################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AccI AccII AccIII AclI AflII AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp700I AspEI AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiWI BsmBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CpoI DraI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I RcaI RsrII SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI SwaI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 617 GACGTC GACGT^C Acc65I 480 GGTACC G^GTACC Alw44I 401 GTGCAC G^TGCAC Aor51HI 425 AGCGCT AGC^GCT ApaLI 401 GTGCAC G^TGCAC Asp718I 480 GGTACC G^GTACC AspI 1729 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 698 CCCGGG C^[CT]CG[GA]G AviII 384 TGCGCA TGC^GCA BsiEI 657 CGATCG CG[GA][CT]^CG BsoBI 698 CCCGGG C^[CT]CG[GA]G BspDI 655 ATCGAT AT^CGAT BssSI 409 CACGAG CACGAG(-5/-1) ClaI 655 ATCGAT AT^CGAT DrdI 547 GACTTGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 425 AGCGCT AGC^GCT EcoT14I 833 CCATGG C^C[AT][AT]GG FspI 384 TGCGCA TGC^GCA HindIII 463 AAGCTT A^AGCTT KpnI 480 GGTACC GGTAC^C MaeII 618 ACGT A^CGT MseI 851 TTAA T^TAA NcoI 833 CCATGG C^CATGG PflFI 1729 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 657 CGATCG CGAT^CG SacI 623 GAGCTC GAGCT^C SapI 107 GCTCTTC GCTCTTC(1/4) SfiI 1126 GGCCTGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 698 CCCGGG CCC^GGG SmlI 1182 CTTGAG C^T[CT][GA]AG StuI 1125 AGGCCT AGG^CCT StyI 833 CCATGG C^C[AT][AT]GG TfiI 1060 GATTC G^A[AT]TC Tru9I 851 TTAA T^TAA Tth111I 1729 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 698 CCCGGG C^CCGGG XmaI 698 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 22 AAATTT [GA]^AATT[CT] 847 GAATTT AcyI 529 GACGCC G[GA]^CG[CT]C 617 GACGTC ApoI 22 AAATTT [GA]^AATT[CT] 847 GAATTT AspHI 401 GTGCAC G[AT]GC[AT]^C 623 GAGCTC BalI 1001 TGGCCA TGG^CCA 1310 TGGCCA BanII 224 GGGCTC G[GA]GC[CT]^C 623 GAGCTC BbsI 271 GTCTTC GAAGAC(2/6) 1497 GTCTTC BbvCI 1257 CCTCAGC CCTCAGC(-5/-2) 630 GCTGAGG BpuAI 271 GTCTTC GAAGAC(2/6) 1497 GTCTTC BsaHI 529 GACGCC G[GA]^CG[CT]C 617 GACGTC BsaI 742 GAGACC GGTCTC(1/5) 54 GGTCTC BsiHKAI 401 GTGCAC G[AT]GC[AT]^C 623 GAGCTC BsmI 1368 GAATGC GAATGC(1/-1) 793 GCATTC BspMI 745 ACCTGC ACCTGC(4/8) 565 GCAGGT BsrFI 572 GCCGGT [GA]^CCGG[CT] 1761 GCCGGT BstAPI 681 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 767 GCATCAAGTGC Cfr10I 572 GCCGGT [GA]^CCGG[CT] 1761 GCCGGT DsaI 315 CCGTGG C^C[GA][CT]GG 833 CCATGG HaeII 236 AGCGCC [GA]GCGC^[CT] 425 AGCGCT HgaI 529 GACGC GACGC(5/10) 1462 GACGC Hin1I 529 GACGCC G[GA]^CG[CT]C 617 GACGTC MluNI 1001 TGGCCA TGG^CCA 1310 TGGCCA MscI 1001 TGGCCA TGG^CCA 1310 TGGCCA MslI 300 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1440 CACCTCAGTG SpeI 1093 ACTAGT A^CTAGT 1291 ACTAGT Tsp509I 23 AATT ^AATT 848 AATT XcmI 833 CCATGGGCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1323 CCACAGCTATTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanI BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I XhoII