(Circular) (Six-base) MAPSORT of: pFN21AB5742.seq Check: 15 from: 1 to: 6928 pFN21AB5742 6928 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4738 278 Size: 53 83 186 936 3202 2468 Fragments arranged by size: 3202 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4113 4271 1937 Size: 2176 158 4594 Fragments arranged by size: 4594 2176 158 Acc65I G'GTAC_C Cuts at: 4249 4249 Size: 6928 AclI AA'CG_TT Cuts at: 1563 5055 5428 1563 Size: 3492 373 3063 Fragments arranged by size: 3492 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2879 3024 3065 3111 4991 6375 6643 1632 Size: 1247 145 41 46 1880 1384 268 1917 Fragments arranged by size: 1917 1880 1384 1247 268 145 46 41 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6731 AflIII A'CryG_T Cuts at: 1203 2542 3718 5827 6804 1203 Size: 1339 1176 2109 977 1327 Fragments arranged by size: 2109 1339 1327 1176 977 AgeI A'CCGG_T Cuts at: 5759 5759 Size: 6928 AhdI GACnn_n'nnGTC Cuts at: 3584 5657 3584 Size: 2073 4855 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6928 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6928 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2223 2255 2223 Size: 32 6896 AlwNI CAG_nnn'CTG Cuts at: 3325 3460 4671 6243 3325 Size: 135 1211 1572 4010 Fragments arranged by size: 4010 1572 1211 135 ApaLI G'TGCA_C Cuts at: 3204 3344 4985 6141 3204 Size: 140 1641 1156 3991 Fragments arranged by size: 3991 1641 1156 140 ApoI r'AATT_y Cuts at: 1493 2429 2458 3380 4006 4237 4463 4615 6724 Size: 936 29 922 626 231 226 152 2109 Cuts at: 6724 1493 Size: 1697 Fragments arranged by size: 2109 1697 936 922 626 231 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 2802 3912 5482 160 Size: 872 1770 1110 1570 1606 Fragments arranged by size: 1770 1606 1570 1110 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6928 AvaI C'yCGr_G Cuts at: 1870 1943 4253 1870 Size: 73 2310 4545 Fragments arranged by size: 4545 2310 73 BamHI G'GATC_C Cuts at: 4258 4625 4258 Size: 367 6561 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4249 5704 618 Size: 325 217 627 2462 1455 1842 Fragments arranged by size: 2462 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2642 3026 4247 1391 Size: 1251 384 1221 4072 Fragments arranged by size: 4072 1251 1221 384 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2082 2729 3012 3185 4091 928 Size: 971 183 647 283 173 906 3765 Fragments arranged by size: 3765 971 906 647 283 183 173 BbvCI CC'TCA_GC Cuts at: 2495 2495 Size: 6928 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5119 5153 1903 Size: 34 3182 34 3678 Fragments arranged by size: 3678 3182 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3437 4820 6030 3437 Size: 1383 1210 4335 Fragments arranged by size: 4335 1383 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6928 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3285 5539 136 Size: 107 122 71 631 605 1613 2254 1525 Fragments arranged by size: 2254 1613 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3562 4096 6923 3562 Size: 534 2827 3567 Fragments arranged by size: 3567 2827 534 BlpI GC'TnA_GC Cuts at: 4345 4345 Size: 6928 Bme1580I G_kGCm'C Cuts at: 1790 3208 3348 4989 6145 1790 Size: 1418 140 1641 1156 2573 Fragments arranged by size: 2573 1641 1418 1156 140 BmgBI CAC'GTC Cuts at: 1318 2046 1318 Size: 728 6200 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3040 4659 5617 Size: 416 53 83 399 24 1623 1619 958 Cuts at: 5617 442 Size: 1753 Fragments arranged by size: 1753 1623 1619 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6928 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2035 2801 3558 3651 5588 1239 Size: 796 766 757 93 1937 2579 Fragments arranged by size: 2579 1937 796 766 757 93 Bpu10I CC'TnA_GC Cuts at: 2495 2636 2495 Size: 141 6787 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3071 5053 5918 6216 3071 Size: 1982 865 298 3783 Fragments arranged by size: 3783 1982 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4056 5591 882 Size: 617 2557 1535 2219 Fragments arranged by size: 2557 2219 1535 617 BsaAI yAC'GTr Cuts at: 493 1204 2545 3203 4719 493 Size: 711 1341 658 1516 2702 Fragments arranged by size: 2702 1516 1341 711 658 BsaBI GATnn'nnATC Cuts at: 6922 6922 Size: 6928 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4735 5117 5752 275 Size: 53 83 186 936 3202 382 635 1451 Fragments arranged by size: 3202 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4002 5361 5759 6033 6180 6591 4002 Size: 1359 398 274 147 411 4339 Fragments arranged by size: 4339 1359 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2139 2169 2223 2253 2340 Size: 30 165 30 736 30 54 30 87 Cuts at: 2340 2370 2439 2469 1178 Size: 30 69 30 5637 Fragments arranged by size: 5637 736 165 87 69 54 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2460 1186 Size: 1274 5654 BseYI C'CCAG_C Cuts at: 3745 6131 6627 3745 Size: 2386 496 4046 Fragments arranged by size: 4046 2386 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2789 1163 Size: 573 1053 5302 Fragments arranged by size: 5302 1053 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4378 5139 6167 1540 Size: 275 186 2377 761 1028 2301 Fragments arranged by size: 2377 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2642 2793 3026 3208 3348 4989 5074 6145 2642 Size: 151 233 182 140 1641 85 1071 3425 Fragments arranged by size: 3425 1641 1071 233 182 151 140 85 BsmI GAATG_Cn' Cuts at: 4439 4532 4439 Size: 93 6835 BsmBI CGTCTCn'nnnn_ Cuts at: 3704 3704 Size: 6928 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2642 2793 3026 3208 3348 4247 4989 Size: 399 852 151 233 182 140 899 742 Cuts at: 4989 5074 6145 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 899 852 742 399 233 182 151 140 85 BspEI T'CCGG_A Cuts at: 4002 6591 4002 Size: 2589 4339 BspHI T'CATG_A Cuts at: 4817 6547 4817 Size: 1730 5198 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3750 4283 844 Size: 2906 533 3489 Fragments arranged by size: 3489 2906 533 BsrBI CCG'CTC Cuts at: 1394 1823 2491 4815 1394 Size: 429 668 2324 3507 Fragments arranged by size: 3507 2324 668 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5423 5597 65 Size: 1183 4175 174 1396 Fragments arranged by size: 4175 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5572 5759 1700 Size: 110 3762 187 2869 Fragments arranged by size: 3762 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6928 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6928 BssSI C'ACGA_G Cuts at: 4988 6000 4988 Size: 1012 5916 BstAPI GCAn_nnn'nTGC Cuts at: 794 2306 2677 794 Size: 1512 371 5045 Fragments arranged by size: 5045 1512 371 BstBI TT'CG_AA Cuts at: 5732 5732 Size: 6928 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6928 BstXI CCAn_nnnn'nTGG Cuts at: 4210 4210 Size: 6928 BstYI r'GATC_y Cuts at: 1972 2911 3049 3457 3541 3562 3625 3843 4096 Size: 939 138 408 84 21 63 218 253 Cuts at: 4096 4258 4625 5010 5027 6479 6565 6577 6923 Size: 162 367 385 17 1452 86 12 346 Cuts at: 6923 1972 Size: 1977 Fragments arranged by size: 1977 1452 939 408 385 367 346 253 218 162 138 86 84 63 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2686 513 Size: 552 939 682 4755 Fragments arranged by size: 4755 939 682 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6189 Fragments arranged by size: 6189 683 56 BtsI GCAGTG_nn' Cuts at: 2076 2748 2831 4444 5238 5258 2076 Size: 672 83 1613 794 20 3746 Fragments arranged by size: 3746 1613 794 672 83 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6928 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6893 DraI TTT'AAA Cuts at: 4232 4232 Size: 6928 DraIII CAC_nnn'GTG Cuts at: 3256 3256 Size: 6928 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5935 809 Size: 507 4619 1802 Fragments arranged by size: 4619 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4212 4375 5264 Size: 54 1425 50 275 18 2382 163 889 Cuts at: 5264 6893 8 Size: 1629 43 Fragments arranged by size: 2382 1629 1425 889 275 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4375 1537 Size: 275 2563 4090 Fragments arranged by size: 4090 2563 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2163 3642 3707 4858 1353 Size: 810 1479 65 1151 3423 Fragments arranged by size: 3423 1479 1151 810 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2947 5515 5889 6035 387 Size: 1064 138 1358 2568 374 146 1280 Fragments arranged by size: 2568 1358 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2640 3024 2640 Size: 384 6544 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 2801 2879 3024 3065 3111 3558 Size: 393 403 766 78 145 41 46 447 Cuts at: 3558 3651 4991 5588 6375 6643 1239 Size: 93 1340 597 787 268 1524 Fragments arranged by size: 1524 1340 787 766 597 447 403 393 268 145 93 78 46 41 EcoNI CCTnn'n_nnAGG Cuts at: 2954 2954 Size: 6928 EcoO109I rG'GnC_Cy Cuts at: 2039 3571 3685 2039 Size: 1532 114 5282 Fragments arranged by size: 5282 1532 114 EcoRI G'AATT_C Cuts at: 4237 4615 4237 Size: 378 6550 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2773 2773 Size: 6928 FspI TGC'GCA Cuts at: 5434 5434 Size: 6928 HaeII r_GCGC'y Cuts at: 1678 6075 1678 Size: 4397 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 2340 2372 2614 Size: 32 135 32 430 32 306 32 242 Cuts at: 2614 2646 3570 3602 3715 3747 5569 5601 5643 Size: 32 924 32 113 32 1822 32 42 Cuts at: 5643 5675 1373 Size: 32 2626 Fragments arranged by size: 2626 1822 924 430 306 242 135 113 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2119 4272 4518 669 Size: 988 281 181 2153 246 3079 Fragments arranged by size: 3079 2153 988 281 246 181 HindIII A'AGCT_T Cuts at: 748 748 Size: 6928 HpaI GTT'AAC Cuts at: 4518 4518 Size: 6928 KpnI G_GTAC'C Cuts at: 4253 4253 Size: 6928 MfeI C'AATT_G Cuts at: 2132 4527 2132 Size: 2395 4533 MluI A'CGCG_T Cuts at: 6804 6804 Size: 6928 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3837 6042 6226 1305 Size: 2532 2205 184 2007 Fragments arranged by size: 2532 2205 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4214 10 Size: 54 1425 343 2382 2724 Fragments arranged by size: 2724 2382 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4023 4886 5245 5404 518 Size: 648 2857 863 359 159 2042 Fragments arranged by size: 2857 2042 863 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2055 2402 3211 4344 5021 6169 6414 1606 Size: 449 347 809 1133 677 1148 245 2120 Fragments arranged by size: 2120 1148 1133 809 677 449 347 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2686 513 Size: 552 939 682 4755 Fragments arranged by size: 4755 939 682 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6928 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6928 NotI GC'GGCC_GC Cuts at: 4375 4375 Size: 6928 NspI r_CATG'y Cuts at: 1132 1229 3722 4286 5831 1132 Size: 97 2493 564 1545 2229 Fragments arranged by size: 2493 2229 1545 564 97 PasI CC'CwG_GG Cuts at: 1348 4220 1348 Size: 2872 4056 PciI A'CATG_T Cuts at: 3718 5827 3718 Size: 2109 4819 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6928 PfoI T'CCnGG_A Cuts at: 2163 2163 Size: 6928 PmeI GTTT'AAAC Cuts at: 4232 4232 Size: 6928 PmlI CAC'GTG Cuts at: 3203 3203 Size: 6928 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2223 2255 4997 5029 6510 6542 2223 Size: 32 2742 32 1481 32 2609 Fragments arranged by size: 2742 2609 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2039 3571 3685 2039 Size: 1532 114 5282 Fragments arranged by size: 5282 1532 114 PshAI GACnn'nnGTC Cuts at: 1613 2044 1613 Size: 431 6497 PsiI TTA'TAA Cuts at: 2439 4498 2439 Size: 2059 4869 PstI C_TGCA'G Cuts at: 830 2071 4280 830 Size: 1241 2209 3478 Fragments arranged by size: 3478 2209 1241 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6928 SacI G_AGCT'C Cuts at: 2642 3026 2642 Size: 384 6544 SalI G'TCGA_C Cuts at: 1936 4270 1936 Size: 2334 4594 SanDI GG'GwC_CC Cuts at: 2039 2039 Size: 6928 SbfI CC_TGCA'GG Cuts at: 4280 4280 Size: 6928 ScaI AGT'ACT Cuts at: 5176 6888 5176 Size: 1712 5216 SexAI A'CCwGG_T Cuts at: 2121 2121 Size: 6928 SfcI C'TryA_G Cuts at: 826 1045 2067 3781 4124 4276 5411 6092 6283 Size: 219 1022 1714 343 152 1135 681 191 Cuts at: 6283 826 Size: 1471 Fragments arranged by size: 1714 1471 1135 1022 681 343 219 191 152 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6928 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6928 SmaI CCC'GGG Cuts at: 1872 4255 1872 Size: 2383 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3086 5032 5933 6195 820 Size: 197 926 1143 1946 901 262 1553 Fragments arranged by size: 1946 1553 1143 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4719 493 Size: 4226 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6928 SphI G_CATG'C Cuts at: 1132 4286 1132 Size: 3154 3774 SspI AAT'ATT Cuts at: 5 52 4852 5 Size: 47 4800 2081 Fragments arranged by size: 4800 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6928 StyI C'CwwG_G Cuts at: 513 1065 2004 2686 3574 4367 513 Size: 552 939 682 888 793 3074 Fragments arranged by size: 3074 939 888 793 682 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3464 3971 4212 4955 4972 5125 5310 Size: 1255 1884 507 241 743 17 153 185 Cuts at: 5310 5755 325 Size: 445 1498 Fragments arranged by size: 1884 1498 1255 743 507 445 241 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2203 2897 5174 Size: 274 80 33 51 1443 226 694 2277 Cuts at: 5174 6886 96 Size: 1712 138 Fragments arranged by size: 2277 1712 1443 694 274 226 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2439 2471 6888 6920 2439 Size: 32 4417 32 2447 Fragments arranged by size: 4417 2447 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2123 1316 Size: 807 6121 XbaI T'CTAG_A Cuts at: 3565 4264 3565 Size: 699 6229 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2518 2518 Size: 6928 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6928 XmaI C'CCGG_G Cuts at: 1870 4253 1870 Size: 2383 4545 XmnI GAAnn'nnTTC Cuts at: 2333 2988 3182 5057 2333 Size: 655 194 1875 4204 Fragments arranged by size: 4204 1875 655 194 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4736 276 Size: 53 83 186 936 3202 2468 Fragments arranged by size: 3202 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SapI SfiI SfoI SrfI SwaI