(Linear) (Six-base) MAPSORT of: KIEE3265.seq Check: 4074 from: 1 to: 1932 KIEE3265.orf With 182 enzymes: SgfI * July 3, 2012 17:32 .. AclI AA'CG_TT Cuts at: 0 1530 1932 Size: 1530 402 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 956 1932 Size: 956 976 AflIII A'CryG_T Cuts at: 0 175 531 1932 Size: 175 356 1401 Fragments arranged by size: 1401 356 175 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 46 78 1932 Size: 46 32 1854 Fragments arranged by size: 1854 46 32 AlwNI CAG_nnn'CTG Cuts at: 0 949 1932 Size: 949 983 ApoI r'AATT_y Cuts at: 0 297 1255 1932 Size: 297 958 677 Fragments arranged by size: 958 677 297 AvaI C'yCGr_G Cuts at: 0 1052 1932 Size: 1052 880 BamHI G'GATC_C Cuts at: 0 844 1932 Size: 844 1088 BanI G'GyrC_C Cuts at: 0 1575 1932 Size: 1575 357 BanII G_rGCy'C Cuts at: 0 511 1932 Size: 511 1421 BbvCI CC'TCA_GC Cuts at: 0 1784 1932 Size: 1784 148 BfrBI ATG'CAT Cuts at: 0 1474 1932 Size: 1474 458 Bme1580I G_kGCm'C Cuts at: 0 1580 1932 Size: 1580 352 BmrI ACTGGGnnnn_n' Cuts at: 0 986 1932 Size: 986 946 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 1932 Size: 56 1876 Bpu10I CC'TnA_GC Cuts at: 0 249 1784 1932 Size: 249 1535 148 Fragments arranged by size: 1535 249 148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 360 1932 Size: 360 1572 BsaWI w'CCGG_w Cuts at: 0 1889 1932 Size: 1889 43 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 46 76 972 1002 1932 Size: 46 30 896 30 930 Fragments arranged by size: 930 896 46 30 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 212 1932 Size: 212 1720 BsiHKAI G_wGCw'C Cuts at: 0 1059 1932 Size: 1059 873 Bsp1286I G_dGCh'C Cuts at: 0 511 1059 1580 1932 Size: 511 548 521 352 Fragments arranged by size: 548 521 511 352 BspEI T'CCGG_A Cuts at: 0 1889 1932 Size: 1889 43 BsrBI CCG'CTC Cuts at: 0 1131 1932 Size: 1131 801 BssSI C'ACGA_G Cuts at: 0 1058 1932 Size: 1058 874 BstAPI GCAn_nnn'nTGC Cuts at: 0 1878 1932 Size: 1878 54 BstXI CCAn_nnnn'nTGG Cuts at: 0 1587 1932 Size: 1587 345 BstYI r'GATC_y Cuts at: 0 760 844 1910 1932 Size: 760 84 1066 22 Fragments arranged by size: 1066 760 84 22 BtgI C'CryG_G Cuts at: 0 1652 1932 Size: 1652 280 BtsI GCAGTG_nn' Cuts at: 0 417 1529 1932 Size: 417 1112 403 Fragments arranged by size: 1112 417 403 DraI TTT'AAA Cuts at: 0 705 1932 Size: 705 1227 EaeI y'GGCC_r Cuts at: 0 270 285 1932 Size: 270 15 1647 Fragments arranged by size: 1647 270 15 EarI CTCTTCn'nnn_ Cuts at: 0 542 869 1932 Size: 542 327 1063 Fragments arranged by size: 1063 542 327 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 956 1932 Size: 56 900 976 Fragments arranged by size: 976 900 56 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 212 338 491 1932 Size: 212 126 153 1441 Fragments arranged by size: 1441 212 153 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 736 768 972 1004 1932 Size: 736 32 204 32 928 Fragments arranged by size: 928 736 204 32 32 HincII GTy'rAC Cuts at: 0 1563 1932 Size: 1563 369 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1009 1513 1932 Size: 1009 504 419 Fragments arranged by size: 1009 504 419 MscI TGG'CCA Cuts at: 0 272 287 1932 Size: 272 15 1645 Fragments arranged by size: 1645 272 15 MslI CAynn'nnrTG Cuts at: 0 422 1169 1426 1932 Size: 422 747 257 506 Fragments arranged by size: 747 506 422 257 MspA1I CmG'CkG Cuts at: 0 588 792 1932 Size: 588 204 1140 Fragments arranged by size: 1140 588 204 NcoI C'CATG_G Cuts at: 0 1652 1932 Size: 1652 280 NdeI CA'TA_TG Cuts at: 0 435 1932 Size: 435 1497 NsiI A_TGCA'T Cuts at: 0 1476 1932 Size: 1476 456 NspI r_CATG'y Cuts at: 0 179 535 1474 1881 1932 Size: 179 356 939 407 51 Fragments arranged by size: 939 407 356 179 51 PciI A'CATG_T Cuts at: 0 175 531 1932 Size: 175 356 1401 Fragments arranged by size: 1401 356 175 PflMI CCAn_nnn'nTGG Cuts at: 0 1748 1932 Size: 1748 184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 46 78 1932 Size: 46 32 1854 Fragments arranged by size: 1854 46 32 PsiI TTA'TAA Cuts at: 0 200 1932 Size: 200 1732 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1127 1159 1932 Size: 1127 32 773 Fragments arranged by size: 1127 773 32 PstI C_TGCA'G Cuts at: 0 1681 1932 Size: 1681 251 PvuII CAG'CTG Cuts at: 0 588 792 1932 Size: 588 204 1140 Fragments arranged by size: 1140 588 204 SfcI C'TryA_G Cuts at: 0 592 620 1677 1932 Size: 592 28 1057 255 Fragments arranged by size: 1057 592 255 28 SmlI C'TyrA_G Cuts at: 0 375 1052 1932 Size: 375 677 880 Fragments arranged by size: 880 677 375 SpeI A'CTAG_T Cuts at: 0 1036 1932 Size: 1036 896 SphI G_CATG'C Cuts at: 0 1474 1932 Size: 1474 458 SspI AAT'ATT Cuts at: 0 1635 1932 Size: 1635 297 StyI C'CwwG_G Cuts at: 0 1652 1932 Size: 1652 280 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 179 1645 1932 Size: 179 1466 287 Fragments arranged by size: 1466 287 179 TatI w'GTAC_w Cuts at: 0 538 1143 1593 1932 Size: 538 605 450 339 Fragments arranged by size: 605 538 450 339 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 627 659 1932 Size: 627 32 1273 Fragments arranged by size: 1273 627 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 280 473 1932 Size: 280 193 1459 Fragments arranged by size: 1459 280 193 XhoI C'TCGA_G Cuts at: 0 1052 1932 Size: 1052 880 XmnI GAAnn'nnTTC Cuts at: 0 1340 1932 Size: 1340 592 Enzymes that do cut: AclI AcuI AflIII AloI AlwNI ApoI AvaI BamHI BanI BanII BbvCI BfrBI Bme1580I BmrI BpmI Bpu10I BpuEI BsaWI BsaXI BsgI BsiHKAI Bsp1286I BspEI BsrBI BssSI BstAPI BstXI BstYI BtgI BtsI DraI EaeI EarI Eco57MI FalI Hin4I HincII MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PciI PflMI PpiI PsiI PsrI PstI PvuII SfcI SmlI SpeI SphI SspI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI BciVI BclI BglI BglII BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI StuI SwaI Tth111I XbaI XmaI ZraI