(Circular) (Six-base) MAPSORT of: pFN21AB7989.seq Check: 1556 from: 1 to: 6031 pFN21AB7989 6031 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3841 278 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3374 1937 Size: 1437 4594 Acc65I G'GTAC_C Cuts at: 3352 3352 Size: 6031 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4094 5478 5746 1632 Size: 2462 1384 268 1917 Fragments arranged by size: 2462 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5834 AflIII A'CryG_T Cuts at: 1203 4930 5907 1203 Size: 3727 977 1327 Fragments arranged by size: 3727 1327 977 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 4760 4760 Size: 6031 AleI CACnn'nnGTG Cuts at: 1166 2408 1166 Size: 1242 4789 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3050 1184 Size: 1866 4165 AlwNI CAG_nnn'CTG Cuts at: 3774 5346 3774 Size: 1572 4459 ApaI G_GGCC'C Cuts at: 2071 2071 Size: 6031 ApaLI G'TGCA_C Cuts at: 2012 2918 4088 5244 2012 Size: 906 1170 1156 2799 Fragments arranged by size: 2799 1170 1156 906 ApoI r'AATT_y Cuts at: 1493 2150 3340 3566 3718 5827 1493 Size: 657 1190 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 657 226 152 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 2747 2928 3007 3356 1870 Size: 73 804 181 79 349 4545 Fragments arranged by size: 4545 804 349 181 79 73 BamHI G'GATC_C Cuts at: 3361 3728 3361 Size: 367 5664 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2107 2209 2266 2356 3352 Size: 325 217 627 320 102 57 90 996 Cuts at: 3352 4807 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 996 627 325 320 217 102 90 57 BanII G_rGCy'C Cuts at: 1391 2071 2847 3135 3350 1391 Size: 680 776 288 215 4072 Fragments arranged by size: 4072 776 680 288 215 BbeI G_GCGC'C Cuts at: 2111 2213 2270 2111 Size: 102 57 5872 Fragments arranged by size: 5872 102 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2647 928 Size: 971 748 4312 Fragments arranged by size: 4312 971 748 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4222 4256 1903 Size: 34 50 34 2201 34 3678 Fragments arranged by size: 3678 2201 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3923 5133 3923 Size: 1210 4821 BclI T'GATC_A Cuts at: 1548 2556 1548 Size: 1008 5023 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2066 2303 2628 Size: 107 122 71 631 605 394 237 325 Cuts at: 2628 2674 4642 136 Size: 46 1968 1525 Fragments arranged by size: 1968 1525 631 605 394 325 237 122 107 71 46 BglII A'GATC_T Cuts at: 3054 6026 3054 Size: 2972 3059 BlpI GC'TnA_GC Cuts at: 2687 3448 2687 Size: 761 5270 Bme1580I G_kGCm'C Cuts at: 1790 2016 2071 2415 2571 2670 2922 4092 5248 Size: 226 55 344 156 99 252 1170 1156 Cuts at: 5248 1790 Size: 2573 Fragments arranged by size: 2573 1170 1156 344 252 226 156 99 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6031 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3762 4720 442 Size: 416 53 83 399 24 2345 958 1753 Fragments arranged by size: 2345 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 3149 3254 4691 1239 Size: 1283 627 105 1437 2579 Fragments arranged by size: 2579 1437 1283 627 105 Bpu10I CC'TnA_GC Cuts at: 3084 3084 Size: 6031 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3227 4156 5021 5319 3227 Size: 929 865 298 3939 Fragments arranged by size: 3939 929 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4694 882 Size: 617 3195 2219 Fragments arranged by size: 3195 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3822 493 Size: 711 2618 2702 Fragments arranged by size: 2702 2618 711 BsaBI GATnn'nnATC Cuts at: 6025 6025 Size: 6031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2108 2210 2217 2267 Size: 53 83 186 936 575 102 7 50 Cuts at: 2267 3838 4220 4855 275 Size: 1571 382 635 1451 Fragments arranged by size: 1571 1451 936 635 575 382 186 102 83 53 50 7 BsaWI w'CCGG_w Cuts at: 4464 4862 5136 5283 5694 4464 Size: 398 274 147 411 4801 Fragments arranged by size: 4801 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2006 2036 2901 2931 1178 Size: 30 165 30 603 30 865 30 4278 Fragments arranged by size: 4278 865 603 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2280 2351 1186 Size: 1094 71 4866 Fragments arranged by size: 4866 1094 71 BseYI C'CCAG_C Cuts at: 2047 2962 3100 3115 5234 5730 2047 Size: 915 138 15 2119 496 2348 Fragments arranged by size: 2348 2119 915 496 138 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2408 2747 3002 1163 Size: 573 672 339 255 4192 Fragments arranged by size: 4192 672 573 339 255 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2133 2674 3481 4242 5270 1540 Size: 275 186 132 541 807 761 1028 2301 Fragments arranged by size: 2301 1028 807 761 541 275 186 132 BsiHKAI G_wGCw'C Cuts at: 2016 2922 4092 4177 5248 2016 Size: 906 1170 85 1071 2799 Fragments arranged by size: 2799 1170 1071 906 85 BsmI GAATG_Cn' Cuts at: 3542 3635 3542 Size: 93 5938 BsmBI CGTCTCn'nnnn_ Cuts at: 2085 2945 2085 Size: 860 5171 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2071 2415 2571 2670 2847 2922 Size: 399 226 55 344 156 99 177 75 Cuts at: 2922 3135 3350 4092 4177 5248 1391 Size: 213 215 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 344 226 215 213 177 156 99 85 75 55 BspEI T'CCGG_A Cuts at: 5694 5694 Size: 6031 BspHI T'CATG_A Cuts at: 3920 5650 3920 Size: 1730 4301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 2859 3386 844 Size: 1673 342 527 3489 Fragments arranged by size: 3489 1673 527 342 BsrBI CCG'CTC Cuts at: 1394 1823 2116 3093 3918 1394 Size: 429 293 977 825 3507 Fragments arranged by size: 3507 977 825 429 293 BsrDI GCAATG_nn' Cuts at: 65 1248 2523 4526 4700 65 Size: 1183 1275 2003 174 1396 Fragments arranged by size: 2003 1396 1275 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 2104 2128 2673 2811 4675 4862 Size: 110 250 44 24 545 138 1864 187 Cuts at: 4862 1700 Size: 2869 Fragments arranged by size: 2869 1864 545 250 187 138 110 44 24 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6031 BssHII G'CGCG_C Cuts at: 1925 2442 1925 Size: 517 5514 BssSI C'ACGA_G Cuts at: 2333 4091 5103 2333 Size: 1758 1012 3261 Fragments arranged by size: 3261 1758 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6031 BstBI TT'CG_AA Cuts at: 4835 4835 Size: 6031 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6031 BstYI r'GATC_y Cuts at: 1972 3054 3361 3728 4113 4130 5582 5668 5680 Size: 1082 307 367 385 17 1452 86 12 Cuts at: 5680 6026 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1082 385 367 346 307 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2292 2292 Size: 6031 BtgI C'CryG_G Cuts at: 513 1065 2028 3175 513 Size: 552 963 1147 3369 Fragments arranged by size: 3369 1147 963 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5292 Fragments arranged by size: 5292 683 56 BtsI GCAGTG_nn' Cuts at: 2403 2681 3210 3277 3286 3547 4341 4361 2403 Size: 278 529 67 9 261 794 20 4073 Fragments arranged by size: 4073 794 529 278 261 67 20 9 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5996 DraI TTT'AAA Cuts at: 3335 3335 Size: 6031 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5038 809 Size: 507 3722 1802 Fragments arranged by size: 3722 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2433 2610 Size: 54 1425 50 275 18 300 303 177 Cuts at: 2610 2671 2860 3478 4367 5996 8 Size: 61 189 618 889 1629 43 Fragments arranged by size: 1629 1425 889 618 303 300 275 189 177 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2130 2671 3478 1537 Size: 275 318 541 807 4090 Fragments arranged by size: 4090 807 541 318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3961 1353 Size: 2608 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2014 4618 4992 5138 387 Size: 1064 138 425 2604 374 146 1280 Fragments arranged by size: 2604 1280 1064 425 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 3149 3254 4094 4691 5478 5746 Size: 393 890 627 105 840 597 787 268 Cuts at: 5746 1239 Size: 1524 Fragments arranged by size: 1524 890 840 787 627 597 393 268 105 EcoO109I rG'GnC_Cy Cuts at: 2067 2248 2506 2067 Size: 181 258 5592 Fragments arranged by size: 5592 258 181 EcoRI G'AATT_C Cuts at: 2150 3340 3718 2150 Size: 1190 378 4463 Fragments arranged by size: 4463 1190 378 FspI TGC'GCA Cuts at: 4537 4537 Size: 6031 HaeII r_GCGC'y Cuts at: 1678 2038 2111 2213 2270 5178 1678 Size: 360 73 102 57 2908 2531 Fragments arranged by size: 2908 2531 360 102 73 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4672 4704 4746 4778 1373 Size: 32 135 32 3100 32 42 32 2626 Fragments arranged by size: 3100 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3375 3621 669 Size: 988 281 1437 246 3079 Fragments arranged by size: 3079 1437 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6031 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KasI G'GCGC_C Cuts at: 2107 2209 2266 2107 Size: 102 57 5872 Fragments arranged by size: 5872 102 57 KpnI G_GTAC'C Cuts at: 3356 3356 Size: 6031 MfeI C'AATT_G Cuts at: 3630 3630 Size: 6031 MluI A'CGCG_T Cuts at: 5907 5907 Size: 6031 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5145 5329 1305 Size: 3840 184 2007 Fragments arranged by size: 3840 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2612 10 Size: 54 1425 343 780 3429 Fragments arranged by size: 3429 1425 780 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2408 3989 4348 4507 518 Size: 648 843 399 1581 359 159 2042 Fragments arranged by size: 2042 1581 843 648 399 359 159 MspA1I CmG'CkG Cuts at: 1606 2057 2141 2194 2731 2858 2975 3100 3447 Size: 451 84 53 537 127 117 125 347 Cuts at: 3447 4124 5272 5517 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 537 451 347 245 127 125 117 84 53 NaeI GCC'GGC Cuts at: 2062 2106 2130 2675 2062 Size: 44 24 545 5418 Fragments arranged by size: 5418 545 44 24 NarI GG'CG_CC Cuts at: 2108 2210 2267 2108 Size: 102 57 5872 Fragments arranged by size: 5872 102 57 NcoI C'CATG_G Cuts at: 513 1065 2028 3175 513 Size: 552 963 1147 3369 Fragments arranged by size: 3369 1147 963 552 NdeI CA'TA_TG Cuts at: 387 2254 387 Size: 1867 4164 NgoMIV G'CCGG_C Cuts at: 2060 2104 2128 2673 2060 Size: 44 24 545 5418 Fragments arranged by size: 5418 545 44 24 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6031 NotI GC'GGCC_GC Cuts at: 3478 3478 Size: 6031 NspI r_CATG'y Cuts at: 1132 1229 3037 3389 4934 1132 Size: 97 1808 352 1545 2229 Fragments arranged by size: 2229 1808 1545 352 97 PasI CC'CwG_GG Cuts at: 1348 2374 1348 Size: 1026 5005 PciI A'CATG_T Cuts at: 4930 4930 Size: 6031 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6031 PfoI T'CCnGG_A Cuts at: 3126 3126 Size: 6031 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2942 2974 4100 4132 5613 5645 2942 Size: 32 1126 32 1481 32 3328 Fragments arranged by size: 3328 1481 1126 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 2506 2248 Size: 258 5773 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6031 PsiI TTA'TAA Cuts at: 3601 3601 Size: 6031 PspOMI G'GGCC_C Cuts at: 2067 2067 Size: 6031 PstI C_TGCA'G Cuts at: 830 2514 3383 830 Size: 1684 869 3478 Fragments arranged by size: 3478 1684 869 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6031 PvuII CAG'CTG Cuts at: 2057 2141 2858 2975 2057 Size: 84 717 117 5113 Fragments arranged by size: 5113 717 117 84 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SanDI GG'GwC_CC Cuts at: 2248 2248 Size: 6031 SbfI CC_TGCA'GG Cuts at: 2514 3383 2514 Size: 869 5162 ScaI AGT'ACT Cuts at: 4279 5991 4279 Size: 1712 4319 SfcI C'TryA_G Cuts at: 826 1045 2510 3379 4514 5195 5386 826 Size: 219 1465 869 1135 681 191 1471 Fragments arranged by size: 1471 1465 1135 869 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2303 2303 Size: 6031 SfoI GGC'GCC Cuts at: 2109 2211 2268 2109 Size: 102 57 5872 Fragments arranged by size: 5872 102 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6031 SmaI CCC'GGG Cuts at: 1872 3009 3358 1872 Size: 1137 349 4545 Fragments arranged by size: 4545 1137 349 SmlI C'TyrA_G Cuts at: 820 1017 1943 3242 4135 5036 5298 820 Size: 197 926 1299 893 901 262 1553 Fragments arranged by size: 1553 1299 926 901 893 262 197 SnaBI TAC'GTA Cuts at: 493 3822 493 Size: 3329 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6031 SphI G_CATG'C Cuts at: 1132 3037 3389 1132 Size: 1905 352 3774 Fragments arranged by size: 3774 1905 352 SspI AAT'ATT Cuts at: 5 52 3955 5 Size: 47 3903 2081 Fragments arranged by size: 3903 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6031 StyI C'CwwG_G Cuts at: 513 1065 2028 2607 2967 3175 3297 3470 513 Size: 552 963 579 360 208 122 173 3074 Fragments arranged by size: 3074 963 579 552 360 208 173 122 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3188 4058 4075 4228 4413 4858 325 Size: 1255 1608 870 17 153 185 445 1498 Fragments arranged by size: 1608 1498 1255 870 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2703 3139 4277 Size: 274 80 33 51 1443 726 436 1138 Cuts at: 4277 5989 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1138 726 436 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2006 2038 2901 2933 5991 6023 2006 Size: 32 863 32 3058 32 2014 Fragments arranged by size: 3058 2014 863 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6031 XbaI T'CTAG_A Cuts at: 3367 3367 Size: 6031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2620 3182 2620 Size: 562 5469 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 3007 3356 1870 Size: 1137 349 4545 Fragments arranged by size: 4545 1137 349 XmnI GAAnn'nnTTC Cuts at: 4160 4160 Size: 6031 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3839 276 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SrfI SwaI