################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AlwI AlwNI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BfrI BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III EcoNI EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MroI MseI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfuI SgrAI SnaBI SpeI SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 145 GAATTC [GA]^AATT[CT] AocI 286 CCTCAGG CC^T[ACGT]AGG ApaI 62 GGGCCC GGGCC^C ApoI 145 GAATTC [GA]^AATT[CT] BalI 605 TGGCCA TGG^CCA BbsI 649 GTCTTC GAAGAC(2/6) BclI 551 TGATCA T^GATCA BglII 1049 AGATCT A^GATCT BlpI 681 GCTCAGC GC^T[ACGT]AGC Bpu1102I 681 GCTCAGC GC^T[ACGT]AGC BpuAI 649 GTCTTC GAAGAC(2/6) BsmFI 242 GGGAC GGGAC(10/14) BsrDI 511 GCAATG GCAATG(2/0) BsrI 543 CCAGT ACTGG(1/-1) BssHII 437 GCGCGC G^CGCGC BssSI 328 CACGAG CACGAG(-5/-1) BstYI 1049 AGATCT [GA]^GATC[CT] Bsu36I 286 CCTCAGG CC^T[ACGT]AGG CelII 681 GCTCAGC GC^T[ACGT]AGC Eco81I 286 CCTCAGG CC^T[ACGT]AGG EcoRI 145 GAATTC G^AATTC FbaI 551 TGATCA T^GATCA MflI 1049 AGATCT [GA]^GATC[CT] MluNI 605 TGGCCA TGG^CCA MscI 605 TGGCCA TGG^CCA MslI 399 CACTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 248 CATATG CA^TATG NspI 1028 GCATGC [GA]CATG^[CT] PstI 505 CTGCAG CTGCA^G SbfI 504 CCTGCAGG CCTGCA^GG SfcI 505 CTGCAG C^T[GA][CT]AG SfiI 291 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1002 CCCGGG CCC^GGG SmlI 1237 CTCAAG C^T[CT][GA]AG SphI 1028 GCATGC GCATG^C Sse8387I 504 CCTGCAGG CCTGCA^GG Tsp509I 146 AATT ^AATT XhoII 1049 AGATCT [GA]^GATC[CT] XmaCI 1002 CCCGGG C^CCGGG XmaI 1002 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Alw44I 7 GTGCAC G^TGCAC 913 GTGCAC ApaLI 7 GTGCAC G^TGCAC 913 GTGCAC AspHI 7 GTGCAC G[AT]GC[AT]^C 913 GTGCAC BfaI 538 CTAG C^TAG 895 CTAG BseRI 284 CTCCTC GAGGAG(10/8) 331 GAGGAG BsiEI 125 CGGCCG CG[GA][CT]^CG 666 CGGCCG BsiHKAI 7 GTGCAC G[AT]GC[AT]^C 913 GTGCAC BsmBI 74 CGTCTC CGTCTC(1/5) 946 GAGACG BspMI 503 ACCTGC ACCTGC(4/8) 845 ACCTGC BsrBI 109 CCGCTC CCGCTC(-3/-3) 1086 CCGCTC DpnII 552 GATC ^GATC 1050 GATC DsaI 23 CCATGG C^C[GA][CT]GG 1170 CCATGG EagI 125 CGGCCG C^GGCCG 666 CGGCCG EclXI 125 CGGCCG C^GGCCG 666 CGGCCG Eco52I 125 CGGCCG C^GGCCG 666 CGGCCG HinfI 731 GACTC G^A[ACGT]TC 1231 GAGTC MaeI 538 CTAG C^TAG 895 CTAG MaeII 691 ACGT A^CGT 938 ACGT MboI 552 GATC ^GATC 1050 GATC MboII 831 GAAGA GAAGA(8/7) 650 TCTTC NcoI 23 CCATGG C^CATGG 1170 CCATGG NdeII 552 GATC ^GATC 1050 GATC PleI 731 GACTC GAGTC(4/5) 1231 GAGTC PpuMI 242 GGGACCC [GA]G^G[AT]CC[CT] 500 AGGACCT Sau3AI 552 GATC ^GATC 1050 GATC XcmI 608 CCAAAGACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1170 CCATGGCTTCGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsgI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI MaeIII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIII NlaIV PvuII RsaI Sau96I ScrFI SfaNI SfoI StyI TseI Tsp45I TspRI