(Circular) (Six-base) MAPSORT of: pFN21AE4070.seq Check: 1674 from: 1 to: 5824 pFN21AE4070 5824 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3167 1937 Size: 1230 4594 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2381 2643 2843 3887 5271 5539 1632 Size: 749 262 200 1044 1384 268 1917 Fragments arranged by size: 1917 1384 1044 749 268 262 200 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 2534 3111 4723 5700 1203 Size: 1331 577 1612 977 1327 Fragments arranged by size: 1612 1331 1327 977 577 AgeI A'CCGG_T Cuts at: 2114 2898 4655 2114 Size: 784 1757 3283 Fragments arranged by size: 3283 1757 784 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5824 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5824 AlwNI CAG_nnn'CTG Cuts at: 3567 5139 3567 Size: 1572 4252 ApaI G_GGCC'C Cuts at: 2181 2181 Size: 5824 ApaLI G'TGCA_C Cuts at: 3107 3881 5037 3107 Size: 774 1156 3894 Fragments arranged by size: 3894 1156 774 ApoI r'AATT_y Cuts at: 1493 2500 2829 2842 3133 3359 3511 5620 1493 Size: 1007 329 13 291 226 152 2109 1697 Fragments arranged by size: 2109 1697 1007 329 291 226 152 13 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2738 3149 1870 Size: 73 795 411 4545 Fragments arranged by size: 4545 795 411 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 3145 4600 618 Size: 325 217 627 231 1127 1455 1842 Fragments arranged by size: 1842 1455 1127 627 325 231 217 BanII G_rGCy'C Cuts at: 1391 2181 2745 3143 1391 Size: 790 564 398 4072 Fragments arranged by size: 4072 790 564 398 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2436 2571 2973 928 Size: 971 537 135 402 3779 Fragments arranged by size: 3779 971 537 402 135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4015 4049 1903 Size: 34 2078 34 3678 Fragments arranged by size: 3678 2078 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4435 136 Size: 107 122 71 631 605 2763 1525 Fragments arranged by size: 2763 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2621 5819 2621 Size: 3198 2626 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2181 3033 3111 3885 5041 1790 Size: 391 852 78 774 1156 2573 Fragments arranged by size: 2573 1156 852 774 391 78 BmgBI CAC'GTC Cuts at: 1318 2025 1318 Size: 707 5117 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3555 4513 442 Size: 416 53 83 399 24 2138 958 1753 Fragments arranged by size: 2138 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2094 2646 3041 4484 1239 Size: 855 552 395 1443 2579 Fragments arranged by size: 2579 1443 855 552 395 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3949 4814 5112 3949 Size: 865 298 4661 Fragments arranged by size: 4661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2601 4487 882 Size: 617 1102 1886 2219 Fragments arranged by size: 2219 1886 1102 617 BsaAI yAC'GTr Cuts at: 493 1204 3112 3615 493 Size: 711 1908 503 2702 Fragments arranged by size: 2702 1908 711 503 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4013 4648 275 Size: 53 83 186 936 2098 382 635 1451 Fragments arranged by size: 2098 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2114 2898 4257 4655 4929 5076 5487 2114 Size: 784 1359 398 274 147 411 2451 Fragments arranged by size: 2451 1359 784 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5599 Fragments arranged by size: 5599 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5824 BseYI C'CCAG_C Cuts at: 5027 5523 5027 Size: 496 5328 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3028 1163 Size: 573 1292 3959 Fragments arranged by size: 3959 1292 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3111 3885 3970 5041 3111 Size: 774 85 1071 3894 Fragments arranged by size: 3894 1071 774 85 BsmI GAATG_Cn' Cuts at: 3335 3428 3335 Size: 93 5731 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2745 3033 3111 3143 3885 3970 Size: 399 391 564 288 78 32 742 85 Cuts at: 3970 5041 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 564 399 391 288 85 78 32 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2382 3050 3179 844 Size: 1538 668 129 3489 Fragments arranged by size: 3489 1538 668 129 BsrBI CCG'CTC Cuts at: 1394 1823 2944 3711 1394 Size: 429 1121 767 3507 Fragments arranged by size: 3507 1121 767 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2114 2632 2898 3084 4468 4655 1700 Size: 110 304 518 266 186 1384 187 2869 Fragments arranged by size: 2869 1384 518 304 266 187 186 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 3051 794 Size: 2257 3567 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 3037 1184 Size: 1853 3971 BstYI r'GATC_y Cuts at: 1972 2440 2621 3154 3521 3906 3923 5375 5461 Size: 468 181 533 367 385 17 1452 86 Cuts at: 5461 5473 5819 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 533 468 385 367 346 181 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5272 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5085 Fragments arranged by size: 5085 683 56 BtsI GCAGTG_nn' Cuts at: 3340 4134 4154 3340 Size: 794 20 5010 Fragments arranged by size: 5010 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DraIII CAC_nnn'GTG Cuts at: 3051 3051 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3271 4160 5789 Size: 54 1425 50 275 18 1441 889 1629 Cuts at: 5789 8 Size: 43 Fragments arranged by size: 1629 1441 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3754 1353 Size: 2401 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2381 2643 2646 2843 3041 3887 Size: 393 462 287 262 3 197 198 846 Cuts at: 3887 4484 5271 5539 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 846 787 597 462 393 287 268 262 198 197 3 EcoO109I rG'GnC_Cy Cuts at: 2177 2371 2511 2177 Size: 194 140 5490 Fragments arranged by size: 5490 194 140 EcoRI G'AATT_C Cuts at: 2500 3133 3511 2500 Size: 633 378 4813 Fragments arranged by size: 4813 633 378 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2344 4971 1678 Size: 666 2627 2531 Fragments arranged by size: 2627 2531 666 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 2600 2632 3058 Size: 32 135 32 677 32 319 32 426 Cuts at: 3058 3090 4465 4497 4539 4571 1373 Size: 32 1375 32 42 32 2626 Fragments arranged by size: 2626 1375 677 426 319 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2851 3168 3414 669 Size: 988 281 913 317 246 3079 Fragments arranged by size: 3079 988 913 317 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4002 Fragments arranged by size: 4002 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2990 3782 4141 4300 518 Size: 648 861 963 792 359 159 2042 Fragments arranged by size: 2042 963 861 792 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2821 2953 3240 3917 5065 5310 1606 Size: 1215 132 287 677 1148 245 2120 Fragments arranged by size: 2120 1215 1148 677 287 245 132 NaeI GCC'GGC Cuts at: 2634 3086 2634 Size: 452 5372 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5272 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NgoMIV G'CCGG_C Cuts at: 2632 3084 2632 Size: 452 5372 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2237 3182 4727 1132 Size: 97 1008 945 1545 2229 Fragments arranged by size: 2229 1545 1008 945 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5824 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PmlI CAC'GTG Cuts at: 3112 3112 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2371 2511 2371 Size: 140 5684 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2177 2177 Size: 5824 PstI C_TGCA'G Cuts at: 830 2952 2958 3176 830 Size: 2122 6 218 3478 Fragments arranged by size: 3478 2122 218 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2821 2953 2821 Size: 132 5692 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 2448 4072 5784 2448 Size: 1624 1712 2488 Fragments arranged by size: 2488 1712 1624 SfcI C'TryA_G Cuts at: 826 1045 2948 2954 3172 4307 4988 5179 826 Size: 219 1903 6 218 1135 681 191 1471 Fragments arranged by size: 1903 1471 1135 681 219 218 191 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 2740 3151 1872 Size: 868 411 4545 Fragments arranged by size: 4545 868 411 SmlI C'TyrA_G Cuts at: 820 1017 1943 3928 4829 5091 820 Size: 197 926 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5824 StyI C'CwwG_G Cuts at: 513 1065 2748 3263 513 Size: 552 1683 515 3074 Fragments arranged by size: 3074 1683 552 515 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3851 3868 4021 4206 4651 325 Size: 1255 2271 17 153 185 445 1498 Fragments arranged by size: 2271 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2446 4070 5782 Size: 274 80 33 51 1443 469 1624 1712 Cuts at: 5782 96 Size: 138 Fragments arranged by size: 1712 1624 1443 469 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2002 2034 2796 2828 5784 5816 2002 Size: 32 762 32 2956 32 2010 Fragments arranged by size: 2956 2010 762 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3011 1316 Size: 1695 4129 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2933 2933 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 2738 3149 1870 Size: 868 411 4545 Fragments arranged by size: 4545 868 411 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI