(Circular) (Six-base) MAPSORT of: pFN21AE3807.seq Check: 7750 from: 1 to: 5704 pFN21AE3807 5704 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3514 278 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2561 3047 1937 Size: 624 486 4594 Fragments arranged by size: 4594 624 486 Acc65I G'GTAC_C Cuts at: 2132 3025 2132 Size: 893 4811 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3767 5151 5419 1632 Size: 2135 1384 268 1917 Fragments arranged by size: 2135 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 4603 5580 1203 Size: 3400 977 1327 Fragments arranged by size: 3400 1327 977 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 4433 4433 Size: 5704 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5704 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2600 1184 Size: 1416 4288 AlwNI CAG_nnn'CTG Cuts at: 2474 3447 5019 2474 Size: 973 1572 3159 Fragments arranged by size: 3159 1572 973 ApaLI G'TGCA_C Cuts at: 3761 4917 3761 Size: 1156 4548 ApoI r'AATT_y Cuts at: 1493 2917 3013 3239 3391 5500 1493 Size: 1424 96 226 152 2109 1697 Fragments arranged by size: 2109 1697 1424 226 152 96 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 3029 1870 Size: 73 1086 4545 Fragments arranged by size: 4545 1086 73 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 3025 4480 618 Size: 325 217 627 345 893 1455 1842 Fragments arranged by size: 1842 1455 893 627 345 325 217 BanII G_rGCy'C Cuts at: 1391 3023 1391 Size: 1632 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2305 2923 928 Size: 971 406 618 3709 Fragments arranged by size: 3709 971 618 406 BbvCI CC'TCA_GC Cuts at: 2480 2480 Size: 5704 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3895 3929 1903 Size: 34 1958 34 3678 Fragments arranged by size: 3678 1958 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 2013 2034 1548 Size: 465 21 5218 Fragments arranged by size: 5218 465 21 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4315 136 Size: 107 122 71 631 605 2643 1525 Fragments arranged by size: 2643 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5699 5699 Size: 5704 BlpI GC'TnA_GC Cuts at: 3121 3121 Size: 5704 Bme1580I G_kGCm'C Cuts at: 1790 3765 4921 1790 Size: 1975 1156 2573 Fragments arranged by size: 2573 1975 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2396 2578 3435 Size: 416 53 83 399 24 979 182 857 Cuts at: 3435 4393 442 Size: 958 1753 Fragments arranged by size: 1753 979 958 857 416 399 182 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4364 1239 Size: 3125 2579 Bpu10I CC'TnA_GC Cuts at: 2480 2480 Size: 5704 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3829 4694 4992 3829 Size: 865 298 4541 Fragments arranged by size: 4541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4367 882 Size: 617 2868 2219 Fragments arranged by size: 2868 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3495 493 Size: 711 2291 2702 Fragments arranged by size: 2702 2291 711 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3511 3893 4528 275 Size: 53 83 186 936 1978 382 635 1451 Fragments arranged by size: 1978 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4137 4535 4809 4956 5367 4137 Size: 398 274 147 411 4474 Fragments arranged by size: 4474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5479 Fragments arranged by size: 5479 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2722 1186 Size: 1536 4168 BseYI C'CCAG_C Cuts at: 2412 2433 2638 4907 5403 2412 Size: 21 205 2269 496 2713 Fragments arranged by size: 2713 2269 496 205 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5131 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3154 3915 4943 1540 Size: 275 186 1153 761 1028 2301 Fragments arranged by size: 2301 1153 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3765 3850 4921 3765 Size: 85 1071 4548 Fragments arranged by size: 4548 1071 85 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3023 3765 3850 4921 1391 Size: 399 1233 742 85 1071 2174 Fragments arranged by size: 2174 1233 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 2515 3593 5323 2515 Size: 1078 1730 2896 Fragments arranged by size: 2896 1730 1078 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3059 844 Size: 2215 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3591 1394 Size: 429 1768 3507 Fragments arranged by size: 3507 1768 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4348 4535 1700 Size: 110 2538 187 2869 Fragments arranged by size: 2869 2538 187 110 BsrGI T'GTAC_A Cuts at: 96 2112 96 Size: 2016 3688 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5704 BssSI C'ACGA_G Cuts at: 3764 4776 3764 Size: 1012 4692 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5704 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstYI r'GATC_y Cuts at: 1972 2008 3034 3401 3786 3803 5255 5341 5353 Size: 36 1026 367 385 17 1452 86 12 Cuts at: 5353 5699 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1026 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4213 Fragments arranged by size: 4213 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 2606 3220 4014 4034 2606 Size: 614 794 20 4276 Fragments arranged by size: 4276 794 614 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 2719 3008 2719 Size: 289 5415 DraIII CAC_nnn'GTG Cuts at: 2404 2404 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4711 809 Size: 507 3395 1802 Fragments arranged by size: 3395 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 2364 3151 Size: 54 1425 50 275 18 426 108 787 Cuts at: 3151 4040 5669 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 787 426 275 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3151 1537 Size: 275 1339 4090 Fragments arranged by size: 4090 1339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2400 3634 1353 Size: 1047 1234 3423 Fragments arranged by size: 3423 1234 1047 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4291 4665 4811 387 Size: 1064 138 2702 374 146 1280 Fragments arranged by size: 2702 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3767 4364 5151 5419 1239 Size: 393 2135 597 787 268 1524 Fragments arranged by size: 2135 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FspI TGC'GCA Cuts at: 4210 4210 Size: 5704 HaeII r_GCGC'y Cuts at: 1678 4851 1678 Size: 3173 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4345 4377 4419 4451 1373 Size: 32 135 32 2773 32 42 32 2626 Fragments arranged by size: 2773 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2841 2964 3048 3294 669 Size: 988 281 903 123 84 246 3079 Fragments arranged by size: 3079 988 903 281 246 123 84 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KpnI G_GTAC'C Cuts at: 2136 3029 2136 Size: 893 4811 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2756 4818 5002 1305 Size: 1451 2062 184 2007 Fragments arranged by size: 2062 2007 1451 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2258 10 Size: 54 1425 343 426 3456 Fragments arranged by size: 3456 1425 426 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3662 4021 4180 518 Size: 648 2496 359 159 2042 Fragments arranged by size: 2496 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2474 3120 3797 4945 5190 1606 Size: 868 646 677 1148 245 2120 Fragments arranged by size: 2120 1148 868 677 646 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4213 Fragments arranged by size: 4213 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 3062 4607 1132 Size: 97 1833 1545 2229 Fragments arranged by size: 2229 1833 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5704 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PstI C_TGCA'G Cuts at: 830 3056 830 Size: 2226 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 3952 5664 3952 Size: 1712 3992 SfcI C'TryA_G Cuts at: 826 1045 2985 3052 4187 4868 5059 826 Size: 219 1940 67 1135 681 191 1471 Fragments arranged by size: 1940 1471 1135 681 219 191 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 3031 1872 Size: 1159 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3808 4709 4971 820 Size: 197 926 1865 901 262 1553 Fragments arranged by size: 1865 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5704 StyI C'CwwG_G Cuts at: 513 1065 2004 3143 513 Size: 552 939 1139 3074 Fragments arranged by size: 3074 1139 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2149 2360 2415 3731 3748 3901 4086 Size: 1255 569 211 55 1316 17 153 185 Cuts at: 4086 4531 325 Size: 445 1498 Fragments arranged by size: 1498 1316 1255 569 445 211 185 153 55 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 3950 5662 Size: 274 80 33 51 1443 135 1838 1712 Cuts at: 5662 96 Size: 138 Fragments arranged by size: 1838 1712 1443 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5664 5696 5664 Size: 32 5672 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2449 2449 Size: 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 3029 1870 Size: 1159 4545 XmnI GAAnn'nnTTC Cuts at: 3833 3833 Size: 5704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3512 276 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI