(Linear) (Six-base) MAPSORT of: KIEE3219.seq Check: 6819 from: 1 to: 1485 KIEE3219.orf With 182 enzymes: SgfI * July 3, 2012 17:28 .. AatII G_ACGT'C Cuts at: 0 1244 1485 Size: 1244 241 AclI AA'CG_TT Cuts at: 0 1364 1485 Size: 1364 121 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 464 694 1485 Size: 464 230 791 Fragments arranged by size: 791 464 230 AfeI AGC'GCT Cuts at: 0 1171 1485 Size: 1171 314 AflIII A'CryG_T Cuts at: 0 479 1073 1232 1485 Size: 479 594 159 253 Fragments arranged by size: 594 479 253 159 AleI CACnn'nnGTG Cuts at: 0 949 1485 Size: 949 536 AlwNI CAG_nnn'CTG Cuts at: 0 183 249 555 1009 1485 Size: 183 66 306 454 476 Fragments arranged by size: 476 454 306 183 66 ApaI G_GGCC'C Cuts at: 0 1018 1485 Size: 1018 467 ApoI r'AATT_y Cuts at: 0 135 1485 Size: 135 1350 BamHI G'GATC_C Cuts at: 0 1292 1485 Size: 1292 193 BanII G_rGCy'C Cuts at: 0 299 1018 1485 Size: 299 719 467 Fragments arranged by size: 719 467 299 BbsI GAAGACnn'nnnn_ Cuts at: 0 375 1485 Size: 375 1110 BbvCI CC'TCA_GC Cuts at: 0 217 719 1485 Size: 217 502 766 Fragments arranged by size: 766 502 217 BfrBI ATG'CAT Cuts at: 0 516 1485 Size: 516 969 BglI GCCn_nnn'nGGC Cuts at: 0 1013 1485 Size: 1013 472 BlpI GC'TnA_GC Cuts at: 0 1108 1485 Size: 1108 377 Bme1580I G_kGCm'C Cuts at: 0 1018 1485 Size: 1018 467 BmgBI CAC'GTC Cuts at: 0 480 1074 1485 Size: 480 594 411 Fragments arranged by size: 594 480 411 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 364 1485 Size: 283 81 1121 Fragments arranged by size: 1121 283 81 Bpu10I CC'TnA_GC Cuts at: 0 217 719 1485 Size: 217 502 766 Fragments arranged by size: 766 502 217 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 917 1485 Size: 917 568 BsaI GGTCTCn'nnnn_ Cuts at: 0 639 1485 Size: 639 846 BsaHI Gr'CG_yC Cuts at: 0 79 1241 1485 Size: 79 1162 244 Fragments arranged by size: 1162 244 79 BsaWI w'CCGG_w Cuts at: 0 1384 1485 Size: 1384 101 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1297 1327 1485 Size: 1297 30 158 Fragments arranged by size: 1297 158 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 175 489 875 900 1485 Size: 175 314 386 25 585 Fragments arranged by size: 585 386 314 175 25 BseYI C'CCAG_C Cuts at: 0 1103 1434 1485 Size: 1103 331 51 Fragments arranged by size: 1103 331 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 346 554 1485 Size: 346 208 931 Fragments arranged by size: 931 346 208 BsiHKAI G_wGCw'C Cuts at: 0 299 1485 Size: 299 1186 BsmBI CGTCTCn'nnnn_ Cuts at: 0 470 1485 Size: 470 1015 Bsp1286I G_dGCh'C Cuts at: 0 299 1018 1485 Size: 299 719 467 Fragments arranged by size: 719 467 299 BspEI T'CCGG_A Cuts at: 0 1384 1485 Size: 1384 101 BspHI T'CATG_A Cuts at: 0 275 1485 Size: 275 1210 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 359 1485 Size: 359 1126 BssSI C'ACGA_G Cuts at: 0 1264 1485 Size: 1264 221 BstAPI GCAn_nnn'nTGC Cuts at: 0 555 1485 Size: 555 930 BstEII G'GTnAC_C Cuts at: 0 628 1485 Size: 628 857 BstXI CCAn_nnnn'nTGG Cuts at: 0 951 1485 Size: 951 534 BstYI r'GATC_y Cuts at: 0 245 551 669 1292 1485 Size: 245 306 118 623 193 Fragments arranged by size: 623 306 245 193 118 BtgI C'CryG_G Cuts at: 0 67 1485 Size: 67 1418 DraI TTT'AAA Cuts at: 0 1317 1485 Size: 1317 168 EaeI y'GGCC_r Cuts at: 0 1035 1485 Size: 1035 450 EarI CTCTTCn'nnn_ Cuts at: 0 683 920 1485 Size: 683 237 565 Fragments arranged by size: 