(Circular) (Six-base) MAPSORT of: pFN21AE3253.seq Check: 9119 from: 1 to: 6472 pFN21AE3253 6472 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2561 2561 Size: 6472 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2626 4282 278 Size: 53 83 186 936 1090 1656 2468 Fragments arranged by size: 2468 1656 1090 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3815 1937 Size: 1878 4594 Acc65I G'GTAC_C Cuts at: 2485 3793 2485 Size: 1308 5164 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2243 2628 4535 5919 6187 1632 Size: 611 385 1907 1384 268 1917 Fragments arranged by size: 1917 1907 1384 611 385 268 AfeI AGC'GCT Cuts at: 2432 2432 Size: 6472 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6275 AflIII A'CryG_T Cuts at: 1203 5371 6348 1203 Size: 4168 977 1327 Fragments arranged by size: 4168 1327 977 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 5201 5201 Size: 6472 AleI CACnn'nnGTG Cuts at: 1166 2309 3449 1166 Size: 1143 1140 4189 Fragments arranged by size: 4189 1143 1140 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2421 3467 1184 Size: 1237 1046 4189 Fragments arranged by size: 4189 1237 1046 AlwNI CAG_nnn'CTG Cuts at: 2564 2692 3535 4215 5787 2564 Size: 128 843 680 1572 3249 Fragments arranged by size: 3249 1572 843 680 128 ApaLI G'TGCA_C Cuts at: 2406 4529 5685 2406 Size: 2123 1156 3193 Fragments arranged by size: 3193 2123 1156 ApoI r'AATT_y Cuts at: 1493 2027 2852 3781 4007 4159 6268 1493 Size: 534 825 929 226 152 2109 1697 Fragments arranged by size: 2109 1697 929 825 534 226 152 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 2703 3797 1870 Size: 73 760 1094 4545 Fragments arranged by size: 4545 1094 760 73 BamHI G'GATC_C Cuts at: 3802 4169 3802 Size: 367 6105 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2085 2485 3443 3604 3793 Size: 325 217 627 298 400 958 161 189 Cuts at: 3793 5248 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 958 627 400 325 298 217 189 161 BanII G_rGCy'C Cuts at: 1391 2233 2632 3791 1391 Size: 842 399 1159 4072 Fragments arranged by size: 4072 1159 842 399 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2269 3495 928 Size: 971 370 1226 3905 Fragments arranged by size: 3905 1226 971 370 BbvCI CC'TCA_GC Cuts at: 2636 3263 2636 Size: 627 5845 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4663 4697 1903 Size: 34 2726 34 3678 Fragments arranged by size: 3678 2726 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4364 5574 4364 Size: 1210 5262 BclI T'GATC_A Cuts at: 1548 1548 Size: 6472 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2325 2769 3138 Size: 107 122 71 631 605 653 444 369 Cuts at: 3138 3441 5083 136 Size: 303 1642 1525 Fragments arranged by size: 1642 1525 653 631 605 444 369 303 122 107 71 BglII A'GATC_T Cuts at: 6467 6467 Size: 6472 BlpI GC'TnA_GC Cuts at: 3889 3889 Size: 6472 Bme1580I G_kGCm'C Cuts at: 1790 2316 2410 3098 4533 5689 1790 Size: 526 94 688 1435 1156 2573 Fragments arranged by size: 2573 1435 1156 688 526 94 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6472 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2019 4203 5161 Size: 416 53 83 399 24 602 2184 958 Cuts at: 5161 442 Size: 1753 Fragments arranged by size: 2184 1753 958 602 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2068 2068 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2960 3512 3683 5132 1239 Size: 1721 552 171 1449 2579 Fragments arranged by size: 2579 1721 1449 552 171 Bpu10I CC'TnA_GC Cuts at: 2636 3263 2636 Size: 627 5845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3208 4597 5462 5760 3208 Size: 1389 865 298 3920 Fragments arranged by size: 3920 1389 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2065 2741 5135 882 Size: 617 566 676 2394 2219 Fragments arranged by size: 2394 2219 676 617 566 BsaAI yAC'GTr Cuts at: 493 1204 4263 493 Size: 711 3059 2702 Fragments arranged by size: 3059 2702 711 BsaBI GATnn'nnATC Cuts at: 6466 6466 Size: 6472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2535 2623 4279 4661 Size: 53 83 186 936 1002 88 1656 382 Cuts at: 4661 5296 275 Size: 635 1451 Fragments arranged by size: 1656 1451 1002 936 635 382 186 88 83 53 BsaWI w'CCGG_w Cuts at: 4905 5303 5577 5724 6135 4905 Size: 398 274 147 411 5242 Fragments arranged by size: 5242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6247 Fragments arranged by size: 6247 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2044 2053 2056 2059 2062 2156 2456 2561 Size: 858 9 3 3 3 94 300 105 Cuts at: 2561 2684 2993 3233 3236 3245 1186 Size: 123 309 240 3 9 4413 Fragments arranged by size: 4413 858 