################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EaeI EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AflIII 680 ACATGT A^C[GA][CT]GT AhdI 505 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 334 GGGCCC GGGCC^C AspEI 505 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 957 GGATCC G^GATCC BbvCI 394 GCTGAGG CCTCAGC(-5/-2) BsaBI 934 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 72 TCCGGT [AT]^CCGG[AT] BsgI 162 GTGCAG GTGCAG(16/14) BspLU11I 680 ACATGT A^CATGT BspMI 14 ACCTGC ACCTGC(4/8) BstAPI 104 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 75 GGTCACC G^GT[ACGT]ACC BstPI 75 GGTCACC G^GT[ACGT]ACC BstXI 379 CCAATAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 13 GACCTGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 953 CCACGG C^C[GA][CT]GG Eam1105I 505 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 75 GGTCACC G^GT[ACGT]ACC MaeII 719 ACGT A^CGT MamI 934 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PflMI 963 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Van91I 963 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1049 CCAACTGGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwNI 105 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 592 CAGGCACTG Asp700I 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 877 GAAATGATTC BbsI 22 GTCTTC GAAGAC(2/6) 219 GTCTTC BpmI 730 CTCCAG CTGGAG(16/14) 824 CTGGAG BpuAI 22 GTCTTC GAAGAC(2/6) 219 GTCTTC BsmI 400 GAATGC GAATGC(1/-1) 1000 GCATTC Bsp1407I 683 TGTACA T^GTACA 1342 TGTACA BsrGI 683 TGTACA T^GTACA 1342 TGTACA CfoI 150 GCGC GCG^C 994 GCGC DraII 106 AGGCCCT [GA]G^G[ACGT]CC[CT] 334 GGGCCCT EcoNI 900 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1136 CCTCAAAGAGG EcoO109I 106 AGGCCCT [GA]G^G[ACGT]CC[CT] 334 GGGCCCT HapII 73 CCGG C^CGG 1010 CCGG HhaI 150 GCGC GCG^C 994 GCGC HinP1I 150 GCGC G^CGC 994 GCGC HpaII 73 CCGG C^CGG 1010 CCGG MspI 73 CCGG C^CGG 1010 CCGG NlaIV 334 GGGCCC GG[ACGT]^[ACGT]CC 957 GGATCC PstI 589 CTGCAG CTGCA^G 1085 CTGCAG SapI 613 GAAGAGC GCTCTTC(1/4) 300 GCTCTTC SspBI 683 TGTACA T^GTACA 1342 TGTACA StuI 1201 AGGCCT AGG^CCT 1321 AGGCCT XmnI 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 877 GAAATGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AfaI AluI AlwI AspHI BanII BbvI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NspI PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII