################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BamHI BbeI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstUI BstXI BstZ17I Bsu36I CelII CfoI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspI PacI PflFI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 575 GTAGAC GT^[AC][GT]AC AgeI 1521 ACCGGT A^CCGGT Alw44I 1130 GTGCAC G^TGCAC AlwI 1222 GATCC GGATC(4/5) ApaI 1243 GGGCCC GGGCC^C ApaLI 1130 GTGCAC G^TGCAC Asp700I 1972 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 2111 TGGCCA TGG^CCA BbrPI 1211 CACGTG CAC^GTG BbsI 994 GAAGAC GAAGAC(2/6) BcgI 2230 CGATTCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1963 CTAG C^TAG BglII 358 AGATCT A^GATCT BpuAI 994 GAAGAC GAAGAC(2/6) BsaAI 1211 CACGTG [CT]AC^GT[GA] BsaI 1094 GAGACC GGTCTC(1/5) BsaWI 1521 ACCGGT [AT]^CCGG[AT] BspDI 451 ATCGAT AT^CGAT BsrBI 2008 GAGCGG CCGCTC(-3/-3) BsrFI 1521 ACCGGT [GA]^CCGG[CT] BstBI 1484 TTCGAA TT^CGAA BstEII 759 GGTCACC G^GT[ACGT]ACC BstPI 759 GGTCACC G^GT[ACGT]ACC BstYI 358 AGATCT [GA]^GATC[CT] Cfr10I 1521 ACCGGT [GA]^CCGG[CT] ClaI 451 ATCGAT AT^CGAT DraII 1946 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 2111 TGGCCA [CT]^GGCC[GA] EcoO109I 1946 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 759 GGTCACC G^GT[ACGT]ACC EcoRV 1022 GATATC GAT^ATC EcoT22I 548 ATGCAT ATGCA^T HapII 1522 CCGG C^CGG HpaII 1522 CCGG C^CGG MaeI 1963 CTAG C^TAG MflI 358 AGATCT [GA]^GATC[CT] MluNI 2111 TGGCCA TGG^CCA MscI 2111 TGGCCA TGG^CCA MspI 1522 CCGG C^CGG NdeI 968 CATATG CA^TATG NsiI 548 ATGCAT ATGCA^T NspV 1484 TTCGAA TT^CGAA PinAI 1521 ACCGGT A^CCGGT PleI 1104 GACTC GAGTC(4/5) PmaCI 1211 CACGTG CAC^GTG PmlI 1211 CACGTG CAC^GTG PstI 1672 CTGCAG CTGCA^G SacI 1177 GAGCTC GAGCT^C SapI 2089 GAAGAGC GCTCTTC(1/4) ScaI 625 AGTACT AGT^ACT SfuI 1484 TTCGAA TT^CGAA XhoII 358 AGATCT [GA]^GATC[CT] XmnI 1972 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanI 1560 GGTGCC G^G[CT][GA]CC 1686 GGTGCC BsmFI 927 GTCCC GGGAC(10/14) 1447 GTCCC BsmI 546 GAATGC GAATGC(1/-1) 1487 GAATGC DpnII 359 GATC ^GATC 1222 GATC EcoRI 700 GAATTC G^AATTC 1481 GAATTC HincII 229 GTTGAC GT[CT]^[GA]AC 772 GTTGAC HindII 229 GTTGAC GT[CT]^[GA]AC 772 GTTGAC MaeII 1212 ACGT A^CGT 1638 ACGT MboI 359 GATC ^GATC 1222 GATC MspA1I 1831 CAGCTG C[AC]G^C[GT]G 1981 CAGCTG NdeII 359 GATC ^GATC 1222 GATC NlaIII 98 CATG CATG^ 409 CATG PaeR7I 1180 CTCGAG C^TCGAG 1625 CTCGAG PvuII 1831 CAGCTG CAG^CTG 1981 CAGCTG Sau3AI 359 GATC ^GATC 1222 GATC SfcI 1672 CTGCAG C^T[GA][CT]AG 2080 CTACAG SmlI 1180 CTCGAG C^T[CT][GA]AG 1625 CTCGAG XhoI 1180 CTCGAG C^TCGAG 1625 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI AspHI AvaI BanII BbvCI BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboII MnlI MseI MvaI MwoI NlaIV PflMI RsaI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I