309 300 240 123 105 94 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2315 2480 2611 2966 3031 3458 3742 5675 6171 Size: 165 131 355 65 427 284 1933 496 Cuts at: 6171 2315 Size: 2616 Fragments arranged by size: 2616 1933 496 427 355 284 165 131 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5899 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2665 3922 4683 5711 1540 Size: 275 186 664 1257 761 1028 2301 Fragments arranged by size: 2301 1257 1028 761 664 275 186 BsiHKAI G_wGCw'C Cuts at: 2410 2632 4533 4618 5689 2410 Size: 222 1901 85 1071 3193 Fragments arranged by size: 3193 1901 1071 222 85 BsmI GAATG_Cn' Cuts at: 2798 3379 3983 4076 2798 Size: 581 604 93 5194 Fragments arranged by size: 5194 604 581 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2316 2410 2632 3098 3791 4533 Size: 399 443 83 94 222 466 693 742 Cuts at: 4533 4618 5689 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 693 466 443 399 222 94 85 83 BspEI T'CCGG_A Cuts at: 6135 6135 Size: 6472 BspHI T'CATG_A Cuts at: 4361 6091 4361 Size: 1730 4742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2561 2759 3827 844 Size: 1717 198 1068 3489 Fragments arranged by size: 3489 1717 1068 198 BsrBI CCG'CTC Cuts at: 1394 1823 4359 1394 Size: 429 2536 3507 Fragments arranged by size: 3507 2536 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4967 5141 65 Size: 1183 3719 174 1396 Fragments arranged by size: 3719 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2577 3766 5116 5303 1700 Size: 110 767 1189 1350 187 2869 Fragments arranged by size: 2869 1350 1189 767 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6472 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 2414 4532 5544 2414 Size: 2118 1012 3342 Fragments arranged by size: 3342 2118 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2692 2778 794 Size: 1898 86 4488 Fragments arranged by size: 4488 1898 86 BstBI TT'CG_AA Cuts at: 5276 5276 Size: 6472 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6472 BstYI r'GATC_y Cuts at: 1972 2733 3490 3706 3802 4169 4554 4571 6023 Size: 761 757 216 96 367 385 17 1452 Cuts at: 6023 6109 6121 6467 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 761 757 385 367 346 216 96 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2320 2838 513 Size: 552 1255 518 4147 Fragments arranged by size: 4147 1255 552 518 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5733 Fragments arranged by size: 5733 683 56 BtsI GCAGTG_nn' Cuts at: 2468 2690 2722 3988 4782 4802 2468 Size: 222 32 1266 794 20 4138 Fragments arranged by size: 4138 1266 794 222 32 20 ClaI AT'CG_AT Cuts at: 1554 2661 1554 Size: 1107 5365 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2342 2377 529 Size: 35 1778 35 4624 Fragments arranged by size: 4624 1778 35 35 DraI TTT'AAA Cuts at: 3776 3776 Size: 6472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2558 5479 809 Size: 507 1242 2921 1802 Fragments arranged by size: 2921 1802 1242 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2575 3006 3315 Size: 54 1425 50 275 18 745 431 309 Cuts at: 3315 3472 3919 4808 6437 8 Size: 157 447 889 1629 43 Fragments arranged by size: 1629 1425 889 745 447 431 309 275 157 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3919 1537 Size: 275 2107 4090 Fragments arranged by size: 4090 2107 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2015 2080 2119 3222 4402 1353 Size: 662 65 39 1103 1180 3423 Fragments arranged by size: 3423 1180 1103 662 65 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2414 5059 5433 5579 387 Size: 1064 138 825 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 825 374 146 138 EcoICRI GAG'CTC Cuts at: 2630 2630 Size: 6472 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2628 2960 3512 3683 4535 5132 Size: 393 611 385 332 552 171 852 597 Cuts at: 5132 5919 6187 1239 Size: 787 268 1524 Fragments arranged by size: 1524 852 787 611 597 552 393 385 332 268 171 EcoO109I rG'GnC_Cy Cuts at: 2150 2944 2983 3554 2150 Size: 794 39 571 5068 Fragments arranged by size: 5068 794 571 39 EcoRI G'AATT_C Cuts at: 3781 4159 3781 Size: 378 6094 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3452 3452 Size: 6472 FspI TGC'GCA Cuts at: 2391 4978 2391 Size: 2587 3885 HaeII r_GCGC'y Cuts at: 1678 2245 2434 5619 1678 Size: 567 189 3185 2531 Fragments arranged by size: 3185 2531 567 189 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 2522 2554 5113 Size: 32 135 32 467 32 451 32 2559 Cuts at: 5113 5145 5187 5219 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2559 467 451 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3816 