(Circular) (Six-base) MAPSORT of: pFN21AE4273.seq Check: 5777 from: 1 to: 8272 pFN21AE4273 8272 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4316 4316 Size: 8272 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6082 278 Size: 53 83 186 936 4546 2468 Fragments arranged by size: 4546 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2776 3243 3438 4960 5615 1937 Size: 839 467 195 1522 655 4594 Fragments arranged by size: 4594 1522 839 655 467 195 Acc65I G'GTAC_C Cuts at: 3678 5593 3678 Size: 1915 6357 AclI AA'CG_TT Cuts at: 1563 3012 6399 6772 1563 Size: 1449 3387 373 3063 Fragments arranged by size: 3387 3063 1449 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2844 3417 3557 3729 4548 4767 6335 7719 Size: 1212 573 140 172 819 219 1568 1384 Cuts at: 7719 7987 1632 Size: 268 1917 Fragments arranged by size: 1917 1568 1384 1212 819 573 268 219 172 140 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8075 AflIII A'CryG_T Cuts at: 1203 3037 4378 7171 8148 1203 Size: 1834 1341 2793 977 1327 Fragments arranged by size: 2793 1834 1341 1327 977 AgeI A'CCGG_T Cuts at: 7103 7103 Size: 8272 AhdI GACnn_n'nnGTC Cuts at: 5358 7001 5358 Size: 1643 6629 AleI CACnn'nnGTG Cuts at: 1166 5060 1166 Size: 3894 4378 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4276 1184 Size: 3092 5180 AlwNI CAG_nnn'CTG Cuts at: 5057 6015 7587 5057 Size: 958 1572 5742 Fragments arranged by size: 5742 1572 958 ApaLI G'TGCA_C Cuts at: 6329 7485 6329 Size: 1156 7116 ApoI r'AATT_y Cuts at: 1493 2446 2638 2819 3087 3295 3855 4187 5233 Size: 953 192 181 268 208 560 332 1046 Cuts at: 5233 5513 5581 5807 5959 8068 1493 Size: 280 68 226 152 2109 1697 Fragments arranged by size: 2109 1697 1046 953 560 332 280 268 226 208 192 181 152 68 AseI AT'TA_AT Cuts at: 160 1032 5183 5213 6826 160 Size: 872 4151 30 1613 1606 Fragments arranged by size: 4151 1613 1606 872 30 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8272 AvaI C'yCGr_G Cuts at: 1870 1943 5597 1870 Size: 73 3654 4545 Fragments arranged by size: 4545 3654 73 BamHI G'GATC_C Cuts at: 5602 5969 5602 Size: 367 7905 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3678 4485 5593 7048 618 Size: 325 217 627 1891 807 1108 1455 1842 Fragments arranged by size: 1891 1842 1455 1108 807 627 325 217 BanII G_rGCy'C Cuts at: 1391 2475 5591 1391 Size: 1084 3116 4072 Fragments arranged by size: 4072 3116 1084 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2253 3531 4805 928 Size: 971 354 1278 1274 4395 Fragments arranged by size: 4395 1278 1274 971 354 BbvCI CC'TCA_GC Cuts at: 2540 2540 Size: 8272 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4792 4826 6463 6497 1903 Size: 34 2855 34 1637 34 3678 Fragments arranged by size: 3678 2855 1637 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6164 7374 6164 Size: 1210 7062 BclI T'GATC_A Cuts at: 1548 2980 3598 3670 1548 Size: 1432 618 72 6150 Fragments arranged by size: 6150 1432 618 72 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2741 6883 136 Size: 107 122 71 631 605 1069 4142 1525 Fragments arranged by size: 4142 1525 1069 631 605 122 107 71 BglII A'GATC_T Cuts at: 8267 8267 Size: 8272 BlpI GC'TnA_GC Cuts at: 4788 5689 4788 Size: 901 7371 Bme1580I G_kGCm'C Cuts at: 1790 6333 7489 1790 Size: 4543 1156 2573 Fragments arranged by size: 4543 2573 1156 BmgBI CAC'GTC Cuts at: 1318 4063 1318 Size: 2745 5527 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3345 4050 6003 Size: 416 53 83 399 24 1928 705 1953 Cuts at: 6003 6961 442 Size: 958 1753 Fragments arranged by size: 1953 1928 1753 958 705 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8272 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2565 4637 2565 Size: 2072 6200 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3849 4203 6932 1239 Size: 2610 354 2729 2579 Fragments arranged by size: 2729 2610 2579 354 Bpu10I CC'TnA_GC Cuts at: 2540 3584 2540 Size: 1044 7228 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2672 2768 6397 7262 7560 2672 Size: 96 3629 865 298 3384 Fragments arranged by size: 3629 3384 865 298 96 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6935 882 Size: 617 5436 2219 Fragments arranged by size: 5436 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2845 6063 493 Size: 711 1641 3218 2702 Fragments arranged by size: 3218 2702 1641 711 BsaBI GATnn'nnATC Cuts at: 3624 4677 8266 3624 Size: 1053 3589 3630 Fragments arranged by size: 3630 3589 1053 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3977 6079 6461 7096 Size: 53 83 186 936 2444 2102 382 635 Cuts at: 7096 275 Size: 1451 Fragments arranged by size: 2444 2102 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6705 7103 7377 7524 7935 6705 Size: 398 274 147 411 7042 Fragments arranged by size: 7042 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2277 2307 2558 2588 1178 Size: 30 165 30 874 30 251 30 6862 Fragments arranged by size: 6862 874 251 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2484 1186 Size: 1298 6974 BseYI C'CCAG_C Cuts at: 3160 4562 7475 7971 3160 Size: 1402 2913 496 3461 Fragments arranged by size: 3461 2913 1402 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2589 2641 2867 3041 5052 5419 1163 Size: 573 853 52 226 174 2011 367 4016 Fragments arranged by size: 4016 2011 853 573 367 226 174 52 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5722 6483 7511 1540 Size: 275 186 3721 761 1028 2301 Fragments arranged by size: 3721 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2549 3655 5073 6333 6418 7489 2549 Size: 1106 1418 1260 85 1071 3332 Fragments arranged by size: 3332 1418 1260 1106 1071 85 BsmI GAATG_Cn' Cuts at: 5783 5876 5783 Size: 93 8179 BsmBI CGTCTCn'nnnn_ Cuts at: 3968 4980 3968 Size: 1012 7260 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2475 2549 3655 5073 5591 6333 6418 Size: 399 685 74 1106 1418 518 742 85 Cuts at: 6418 7489 1391 Size: 1071 2174 Fragments arranged by size: 2174 1418 1106 1071 742 685 518 399 85 74 BspEI T'CCGG_A Cuts at: 7935 7935 Size: 8272 BspHI T'CATG_A Cuts at: 3882 6161 7891 3882 Size: 2279 1730 4263 Fragments arranged by size: 4263 2279 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4316 5627 844 Size: 3472 1311 3489 Fragments arranged by size: 3489 3472 1311 BsrBI CCG'CTC Cuts at: 1394 1823 6159 1394 Size: 429 4336 3507 Fragments arranged by size: 4336 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4799 4951 6767 6941 65 Size: 1183 3551 152 1816 174 1396 Fragments arranged by size: 3551 1816 1396 1183 174 152 BsrFI r'CCGG_y Cuts at: 1700 1810 6916 7103 1700 Size: 110 5106 187 2869 Fragments arranged by size: 5106 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3895 4759 96 Size: 3799 864 3609 Fragments arranged by size: 3799 3609 864 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8272 BssSI C'ACGA_G Cuts at: 6332 7344 6332 Size: 1012 7260 BstAPI GCAn_nnn'nTGC Cuts at: 794 2619 4019 4997 794 Size: 1825 1400 978 4069 Fragments arranged by size: 4069 1825 1400 978 BstBI TT'CG_AA Cuts at: 7076 7076 Size: 8272 BstEII G'GTnAC_C Cuts at: 1184 2812 3265 1184 Size: 1628 453 6191 Fragments arranged by size: 6191 1628 453 BstXI CCAn_nnnn'nTGG Cuts at: 2522 4116 2522 Size: 1594 6678 BstYI r'GATC_y Cuts at: 1972 5602 5969 6354 6371 7823 7909 7921 8267 Size: 3630 367 385 17 1452 86 12 346 Cuts at: 8267 1972 Size: 1977 Fragments arranged by size: 3630 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2485 2485 Size: 8272 BtgI C'CryG_G Cuts at: 513 1065 2793 513 Size: 552 1728 5992 Fragments arranged by size: 5992 1728 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2501 487 Size: 683 56 1275 6258 Fragments arranged by size: 6258 1275 683 56 BtsI GCAGTG_nn' Cuts at: 3187 4258 5788 6582 6602 3187 Size: 1071 1530 794 20 4857 Fragments arranged by size: 4857 1530 1071 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8272 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2664 2699 529 Size: 35 2100 35 6102 Fragments arranged by size: 6102 2100 35 35 DraI TTT'AAA Cuts at: 4221 4461 4689 5400 5576 4221 Size: 240 228 711 176 6917 Fragments arranged by size: 6917 711 240 228 176 DraIII CAC_nnn'GTG Cuts at: 2351 2351 Size: 8272 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7279 809 Size: 507 5963 1802 Fragments arranged by size: 5963 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3553 5719 6608 Size: 54 1425 50 275 18 1723 2166 889 Cuts at: 6608 8237 8 Size: 1629 43 Fragments arranged by size: 2166 1723 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5719 1537 Size: 275 3907 4090 Fragments arranged by size: 4090 3907 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2480 2502 2587 3249 4786 5209 5486 6202 Size: 1127 22 85 662 1537 423 277 716 Cuts at: 6202 1353 Size: 3423 Fragments arranged by size: 3423 1537 1127 716 662 423 277 85 22 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6859 7233 7379 387 Size: 1064 138 5270 374 146 1280 Fragments arranged by size: 5270 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2844 3417 3557 3729 3849 4203 4548 Size: 393 1212 573 140 172 120 354 345 Cuts at: 4548 4767 6335 6932 7719 7987 1239 Size: 219 1568 597 787 268 1524 Fragments arranged by size: 1568 1524 1212 787 597 573 393 354 345 268 219 172 140 120 EcoO109I rG'GnC_Cy Cuts at: 2489 2489 Size: 8272 EcoRI G'AATT_C Cuts at: 2446 2819 5581 5959 2446 Size: 373 2762 378 4759 Fragments arranged by size: 4759 2762 378 373 EcoRV GAT'ATC Cuts at: 4446 4446 Size: 8272 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4381 4381 Size: 8272 FspI TGC'GCA Cuts at: 6778 6778 Size: 8272 HaeII r_GCGC'y Cuts at: 1678 5147 7419 1678 Size: 3469 2272 2531 Fragments arranged by size: 3469 2531 2272 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2645 2677 2692 2724 3760 Size: 32 135 32 1073 32 15 32 1036 Cuts at: 3760 3792 4162 4194 4490 4522 5344 5376 6913 Size: 32 370 32 296 32 822 32 1537 Cuts at: 6913 6945 6987 7019 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1537 1073 1036 822 370 296 135 42 32 32 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 3244 3468 3539 4141 4920 4961 Size: 988 281 1306 224 71 602 779 41 Cuts at: 4961 5616 5862 669 Size: 655 246 3079 Fragments arranged by size: 3079 1306 988 779 655 602 281 246 224 71 41 HindIII A'AGCT_T Cuts at: 748 2105 3560 748 Size: 1357 1455 5460 Fragments arranged by size: 5460 1455 1357 HpaI GTT'AAC Cuts at: 3468 5862 3468 Size: 2394 5878 KpnI G_GTAC'C Cuts at: 3682 5597 3682 Size: 1915 6357 MfeI C'AATT_G Cuts at: 5871 5871 Size: 8272 MluI A'CGCG_T Cuts at: 8148 8148 Size: 8272 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3385 3706 3777 3915 7386 7570 1305 Size: 2080 321 71 138 3471 184 2007 Fragments arranged by size: 3471 2080 2007 321 184 138 71 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6450 Fragments arranged by size: 6450 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2520 3987 4114 5060 6230 6589 6748 Size: 648 1354 1467 127 946 1170 359 159 Cuts at: 6748 518 Size: 2042 Fragments arranged by size: 2042 1467 1354 1170 946 648 359 159 127 MspA1I CmG'CkG Cuts at: 1606 4787 4925 5688 6365 7513 7758 1606 Size: 3181 138 763 677 1148 245 2120 Fragments arranged by size: 3181 2120 1148 763 677 245 138 NcoI C'CATG_G Cuts at: 513 1065 2793 513 Size: 552 1728 5992 Fragments arranged by size: 5992 1728 552 NdeI CA'TA_TG Cuts at: 387 2911 387 Size: 2524 5748 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8272 NotI GC'GGCC_GC Cuts at: 5719 5719 Size: 8272 NspI r_CATG'y Cuts at: 1132 1229 2586 3041 4382 4918 4995 5630 7175 Size: 97 1357 455 1341 536 77 635 1545 Cuts at: 7175 1132 Size: 2229 Fragments arranged by size: 2229 1545 1357 1341 635 536 455 97 77 PasI CC'CwG_GG Cuts at: 1348 2831 1348 Size: 1483 6789 PciI A'CATG_T Cuts at: 3037 4378 7171 3037 Size: 1341 2793 4138 Fragments arranged by size: 4138 2793 1341 PflMI CCAn_nnn'nTGG Cuts at: 1108 3635 4982 1108 Size: 2527 1347 4398 Fragments arranged by size: 4398 2527 1347 PfoI T'CCnGG_A Cuts at: 2134 4180 2134 Size: 2046 6226 PmeI GTTT'AAAC Cuts at: 5576 5576 Size: 8272 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2873 2905 6341 6373 7854 7886 2873 Size: 32 3436 32 1481 32 3259 Fragments arranged by size: 3436 3259 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2489 2489 Size: 8272 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8272 PsiI TTA'TAA Cuts at: 5842 5842 Size: 8272 PstI C_TGCA'G Cuts at: 830 4429 5624 830 Size: 3599 1195 3478 Fragments arranged by size: 3599 3478 1195 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8272 PvuII CAG'CTG Cuts at: 4787 4925 4787 Size: 138 8134 SalI G'TCGA_C Cuts at: 1936 3242 4959 5614 1936 Size: 1306 1717 655 4594 Fragments arranged by size: 4594 1717 1306 655 SapI GCTCTTCn'nnn_ Cuts at: 2480 5209 2480 Size: 2729 5543 SbfI CC_TGCA'GG Cuts at: 5624 5624 Size: 8272 ScaI AGT'ACT Cuts at: 3224 3593 6520 8232 3224 Size: 369 2927 1712 3264 Fragments arranged by size: 3264 2927 1712 369 SfcI C'TryA_G Cuts at: 826 1045 3276 4425 4969 5620 6755 7436 7627 Size: 219 2231 1149 544 651 1135 681 191 Cuts at: 7627 826 Size: 1471 Fragments arranged by size: 2231 1471 1149 1135 681 651 544 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8272 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8272 SmaI CCC'GGG Cuts at: 1872 5599 1872 Size: 3727 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2651 2783 6376 7277 7539 820 Size: 197 926 708 132 3593 901 262 1553 Fragments arranged by size: 3593 1553 926 901 708 262 197 132 SnaBI TAC'GTA Cuts at: 493 6063 493 Size: 5570 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8272 SphI G_CATG'C Cuts at: 1132 4995 5630 1132 Size: 3863 635 3774 Fragments arranged by size: 3863 3774 635 SspI AAT'ATT Cuts at: 5 52 2231 2386 6196 5 Size: 47 2179 155 3810 2081 Fragments arranged by size: 3810 2179 2081 155 47 StuI AGG'CCT Cuts at: 1517 2626 1517 Size: 1109 7163 StyI C'CwwG_G Cuts at: 513 1065 2793 4083 5711 513 Size: 552 1728 1290 1628 3074 Fragments arranged by size: 3074 1728 1628 1290 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2386 2490 2844 4076 6299 6316 6469 Size: 1255 806 104 354 1232 2223 17 153 Cuts at: 6469 6654 7099 325 Size: 185 445 1498 Fragments arranged by size: 2223 1498 1255 1232 806 445 354 185 153 104 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2970 3222 3591 Size: 274 80 33 51 1443 993 252 369 Cuts at: 3591 3718 3826 3895 4387 4759 6518 8230 96 Size: 127 108 69 492 372 1759 1712 138 Fragments arranged by size: 1759 1712 1443 993 492 372 369 274 252 138 127 108 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2322 2354 3659 3691 4627 4659 4895 4927 8232 Size: 32 1305 32 936 32 236 32 3305 Cuts at: 8232 8264 2322 Size: 32 2330 Fragments arranged by size: 3305 2330 1305 936 236 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3774 1316 Size: 2458 5814 XbaI T'CTAG_A Cuts at: 4508 5608 4508 Size: 1100 7172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2230 4081 2230 Size: 1851 6421 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8272 XmaI C'CCGG_G Cuts at: 1870 5597 1870 Size: 3727 4545 XmnI GAAnn'nnTTC Cuts at: 3072 5203 5472 6401 3072 Size: 2131 269 929 4943 Fragments arranged by size: 4943 2131 929 269 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6080 276 Size: 53 83 186 936 4546 2468 Fragments arranged by size: 4546 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI