################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KspI MaeII MamI MfeI MluI MroI MslI MspI MunI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 127 GGTACC G^GTACC AccI 555 GTCTAC GT^[AC][GT]AC AccII 425 CGCG CG^CG AcsI 912 GAATTT [GA]^AATT[CT] AluI 72 AGCT AG^CT AlwI 3 GGATC GGATC(4/5) AlwNI 464 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 912 GAATTT [GA]^AATT[CT] Asp718I 127 GGTACC G^GTACC AvaII 404 GGTCC G^G[AT]CC BalI 251 TGGCCA TGG^CCA BanI 127 GGTACC G^G[CT][GA]CC BbvCI 474 CCTCAGC CCTCAGC(-5/-2) BfaI 70 CTAG C^TAG BseRI 702 GAGGAG GAGGAG(10/8) BsmFI 405 GTCCC GGGAC(10/14) Bsp1407I 107 TGTACA T^GTACA BspHI 510 TCATGA T^CATGA BsrGI 107 TGTACA T^GTACA BstUI 425 CGCG CG^CG BstYI 3 GGATCT [GA]^GATC[CT] CfoI 426 GCGC GCG^C Cfr13I 404 GGTCC G^G[ACGT]CC DraI 712 TTTAAA TTT^AAA DraIII 394 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 389 CTCTTC CTCTTC(1/4) FauI 423 CCCGC CCCGC(4/6) HhaI 426 GCGC GCG^C HinP1I 426 GCGC G^CGC KpnI 127 GGTACC GGTAC^C Ksp632I 389 CTCTTC CTCTTC(1/4) MaeI 70 CTAG C^TAG MflI 3 GGATCT [GA]^GATC[CT] MluNI 251 TGGCCA TGG^CCA MscI 251 TGGCCA TGG^CCA MseI 713 TTAA T^TAA MspA1I 467 CCGCTG C[AC]G^C[GT]G MvnI 425 CGCG CG^CG RcaI 510 TCATGA T^CATGA Sau96I 404 GGTCC G^G[ACGT]CC SfcI 980 CTGTAG C^T[GA][CT]AG SspBI 107 TGTACA T^GTACA Tru9I 713 TTAA T^TAA XcmI 437 CCAGCATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 3 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 108 GTAC GT^AC 128 GTAC BbsI 307 GTCTTC GAAGAC(2/6) 925 GTCTTC BbvI 411 GCAGC GCAGC(8/12) 469 GCTGC BclI 8 TGATCA T^GATCA 29 TGATCA BpuAI 307 GTCTTC GAAGAC(2/6) 925 GTCTTC BsaJI 447 CCTGGG C^C[ACGT][ACGT]GG 792 CCAGGG BsiYI 422 CCCCGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 423 CCCGCGCTGGG BslI 422 CCCCGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 423 CCCGCGCTGGG BsmAI 502 GAGAC GTCTC(1/5) 324 GTCTC BssKI 447 CCTGG ^CC[ACGT]GG 792 CCAGG BstNI 447 CCTGG CC^[AT]GG 792 CCAGG EaeI 251 TGGCCA [CT]^GGCC[GA] 359 CGGCCA EcoRII 447 CCTGG ^CC[AT]GG 792 CCAGG FbaI 8 TGATCA T^GATCA 29 TGATCA HincII 834 GTTGAC GT[CT]^[GA]AC 957 GTCAAC HindII 834 GTTGAC GT[CT]^[GA]AC 957 GTCAAC MvaI 447 CCTGG CC^[AT]GG 792 CCAGG NlaIII 511 CATG CATG^ 604 CATG PleI 452 GAGTC GAGTC(4/5) 955 GAGTC RsaI 108 GTAC GT^AC 128 GTAC ScrFI 447 CCTGG CC^[ACGT]GG 792 CCAGG TseI 411 GCAGC G^C[AT]GC 469 GCTGC Tsp45I 529 GTGAC ^GT[GC]AC 819 GTCAC Tsp509I 123 AATT ^AATT 913 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AciI BsrI BstF5I Cac8I DdeI DpnII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI NdeII NlaIV Sau3AI SfaNI TfiI TspRI