4062 669 Size: 988 281 1878 246 3079 Fragments arranged by size: 3079 1878 988 281 246 HindIII A'AGCT_T Cuts at: 748 2468 748 Size: 1720 4752 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KpnI G_GTAC'C Cuts at: 2489 3797 2489 Size: 1308 5164 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2430 2562 2889 5586 5770 1305 Size: 1125 132 327 2697 184 2007 Fragments arranged by size: 2697 2007 1125 327 184 132 MscI TGG'CCA Cuts at: 10 64 1489 1832 3008 3317 10 Size: 54 1425 343 1176 309 3165 Fragments arranged by size: 3165 1425 1176 343 309 54 MslI CAynn'nnrTG Cuts at: 518 1166 2309 3449 4430 4789 4948 518 Size: 648 1143 1140 981 359 159 2042 Fragments arranged by size: 2042 1143 1140 981 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2292 2480 2757 3045 3267 3281 3321 Size: 432 254 188 277 288 222 14 40 Cuts at: 3321 3393 3888 4565 5713 5958 1606 Size: 72 495 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 495 432 288 277 254 245 222 188 72 40 14 NcoI C'CATG_G Cuts at: 513 1065 2838 513 Size: 552 1773 4147 Fragments arranged by size: 4147 1773 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 3830 5375 1132 Size: 97 2601 1545 2229 Fragments arranged by size: 2601 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2154 2953 1348 Size: 806 799 4867 Fragments arranged by size: 4867 806 799 PciI A'CATG_T Cuts at: 5371 5371 Size: 6472 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6472 PfoI T'CCnGG_A Cuts at: 3363 3660 3363 Size: 297 6175 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4541 4573 6054 6086 4541 Size: 32 1481 32 4927 Fragments arranged by size: 4927 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 4042 4042 Size: 6472 PstI C_TGCA'G Cuts at: 830 2463 2688 2756 2762 2819 2907 3050 3824 Size: 1633 225 68 6 57 88 143 774 Cuts at: 3824 830 Size: 3478 Fragments arranged by size: 3478 1633 774 225 143 88 68 57 6 PvuI CG_AT'CG Cuts at: 2001 2665 2001 Size: 664 5808 PvuII CAG'CTG Cuts at: 2038 2480 2757 3045 3267 3281 3321 3393 2038 Size: 442 277 288 222 14 40 72 5117 Fragments arranged by size: 5117 442 288 277 222 72 40 14 SacI G_AGCT'C Cuts at: 2632 2632 Size: 6472 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SapI GCTCTTCn'nnn_ Cuts at: 2119 2119 Size: 6472 SbfI CC_TGCA'GG Cuts at: 3824 3824 Size: 6472 ScaI AGT'ACT Cuts at: 4720 6432 4720 Size: 1712 4760 SfcI C'TryA_G Cuts at: 826 1045 2459 2600 2684 2752 2758 2815 2903 Size: 219 1414 141 84 68 6 57 88 Cuts at: 2903 3046 3343 3820 4955 5636 5827 826 Size: 143 297 477 1135 681 191 1471 Fragments arranged by size: 1471 1414 1135 681 477 297 219 191 143 141 88 84 68 57 6 SfiI GGCCn_nnn'nGGCC Cuts at: 3138 3138 Size: 6472 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 2705 3799 1872 Size: 833 1094 4545 Fragments arranged by size: 4545 1094 833 SmlI C'TyrA_G Cuts at: 820 1017 1943 3187 4576 5477 5739 820 Size: 197 926 1244 1389 901 262 1553 Fragments arranged by size: 1553 1389 1244 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4263 493 Size: 3770 2702 SpeI A'CTAG_T Cuts at: 152 3098 3296 152 Size: 2946 198 3328 Fragments arranged by size: 3328 2946 198 SphI G_CATG'C Cuts at: 1132 3830 1132 Size: 2698 3774 SspI AAT'ATT Cuts at: 5 52 4396 5 Size: 47 4344 2081 Fragments arranged by size: 4344 2081 47 StuI AGG'CCT Cuts at: 1517 3132 1517 Size: 1615 4857 StyI C'CwwG_G Cuts at: 513 1065 2838 3911 513 Size: 552 1773 1073 3074 Fragments arranged by size: 3074 1773 1073 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2047 2073 2980 3137 3141 3472 4499 Size: 1255 467 26 907 157 4 331 1027 Cuts at: 4499 4516 4669 4854 5299 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1027 907 467 445 331 185 157 153 26 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2330 4718 6430 Size: 274 80 33 51 1443 353 2388 1712 Cuts at: 6430 96 Size: 138 Fragments arranged by size: 2388 1712 1443 353 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3597 3629 6432 6464 3597 Size: 32 2803 32 3605 Fragments arranged by size: 3605 2803 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3737 1316 Size: 2421 4051 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2845 3335 2845 Size: 490 5982 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6472 XmaI C'CCGG_G Cuts at: 1870 2703 3797 1870 Size: 833 1094 4545 Fragments arranged by size: 4545 1094 833 XmnI GAAnn'nnTTC Cuts at: 4601 4601 Size: 6472 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2624 4280 276 Size: 53 83 186 936 1090 1656 2468 Fragments arranged by size: 2468 1656 1090 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI