(Linear) (Six-base) MAPSORT of: KIEE4086.seq Check: 6750 from: 1 to: 1347 KIEE4086.orf With 182 enzymes: SgfI * July 3, 2012 18:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 834 1347 Size: 834 513 AflIII A'CryG_T Cuts at: 0 680 1347 Size: 680 667 AhdI GACnn_n'nnGTC Cuts at: 0 510 1347 Size: 510 837 AlwNI CAG_nnn'CTG Cuts at: 0 110 597 1347 Size: 110 487 750 Fragments arranged by size: 750 487 110 ApaI G_GGCC'C Cuts at: 0 338 1347 Size: 338 1009 BamHI G'GATC_C Cuts at: 0 957 1347 Size: 957 390 BanII G_rGCy'C Cuts at: 0 38 302 338 806 1347 Size: 38 264 36 468 541 Fragments arranged by size: 541 468 264 38 36 BbsI GAAGACnn'nnnn_ Cuts at: 0 15 212 1347 Size: 15 197 1135 Fragments arranged by size: 1135 197 15 BbvCI CC'TCA_GC Cuts at: 0 395 1347 Size: 395 952 Bme1580I G_kGCm'C Cuts at: 0 95 338 1347 Size: 95 243 1009 Fragments arranged by size: 1009 243 95 BmgBI CAC'GTC Cuts at: 0 720 1347 Size: 720 627 BmrI ACTGGGnnnn_n' Cuts at: 0 1032 1347 Size: 1032 315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 715 845 1347 Size: 715 130 502 Fragments arranged by size: 715 502 130 Bpu10I CC'TnA_GC Cuts at: 0 395 1347 Size: 395 952 BsaBI GATnn'nnATC Cuts at: 0 938 1347 Size: 938 409 BsaWI w'CCGG_w Cuts at: 0 72 1347 Size: 72 1275 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1158 1188 1347 Size: 1158 30 159 Fragments arranged by size: 1158 159 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 301 586 589 1035 1158 1347 Size: 301 285 3 446 123 189 Fragments arranged by size: 446 301 285 189 123 3 BseYI C'CCAG_C Cuts at: 0 785 1347 Size: 785 562 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 183 1347 Size: 183 1164 BsiHKAI G_wGCw'C Cuts at: 0 38 144 281 302 359 806 867 874 Size: 38 106 137 21 57 447 61 7 Cuts at: 874 951 1347 Size: 77 396 Fragments arranged by size: 447 396 137 106 77 61 57 38 21 7 BsmI GAATG_Cn' Cuts at: 0 406 1000 1347 Size: 406 594 347 Fragments arranged by size: 594 406 347 Bsp1286I G_dGCh'C Cuts at: 0 38 95 144 281 302 338 359 806 Size: 38 57 49 137 21 36 21 447 Cuts at: 806 867 874 951 1347 Size: 61 7 77 396 Fragments arranged by size: 447 396 137 77 61 57 49 38 36 21 21 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 23 1347 Size: 23 1324 BsrGI T'GTAC_A Cuts at: 0 683 1342 1347 Size: 683 659 5 Fragments arranged by size: 683 659 5 BstAPI GCAn_nnn'nTGC Cuts at: 0 110 1347 Size: 110 1237 BstEII G'GTnAC_C Cuts at: 0 75 1347 Size: 75 1272 BstXI CCAn_nnnn'nTGG Cuts at: 0 386 1347 Size: 386 961 BstYI r'GATC_y Cuts at: 0 69 957 1243 1347 Size: 69 888 286 104 Fragments arranged by size: 888 286 104 69 BtgI C'CryG_G Cuts at: 0 953 1347 Size: 953 394 DrdI GACnn_nn'nnGTC Cuts at: 0 19 1347 Size: 19 1328 EarI CTCTTCn'nnn_ Cuts at: 0 307 517 608 1314 1347 Size: 307 210 91 706 33 Fragments arranged by size: 706 307 210 91 33 EcoICRI GAG'CTC Cuts at: 0 36 300 804 1347 Size: 36 264 504 543 Fragments arranged by size: 543 504 264 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 715 834 845 1347 Size: 715 119 11 502 Fragments arranged by size: 715 502 119 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 904 1140 1347 Size: 904 236 207 Fragments arranged by size: 904 236 207 EcoO109I rG'GnC_Cy Cuts at: 0 107 335 1347 Size: 107 228 1012 Fragments arranged by size: 1012 228 107 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 204 318 1294 1347 Size: 204 114 976 53 Fragments arranged by size: 976 204 114 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 228 260 496 528 1134 1166 1228 1260 Size: 228 32 236 32 606 32 62 32 Cuts at: 1260 1347 Size: 87 Fragments arranged by size: 606 236 228 87 62 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 648 1073 1347 Size: 648 425 274 Fragments arranged by size: 648 425 274 MslI CAynn'nnrTG Cuts at: 0 52 877 944 1347 Size: 52 825 67 403 Fragments arranged by size: 825 403 67 52 NspI r_CATG'y Cuts at: 0 684 707 714 840 1347 Size: 684 23 7 126 507 Fragments arranged by size: 684 507 126 23 7 PasI CC'CwG_GG Cuts at: 0 963 1347 Size: 963 384 PciI A'CATG_T Cuts at: 0 680 1347 Size: 680 667 PflMI CCAn_nnn'nTGG Cuts at: 0 969 1347 Size: 969 378 PfoI T'CCnGG_A Cuts at: 0 731 1078 1347 Size: 731 347 269 Fragments arranged by size: 731 347 269 PsiI TTA'TAA Cuts at: 0 418 1347 Size: 418 929 PspOMI G'GGCC_C Cuts at: 0 334 1347 Size: 334 1013 PstI C_TGCA'G Cuts at: 0 593 1089 1347 Size: 593 496 258 Fragments arranged by size: 593 496 258 SacI G_AGCT'C Cuts at: 0 38 302 806 1347 Size: 38 264 504 541 Fragments arranged by size: 541 504 264 38 SapI GCTCTTCn'nnn_ Cuts at: 0 307 608 1347 Size: 307 301 739 Fragments arranged by size: 739 307 301 SfcI C'TryA_G Cuts at: 0 589 1085 1155 1347 Size: 589 496 70 192 Fragments arranged by size: 589 496 192 70 StuI AGG'CCT Cuts at: 0 1203 1323 1347 Size: 1203 120 24 Fragments arranged by size: 1203 120 24 TatI w'GTAC_w Cuts at: 0 683 1342 1347 Size: 683 659 5 Fragments arranged by size: 683 659 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1158 1190 1347 Size: 1158 32 157 Fragments arranged by size: 1158 157 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1056 1347 Size: 1056 291 XmnI GAAnn'nnTTC Cuts at: 0 61 881 1347 Size: 61 820 466 Fragments arranged by size: 820 466 61 Enzymes that do cut: AcuI AflIII AhdI AlwNI ApaI BamHI BanII BbsI BbvCI Bme1580I BmgBI BmrI BpmI Bpu10I BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrGI BstAPI BstEII BstXI BstYI BtgI DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I MmeI MslI NspI PasI PciI PflMI PfoI PsiI PspOMI PstI SacI SapI SfcI StuI TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI BplI BpuEI BsaI BsaAI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EaeI EagI EciI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI