(Linear) (Six-base) MAPSORT of: KIEE4273.seq Check: 3071 from: 1 to: 3567 KIEE4273.orf With 182 enzymes: SgfI * July 3, 2012 18:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2311 3567 Size: 2311 1256 AccI GT'mk_AC Cuts at: 0 771 1238 1433 2955 3567 Size: 771 467 195 1522 612 Fragments arranged by size: 1522 771 612 467 195 Acc65I G'GTAC_C Cuts at: 0 1673 3567 Size: 1673 1894 AclI AA'CG_TT Cuts at: 0 1007 3567 Size: 1007 2560 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 839 1412 1552 1724 2543 2762 3567 Size: 839 573 140 172 819 219 805 Fragments arranged by size: 839 819 805 573 219 172 140 AflIII A'CryG_T Cuts at: 0 1032 2373 3567 Size: 1032 1341 1194 Fragments arranged by size: 1341 1194 1032 AhdI GACnn_n'nnGTC Cuts at: 0 3353 3567 Size: 3353 214 AleI CACnn'nnGTG Cuts at: 0 3055 3567 Size: 3055 512 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2271 3567 Size: 2271 1296 AlwNI CAG_nnn'CTG Cuts at: 0 3052 3567 Size: 3052 515 ApoI r'AATT_y Cuts at: 0 441 633 814 1082 1290 1850 2182 3228 Size: 441 192 181 268 208 560 332 1046 Cuts at: 3228 3508 3567 Size: 280 59 Fragments arranged by size: 1046 560 441 332 280 268 208 192 181 59 AseI AT'TA_AT Cuts at: 0 3178 3208 3567 Size: 3178 30 359 Fragments arranged by size: 3178 359 30 BanI G'GyrC_C Cuts at: 0 1673 2480 3567 Size: 1673 807 1087 Fragments arranged by size: 1673 1087 807 BanII G_rGCy'C Cuts at: 0 470 3567 Size: 470 3097 BbsI GAAGACnn'nnnn_ Cuts at: 0 248 1526 2800 3567 Size: 248 1278 1274 767 Fragments arranged by size: 1278 1274 767 248 BbvCI CC'TCA_GC Cuts at: 0 535 3567 Size: 535 3032 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2787 2821 3567 Size: 2787 34 746 Fragments arranged by size: 2787 746 34 BclI T'GATC_A Cuts at: 0 975 1593 1665 3567 Size: 975 618 72 1902 Fragments arranged by size: 1902 975 618 72 BglI GCCn_nnn'nGGC Cuts at: 0 736 3567 Size: 736 2831 BlpI GC'TnA_GC Cuts at: 0 2783 3567 Size: 2783 784 BmgBI CAC'GTC Cuts at: 0 2058 3567 Size: 2058 1509 BmrI ACTGGGnnnn_n' Cuts at: 0 1340 2045 3567 Size: 1340 705 1522 Fragments arranged by size: 1522 1340 705 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 560 2632 3567 Size: 560 2072 935 Fragments arranged by size: 2072 935 560 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1844 2198 3567 Size: 1844 354 1369 Fragments arranged by size: 1844 1369 354 Bpu10I CC'TnA_GC Cuts at: 0 535 1579 3567 Size: 535 1044 1988 Fragments arranged by size: 1988 1044 535 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 667 763 3567 Size: 667 96 2804 Fragments arranged by size: 2804 667 96 BsaAI yAC'GTr Cuts at: 0 840 3567 Size: 840 2727 BsaBI GATnn'nnATC Cuts at: 0 1619 2672 3567 Size: 1619 1053 895 Fragments arranged by size: 1619 1053 895 BsaHI Gr'CG_yC Cuts at: 0 1972 3567 Size: 1972 1595 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 272 302 553 583 3567 Size: 272 30 251 30 2984 Fragments arranged by size: 2984 272 251 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 479 3567 Size: 479 3088 BseYI C'CCAG_C Cuts at: 0 1155 2557 3567 Size: 1155 1402 1010 Fragments arranged by size: 1402 1155 1010 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 584 636 862 1036 3047 3414 3567 Size: 584 52 226 174 2011 367 153 Fragments arranged by size: 2011 584 367 226 174 153 52 BsiHKAI G_wGCw'C Cuts at: 0 544 1650 3068 3567 Size: 544 1106 1418 499 Fragments arranged by size: 1418 1106 544 499 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1963 2975 3567 Size: 1963 1012 592 Fragments arranged by size: 1963 1012 592 Bsp1286I G_dGCh'C Cuts at: 0 470 544 1650 3068 3567 Size: 470 74 1106 1418 499 Fragments arranged by size: 1418 1106 499 470 74 BspHI T'CATG_A Cuts at: 0 1877 3567 Size: 1877 1690 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2311 3567 Size: 2311 1256 BsrDI GCAATG_nn' Cuts at: 0 2794 2946 3567 Size: 2794 152 621 Fragments arranged by size: 2794 621 152 BsrGI T'GTAC_A Cuts at: 0 1890 2754 3567 Size: 1890 864 813 Fragments arranged by size: 1890 864 813 BstAPI GCAn_nnn'nTGC Cuts at: 0 614 2014 2992 3567 Size: 614 1400 978 575 Fragments arranged by size: 1400 978 614 575 BstEII G'GTnAC_C Cuts at: 0 807 1260 3567 Size: 807 453 2307 Fragments arranged by size: 2307 807 453 BstXI CCAn_nnnn'nTGG Cuts at: 0 517 2111 3567 Size: 517 1594 1456 Fragments arranged by size: 1594 1456 517 Bsu36I CC'TnA_GG Cuts at: 0 480 3567 Size: 480 3087 BtgI C'CryG_G Cuts at: 0 788 3567 Size: 788 2779 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 496 3567 Size: 496 3071 BtsI GCAGTG_nn' Cuts at: 0 1182 2253 3567 Size: 1182 1071 1314 Fragments arranged by size: 1314 1182 1071 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 659 694 3567 Size: 659 35 2873 Fragments arranged by size: 2873 659 35 DraI TTT'AAA Cuts at: 0 2216 2456 2684 3395 3567 Size: 2216 240 228 711 172 Fragments arranged by size: 2216 711 240 228 172 DraIII CAC_nnn'GTG Cuts at: 0 346 3567 Size: 346 3221 EaeI y'GGCC_r Cuts at: 0 1548 3567 Size: 1548 2019 EarI CTCTTCn'nnn_ Cuts at: 0 475 497 582 1244 2781 3204 3481 3567 Size: 475 22 85 662 1537 423 277 86 Fragments arranged by size: 1537 662 475 423 277 86 85 22 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 839 1412 1552 1724 1844 2198 2543 2762 Size: 839 573 140 172 120 354 345 219 Cuts at: 2762 3567 Size: 805 Fragments arranged by size: 839 805 573 354 345 219 172 140 120 EcoO109I rG'GnC_Cy Cuts at: 0 484 3567 Size: 484 3083 EcoRI G'AATT_C Cuts at: 0 441 814 3567 Size: 441 373 2753 Fragments arranged by size: 2753 441 373 EcoRV GAT'ATC Cuts at: 0 2441 3567 Size: 2441 1126 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2376 3567 Size: 2376 1191 HaeII r_GCGC'y Cuts at: 0 3142 3567 Size: 3142 425 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 640 672 687 719 1755 1787 2157 2189 Size: 640 32 15 32 1036 32 370 32 Cuts at: 2189 2485 2517 3339 3371 3567 Size: 296 32 822 32 196 Fragments arranged by size: 1036 822 640 370 296 196 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 0 1239 1463 1534 2136 2915 2956 3567 Size: 1239 224 71 602 779 41 611 Fragments arranged by size: 1239 779 611 602 224 71 41 HindIII A'AGCT_T Cuts at: 0 100 1555 3567 Size: 100 1455 2012 Fragments arranged by size: 2012 1455 100 HpaI GTT'AAC Cuts at: 0 1463 3567 Size: 1463 2104 KpnI G_GTAC'C Cuts at: 0 1677 3567 Size: 1677 1890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1380 1701 1772 1910 3567 Size: 1380 321 71 138 1657 Fragments arranged by size: 1657 1380 321 138 71 MslI CAynn'nnrTG Cuts at: 0 515 1982 2109 3055 3567 Size: 515 1467 127 946 512 Fragments arranged by size: 1467 946 515 512 127 MspA1I CmG'CkG Cuts at: 0 2782 2920 3567 Size: 2782 138 647 Fragments arranged by size: 2782 647 138 NcoI C'CATG_G Cuts at: 0 788 3567 Size: 788 2779 NdeI CA'TA_TG Cuts at: 0 906 3567 Size: 906 2661 NspI r_CATG'y Cuts at: 0 581 1036 2377 2913 2990 3567 Size: 581 455 1341 536 77 577 Fragments arranged by size: 1341 581 577 536 455 77 PasI CC'CwG_GG Cuts at: 0 826 3567 Size: 826 2741 PciI A'CATG_T Cuts at: 0 1032 2373 3567 Size: 1032 1341 1194 Fragments arranged by size: 1341 1194 1032 PflMI CCAn_nnn'nTGG Cuts at: 0 1630 2977 3567 Size: 1630 1347 590 Fragments arranged by size: 1630 1347 590 PfoI T'CCnGG_A Cuts at: 0 129 2175 3567 Size: 129 2046 1392 Fragments arranged by size: 2046 1392 129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 868 900 3567 Size: 868 32 2667 Fragments arranged by size: 2667 868 32 PpuMI rG'GwC_Cy Cuts at: 0 484 3567 Size: 484 3083 PstI C_TGCA'G Cuts at: 0 2424 3567 Size: 2424 1143 PvuII CAG'CTG Cuts at: 0 2782 2920 3567 Size: 2782 138 647 Fragments arranged by size: 2782 647 138 SalI G'TCGA_C Cuts at: 0 1237 2954 3567 Size: 1237 1717 613 Fragments arranged by size: 1717 1237 613 SapI GCTCTTCn'nnn_ Cuts at: 0 475 3204 3567 Size: 475 2729 363 Fragments arranged by size: 2729 475 363 ScaI AGT'ACT Cuts at: 0 1219 1588 3567 Size: 1219 369 1979 Fragments arranged by size: 1979 1219 369 SfcI C'TryA_G Cuts at: 0 1271 2420 2964 3567 Size: 1271 1149 544 603 Fragments arranged by size: 1271 1149 603 544 SmlI C'TyrA_G Cuts at: 0 646 778 3567 Size: 646 132 2789 Fragments arranged by size: 2789 646 132 SphI G_CATG'C Cuts at: 0 2990 3567 Size: 2990 577 SspI AAT'ATT Cuts at: 0 226 381 3567 Size: 226 155 3186 Fragments arranged by size: 3186 226 155 StuI AGG'CCT Cuts at: 0 621 3567 Size: 621 2946 StyI C'CwwG_G Cuts at: 0 788 2078 3567 Size: 788 1290 1489 Fragments arranged by size: 1489 1290 788 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 381 485 839 2071 3567 Size: 381 104 354 1232 1496 Fragments arranged by size: 1496 1232 381 354 104 TatI w'GTAC_w Cuts at: 0 965 1217 1586 1713 1821 1890 2382 2754 Size: 965 252 369 127 108 69 492 372 Cuts at: 2754 3567 Size: 813 Fragments arranged by size: 965 813 492 372 369 252 127 108 69 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 317 349 1654 1686 2622 2654 2890 2922 Size: 317 32 1305 32 936 32 236 32 Cuts at: 2922 3567 Size: 645 Fragments arranged by size: 1305 936 645 317 236 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1769 3567 Size: 1769 1798 XbaI T'CTAG_A Cuts at: 0 2503 3567 Size: 2503 1064 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 225 2076 3567 Size: 225 1851 1491 Fragments arranged by size: 1851 1491 225 XmnI GAAnn'nnTTC Cuts at: 0 1067 3198 3467 3567 Size: 1067 2131 269 100 Fragments arranged by size: 2131 1067 269 100 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI AlwNI ApoI AseI BanI BanII BbsI BbvCI BcgI BclI BglI BlpI BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstEII BstXI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI KpnI MmeI MslI MspA1I NcoI NdeI NspI PasI PciI PflMI PfoI PpiI PpuMI PstI PvuII SalI SapI ScaI SfcI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AatII AfeI AflII AgeI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BciVI BfrBI BglII Bme1580I BmtI BsaI BsaWI BsiEI BsiWI BsmI BspEI BsrBI BsrFI BssHII BssSI BstBI BstYI BstZ17I ClaI DrdI EagI EciI EcoICRI EcoNI FseI FspI FspAI KasI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI XhoI XmaI ZraI