################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI AocI Aor51HI ApaI AscI Asp718I AvaI AviII AvrII BamHI BanI BbeI BcgI BclI BcnI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MluI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 2107 GTCTAC GT^[AC][GT]AC AccIII 1997 TCCGGA T^CCGGA AhdI 1574 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 1574 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 115 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2207 TGGCCA TGG^CCA BbrPI 1196 CACGTG CAC^GTG BbvCI 489 GCTGAGG CCTCAGC(-5/-2) BciVI 1438 GGATAC GTATCC(6/5) BfaI 1561 CTAG C^TAG BglI 1274 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 2057 GAGACC GGTCTC(1/5) BsaWI 1997 TCCGGA [AT]^CCGG[AT] BseAI 1997 TCCGGA T^CCGGA BseRI 440 GAGGAG GAGGAG(10/8) BsgI 763 GTGCAG GTGCAG(16/14) BsmBI 1693 CGTCTC CGTCTC(1/5) BspEI 1997 TCCGGA T^CCGGA BspLU11I 1713 ACATGT A^CATGT BspMI 1736 ACCTGC ACCTGC(4/8) BsrBI 484 GAGCGG CCGCTC(-3/-3) BstXI 2198 CCAGTGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 1246 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 681 CCATGG C^C[GA][CT]GG EaeI 2207 TGGCCA [CT]^GGCC[GA] Eam1105I 1574 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 945 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HapII 1998 CCGG C^CGG HincII 112 GTTGAC GT[CT]^[GA]AC HindII 112 GTTGAC GT[CT]^[GA]AC HpaII 1998 CCGG C^CGG MaeI 1561 CTAG C^TAG MfeI 127 CAATTG C^AATTG MluNI 2207 TGGCCA TGG^CCA MroI 1997 TCCGGA T^CCGGA MscI 2207 TGGCCA TGG^CCA MslI 2014 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1998 CCGG C^CGG MunI 127 CAATTG C^AATTG NcoI 681 CCATGG C^CATGG NspI 1713 ACATGT [GA]CATG^[CT] PflFI 115 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1196 CACGTG CAC^GTG PmlI 1196 CACGTG CAC^GTG PshAI 35 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 62 CTGCAG CTGCA^G SexAI 116 ACCTGGT A^CC[AT]GGT SmlI 1081 CTCAAG C^T[CT][GA]AG Tth111I 115 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1560 TCTAGA T^CTAGA XcmI 506 CCACAGCTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 199 GTAC GT^AC 893 GTAC AflIII 537 ACACGT A^C[GA][CT]GT 1713 ACATGT Alw44I 1199 GTGCAC G^TGCAC 1339 GTGCAC AlwNI 1315 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG 1450 CAGGATCTG ApaLI 1199 GTGCAC G^TGCAC 1339 GTGCAC AseI 796 ATTAAT AT^TAAT 1906 ATTAAT AsnI 796 ATTAAT AT^TAAT 1906 ATTAAT BanII 633 GAGCTC G[GA]GC[CT]^C 1017 GAGCTC BglII 1557 AGATCT A^GATCT 2091 AGATCT BsaAI 538 CACGTA [CT]AC^GT[GA] 1196 CACGTG BsmFI 33 GGGAC GGGAC(10/14) 1721 GGGAC BstAPI 295 GCAGCGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 666 GCATCTGCTGC Cac8I 834 GCTTGC GC[ACGT]^[ACGT]GC 2209 GCCAGC EcoT14I 681 CCATGG C^C[AT][AT]GG 1569 CCTTGG FauI 304 GCGGG CCCGC(4/6) 1206 GCGGG HgaI 520 GACGC GACGC(5/10) 1782 GACGC PleI 720 GACTC GAGTC(4/5) 1317 GACTC PshBI 796 ATTAAT AT^TAAT 1906 ATTAAT RsaI 199 GTAC GT^AC 893 GTAC SacI 633 GAGCTC GAGCT^C 1017 GAGCTC StyI 681 CCATGG C^C[AT][AT]GG 1569 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI Asp700I AspHI AvaII BbsI BbvI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI