################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII AclI AcsI AgeI AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BlnI BpuAI BsaAI BsaBI BsaI BsiEI BsiWI BspDI BspHI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FseI FspI HinfI HpaI KasI MamI MfeI MluI MluNI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 476 GGTACC G^GTACC AccI 1792 GTAGAC GT^[AC][GT]AC AccIII 443 TCCGGA T^CCGGA AflIII 152 ACGTGT A^C[GA][CT]GT Alw44I 1561 GTGCAC G^TGCAC AocI 1288 CCTGAGG CC^T[ACGT]AGG ApaLI 1561 GTGCAC G^TGCAC Asp718I 476 GGTACC G^GTACC BcgI 1908 CGACAGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 538 CTAG C^TAG BlpI 1417 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1417 GCTCAGC GC^T[ACGT]AGC BseAI 443 TCCGGA T^CCGGA BsmAI 1034 GTCTC GTCTC(1/5) BsmBI 1033 CGTCTC CGTCTC(1/5) BsmI 1009 GAATGC GAATGC(1/-1) Bsp1407I 116 TGTACA T^GTACA BspEI 443 TCCGGA T^CCGGA BsrGI 116 TGTACA T^GTACA BstEII 699 GGTGACC G^GT[ACGT]ACC BstPI 699 GGTGACC G^GT[ACGT]ACC Bsu36I 1288 CCTGAGG CC^T[ACGT]AGG CelII 1417 GCTCAGC GC^T[ACGT]AGC DrdI 43 GACGCACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1198 CGGCCA [CT]^GGCC[GA] Eco81I 1288 CCTGAGG CC^T[ACGT]AGG EcoO65I 699 GGTGACC G^GT[ACGT]ACC HaeII 1039 AGCGCC [GA]GCGC^[CT] HincII 737 GTCAAC GT[CT]^[GA]AC HindII 737 GTCAAC GT[CT]^[GA]AC HindIII 1840 AAGCTT A^AGCTT KpnI 476 GGTACC GGTAC^C KspI 1518 CCGCGG CCGC^GG MaeI 538 CTAG C^TAG MroI 443 TCCGGA T^CCGGA NspI 718 ACATGC [GA]CATG^[CT] PpuMI 212 GGGACCC [GA]G^G[AT]CC[CT] SacII 1518 CCGCGG CCGC^GG SapI 597 GCTCTTC GCTCTTC(1/4) ScaI 1104 AGTACT AGT^ACT SspBI 116 TGTACA T^GTACA StuI 161 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 568 CGCG CG^CG 1519 CGCG AcyI 1822 GGCGTC G[GA]^CG[CT]C 1938 GGCGTC AflII 316 CTTAAG C^TTAAG 1108 CTTAAG AlwI 1047 GATCC GGATC(4/5) 1980 GATCC AvaI 170 CCCGAG C^[CT]CG[GA]G 1169 CCCGAG BcnI 1893 CCCGG CC^[GC]GG 2100 CCGGG BfrI 316 CTTAAG C^TTAAG 1108 CTTAAG BglII 872 AGATCT A^GATCT 1334 AGATCT BsaHI 1822 GGCGTC G[GA]^CG[CT]C 1938 GGCGTC BsaWI 443 TCCGGA [AT]^CCGG[AT] 1058 ACCGGA BsoBI 170 CCCGAG C^[CT]CG[GA]G 1169 CCCGAG BspMI 1371 ACCTGC ACCTGC(4/8) 1565 ACCTGC BsrDI 1225 CATTGC GCAATG(2/0) 581 GCAATG BstUI 568 CGCG CG^CG 1519 CGCG BstXI 1369 CCACCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1964 CCATTGGCATGG DraIII 148 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG 558 CACAGAGTG DsaI 1518 CCGCGG C^C[GA][CT]GG 2130 CCGTGG EarI 598 CTCTTC CTCTTC(1/4) 694 GAAGAG Hin1I 1822 GGCGTC G[GA]^CG[CT]C 1938 GGCGTC Ksp632I 598 CTCTTC CTCTTC(1/4) 694 GAAGAG MvnI 568 CGCG CG^CG 1519 CGCG NaeI 1534 GCCGGC GCC^GGC 2041 GCCGGC NciI 1893 CCCGG CC^[GC]GG 2100 CCGGG NgoMIV 1534 GCCGGC G^CCGGC 2041 GCCGGC PflMI 723 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1430 CCACCCGCTGG TaqI 1235 TCGA T^CGA 1604 TCGA TthHB8I 1235 TCGA T^CGA 1604 TCGA Van91I 723 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1430 CCACCCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaII BanI BanII BbvI BclI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI Tru9I TseI Tsp45I TspRI XhoII