(Circular) (Six-base) MAPSORT of: pFN21AE3265.seq Check: 2336 from: 1 to: 6637 pFN21AE3265 6637 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4447 278 Size: 53 83 186 936 2911 2468 Fragments arranged by size: 2911 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3980 1937 Size: 2043 4594 Acc65I G'GTAC_C Cuts at: 3958 3958 Size: 6637 AclI AA'CG_TT Cuts at: 1563 3535 4764 5137 1563 Size: 1972 1229 373 3063 Fragments arranged by size: 3063 1972 1229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2961 4700 6084 6352 1632 Size: 1329 1739 1384 268 1917 Fragments arranged by size: 1917 1739 1384 1329 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6440 AflIII A'CryG_T Cuts at: 1203 2180 2536 5536 6513 1203 Size: 977 356 3000 977 1327 Fragments arranged by size: 3000 1327 977 977 356 AgeI A'CCGG_T Cuts at: 5468 5468 Size: 6637 AhdI GACnn_n'nnGTC Cuts at: 5366 5366 Size: 6637 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6637 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6637 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2051 2083 2051 Size: 32 6605 AlwNI CAG_nnn'CTG Cuts at: 2954 4380 5952 2954 Size: 1426 1572 3639 Fragments arranged by size: 3639 1572 1426 ApaLI G'TGCA_C Cuts at: 4694 5850 4694 Size: 1156 5481 ApoI r'AATT_y Cuts at: 1493 2302 3260 3946 4172 4324 6433 1493 Size: 809 958 686 226 152 2109 1697 Fragments arranged by size: 2109 1697 958 809 686 226 152 AseI AT'TA_AT Cuts at: 160 1032 5191 160 Size: 872 4159 1606 Fragments arranged by size: 4159 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 AvaI C'yCGr_G Cuts at: 1870 1943 3057 3962 1870 Size: 73 1114 905 4545 Fragments arranged by size: 4545 1114 905 73 BamHI G'GATC_C Cuts at: 2849 3967 4334 2849 Size: 1118 367 5152 Fragments arranged by size: 5152 1118 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3580 3958 5413 618 Size: 325 217 627 1793 378 1455 1842 Fragments arranged by size: 1842 1793 1455 627 378 325 217 BanII G_rGCy'C Cuts at: 1391 2516 3956 1391 Size: 1125 1440 4072 Fragments arranged by size: 4072 1440 1125 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5666 BbvCI CC'TCA_GC Cuts at: 3789 3789 Size: 6637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4828 4862 1903 Size: 34 2891 34 3678 Fragments arranged by size: 3678 2891 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4529 5739 4529 Size: 1210 5427 BclI T'GATC_A Cuts at: 1548 1548 Size: 6637 BfrBI ATG'CAT Cuts at: 3479 3479 Size: 6637 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5248 136 Size: 107 122 71 631 605 3576 1525 Fragments arranged by size: 3576 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6632 6632 Size: 6637 BlpI GC'TnA_GC Cuts at: 4054 4054 Size: 6637 Bme1580I G_kGCm'C Cuts at: 1790 3585 4698 5854 1790 Size: 1795 1113 1156 2573 Fragments arranged by size: 2573 1795 1156 1113 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6637 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2991 4368 5326 Size: 416 53 83 399 24 1574 1377 958 Cuts at: 5326 442 Size: 1753 Fragments arranged by size: 1753 1574 1377 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6637 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 5297 1239 Size: 822 3236 2579 Fragments arranged by size: 3236 2579 822 Bpu10I CC'TnA_GC Cuts at: 2254 3789 2254 Size: 1535 5102 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2365 4762 5627 5925 2365 Size: 2397 865 298 3077 Fragments arranged by size: 3077 2397 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5300 882 Size: 617 3801 2219 Fragments arranged by size: 3801 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4428 493 Size: 711 3224 2702 Fragments arranged by size: 3224 2702 711 BsaBI GATnn'nnATC Cuts at: 6631 6631 Size: 6637 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4444 4826 5461 275 Size: 53 83 186 936 2911 382 635 1451 Fragments arranged by size: 2911 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3894 5070 5468 5742 5889 6300 3894 Size: 1176 398 274 147 411 4231 Fragments arranged by size: 4231 1176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2081 2977 3007 1178 Size: 30 165 30 648 30 896 30 4808 Fragments arranged by size: 4808 896 648 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6637 BseYI C'CCAG_C Cuts at: 5840 6336 5840 Size: 496 6141 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2217 1163 Size: 573 481 5583 Fragments arranged by size: 5583 573 481 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4087 4848 5876 1540 Size: 275 186 2086 761 1028 2301 Fragments arranged by size: 2301 2086 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3064 4698 4783 5854 3064 Size: 1634 85 1071 3847 Fragments arranged by size: 3847 1634 1071 85 BsmI GAATG_Cn' Cuts at: 4148 4241 4148 Size: 93 6544 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2516 3064 3585 3956 4698 4783 5854 Size: 399 726 548 521 371 742 85 1071 Cuts at: 5854 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 726 548 521 399 371 85 BspEI T'CCGG_A Cuts at: 3894 6300 3894 Size: 2406 4231 BspHI T'CATG_A Cuts at: 4526 6256 4526 Size: 1730 4907 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3992 844 Size: 3148 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3136 4524 1394 Size: 429 1313 1388 3507 Fragments arranged by size: 3507 1388 1313 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5132 5306 65 Size: 1183 3884 174 1396 Fragments arranged by size: 3884 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5281 5468 1700 Size: 110 3471 187 2869 Fragments arranged by size: 3471 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6637 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6637 BssSI C'ACGA_G Cuts at: 3063 4697 5709 3063 Size: 1634 1012 3991 Fragments arranged by size: 3991 1634 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3883 794 Size: 3089 3548 BstBI TT'CG_AA Cuts at: 5441 5441 Size: 6637 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6637 BstXI CCAn_nnnn'nTGG Cuts at: 3592 3592 Size: 6637 BstYI r'GATC_y Cuts at: 1972 2765 2849 3915 3967 4334 4719 4736 6188 Size: 793 84 1066 52 367 385 17 1452 Cuts at: 6188 6274 6286 6632 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1066 793 385 367 346 86 84 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3657 513 Size: 552 939 1653 3493 Fragments arranged by size: 3493 1653 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5898 Fragments arranged by size: 5898 683 56 BtsI GCAGTG_nn' Cuts at: 2422 3534 4153 4947 4967 2422 Size: 1112 619 794 20 4092 Fragments arranged by size: 4092 1112 794 619 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6637 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6602 DraI TTT'AAA Cuts at: 2710 3941 2710 Size: 1231 5406 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5644 809 Size: 507 4328 1802 Fragments arranged by size: 4328 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2275 2290 4084 Size: 54 1425 50 275 18 445 15 1794 Cuts at: 4084 4973 6602 8 Size: 889 1629 43 Fragments arranged by size: 1794 1629 1425 889 445 275 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 4084 1537 Size: 275 2272 4090 Fragments arranged by size: 4090 2272 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2547 2874 4567 1353 Size: 1194 327 1693 3423 Fragments arranged by size: 3423 1693 1194 327 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5224 5598 5744 387 Size: 1064 138 3635 374 146 1280 Fragments arranged by size: 3635 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2961 4700 5297 6084 6352 1239 Size: 393 429 900 1739 597 787 268 1524 Fragments arranged by size: 1739 1524 900 787 597 429 393 268 EcoRI G'AATT_C Cuts at: 3946 4324 3946 Size: 378 6259 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2217 2343 2496 2217 Size: 126 153 6358 Fragments arranged by size: 6358 153 126 FspI TGC'GCA Cuts at: 5143 5143 Size: 6637 HaeII r_GCGC'y Cuts at: 1678 5784 1678 Size: 4106 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2741 2773 2977 3009 5278 Size: 32 135 32 1169 32 204 32 2269 Cuts at: 5278 5310 5352 5384 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2269 1169 204 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3568 3981 4227 669 Size: 988 281 1630 413 246 3079 Fragments arranged by size: 3079 1630 988 413 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6637 HpaI GTT'AAC Cuts at: 4227 4227 Size: 6637 KpnI G_GTAC'C Cuts at: 3962 3962 Size: 6637 MfeI C'AATT_G Cuts at: 4236 4236 Size: 6637 MluI A'CGCG_T Cuts at: 6513 6513 Size: 6637 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3014 3518 5751 5935 1305 Size: 1709 504 2233 184 2007 Fragments arranged by size: 2233 2007 1709 504 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2277 2292 10 Size: 54 1425 343 445 15 4355 Fragments arranged by size: 4355 1425 445 343 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2427 3174 3431 4595 4954 5113 518 Size: 648 1261 747 257 1164 359 159 2042 Fragments arranged by size: 2042 1261 1164 747 648 359 257 159 MspA1I CmG'CkG Cuts at: 1606 2593 2797 4053 4730 5878 6123 1606 Size: 987 204 1256 677 1148 245 2120 Fragments arranged by size: 2120 1256 1148 987 677 245 204 NcoI C'CATG_G Cuts at: 513 1065 2004 3657 513 Size: 552 939 1653 3493 Fragments arranged by size: 3493 1653 939 552 NdeI CA'TA_TG Cuts at: 387 2440 387 Size: 2053 4584 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6637 NotI GC'GGCC_GC Cuts at: 4084 4084 Size: 6637 NsiI A_TGCA'T Cuts at: 3481 3481 Size: 6637 NspI r_CATG'y Cuts at: 1132 1229 2184 2540 3479 3886 3995 5540 1132 Size: 97 955 356 939 407 109 1545 2229 Fragments arranged by size: 2229 1545 955 939 407 356 109 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6637 PciI A'CATG_T Cuts at: 2180 2536 5536 2180 Size: 356 3000 3281 Fragments arranged by size: 3281 3000 356 PflMI CCAn_nnn'nTGG Cuts at: 1108 3753 1108 Size: 2645 3992 PmeI GTTT'AAAC Cuts at: 3941 3941 Size: 6637 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2051 2083 4706 4738 6219 6251 2051 Size: 32 2623 32 1481 32 2437 Fragments arranged by size: 2623 2437 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6637 PsiI TTA'TAA Cuts at: 2205 4207 2205 Size: 2002 4635 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3132 3164 3132 Size: 32 6605 PstI C_TGCA'G Cuts at: 830 3686 3989 830 Size: 2856 303 3478 Fragments arranged by size: 3478 2856 303 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6637 PvuII CAG'CTG Cuts at: 2593 2797 2593 Size: 204 6433 SalI G'TCGA_C Cuts at: 1936 3979 1936 Size: 2043 4594 SbfI CC_TGCA'GG Cuts at: 3989 3989 Size: 6637 ScaI AGT'ACT Cuts at: 4885 6597 4885 Size: 1712 4925 SfcI C'TryA_G Cuts at: 826 1045 2597 2625 3682 3985 5120 5801 5992 Size: 219 1552 28 1057 303 1135 681 191 Cuts at: 5992 826 Size: 1471 Fragments arranged by size: 1552 1471 1135 1057 681 303 219 191 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6637 SmaI CCC'GGG Cuts at: 1872 3964 1872 Size: 2092 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2380 3057 4741 5642 5904 820 Size: 197 926 437 677 1684 901 262 1553 Fragments arranged by size: 1684 1553 926 901 677 437 262 197 SnaBI TAC'GTA Cuts at: 493 4428 493 Size: 3935 2702 SpeI A'CTAG_T Cuts at: 152 3041 152 Size: 2889 3748 SphI G_CATG'C Cuts at: 1132 3479 3995 1132 Size: 2347 516 3774 Fragments arranged by size: 3774 2347 516 SspI AAT'ATT Cuts at: 5 52 3640 4561 5 Size: 47 3588 921 2081 Fragments arranged by size: 3588 2081 921 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6637 StyI C'CwwG_G Cuts at: 513 1065 2004 3657 4076 513 Size: 552 939 1653 419 3074 Fragments arranged by size: 3074 1653 939 552 419 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2184 3650 4664 4681 4834 5019 5464 Size: 1255 604 1466 1014 17 153 185 445 Cuts at: 5464 325 Size: 1498 Fragments arranged by size: 1498 1466 1255 1014 604 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2543 3148 3598 Size: 274 80 33 51 1443 566 605 450 Cuts at: 3598 4883 6595 96 Size: 1285 1712 138 Fragments arranged by size: 1712 1443 1285 605 566 450 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2632 2664 6597 6629 2632 Size: 32 3933 32 2640 Fragments arranged by size: 3933 2640 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6637 XbaI T'CTAG_A Cuts at: 3973 3973 Size: 6637 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2285 2478 2285 Size: 193 6444 XhoI C'TCGA_G Cuts at: 1943 3057 1943 Size: 1114 5523 XmaI C'CCGG_G Cuts at: 1870 3962 1870 Size: 2092 4545 XmnI GAAnn'nnTTC Cuts at: 3345 4766 3345 Size: 1421 5216 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4445 276 Size: 53 83 186 936 2911 2468 Fragments arranged by size: 2911 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI