Restriction map for
pFN21AE3290
(Circular) (Six-base) MAPSORT of: pFN21AE3290.seq Check: 7717 from: 1 to: 6946
pFN21AE3290 6946 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2614 2614
Size: 6946
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4756 278
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2581 4289 1937
Size: 644 1708 4594
Fragments arranged by size:
4594 1708 644
Acc65I G'GTAC_C
Cuts at: 4267 4267
Size: 6946
AclI AA'CG_TT
Cuts at: 1563 5073 5446 1563
Size: 3510 373 3063
Fragments arranged by size:
3510 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 3833 5009 6393 6661 1632
Size: 554 1647 1176 1384 268 1917
Fragments arranged by size:
1917 1647 1384 1176 554 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6749
AflIII A'CryG_T
Cuts at: 1203 5845 6822 1203
Size: 4642 977 1327
Fragments arranged by size:
4642 1327 977
AgeI A'CCGG_T
Cuts at: 3526 5777 3526
Size: 2251 4695
AhdI GACnn_n'nnGTC
Cuts at: 5675 5675
Size: 6946
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6946
AlwNI CAG_nnn'CTG
Cuts at: 3947 4024 4174 4201 4689 6261 3947
Size: 77 150 27 488 1572 4632
Fragments arranged by size:
4632 1572 488 150 77 27
ApaI G_GGCC'C
Cuts at: 3252 3252
Size: 6946
ApaLI G'TGCA_C
Cuts at: 3135 5003 6159 3135
Size: 1868 1156 3922
Fragments arranged by size:
3922 1868 1156
ApoI r'AATT_y
Cuts at: 1493 2463 2705 3206 3371 3486 4223 4255 4481
Size: 970 242 501 165 115 737 32 226
Cuts at: 4481 4633 6742 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 970 737 501 242 226 165
152 115 32
AseI AT'TA_AT
Cuts at: 160 1032 5500 160
Size: 872 4468 1606
Fragments arranged by size:
4468 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
AvaI C'yCGr_G
Cuts at: 1870 1943 2889 3185 3630 4271 1870
Size: 73 946 296 445 641 4545
Fragments arranged by size:
4545 946 641 445 296 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4148 4181 4148
Size: 33 6913
BamHI G'GATC_C
Cuts at: 4276 4643 4276
Size: 367 6579
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3565 3691 4267 5722 618
Size: 325 217 627 1778 126 576 1455 1842
Fragments arranged by size:
1842 1778 1455 627 576 325 217 126
BanII G_rGCy'C
Cuts at: 1391 3186 3252 3915 4265 1391
Size: 1795 66 663 350 4072
Fragments arranged by size:
4072 1795 663 350 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3006 928
Size: 971 1107 4868
Fragments arranged by size:
4868 1107 971
BbvCI CC'TCA_GC
Cuts at: 3177 3948 4041 3177
Size: 771 93 6082
Fragments arranged by size:
6082 771 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4224 4258 5137 5171 1903
Size: 34 2287 34 879 34 3678
Fragments arranged by size:
3678 2287 879 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4838 6048 4838
Size: 1210 5736
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6946
BfrBI ATG'CAT
Cuts at: 2555 2555
Size: 6946
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5557 136
Size: 107 122 71 631 605 3885 1525
Fragments arranged by size:
3885 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2363 6941 2363
Size: 4578 2368
BlpI GC'TnA_GC
Cuts at: 4363 4363
Size: 6946
Bme1580I G_kGCm'C
Cuts at: 1790 2982 3139 3252 3696 5007 6163 1790
Size: 1192 157 113 444 1311 1156 2573
Fragments arranged by size:
2573 1311 1192 1156 444 157 113
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6946
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2150 4677 5635
Size: 416 53 83 399 24 733 2527 958
Cuts at: 5635 442
Size: 1753
Fragments arranged by size:
2527 1753 958 733 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6946
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2382 2382
Size: 6946
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2178 2328 2669 3191 4046 4196 5606 1239
Size: 939 150 341 522 855 150 1410 2579
Fragments arranged by size:
2579 1410 939 855 522 341 150 150
Bpu10I CC'TnA_GC
Cuts at: 3177 3948 4041 3177
Size: 771 93 6082
Fragments arranged by size:
6082 771 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5071 5936 6234 5071
Size: 865 298 5783
Fragments arranged by size:
5783 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3093 5609 882
Size: 617 1594 2516 2219
Fragments arranged by size:
2516 2219 1594 617
BsaAI yAC'GTr
Cuts at: 493 1204 3218 4737 493
Size: 711 2014 1519 2702
Fragments arranged by size:
2702 2014 1519 711
BsaBI GATnn'nnATC
Cuts at: 6940 6940
Size: 6946
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4753 5135 5770 275
Size: 53 83 186 936 3220 382 635 1451
Fragments arranged by size:
3220 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3526 5379 5777 6051 6198 6609 3526
Size: 1853 398 274 147 411 3863
Fragments arranged by size:
3863 1853 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 4169 4199 1178
Size: 30 165 30 717 30 2019 30 3925
Fragments arranged by size:
3925 2019 717 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2651 3052 3194 4088 4124 1186
Size: 940 525 401 142 894 36 4008
Fragments arranged by size:
4008 940 894 525 401 142 36
BseYI C'CCAG_C
Cuts at: 2349 4062 6149 6645 2349
Size: 1713 2087 496 2650
Fragments arranged by size:
2650 2087 1713 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6373
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4396 5157 6185 1540
Size: 275 186 2395 761 1028 2301
Fragments arranged by size:
2395 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2530 3139 3186 3811 5007 5092 6163 2530
Size: 609 47 625 1196 85 1071 3313
Fragments arranged by size:
3313 1196 1071 625 609 85 47
BsmI GAATG_Cn'
Cuts at: 2557 3498 4457 4550 2557
Size: 941 959 93 4953
Fragments arranged by size:
4953 959 941 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2530 2982 3139 3186 3252 3696 3811
Size: 399 740 452 157 47 66 444 115
Cuts at: 3811 3915 4265 5007 5092 6163 1391
Size: 104 350 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 740 452 444 399 350
157 115 104 85 66 47
BspEI T'CCGG_A
Cuts at: 6609 6609
Size: 6946
BspHI T'CATG_A
Cuts at: 4835 6565 4835
Size: 1730 5216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2614 3158 3747 4301 844
Size: 1770 544 589 554 3489
Fragments arranged by size:
3489 1770 589 554 544
BsrBI CCG'CTC
Cuts at: 1394 1823 4015 4833 1394
Size: 429 2192 818 3507
Fragments arranged by size:
3507 2192 818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5441 5615 65
Size: 1183 4193 174 1396
Fragments arranged by size:
4193 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3526 5590 5777 1700
Size: 110 1716 2064 187 2869
Fragments arranged by size:
2869 2064 1716 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6946
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6946
BssSI C'ACGA_G
Cuts at: 5006 6018 5006
Size: 1012 5934
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6946
BstBI TT'CG_AA
Cuts at: 3490 5750 3490
Size: 2260 4686
BstEII G'GTnAC_C
Cuts at: 1184 2764 1184
Size: 1580 5366
BstYI r'GATC_y
Cuts at: 1972 2363 4276 4643 5028 5045 6497 6583 6595
Size: 391 1913 367 385 17 1452 86 12
Cuts at: 6595 6941 1972
Size: 346 1977
Fragments arranged by size:
1977 1913 1452 391 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6394
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6207
Fragments arranged by size:
6207 683 56
BtsI GCAGTG_nn'
Cuts at: 2530 4240 4462 5256 5276 2530
Size: 1710 222 794 20 4200
Fragments arranged by size:
4200 1710 794 222 20
ClaI AT'CG_AT
Cuts at: 1554 2457 1554
Size: 903 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3582 3617 529
Size: 35 3018 35 3858
Fragments arranged by size:
3858 3018 35 35
DraI TTT'AAA
Cuts at: 4250 4250
Size: 6946
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5953 809
Size: 507 4637 1802
Fragments arranged by size:
4637 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4116 4393 5282
Size: 54 1425 50 275 18 2286 277 889
Cuts at: 5282 6911 8
Size: 1629 43
Fragments arranged by size:
2286 1629 1425 889 277 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4393 1537
Size: 275 2581 4090
Fragments arranged by size:
4090 2581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2648 3510 3796 3995 4025 4089 4876 1353
Size: 1295 862 286 199 30 64 787 3423
Fragments arranged by size:
3423 1295 862 787 286 199 64 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5533 5907 6053 387
Size: 1064 138 3944 374 146 1280
Fragments arranged by size:
3944 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3184 3184
Size: 6946
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2178 2186 2328 2669 3191 3833 4046
Size: 393 546 8 142 341 522 642 213
Cuts at: 4046 4196 5009 5606 6393 6661 1239
Size: 150 813 597 787 268 1524
Fragments arranged by size:
1524 813 787 642 597 546 522 393
341 268 213 150 142 8
EcoO109I rG'GnC_Cy
Cuts at: 3952 3952
Size: 6946
EcoRI G'AATT_C
Cuts at: 2705 3486 4255 4633 2705
Size: 781 769 378 5018
Fragments arranged by size:
5018 781 769 378
EcoRV GAT'ATC
Cuts at: 3029 3029
Size: 6946
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2288 2288
Size: 6946
FspI TGC'GCA
Cuts at: 5452 5452
Size: 6946
HaeII r_GCGC'y
Cuts at: 1678 6093 1678
Size: 4415 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 3323 3355 3526
Size: 32 135 32 455 32 1264 32 171
Cuts at: 3526 3558 3923 3955 4169 4201 5587 5619 5661
Size: 32 365 32 214 32 1386 32 42
Cuts at: 5661 5693 1373
Size: 32 2626
Fragments arranged by size:
2626 1386 1264 455 365 214 171 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 2779 4290 4536 669
Size: 988 281 298 543 1511 246 3079
Fragments arranged by size:
3079 1511 988 543 298 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6946
HpaI GTT'AAC
Cuts at: 4536 4536
Size: 6946
KpnI G_GTAC'C
Cuts at: 4271 4271
Size: 6946
MfeI C'AATT_G
Cuts at: 4545 4545
Size: 6946
MluI A'CGCG_T
Cuts at: 6822 6822
Size: 6946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4050 6060 6244 1305
Size: 2745 2010 184 2007
Fragments arranged by size:
2745 2010 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4118 10
Size: 54 1425 343 2286 2838
Fragments arranged by size:
2838 2286 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4904 5263 5422 518
Size: 648 3738 359 159 2042
Fragments arranged by size:
3738 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3838 3988 4362 5039 6187 6432 1606
Size: 2232 150 374 677 1148 245 2120
Fragments arranged by size:
2232 2120 1148 677 374 245 150
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6394
NdeI CA'TA_TG
Cuts at: 387 2974 387
Size: 2587 4359
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6946
NotI GC'GGCC_GC
Cuts at: 4393 4393
Size: 6946
NsiI A_TGCA'T
Cuts at: 2557 2557
Size: 6946
NspI r_CATG'y
Cuts at: 1132 1229 4304 5849 1132
Size: 97 3075 1545 2229
Fragments arranged by size:
3075 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6946
PciI A'CATG_T
Cuts at: 5845 5845
Size: 6946
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3560 4069 4174 1108
Size: 2452 509 105 3880
Fragments arranged by size:
3880 2452 509 105
PfoI T'CCnGG_A
Cuts at: 3327 3327
Size: 6946
PmeI GTTT'AAAC
Cuts at: 4250 4250
Size: 6946
PmlI CAC'GTG
Cuts at: 3218 3218
Size: 6946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5015 5047 6528 6560 5015
Size: 32 1481 32 5401
Fragments arranged by size:
5401 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6946
PsiI TTA'TAA
Cuts at: 4516 4516
Size: 6946
PspOMI G'GGCC_C
Cuts at: 3248 3248
Size: 6946
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3633 3665 3633
Size: 32 6914
PstI C_TGCA'G
Cuts at: 830 3681 4298 830
Size: 2851 617 3478
Fragments arranged by size:
3478 2851 617
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6946
PvuII CAG'CTG
Cuts at: 3838 3988 3838
Size: 150 6796
SacI G_AGCT'C
Cuts at: 3186 3186
Size: 6946
SalI G'TCGA_C
Cuts at: 1936 4288 1936
Size: 2352 4594
SapI GCTCTTCn'nnn_
Cuts at: 4089 4089
Size: 6946
SbfI CC_TGCA'GG
Cuts at: 4298 4298
Size: 6946
ScaI AGT'ACT
Cuts at: 2632 5194 6906 2632
Size: 2562 1712 2672
Fragments arranged by size:
2672 2562 1712
SfcI C'TryA_G
Cuts at: 826 1045 3677 4085 4294 5429 6110 6301 826
Size: 219 2632 408 209 1135 681 191 1471
Fragments arranged by size:
2632 1471 1135 681 408 219 209 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6946
SmaI CCC'GGG
Cuts at: 1872 4273 1872
Size: 2401 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3185 3630 5050 5951 6213 820
Size: 197 926 1242 445 1420 901 262 1553
Fragments arranged by size:
1553 1420 1242 926 901 445 262 197
SnaBI TAC'GTA
Cuts at: 493 4737 493
Size: 4244 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6946
SphI G_CATG'C
Cuts at: 1132 4304 1132
Size: 3172 3774
SspI AAT'ATT
Cuts at: 5 52 4870 5
Size: 47 4818 2081
Fragments arranged by size:
4818 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6946
StyI C'CwwG_G
Cuts at: 513 1065 2067 2574 2935 4385 513
Size: 552 1002 507 361 1450 3074
Fragments arranged by size:
3074 1450 1002 552 507 361
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3298 3407 4973 4990 5143 5328 5773
Size: 1255 1718 109 1566 17 153 185 445
Cuts at: 5773 325
Size: 1498
Fragments arranged by size:
1718 1566 1498 1255 445 185 153 109
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2630 3115 3158
Size: 274 80 33 51 1443 653 485 43
Cuts at: 3158 5192 6904 96
Size: 2034 1712 138
Fragments arranged by size:
2034 1712 1443 653 485 274 138 80
51 43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6906 6938 6906
Size: 32 6914
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6946
XbaI T'CTAG_A
Cuts at: 4282 4282
Size: 6946
XhoI C'TCGA_G
Cuts at: 1943 3185 3630 1943
Size: 1242 445 5259
Fragments arranged by size:
5259 1242 445
XmaI C'CCGG_G
Cuts at: 1870 4271 1870
Size: 2401 4545
XmnI GAAnn'nnTTC
Cuts at: 3981 5075 3981
Size: 1094 5852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4754 276
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PpuMI PspXI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI