(Linear) (Six-base) MAPSORT of: KIBB7989.seq Check: 2139 from: 1 to: 1326 KIBB7989.orf With 182 enzymes: SgfI * July 3, 2012 16:32 .. AleI CACnn'nnGTG Cuts at: 0 403 1326 Size: 403 923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1045 1326 Size: 1045 281 ApaI G_GGCC'C Cuts at: 0 66 1326 Size: 66 1260 ApaLI G'TGCA_C Cuts at: 0 7 913 1326 Size: 7 906 413 Fragments arranged by size: 906 413 7 ApoI r'AATT_y Cuts at: 0 145 1326 Size: 145 1181 AvaI C'yCGr_G Cuts at: 0 742 923 1002 1326 Size: 742 181 79 324 Fragments arranged by size: 742 324 181 79 BanI G'GyrC_C Cuts at: 0 102 204 261 351 1326 Size: 102 102 57 90 975 Fragments arranged by size: 975 102 102 90 57 BanII G_rGCy'C Cuts at: 0 66 842 1130 1326 Size: 66 776 288 196 Fragments arranged by size: 776 288 196 66 BbeI G_GCGC'C Cuts at: 0 106 208 265 1326 Size: 106 102 57 1061 Fragments arranged by size: 1061 106 102 57 BbsI GAAGACnn'nnnn_ Cuts at: 0 642 1326 Size: 642 684 BclI T'GATC_A Cuts at: 0 551 1326 Size: 551 775 BglI GCCn_nnn'nGGC Cuts at: 0 61 298 623 669 1326 Size: 61 237 325 46 657 Fragments arranged by size: 657 325 237 61 46 BglII A'GATC_T Cuts at: 0 1049 1326 Size: 1049 277 BlpI GC'TnA_GC Cuts at: 0 682 1326 Size: 682 644 Bme1580I G_kGCm'C Cuts at: 0 11 66 410 566 665 917 1326 Size: 11 55 344 156 99 252 409 Fragments arranged by size: 409 344 252 156 99 55 11 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 517 1144 1249 1326 Size: 517 627 105 77 Fragments arranged by size: 627 517 105 77 Bpu10I CC'TnA_GC Cuts at: 0 1079 1326 Size: 1079 247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1222 1326 Size: 1222 104 BsaHI Gr'CG_yC Cuts at: 0 103 205 212 262 1326 Size: 103 102 7 50 1064 Fragments arranged by size: 1064 103 102 50 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1 31 896 926 1326 Size: 1 30 865 30 400 Fragments arranged by size: 865 400 30 30 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 275 346 1326 Size: 275 71 980 Fragments arranged by size: 980 275 71 BseYI C'CCAG_C Cuts at: 0 42 957 1095 1110 1326 Size: 42 915 138 15 216 Fragments arranged by size: 915 216 138 42 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 742 997 1326 Size: 403 339 255 329 Fragments arranged by size: 403 339 329 255 BsiEI CG_ry'CG Cuts at: 0 128 669 1326 Size: 128 541 657 Fragments arranged by size: 657 541 128 BsiHKAI G_wGCw'C Cuts at: 0 11 917 1326 Size: 11 906 409 Fragments arranged by size: 906 409 11 BsmBI CGTCTCn'nnnn_ Cuts at: 0 80 940 1326 Size: 80 860 386 Fragments arranged by size: 860 386 80 Bsp1286I G_dGCh'C Cuts at: 0 11 66 410 566 665 842 917 1130 Size: 11 55 344 156 99 177 75 213 Cuts at: 1130 1326 Size: 196 Fragments arranged by size: 344 213 196 177 156 99 75 55 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 512 854 1326 Size: 512 342 472 Fragments arranged by size: 512 472 342 BsrBI CCG'CTC Cuts at: 0 111 1088 1326 Size: 111 977 238 Fragments arranged by size: 977 238 111 BsrDI GCAATG_nn' Cuts at: 0 518 1326 Size: 518 808 BsrFI r'CCGG_y Cuts at: 0 55 99 123 668 806 1326 Size: 55 44 24 545 138 520 Fragments arranged by size: 545 520 138 55 44 24 BssHII G'CGCG_C Cuts at: 0 437 1326 Size: 437 889 BssSI C'ACGA_G Cuts at: 0 328 1326 Size: 328 998 BstYI r'GATC_y Cuts at: 0 1049 1326 Size: 1049 277 Bsu36I CC'TnA_GG Cuts at: 0 287 1326 Size: 287 1039 BtgI C'CryG_G Cuts at: 0 23 1170 1326 Size: 23 1147 156 Fragments arranged by size: 1147 156 23 BtsI GCAGTG_nn' Cuts at: 0 398 676 1205 1272 1281 1326 Size: 398 278 529 67 9 45 Fragments arranged by size: 529 398 278 67 45 9 EaeI y'GGCC_r Cuts at: 0 125 428 605 666 855 1326 Size: 125 303 177 61 189 471 Fragments arranged by size: 471 303 189 177 125 61 EagI C'GGCC_G Cuts at: 0 125 666 1326 Size: 125 541 660 Fragments arranged by size: 660 541 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 9 1326 Size: 9 1317 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 517 1144 1249 1326 Size: 517 627 105 77 Fragments arranged by size: 627 517 105 77 EcoO109I rG'GnC_Cy Cuts at: 0 62 243 501 1326 Size: 62 181 258 825 Fragments arranged by size: 825 258 181 62 EcoRI G'AATT_C Cuts at: 0 145 1326 Size: 145 1181 HaeII r_GCGC'y Cuts at: 0 33 106 208 265 1326 Size: 33 73 102 57 1061 Fragments arranged by size: 1061 102 73 57 33 KasI G'GCGC_C Cuts at: 0 102 204 261 1326 Size: 102 102 57 1065 Fragments arranged by size: 1065 102 102 57 MscI TGG'CCA Cuts at: 0 607 1326 Size: 607 719 MslI CAynn'nnrTG Cuts at: 0 403 1326 Size: 403 923 MspA1I CmG'CkG Cuts at: 0 52 136 189 726 853 970 1095 1326 Size: 52 84 53 537 127 117 125 231 Fragments arranged by size: 537 231 127 125 117 84 53 52 NaeI GCC'GGC Cuts at: 0 57 101 125 670 1326 Size: 57 44 24 545 656 Fragments arranged by size: 656 545 57 44 24 NarI GG'CG_CC Cuts at: 0 103 205 262 1326 Size: 103 102 57 1064 Fragments arranged by size: 1064 103 102 57 NcoI C'CATG_G Cuts at: 0 23 1170 1326 Size: 23 1147 156 Fragments arranged by size: 1147 156 23 NdeI CA'TA_TG Cuts at: 0 249 1326 Size: 249 1077 NgoMIV G'CCGG_C Cuts at: 0 55 99 123 668 1326 Size: 55 44 24 545 658 Fragments arranged by size: 658 545 55 44 24 NspI r_CATG'y Cuts at: 0 1032 1326 Size: 1032 294 PasI CC'CwG_GG Cuts at: 0 369 1326 Size: 369 957 PfoI T'CCnGG_A Cuts at: 0 1121 1326 Size: 1121 205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 937 969 1326 Size: 937 32 357 Fragments arranged by size: 937 357 32 PpuMI rG'GwC_Cy Cuts at: 0 243 501 1326 Size: 243 258 825 Fragments arranged by size: 825 258 243 PspOMI G'GGCC_C Cuts at: 0 62 1326 Size: 62 1264 PstI C_TGCA'G Cuts at: 0 509 1326 Size: 509 817 PvuII CAG'CTG Cuts at: 0 52 136 853 970 1326 Size: 52 84 717 117 356 Fragments arranged by size: 717 356 117 84 52 SanDI GG'GwC_CC Cuts at: 0 243 1326 Size: 243 1083 SbfI CC_TGCA'GG Cuts at: 0 509 1326 Size: 509 817 SfcI C'TryA_G Cuts at: 0 505 1326 Size: 505 821 SfiI GGCCn_nnn'nGGCC Cuts at: 0 298 1326 Size: 298 1028 SfoI GGC'GCC Cuts at: 0 104 206 263 1326 Size: 104 102 57 1063 Fragments arranged by size: 1063 104 102 57 SmaI CCC'GGG Cuts at: 0 1004 1326 Size: 1004 322 SmlI C'TyrA_G Cuts at: 0 1237 1326 Size: 1237 89 SphI G_CATG'C Cuts at: 0 1032 1326 Size: 1032 294 StyI C'CwwG_G Cuts at: 0 23 602 962 1170 1292 1326 Size: 23 579 360 208 122 34 Fragments arranged by size: 579 360 208 122 34 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1183 1326 Size: 1183 143 TatI w'GTAC_w Cuts at: 0 698 1134 1326 Size: 698 436 192 Fragments arranged by size: 698 436 192 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1 33 896 928 1326 Size: 1 32 863 32 398 Fragments arranged by size: 863 398 32 32 1 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 615 1177 1326 Size: 615 562 149 Fragments arranged by size: 615 562 149 XmaI C'CCGG_G Cuts at: 0 1002 1326 Size: 1002 324 Enzymes that do cut: AleI AlfI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BclI BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstYI Bsu36I BtgI BtsI EaeI EagI EciI Eco57MI EcoO109I EcoRI HaeII KasI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PfoI PpiI PpuMI PspOMI PstI PvuII SanDI SbfI SfcI SfiI SfoI SmaI SmlI SphI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AloI AlwNI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrGI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI