################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII AccII AccIII AciI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AvaI AviII AvrII BalI BamHI BbeI BbrPI BciVI BcnI BfrI BglII BlnI BsaI BsaWI BseAI BsiEI BsiWI BsmI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstYI BstZ17I Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoNI EcoT22I FauI FseI FspI HapII HpaII KasI KspI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacI SacII SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 1673 GGTACC G^GTACC AclI 1006 AACGTT AA^CGTT AcyI 1971 GACGCC G[GA]^CG[CT]C AhdI 3348 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 3047 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 479 CCTTAGG CC^T[ACGT]AGG Asp718I 1673 GGTACC G^GTACC AspEI 3348 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1766 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 466 GGGCTC G[GA]GC[CT]^C BbvCI 534 GCTGAGG CCTCAGC(-5/-2) BcgI 2798 CGATAGAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 730 GCCATTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2782 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2782 GCTGAGC GC^T[ACGT]AGC BsaAI 838 TACGTG [CT]AC^GT[GA] BsaHI 1971 GACGCC G[GA]^CG[CT]C BseRI 488 CTCCTC GAGGAG(10/8) BspHI 1877 TCATGA T^CATGA BspMI 2320 GCAGGT ACCTGC(4/8) Bsu36I 479 CCTTAGG CC^T[ACGT]AGG CelII 2782 GCTGAGC GC^T[ACGT]AGC CfoI 3139 GCGC GCG^C DraII 483 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 341 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 788 CCATGG C^C[GA][CT]GG EaeI 1548 TGGCCG [CT]^GGCC[GA] Eam1105I 3348 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 479 CCTTAGG CC^T[ACGT]AGG EcoO109I 483 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 2439 GATATC GAT^ATC HaeII 3138 GGCGCT [GA]GCGC^[CT] HhaI 3139 GCGC GCG^C Hin1I 1971 GACGCC G[GA]^CG[CT]C HinP1I 3139 GCGC G^CGC HpaI 1461 GTTAAC GTT^AAC KpnI 1673 GGTACC GGTAC^C NcoI 788 CCATGG C^CATGG NdeI 905 CATATG CA^TATG PflFI 1766 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 483 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 1006 AACGTT AA^CGTT PstI 2420 CTGCAG CTGCA^G RcaI 1877 TCATGA T^CATGA SphI 2986 GCATGC GCATG^C StuI 619 AGGCCT AGG^CCT Tth111I 1766 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2503 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AflIII 1032 ACATGT A^C[GA][CT]GT 2373 ACATGT AlwI 1387 GATCC GGATC(4/5) 529 GGATC AseI 3177 ATTAAT AT^TAAT 3207 ATTAAT AsnI 3177 ATTAAT AT^TAAT 3207 ATTAAT AvaII 484 GGACC G^G[AT]CC 497 GGTCC BanI 1673 GGTACC G^G[CT][GA]CC 2480 GGTGCC BpmI 1859 CTCCAG CTGGAG(16/14) 2177 CTGGAG BsaBI 1615 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2668 GATGAACATC BsmBI 1969 GAGACG CGTCTC(1/5) 2981 GAGACG Bsp1407I 1890 TGTACA T^GTACA 2754 TGTACA BspLU11I 1032 ACATGT A^CATGT 2373 ACATGT BsrDI 2787 GCAATG GCAATG(2/0) 2939 GCAATG BsrGI 1890 TGTACA T^GTACA 2754 TGTACA BstEII 807 GGTTACC G^GT[ACGT]ACC 1260 GGTTACC BstPI 807 GGTTACC G^GT[ACGT]ACC 1260 GGTTACC BstXI 510 CCATCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2104 CCATTGGAGTGG Cfr13I 484 GGACC G^G[ACGT]CC 497 GGTCC EcoO65I 807 GGTTACC G^GT[ACGT]ACC 1260 GGTTACC EcoRI 441 GAATTC G^AATTC 814 GAATTC EcoT14I 788 CCATGG C^C[AT][AT]GG 2078 CCTTGG HgaI 1971 GACGC GACGC(5/10) 2983 GACGC HindIII 100 AAGCTT A^AGCTT 1555 AAGCTT MamI 1615 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2668 GATGAACATC MspA1I 2780 CAGCTG C[AC]G^C[GT]G 2918 CAGCTG PflMI 1624 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2971 CCAAGAATTGG PleI 1857 GACTC GAGTC(4/5) 115 GAGTC PshBI 3177 ATTAAT AT^TAAT 3207 ATTAAT PvuII 2780 CAGCTG CAG^CTG 2918 CAGCTG SalI 1237 GTCGAC G^TCGAC 2954 GTCGAC SapI 468 GCTCTTC GCTCTTC(1/4) 3197 GCTCTTC Sau96I 484 GGACC G^G[ACGT]CC 497 GGTCC ScaI 1217 AGTACT AGT^ACT 1586 AGTACT SmlI 646 CTTGAG C^T[CT][GA]AG 778 CTCAAG SspBI 1890 TGTACA T^GTACA 2754 TGTACA SspI 224 AATATT AAT^ATT 379 AATATT StyI 788 CCATGG C^C[AT][AT]GG 2078 CCTTGG Van91I 1624 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2971 CCAAGAATTGG XcmI 218 CCAAACAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2069 CCAGTGTTCCCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccI AcsI AfaI AluI ApoI Asp700I AspHI BbsI BbvI BclI BfaI BmyI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI Cac8I DdeI DpnII DraI EarI EcoRII FbaI Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI