(Linear) (Six-base) MAPSORT of: KIEE4220.seq Check: 6619 from: 1 to: 2277 KIEE4220.orf With 182 enzymes: SgfI * July 3, 2012 18:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1380 1574 2277 Size: 1380 194 703 Fragments arranged by size: 1380 703 194 AccI GT'mk_AC Cuts at: 0 1793 2277 Size: 1793 484 Acc65I G'GTAC_C Cuts at: 0 476 2277 Size: 476 1801 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 979 1199 2277 Size: 979 220 1078 Fragments arranged by size: 1078 979 220 AflII C'TTAA_G Cuts at: 0 316 1108 2277 Size: 316 792 1169 Fragments arranged by size: 1169 792 316 AflIII A'CryG_T Cuts at: 0 152 2277 Size: 152 2125 AleI CACnn'nnGTG Cuts at: 0 784 907 1408 1727 2277 Size: 784 123 501 319 550 Fragments arranged by size: 784 550 501 319 123 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1426 1999 2277 Size: 1426 573 278 Fragments arranged by size: 1426 573 278 AlwNI CAG_nnn'CTG Cuts at: 0 129 1585 1778 2167 2277 Size: 129 1456 193 389 110 Fragments arranged by size: 1456 389 193 129 110 ApaLI G'TGCA_C Cuts at: 0 1561 2277 Size: 1561 716 AvaI C'yCGr_G Cuts at: 0 170 1169 2277 Size: 170 999 1108 Fragments arranged by size: 1108 999 170 BanI G'GyrC_C Cuts at: 0 476 502 613 1462 1861 1915 2277 Size: 476 26 111 849 399 54 362 Fragments arranged by size: 849 476 399 362 111 54 26 BanII G_rGCy'C Cuts at: 0 230 638 1498 2138 2277 Size: 230 408 860 640 139 Fragments arranged by size: 860 640 408 230 139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1897 1931 2277 Size: 1897 34 346 Fragments arranged by size: 1897 346 34 BclI T'GATC_A Cuts at: 0 374 1329 1874 2277 Size: 374 955 545 403 Fragments arranged by size: 955 545 403 374 BglI GCCn_nnn'nGGC Cuts at: 0 191 802 1616 2277 Size: 191 611 814 661 Fragments arranged by size: 814 661 611 191 BglII A'GATC_T Cuts at: 0 872 1334 2277 Size: 872 462 943 Fragments arranged by size: 943 872 462 BlpI GC'TnA_GC Cuts at: 0 1418 2277 Size: 1418 859 Bme1580I G_kGCm'C Cuts at: 0 100 171 391 616 682 773 1465 1473 Size: 100 71 220 225 66 91 692 8 Cuts at: 1473 1565 1866 1907 2057 2149 2176 2230 2277 Size: 92 301 41 150 92 27 54 47 Fragments arranged by size: 692 301 225 220 150 100 92 92 91 71 66 54 47 41 27 8 BmgBI CAC'GTC Cuts at: 0 1239 1246 1752 2277 Size: 1239 7 506 525 Fragments arranged by size: 1239 525 506 7 BmrI ACTGGGnnnn_n' Cuts at: 0 94 1788 2277 Size: 94 1694 489 Fragments arranged by size: 1694 489 94 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 249 2277 Size: 249 2028 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 478 904 991 1171 1852 2197 2277 Size: 478 426 87 180 681 345 80 Fragments arranged by size: 681 478 426 345 180 87 80 Bpu10I CC'TnA_GC Cuts at: 0 1077 2160 2277 Size: 1077 1083 117 Fragments arranged by size: 1083 1077 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 793 1498 2277 Size: 793 705 779 Fragments arranged by size: 793 779 705 BsaHI Gr'CG_yC Cuts at: 0 1823 1939 2277 Size: 1823 116 338 Fragments arranged by size: 1823 338 116 BsaWI w'CCGG_w Cuts at: 0 443 1058 2277 Size: 443 615 1219 Fragments arranged by size: 1219 615 443 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 205 235 922 952 1786 1816 2277 Size: 205 30 687 30 834 30 461 Fragments arranged by size: 834 687 461 205 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 901 946 988 1203 1465 2277 Size: 901 45 42 215 262 812 Fragments arranged by size: 901 812 262 215 45 42 BseYI C'CCAG_C Cuts at: 0 217 390 626 957 1310 1578 1613 1797 Size: 217 173 236 331 353 268 35 184 Cuts at: 1797 1986 2277 Size: 189 291 Fragments arranged by size: 353 331 291 268 236 217 189 184 173 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 540 1138 1552 1576 2031 2277 Size: 540 598 414 24 455 246 Fragments arranged by size: 598 540 455 414 246 24 BsiHKAI G_wGCw'C Cuts at: 0 791 1058 1565 2277 Size: 791 267 507 712 Fragments arranged by size: 791 712 507 267 BsmI GAATG_Cn' Cuts at: 0 1015 2277 Size: 1015 1262 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1039 2277 Size: 1039 1238 Bsp1286I G_dGCh'C Cuts at: 0 100 171 230 391 616 638 682 773 Size: 100 71 59 161 225 22 44 91 Cuts at: 773 791 1058 1465 1473 1498 1565 1866 1907 Size: 18 267 407 8 25 67 301 41 Cuts at: 1907 2057 2138 2149 2176 2230 2277 Size: 150 81 11 27 54 47 Fragments arranged by size: 407 301 267 225 161 150 100 91 81 71 67 59 54 47 44 41 27 25 22 18 11 8 BspEI T'CCGG_A Cuts at: 0 443 2277 Size: 443 1834 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1380 1574 2277 Size: 1380 194 703 Fragments arranged by size: 1380 703 194 BsrBI CCG'CTC Cuts at: 0 423 474 1023 1632 2094 2277 Size: 423 51 549 609 462 183 Fragments arranged by size: 609 549 462 423 183 51 BsrDI GCAATG_nn' Cuts at: 0 588 1224 2277 Size: 588 636 1053 Fragments arranged by size: 1053 636 588 BsrFI r'CCGG_y Cuts at: 0 308 1534 1703 1714 2041 2277 Size: 308 1226 169 11 327 236 Fragments arranged by size: 1226 327 308 236 169 11 BsrGI T'GTAC_A Cuts at: 0 116 2277 Size: 116 2161 BstAPI GCAn_nnn'nTGC Cuts at: 0 563 587 719 948 2277 Size: 563 24 132 229 1329 Fragments arranged by size: 1329 563 229 132 24 BstEII G'GTnAC_C Cuts at: 0 699 2277 Size: 699 1578 BstXI CCAn_nnnn'nTGG Cuts at: 0 1376 1971 2277 Size: 1376 595 306 Fragments arranged by size: 1376 595 306 BstYI r'GATC_y Cuts at: 0 872 1046 1334 1979 2277 Size: 872 174 288 645 298 Fragments arranged by size: 872 645 298 288 174 Bsu36I CC'TnA_GG Cuts at: 0 1289 2277 Size: 1289 988 BtgI C'CryG_G Cuts at: 0 1518 2130 2277 Size: 1518 612 147 Fragments arranged by size: 1518 612 147 BtsI GCAGTG_nn' Cuts at: 0 988 2277 Size: 988 1289 DraIII CAC_nnn'GTG Cuts at: 0 153 563 2277 Size: 153 410 1714 Fragments arranged by size: 1714 410 153 DrdI GACnn_nn'nnGTC Cuts at: 0 49 2277 Size: 49 2228 EaeI y'GGCC_r Cuts at: 0 1198 2277 Size: 1198 1079 EarI CTCTTCn'nnn_ Cuts at: 0 604 689 2277 Size: 604 85 1588 Fragments arranged by size: 1588 604 85 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1210 2277 Size: 1210 1067 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 478 904 979 991 1171 1199 1852 2197 Size: 478 426 75 12 180 28 653 345 Cuts at: 2197 2277 Size: 80 Fragments arranged by size: 653 478 426 345 180 80 75 28 12 EcoO109I rG'GnC_Cy Cuts at: 0 213 669 804 1293 1426 2164 2277 Size: 213 456 135 489 133 738 113 Fragments arranged by size: 738 489 456 213 135 133 113 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 342 2277 Size: 342 1935 HaeII r_GCGC'y Cuts at: 0 1043 2277 Size: 1043 1234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 205 237 491 523 922 954 1480 1512 Size: 205 32 254 32 399 32 526 32 Cuts at: 1512 1786 1818 2277 Size: 274 32 459 Fragments arranged by size: 526 459 399 274 254 205 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 739 2277 Size: 739 1538 HindIII A'AGCT_T Cuts at: 0 1840 2277 Size: 1840 437 KpnI G_GTAC'C Cuts at: 0 480 2277 Size: 480 1797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 684 2277 Size: 684 1593 MslI CAynn'nnrTG Cuts at: 0 784 907 1408 1727 1969 2277 Size: 784 123 501 319 242 308 Fragments arranged by size: 784 501 319 308 242 123 MspA1I CmG'CkG Cuts at: 0 767 945 1152 1204 1436 1443 1520 1613 Size: 767 178 207 52 232 7 77 93 Cuts at: 1613 1901 1913 2277 Size: 288 12 364 Fragments arranged by size: 767 364 288 232 207 178 93 77 52 12 7 NaeI GCC'GGC Cuts at: 0 1536 2043 2277 Size: 1536 507 234 Fragments arranged by size: 1536 507 234 NgoMIV G'CCGG_C Cuts at: 0 1534 2041 2277 Size: 1534 507 236 Fragments arranged by size: 1534 507 236 NspI r_CATG'y Cuts at: 0 722 2277 Size: 722 1555 PasI CC'CwG_GG Cuts at: 0 340 608 1764 2277 Size: 340 268 1156 513 Fragments arranged by size: 1156 513 340 268 PflMI CCAn_nnn'nTGG Cuts at: 0 729 1436 2277 Size: 729 707 841 Fragments arranged by size: 841 729 707 PfoI T'CCnGG_A Cuts at: 0 1049 2111 2213 2277 Size: 1049 1062 102 64 Fragments arranged by size: 1062 1049 102 64 PpuMI rG'GwC_Cy Cuts at: 0 213 2277 Size: 213 2064 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1655 1687 2277 Size: 1655 32 590 Fragments arranged by size: 1655 590 32 PvuII CAG'CTG Cuts at: 0 945 1152 1204 2277 Size: 945 207 52 1073 Fragments arranged by size: 1073 945 207 52 SacII CC_GC'GG Cuts at: 0 1521 2277 Size: 1521 756 SanDI GG'GwC_CC Cuts at: 0 213 2277 Size: 213 2064 SapI GCTCTTCn'nnn_ Cuts at: 0 604 2277 Size: 604 1673 ScaI AGT'ACT Cuts at: 0 1106 2277 Size: 1106 1171 SmlI C'TyrA_G Cuts at: 0 316 808 1108 1477 2277 Size: 316 492 300 369 800 Fragments arranged by size: 800 492 369 316 300 StuI AGG'CCT Cuts at: 0 163 2277 Size: 163 2114 StyI C'CwwG_G Cuts at: 0 399 548 665 926 2277 Size: 399 149 117 261 1351 Fragments arranged by size: 1351 399 261 149 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 631 775 1324 2277 Size: 631 144 549 953 Fragments arranged by size: 953 631 549 144 TatI w'GTAC_w Cuts at: 0 116 1104 2205 2277 Size: 116 988 1101 72 Fragments arranged by size: 1101 988 116 72 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 79 111 339 371 2277 Size: 79 32 228 32 1906 Fragments arranged by size: 1906 228 79 32 32 Enzymes that do cut: AarI AccI Acc65I AcuI AflII AflIII AleI AlfI AlwNI ApaLI AvaI BanI BanII BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII KpnI MmeI MslI MspA1I NaeI NgoMIV NspI PasI PflMI PfoI PpuMI PsrI PvuII SacII SanDI SapI ScaI SmlI StuI StyI TaqII TatI TstI Enzymes that do not cut: AatII AclI AfeI AgeI AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BfrBI BmtI BsaI BsaAI BsaBI BsiEI BsiWI BspHI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HpaI KasI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI SalI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI