################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII AccII AclI AcyI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BsmBI BsoBI BspLU11I BsrBI BsrFI BssHII BssSI BstAPI BstUI BstXI CelII Cfr10I CpoI DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I EcoRV FbaI FseI FspI Hin1I HincII HindII HpaI KasI KspI MamI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SphI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 2915 GGTACC G^GTACC AccIII 1463 TCCGGA T^CCGGA AhdI 2788 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 837 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2915 GGTACC G^GTACC AspEI 2788 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2059 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 2915 GGTACC G^G[CT][GA]CC BglII 346 AGATCT A^GATCT BsaAI 2150 TACGTA [CT]AC^GT[GA] BsaI 1788 GAGACC GGTCTC(1/5) BseAI 1463 TCCGGA T^CCGGA BseRI 282 GAGGAG GAGGAG(10/8) BsgI 206 GTGCAG GTGCAG(16/14) BsmFI 1584 GGGAC GGGAC(10/14) BsmI 2177 GAATGC GAATGC(1/-1) Bsp1407I 2759 TGTACA T^GTACA BspDI 1550 ATCGAT AT^CGAT BspEI 1463 TCCGGA T^CCGGA BspHI 1344 TCATGA T^CATGA BsrDI 1333 GCAATG GCAATG(2/0) BsrGI 2759 TGTACA T^GTACA Bst1107I 17 GTATAC GTA^TAC BstBI 299 TTCGAA TT^CGAA BstEII 2231 GGTTACC G^GT[ACGT]ACC BstPI 2231 GGTTACC G^GT[ACGT]ACC BstZ17I 17 GTATAC GTA^TAC CfoI 1521 GCGC GCG^C ClaI 1550 ATCGAT AT^CGAT DraI 979 TTTAAA TTT^AAA DraII 419 AGGACCT [GA]G^G[ACGT]CC[CT] Eam1105I 2788 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 419 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 2231 GGTTACC G^GT[ACGT]ACC EcoRI 2539 GAATTC G^AATTC EcoT22I 310 ATGCAT ATGCA^T FauI 1353 CCCGC CCCGC(4/6) HaeII 1520 GGCGCT [GA]GCGC^[CT] HhaI 1521 GCGC GCG^C HinP1I 1521 GCGC G^CGC KpnI 2915 GGTACC GGTAC^C MaeII 2151 ACGT A^CGT MroI 1463 TCCGGA T^CCGGA MslI 2172 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NsiI 310 ATGCAT ATGCA^T NspV 299 TTCGAA TT^CGAA PflFI 2059 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1681 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 419 AGGACCT [GA]G^G[AT]CC[CT] PshAI 2767 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2660 CTGCAG CTGCA^G RcaI 1344 TCATGA T^CATGA ScaI 2439 AGTACT AGT^ACT SfuI 299 TTCGAA TT^CGAA SnaBI 2150 TACGTA TAC^GTA SpeI 1089 ACTAGT A^CTAGT SspBI 2759 TGTACA T^GTACA SspI 1693 AATATT AAT^ATT StuI 2397 AGGCCT AGG^CCT Tth111I 2059 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1681 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 837 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwNI 1358 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG 2855 CAGCCACTG AocI 1719 CCTGAGG CC^T[ACGT]AGG 2867 CCTTAGG BcnI 202 CCCGG CC^[GC]GG 1582 CCGGG BsaWI 696 TCCGGT [AT]^CCGG[AT] 1463 TCCGGA Bsu36I 1719 CCTGAGG CC^T[ACGT]AGG 2867 CCTTAGG Cac8I 262 GCAAGC GC[ACGT]^[ACGT]GC 2290 GCAAGC Eco81I 1719 CCTGAGG CC^T[ACGT]AGG 2867 CCTTAGG EcoNI 364 CCTTACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1715 CCTCCCTGAGG EcoT14I 141 CCTTGG C^C[AT][AT]GG 2295 CCTTGG HgaI 1360 GACGC GACGC(5/10) 2772 GCGTC NciI 202 CCCGG CC^[GC]GG 1582 CCGGG NspI 176 ACATGC [GA]CATG^[CT] 500 ACATGC StyI 141 CCTTGG C^C[AT][AT]GG 2295 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII