CCAAGG C^C[AT][AT]GG FauI 10 GCGGG CCCGC(4/6) HaeII 335 AGCGCC [GA]GCGC^[CT] HincII 844 GTCAAC GT[CT]^[GA]AC HindII 844 GTCAAC GT[CT]^[GA]AC HphI 360 GGTGA GGTGA(8/7) MseI 346 TTAA T^TAA NspI 228 GCATGT [GA]CATG^[CT] PflFI 1003 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1105 CACGTG CAC^GTG PmlI 1105 CACGTG CAC^GTG ScaI 441 AGTACT AGT^ACT SmaI 733 CCCGGG CCC^GGG StyI 743 CCAAGG C^C[AT][AT]GG TaqI 1001 TCGA T^CGA Tru9I 346 TTAA T^TAA TspRI 764 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 1003 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1001 TCGA T^CGA XcmI 921 CCAAAAGTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 733 CCCGGG C^CCGGG XmaI 733 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AfaI 442 GTAC GT^AC 637 GTAC AflIII 529 ACACGT A^C[GA][CT]GT 1106 ACGTGT AgeI 109 ACCGGT A^CCGGT 893 ACCGGT AlwI 435 GGATC GGATC(4/5) 723 GGATC BanII 172 GGGCCC G[GA]GC[CT]^C 736 GGGCTC BcnI 733 CCCGG CC^[GC]GG 734 CCGGG BsaWI 109 ACCGGT [AT]^CCGG[AT] 893 ACCGGT BsiYI 813 CCAGCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1073 CCATCTGCCGG BslI 813 CCAGCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1073 CCATCTGCCGG BsmAI 322 GAGAC GTCTC(1/5) 602 GAGAC BsmFI 507 GTCCC GGGAC(10/14) 1070 GTCCC BspMI 368 ACCTGC ACCTGC(4/8) 1036 ACCTGC BssKI 733 CCCGG ^CC[ACGT]GG 734 CCGGG BstYI 435 GGATCT [GA]^GATC[CT] 616 AGATCT HaeIII 82 GGCC GG^CC 173 GGCC MflI 435 GGATCT [GA]^GATC[CT] 616 AGATCT MslI 18 CACGTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 981 CATCTGCATG MspA1I 814 CAGCTG C[AC]G^C[GT]G 946 CAGCTG NaeI 627 GCCGGC GCC^GGC 1079 GCCGGC NciI 733 CCCGG CC^[GC]GG 734 CCGGG NgoMIV 627 GCCGGC G^CCGGC 1079 GCCGGC PinAI 109 ACCGGT A^CCGGT 893 ACCGGT PpuMI 365 AGGACCT [GA]G^G[AT]CC[CT] 505 AGGTCCC PstI 943 CTGCAG CTGCA^G 949 CTGCAG PvuII 814 CAGCTG CAG^CTG 946 CAGCTG RsaI 442 GTAC GT^AC 637 GTAC ScrFI 733 CCCGG CC^[ACGT]GG 734 CCGGG SfaNI 991 GATGC GCATC(5/9) 980 GCATC SfcI 943 CTGCAG C^T[GA][CT]AG 949 CTGCAG Tsp45I 239 GTGAC ^GT[GC]AC 308 GTCAC XhoII 435 GGATCT [GA]^GATC[CT] 616 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AcsI AluI ApoI AvaII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI Bsp1286I BsrFI BsrI BstF5I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EcoO109I Fnu4HI FokI HapII HhaI HinP1I HpaII ItaI MaeI MaeII MaeIII MboI MboII MnlI MspI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I TseI Tsp509I