683 565 237 EcoICRI GAG'CTC Cuts at: 0 297 1485 Size: 297 1188 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 364 464 694 1485 Size: 283 81 100 230 791 Fragments arranged by size: 791 283 230 100 81 EcoO109I rG'GnC_Cy Cuts at: 0 37 348 1006 1050 1226 1485 Size: 37 311 658 44 176 259 Fragments arranged by size: 658 311 259 176 44 37 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 430 675 1191 1485 Size: 430 245 516 294 Fragments arranged by size: 516 430 294 245 HaeII r_GCGC'y Cuts at: 0 1173 1485 Size: 1173 312 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 622 654 723 755 895 927 1485 Size: 622 32 69 32 140 32 558 Fragments arranged by size: 622 558 140 69 32 32 32 HincII GTy'rAC Cuts at: 0 393 1485 Size: 393 1092 HindIII A'AGCT_T Cuts at: 0 836 1485 Size: 836 649 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 146 1144 1485 Size: 146 998 341 Fragments arranged by size: 998 341 146 MscI TGG'CCA Cuts at: 0 1037 1485 Size: 1037 448 MslI CAynn'nnrTG Cuts at: 0 21 259 949 1031 1485 Size: 21 238 690 82 454 Fragments arranged by size: 690 454 238 82 21 MspA1I CmG'CkG Cuts at: 0 152 851 1434 1485 Size: 152 699 583 51 Fragments arranged by size: 699 583 152 51 NsiI A_TGCA'T Cuts at: 0 518 1485 Size: 518 967 NspI r_CATG'y Cuts at: 0 258 1070 1236 1485 Size: 258 812 166 249 Fragments arranged by size: 812 258 249 166 PasI CC'CwG_GG Cuts at: 0 52 560 1485 Size: 52 508 925 Fragments arranged by size: 925 508 52 PciI A'CATG_T Cuts at: 0 1232 1485 Size: 1232 253 PflMI CCAn_nnn'nTGG Cuts at: 0 380 491 1485 Size: 380 111 994 Fragments arranged by size: 994 380 111 PfoI T'CCnGG_A Cuts at: 0 248 577 1485 Size: 248 329 908 Fragments arranged by size: 908 329 248 PpuMI rG'GwC_Cy Cuts at: 0 37 348 1485 Size: 37 311 1137 Fragments arranged by size: 1137 311 37 PspOMI G'GGCC_C Cuts at: 0 1014 1485 Size: 1014 471 PstI C_TGCA'G Cuts at: 0 1333 1485 Size: 1333 152 PvuII CAG'CTG Cuts at: 0 152 1434 1485 Size: 152 1282 51 Fragments arranged by size: 1282 152 51 SacI G_AGCT'C Cuts at: 0 299 1485 Size: 299 1186 SapI GCTCTTCn'nnn_ Cuts at: 0 920 1485 Size: 920 565 SfcI C'TryA_G Cuts at: 0 386 1329 1485 Size: 386 943 156 Fragments arranged by size: 943 386 156 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1013 1485 Size: 1013 472 SmlI C'TyrA_G Cuts at: 0 932 1485 Size: 932 553 StuI AGG'CCT Cuts at: 0 1423 1485 Size: 1423 62 StyI C'CwwG_G Cuts at: 0 659 1046 1485 Size: 659 387 439 Fragments arranged by size: 659 439 387 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1043 1393 1485 Size: 1043 350 92 Fragments arranged by size: 1043 350 92 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 657 1485 Size: 657 828 XmnI GAAnn'nnTTC Cuts at: 0 1171 1485 Size: 1171 314 ZraI GAC'GTC Cuts at: 0 1242 1485 Size: 1242 243 Enzymes that do cut: AatII AclI AcuI AfeI AflIII AleI AlwNI ApaI ApoI BamHI BanII BbsI BbvCI BfrBI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BssSI BstAPI BstEII BstXI BstYI BtgI DraI EaeI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NsiI NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI SfcI SfiI SmlI StuI StyI TaqII XcmI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AflII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BcgI BciVI BclI BglII BmrI BmtI BplI BsaAI BsaBI BsiEI BsiWI BsmI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI