(Circular) (Six-base) MAPSORT of: pFN21AE4086.seq Check: 3284 from: 1 to: 6052 pFN21AE4086 6052 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3862 278 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3395 1937 Size: 1458 4594 Acc65I G'GTAC_C Cuts at: 3373 3373 Size: 6052 AclI AA'CG_TT Cuts at: 1563 4179 4552 1563 Size: 2616 373 3063 Fragments arranged by size: 3063 2616 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2839 4115 5499 5767 1632 Size: 1207 1276 1384 268 1917 Fragments arranged by size: 1917 1384 1276 1207 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5855 AflIII A'CryG_T Cuts at: 1203 2685 4951 5928 1203 Size: 1482 2266 977 1327 Fragments arranged by size: 2266 1482 1327 977 AgeI A'CCGG_T Cuts at: 4883 4883 Size: 6052 AhdI GACnn_n'nnGTC Cuts at: 2515 4781 2515 Size: 2266 3786 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6052 AlwNI CAG_nnn'CTG Cuts at: 2115 2602 3795 5367 2115 Size: 487 1193 1572 2800 Fragments arranged by size: 2800 1572 1193 487 ApaI G_GGCC'C Cuts at: 2343 2343 Size: 6052 ApaLI G'TGCA_C Cuts at: 4109 5265 4109 Size: 1156 4896 ApoI r'AATT_y Cuts at: 1493 3361 3587 3739 5848 1493 Size: 1868 226 152 2109 1697 Fragments arranged by size: 2109 1868 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4606 160 Size: 872 3574 1606 Fragments arranged by size: 3574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 AvaI C'yCGr_G Cuts at: 1870 1943 3377 1870 Size: 73 1434 4545 Fragments arranged by size: 4545 1434 73 BamHI G'GATC_C Cuts at: 2962 3382 3749 2962 Size: 420 367 5265 Fragments arranged by size: 5265 420 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3373 4828 618 Size: 325 217 627 1586 1455 1842 Fragments arranged by size: 1842 1586 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2043 2307 2343 2811 3371 1391 Size: 652 264 36 468 560 4072 Fragments arranged by size: 4072 652 560 468 264 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2020 2217 928 Size: 971 121 197 4763 Fragments arranged by size: 4763 971 197 121 BbvCI CC'TCA_GC Cuts at: 2400 2400 Size: 6052 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4243 4277 1903 Size: 34 2306 34 3678 Fragments arranged by size: 3678 2306 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3944 5154 3944 Size: 1210 4842 BclI T'GATC_A Cuts at: 1548 1548 Size: 6052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4663 136 Size: 107 122 71 631 605 2991 1525 Fragments arranged by size: 2991 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6047 6047 Size: 6052 BlpI GC'TnA_GC Cuts at: 3469 3469 Size: 6052 Bme1580I G_kGCm'C Cuts at: 1790 2100 2343 4113 5269 1790 Size: 310 243 1770 1156 2573 Fragments arranged by size: 2573 1770 1156 310 243 BmgBI CAC'GTC Cuts at: 1318 2725 1318 Size: 1407 4645 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3037 3783 4741 Size: 416 53 83 399 24 1620 746 958 Cuts at: 4741 442 Size: 1753 Fragments arranged by size: 1753 1620 958 746 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6052 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2720 2850 4712 1239 Size: 1481 130 1862 2579 Fragments arranged by size: 2579 1862 1481 130 Bpu10I CC'TnA_GC Cuts at: 2400 2400 Size: 6052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4177 5042 5340 4177 Size: 865 298 4889 Fragments arranged by size: 4889 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4715 882 Size: 617 3216 2219 Fragments arranged by size: 3216 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3843 493 Size: 711 2639 2702 Fragments arranged by size: 2702 2639 711 BsaBI GATnn'nnATC Cuts at: 2943 6046 2943 Size: 3103 2949 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3859 4241 4876 275 Size: 53 83 186 936 2326 382 635 1451 Fragments arranged by size: 2326 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2077 4485 4883 5157 5304 5715 2077 Size: 2408 398 274 147 411 2414 Fragments arranged by size: 2414 2408 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3163 3193 1178 Size: 30 165 30 1760 30 4037 Fragments arranged by size: 4037 1760 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2306 2591 2594 3040 3163 1186 Size: 1120 285 3 446 123 4075 Fragments arranged by size: 4075 1120 446 285 123 3 BseYI C'CCAG_C Cuts at: 2790 5255 5751 2790 Size: 2465 496 3091 Fragments arranged by size: 3091 2465 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2188 1163 Size: 573 452 5027 Fragments arranged by size: 5027 573 452 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3502 4263 5291 1540 Size: 275 186 1501 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2043 2149 2286 2307 2364 2811 2872 2879 2956 Size: 106 137 21 57 447 61 7 77 Cuts at: 2956 4113 4198 5269 2043 Size: 1157 85 1071 2826 Fragments arranged by size: 2826 1157 1071 447 137 106 85 77 61 57 21 7 BsmI GAATG_Cn' Cuts at: 2411 3005 3563 3656 2411 Size: 594 558 93 4807 Fragments arranged by size: 4807 594 558 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2100 2149 2286 2307 2343 2364 Size: 399 253 57 49 137 21 36 21 Cuts at: 2364 2811 2872 2879 2956 3371 4113 4198 5269 Size: 447 61 7 77 415 742 85 1071 Cuts at: 5269 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 447 415 399 253 137 85 77 61 57 49 36 21 21 7 BspEI T'CCGG_A Cuts at: 5715 5715 Size: 6052 BspHI T'CATG_A Cuts at: 3941 5671 3941 Size: 1730 4322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2028 3407 844 Size: 1184 1379 3489 Fragments arranged by size: 3489 1379 1184 BsrBI CCG'CTC Cuts at: 1394 1823 3939 1394 Size: 429 2116 3507 Fragments arranged by size: 3507 2116 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4547 4721 65 Size: 1183 3299 174 1396 Fragments arranged by size: 3299 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4696 4883 1700 Size: 110 2886 187 2869 Fragments arranged by size: 2886 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2688 3347 96 Size: 2592 659 2801 Fragments arranged by size: 2801 2592 659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6052 BssSI C'ACGA_G Cuts at: 4112 5124 4112 Size: 1012 5040 BstAPI GCAn_nnn'nTGC Cuts at: 794 2115 794 Size: 1321 4731 BstBI TT'CG_AA Cuts at: 4856 4856 Size: 6052 BstEII G'GTnAC_C Cuts at: 1184 2080 1184 Size: 896 5156 BstXI CCAn_nnnn'nTGG Cuts at: 2391 2391 Size: 6052 BstYI r'GATC_y Cuts at: 1972 2074 2962 3248 3382 3749 4134 4151 5603 Size: 102 888 286 134 367 385 17 1452 Cuts at: 5603 5689 5701 6047 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 888 385 367 346 286 134 102 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2958 513 Size: 552 939 954 3607 Fragments arranged by size: 3607 954 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5313 Fragments arranged by size: 5313 683 56 BtsI GCAGTG_nn' Cuts at: 3568 4362 4382 3568 Size: 794 20 5238 Fragments arranged by size: 5238 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6052 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6017 DraI TTT'AAA Cuts at: 3356 3356 Size: 6052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2024 5059 809 Size: 507 708 3035 1802 Fragments arranged by size: 3035 1802 708 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3499 4388 6017 Size: 54 1425 50 275 18 1669 889 1629 Cuts at: 6017 8 Size: 43 Fragments arranged by size: 1669 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3499 1537 Size: 275 1687 4090 Fragments arranged by size: 4090 1687 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2312 2522 2613 3319 3982 1353 Size: 959 210 91 706 663 3423 Fragments arranged by size: 3423 959 706 663 210 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4639 5013 5159 387 Size: 1064 138 3050 374 146 1280 Fragments arranged by size: 3050 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2041 2305 2809 2041 Size: 264 504 5284 Fragments arranged by size: 5284 504 264 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2720 2839 2850 4115 4712 5499 5767 Size: 393 1088 119 11 1265 597 787 268 Cuts at: 5767 1239 Size: 1524 Fragments arranged by size: 1524 1265 1088 787 597 393 268 119 11 EcoNI CCTnn'n_nnAGG Cuts at: 2909 3145 2909 Size: 236 5816 EcoO109I rG'GnC_Cy Cuts at: 2112 2340 2112 Size: 228 5824 EcoRI G'AATT_C Cuts at: 3361 3739 3361 Size: 378 5674 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2209 2323 3299 2209 Size: 114 976 4962 Fragments arranged by size: 4962 976 114 FspI TGC'GCA Cuts at: 4558 4558 Size: 6052 HaeII r_GCGC'y Cuts at: 1678 5199 1678 Size: 3521 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2233 2265 2501 2533 3139 Size: 32 135 32 661 32 236 32 606 Cuts at: 3139 3171 3233 3265 4693 4725 4767 4799 1373 Size: 32 62 32 1428 32 42 32 2626 Fragments arranged by size: 2626 1428 661 606 236 135 62 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3396 3642 669 Size: 988 281 1458 246 3079 Fragments arranged by size: 3079 1458 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6052 HpaI GTT'AAC Cuts at: 3642 3642 Size: 6052 KpnI G_GTAC'C Cuts at: 3377 3377 Size: 6052 MfeI C'AATT_G Cuts at: 3651 3651 Size: 6052 MluI A'CGCG_T Cuts at: 5928 5928 Size: 6052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2653 3078 5166 5350 1305 Size: 1348 425 2088 184 2007 Fragments arranged by size: 2088 2007 1348 425 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4230 Fragments arranged by size: 4230 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2882 2949 4010 4369 4528 518 Size: 648 891 825 67 1061 359 159 2042 Fragments arranged by size: 2042 1061 891 825 648 359 159 67 MspA1I CmG'CkG Cuts at: 1606 3468 4145 5293 5538 1606 Size: 1862 677 1148 245 2120 Fragments arranged by size: 2120 1862 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4561 Fragments arranged by size: 4561 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6052 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6052 NotI GC'GGCC_GC Cuts at: 3499 3499 Size: 6052 NspI r_CATG'y Cuts at: 1132 1229 2689 2712 2719 2845 3410 4955 1132 Size: 97 1460 23 7 126 565 1545 2229 Fragments arranged by size: 2229 1545 1460 565 126 97 23 7 PasI CC'CwG_GG Cuts at: 1348 2968 1348 Size: 1620 4432 PciI A'CATG_T Cuts at: 2685 4951 2685 Size: 2266 3786 PflMI CCAn_nnn'nTGG Cuts at: 1108 2974 1108 Size: 1866 4186 PfoI T'CCnGG_A Cuts at: 2736 3083 2736 Size: 347 5705 PmeI GTTT'AAAC Cuts at: 3356 3356 Size: 6052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4121 4153 5634 5666 4121 Size: 32 1481 32 4507 Fragments arranged by size: 4507 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6052 PsiI TTA'TAA Cuts at: 2423 3622 2423 Size: 1199 4853 PspOMI G'GGCC_C Cuts at: 2339 2339 Size: 6052 PstI C_TGCA'G Cuts at: 830 2598 3094 3404 830 Size: 1768 496 310 3478 Fragments arranged by size: 3478 1768 496 310 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6052 SacI G_AGCT'C Cuts at: 2043 2307 2811 2043 Size: 264 504 5284 Fragments arranged by size: 5284 504 264 SalI G'TCGA_C Cuts at: 1936 3394 1936 Size: 1458 4594 SapI GCTCTTCn'nnn_ Cuts at: 2312 2613 2312 Size: 301 5751 SbfI CC_TGCA'GG Cuts at: 3404 3404 Size: 6052 ScaI AGT'ACT Cuts at: 4300 6012 4300 Size: 1712 4340 SfcI C'TryA_G Cuts at: 826 1045 2594 3090 3160 3400 4535 5216 5407 Size: 219 1549 496 70 240 1135 681 191 Cuts at: 5407 826 Size: 1471 Fragments arranged by size: 1549 1471 1135 681 496 240 219 191 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6052 SmaI CCC'GGG Cuts at: 1872 3379 1872 Size: 1507 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4156 5057 5319 820 Size: 197 926 2213 901 262 1553 Fragments arranged by size: 2213 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3843 493 Size: 3350 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6052 SphI G_CATG'C Cuts at: 1132 3410 1132 Size: 2278 3774 SspI AAT'ATT Cuts at: 5 52 3976 5 Size: 47 3924 2081 Fragments arranged by size: 3924 2081 47 StuI AGG'CCT Cuts at: 1517 3208 3328 1517 Size: 1691 120 4241 Fragments arranged by size: 4241 1691 120 StyI C'CwwG_G Cuts at: 513 1065 2004 3491 513 Size: 552 939 1487 3074 Fragments arranged by size: 3074 1487 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4079 4096 4249 4434 4879 325 Size: 1255 2499 17 153 185 445 1498 Fragments arranged by size: 2499 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2688 3347 4298 Size: 274 80 33 51 1443 711 659 951 Cuts at: 4298 6010 96 Size: 1712 138 Fragments arranged by size: 1712 1443 951 711 659 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3163 3195 6012 6044 3163 Size: 32 2817 32 3171 Fragments arranged by size: 3171 2817 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6052 XbaI T'CTAG_A Cuts at: 3388 3388 Size: 6052 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3061 3061 Size: 6052 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6052 XmaI C'CCGG_G Cuts at: 1870 3377 1870 Size: 1507 4545 XmnI GAAnn'nnTTC Cuts at: 2066 2886 4181 2066 Size: 820 1295 3937 Fragments arranged by size: 3937 1295 820 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3860 276 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI