(Linear) (Six-base) MAPSORT of: KIBB5742.seq Check: 3808 from: 1 to: 2223 KIBB5742.orf With 182 enzymes: SgfI * July 3, 2012 16:04 .. AccI GT'mk_AC Cuts at: 0 2108 2223 Size: 2108 115 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 874 1019 1060 1106 2223 Size: 874 145 41 46 1117 Fragments arranged by size: 1117 874 145 46 41 AflIII A'CryG_T Cuts at: 0 537 1713 2223 Size: 537 1176 510 Fragments arranged by size: 1176 537 510 AhdI GACnn_n'nnGTC Cuts at: 0 1579 2223 Size: 1579 644 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 218 250 2223 Size: 218 32 1973 Fragments arranged by size: 1973 218 32 AlwNI CAG_nnn'CTG Cuts at: 0 1320 1455 2223 Size: 1320 135 768 Fragments arranged by size: 1320 768 135 ApaLI G'TGCA_C Cuts at: 0 1199 1339 2223 Size: 1199 140 884 Fragments arranged by size: 1199 884 140 ApoI r'AATT_y Cuts at: 0 424 453 1375 2001 2223 Size: 424 29 922 626 222 Fragments arranged by size: 922 626 424 222 29 AseI AT'TA_AT Cuts at: 0 797 1907 2223 Size: 797 1110 316 Fragments arranged by size: 1110 797 316 BanII G_rGCy'C Cuts at: 0 637 1021 2223 Size: 637 384 1202 Fragments arranged by size: 1202 637 384 BbsI GAAGACnn'nnnn_ Cuts at: 0 77 724 1007 1180 2086 2223 Size: 77 647 283 173 906 137 Fragments arranged by size: 906 647 283 173 137 77 BbvCI CC'TCA_GC Cuts at: 0 490 2223 Size: 490 1733 BciVI GTATCCnnnnn_n' Cuts at: 0 1432 2223 Size: 1432 791 BglI GCCn_nnn'nGGC Cuts at: 0 1280 2223 Size: 1280 943 BglII A'GATC_T Cuts at: 0 1557 2091 2223 Size: 1557 534 132 Fragments arranged by size: 1557 534 132 Bme1580I G_kGCm'C Cuts at: 0 1203 1343 2223 Size: 1203 140 880 Fragments arranged by size: 1203 880 140 BmgBI CAC'GTC Cuts at: 0 41 2223 Size: 41 2182 BmrI ACTGGGnnnn_n' Cuts at: 0 1035 2223 Size: 1035 1188 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 796 1553 1646 2223 Size: 30 766 757 93 577 Fragments arranged by size: 766 757 577 93 30 Bpu10I CC'TnA_GC Cuts at: 0 490 631 2223 Size: 490 141 1592 Fragments arranged by size: 1592 490 141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1066 2223 Size: 1066 1157 BsaI GGTCTCn'nnnn_ Cuts at: 0 2051 2223 Size: 2051 172 BsaAI yAC'GTr Cuts at: 0 540 1198 2223 Size: 540 658 1025 Fragments arranged by size: 1025 658 540 BsaWI w'CCGG_w Cuts at: 0 1997 2223 Size: 1997 226 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 134 164 218 248 335 365 434 464 Size: 134 30 54 30 87 30 69 30 Cuts at: 464 2223 Size: 1759 Fragments arranged by size: 1759 134 87 69 54 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 455 2223 Size: 455 1768 BseYI C'CCAG_C Cuts at: 0 1740 2223 Size: 1740 483 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 784 2223 Size: 784 1439 BsiHKAI G_wGCw'C Cuts at: 0 637 788 1021 1203 1343 2223 Size: 637 151 233 182 140 880 Fragments arranged by size: 880 637 233 182 151 140 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1699 2223 Size: 1699 524 Bsp1286I G_dGCh'C Cuts at: 0 637 788 1021 1203 1343 2223 Size: 637 151 233 182 140 880 Fragments arranged by size: 880 637 233 182 151 140 BspEI T'CCGG_A Cuts at: 0 1997 2223 Size: 1997 226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1745 2223 Size: 1745 478 BsrBI CCG'CTC Cuts at: 0 486 2223 Size: 486 1737 BstAPI GCAn_nnn'nTGC Cuts at: 0 301 672 2223 Size: 301 371 1551 Fragments arranged by size: 1551 371 301 BstXI CCAn_nnnn'nTGG Cuts at: 0 2205 2223 Size: 2205 18 BstYI r'GATC_y Cuts at: 0 906 1044 1452 1536 1557 1620 1838 2091 Size: 906 138 408 84 21 63 218 253 Cuts at: 2091 2223 Size: 132 Fragments arranged by size: 906 408 253 218 138 132 84 63 21 BtgI C'CryG_G Cuts at: 0 681 2223 Size: 681 1542 BtsI GCAGTG_nn' Cuts at: 0 71 743 826 2223 Size: 71 672 83 1397 Fragments arranged by size: 1397 672 83 71 DraIII CAC_nnn'GTG Cuts at: 0 1251 2223 Size: 1251 972 EaeI y'GGCC_r Cuts at: 0 2207 2223 Size: 2207 16 EarI CTCTTCn'nnn_ Cuts at: 0 158 1637 1702 2223 Size: 158 1479 65 521 Fragments arranged by size: 1479 521 158 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 942 2223 Size: 942 1281 EcoICRI GAG'CTC Cuts at: 0 635 1019 2223 Size: 635 384 1204 Fragments arranged by size: 1204 635 384 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 796 874 1019 1060 1106 1553 1646 Size: 30 766 78 145 41 46 447 93 Cuts at: 1646 2223 Size: 577 Fragments arranged by size: 766 577 447 145 93 78 46 41 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 949 2223 Size: 949 1274 EcoO109I rG'GnC_Cy Cuts at: 0 34 1566 1680 2223 Size: 34 1532 114 543 Fragments arranged by size: 1532 543 114 34 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 768 2223 Size: 768 1455 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 29 335 367 609 641 1565 1597 1710 Size: 29 306 32 242 32 924 32 113 Cuts at: 1710 1742 2223 Size: 32 481 Fragments arranged by size: 924 481 306 242 113 32 32 32 32 29 HincII GTy'rAC Cuts at: 0 114 2223 Size: 114 2109 MfeI C'AATT_G Cuts at: 0 127 2223 Size: 127 2096 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1832 2223 Size: 1832 391 MscI TGG'CCA Cuts at: 0 2209 2223 Size: 2209 14 MslI CAynn'nnrTG Cuts at: 0 2018 2223 Size: 2018 205 MspA1I CmG'CkG Cuts at: 0 50 397 1206 2223 Size: 50 347 809 1017 Fragments arranged by size: 1017 809 347 50 NcoI C'CATG_G Cuts at: 0 681 2223 Size: 681 1542 NspI r_CATG'y Cuts at: 0 1717 2223 Size: 1717 506 PasI CC'CwG_GG Cuts at: 0 2215 2223 Size: 2215 8 PciI A'CATG_T Cuts at: 0 1713 2223 Size: 1713 510 PfoI T'CCnGG_A Cuts at: 0 158 2223 Size: 158 2065 PmlI CAC'GTG Cuts at: 0 1198 2223 Size: 1198 1025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 218 250 2223 Size: 218 32 1973 Fragments arranged by size: 1973 218 32 PpuMI rG'GwC_Cy Cuts at: 0 34 1566 1680 2223 Size: 34 1532 114 543 Fragments arranged by size: 1532 543 114 34 PshAI GACnn'nnGTC Cuts at: 0 39 2223 Size: 39 2184 PsiI TTA'TAA Cuts at: 0 434 2223 Size: 434 1789 PstI C_TGCA'G Cuts at: 0 66 2223 Size: 66 2157 SacI G_AGCT'C Cuts at: 0 637 1021 2223 Size: 637 384 1202 Fragments arranged by size: 1202 637 384 SanDI GG'GwC_CC Cuts at: 0 34 2223 Size: 34 2189 SexAI A'CCwGG_T Cuts at: 0 116 2223 Size: 116 2107 SfcI C'TryA_G Cuts at: 0 62 1776 2119 2223 Size: 62 1714 343 104 Fragments arranged by size: 1714 343 104 62 SmlI C'TyrA_G Cuts at: 0 1081 2223 Size: 1081 1142 StyI C'CwwG_G Cuts at: 0 681 1569 2223 Size: 681 888 654 Fragments arranged by size: 888 681 654 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1459 1966 2207 2223 Size: 1459 507 241 16 Fragments arranged by size: 1459 507 241 16 TatI w'GTAC_w Cuts at: 0 198 892 2223 Size: 198 694 1331 Fragments arranged by size: 1331 694 198 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 434 466 2223 Size: 434 32 1757 Fragments arranged by size: 1757 434 32 Tth111I GACn'n_nGTC Cuts at: 0 118 2223 Size: 118 2105 XbaI T'CTAG_A Cuts at: 0 1560 2223 Size: 1560 663 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 513 2223 Size: 513 1710 XmnI GAAnn'nnTTC Cuts at: 0 328 983 1177 2223 Size: 328 655 194 1046 Fragments arranged by size: 1046 655 328 194 Enzymes that do cut: AccI AcuI AflIII AhdI AloI AlwNI ApaLI ApoI AseI BanII BbsI BbvCI BciVI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BstAPI BstXI BstYI BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MfeI MmeI MscI MslI MspA1I NcoI NspI PasI PciI PfoI PmlI PpiI PpuMI PshAI PsiI PstI SacI SanDI SexAI SfcI SmlI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AleI AlfI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI BclI BfrBI BlpI BmtI BplI BsaBI BsaHI BsiEI BsiWI BsmI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XhoI XmaI ZraI