(Circular) (Six-base) MAPSORT of: pFN21AE4258.seq Check: 3092 from: 1 to: 7648 pFN21AE4258 7648 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2204 2204 Size: 7648 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5458 278 Size: 53 83 186 936 3922 2468 Fragments arranged by size: 3922 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2023 3194 4791 4991 1937 Size: 86 1171 1597 200 4594 Fragments arranged by size: 4594 1597 1171 200 86 Acc65I G'GTAC_C Cuts at: 4920 4969 4920 Size: 49 7599 AclI AA'CG_TT Cuts at: 1563 5775 6148 1563 Size: 4212 373 3063 Fragments arranged by size: 4212 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2535 4383 4497 5711 7095 7363 1632 Size: 903 1848 114 1214 1384 268 1917 Fragments arranged by size: 1917 1848 1384 1214 903 268 114 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7451 AflIII A'CryG_T Cuts at: 1203 6547 7524 1203 Size: 5344 977 1327 Fragments arranged by size: 5344 1327 977 AgeI A'CCGG_T Cuts at: 6479 6479 Size: 7648 AhdI GACnn_n'nnGTC Cuts at: 4798 6377 4798 Size: 1579 6069 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7648 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2731 1184 Size: 1547 6101 AlwNI CAG_nnn'CTG Cuts at: 3368 4865 5391 6963 3368 Size: 1497 526 1572 4053 Fragments arranged by size: 4053 1572 1497 526 ApaLI G'TGCA_C Cuts at: 5705 6861 5705 Size: 1156 6492 ApoI r'AATT_y Cuts at: 1493 2649 2915 3184 4544 4629 4957 5183 5335 Size: 1156 266 269 1360 85 328 226 152 Cuts at: 5335 7444 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1360 1156 328 269 266 226 152 85 AseI AT'TA_AT Cuts at: 160 1032 6202 160 Size: 872 5170 1606 Fragments arranged by size: 5170 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7648 AvaI C'yCGr_G Cuts at: 1870 1943 4973 1870 Size: 73 3030 4545 Fragments arranged by size: 4545 3030 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4904 4912 4937 4945 4904 Size: 8 25 8 7607 Fragments arranged by size: 7607 25 8 8 BamHI G'GATC_C Cuts at: 4978 5345 4978 Size: 367 7281 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4920 4969 6424 618 Size: 325 217 627 3133 49 1455 1842 Fragments arranged by size: 3133 1842 1455 627 325 217 49 BanII G_rGCy'C Cuts at: 1391 2087 3046 3330 4020 4967 1391 Size: 696 959 284 690 947 4072 Fragments arranged by size: 4072 959 947 696 690 284 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2332 3510 4122 928 Size: 971 433 1178 612 4454 Fragments arranged by size: 4454 1178 971 612 433 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5839 5873 1903 Size: 34 3902 34 3678 Fragments arranged by size: 3902 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5540 6750 5540 Size: 1210 6438 BclI T'GATC_A Cuts at: 1548 1548 Size: 7648 BfrBI ATG'CAT Cuts at: 2317 2317 Size: 7648 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6259 136 Size: 107 122 71 631 605 4587 1525 Fragments arranged by size: 4587 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2351 7643 2351 Size: 5292 2356 BlpI GC'TnA_GC Cuts at: 5065 5065 Size: 7648 Bme1580I G_kGCm'C Cuts at: 1790 5709 6865 1790 Size: 3919 1156 2573 Fragments arranged by size: 3919 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7648 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4091 4457 4558 Size: 416 53 83 399 24 2674 366 101 Cuts at: 4558 4920 5379 6337 442 Size: 362 459 958 1753 Fragments arranged by size: 2674 1753 958 459 416 399 366 362 101 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7648 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2858 2898 3154 4214 6308 1239 Size: 1619 40 256 1060 2094 2579 Fragments arranged by size: 2579 2094 1619 1060 256 40 Bpu10I CC'TnA_GC Cuts at: 3762 4455 3762 Size: 693 6955 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3677 3719 3976 5773 6638 6936 3677 Size: 42 257 1797 865 298 4389 Fragments arranged by size: 4389 1797 865 298 257 42 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3787 6311 882 Size: 617 2288 2524 2219 Fragments arranged by size: 2524 2288 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4157 5439 493 Size: 711 2953 1282 2702 Fragments arranged by size: 2953 2702 1282 711 BsaBI GATnn'nnATC Cuts at: 7642 7642 Size: 7648 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5455 5837 6472 275 Size: 53 83 186 936 3922 382 635 1451 Fragments arranged by size: 3922 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2701 3468 6081 6479 6753 6900 7311 2701 Size: 767 2613 398 274 147 411 3038 Fragments arranged by size: 3038 2613 767 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 1178 Size: 30 165 30 687 30 6706 Fragments arranged by size: 6706 687 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2302 1186 Size: 1116 6532 BseYI C'CCAG_C Cuts at: 2658 4095 6851 7347 2658 Size: 1437 2756 496 2959 Fragments arranged by size: 2959 2756 1437 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2232 1163 Size: 573 496 6579 Fragments arranged by size: 6579 573 496 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5098 5859 6887 1540 Size: 275 186 3097 761 1028 2301 Fragments arranged by size: 3097 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3046 3330 4020 4456 5709 5794 6865 3046 Size: 284 690 436 1253 85 1071 3829 Fragments arranged by size: 3829 1253 1071 690 436 284 85 BsmI GAATG_Cn' Cuts at: 4188 5159 5252 4188 Size: 971 93 6584 Fragments arranged by size: 6584 971 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 3046 3330 4020 4456 4967 5709 Size: 399 297 959 284 690 436 511 742 Cuts at: 5709 5794 6865 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 959 742 690 511 436 399 297 284 85 BspEI T'CCGG_A Cuts at: 3468 7311 3468 Size: 3843 3805 BspHI T'CATG_A Cuts at: 3349 5537 7267 3349 Size: 2188 1730 3730 Fragments arranged by size: 3730 2188 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2204 2573 2633 5003 844 Size: 1360 369 60 2370 3489 Fragments arranged by size: 3489 2370 1360 369 60 BsrBI CCG'CTC Cuts at: 1394 1823 5535 1394 Size: 429 3712 3507 Fragments arranged by size: 3712 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3345 6143 6317 65 Size: 1183 2097 2798 174 1396 Fragments arranged by size: 2798 2097 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6292 6479 1700 Size: 110 4482 187 2869 Fragments arranged by size: 4482 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4764 96 Size: 4668 2980 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7648 BssSI C'ACGA_G Cuts at: 5708 6720 5708 Size: 1012 6636 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7648 BstBI TT'CG_AA Cuts at: 2305 6452 2305 Size: 4147 3501 BstEII G'GTnAC_C Cuts at: 1184 4236 1184 Size: 3052 4596 BstYI r'GATC_y Cuts at: 1972 2351 3471 4170 4719 4978 5345 5730 5747 Size: 379 1120 699 549 259 367 385 17 Cuts at: 5747 7199 7285 7297 7643 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1120 699 549 385 379 367 346 259 86 17 12 BstZ17I GTA'TAC Cuts at: 2024 2024 Size: 7648 Bsu36I CC'TnA_GG Cuts at: 3725 4873 3725 Size: 1148 6500 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7096 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6909 Fragments arranged by size: 6909 683 56 BtsI GCAGTG_nn' Cuts at: 4526 4674 4863 5164 5958 5978 4526 Size: 148 189 301 794 20 6196 Fragments arranged by size: 6196 794 301 189 148 20 ClaI AT'CG_AT Cuts at: 1554 3556 1554 Size: 2002 5646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2076 2111 4650 4685 529 Size: 35 1512 35 2539 35 3492 Fragments arranged by size: 3492 2539 1512 35 35 35 DraI TTT'AAA Cuts at: 2986 4952 2986 Size: 1966 5682 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6655 809 Size: 507 5339 1802 Fragments arranged by size: 5339 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5095 5984 7613 Size: 54 1425 50 275 18 3265 889 1629 Cuts at: 7613 8 Size: 43 Fragments arranged by size: 3265 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5095 1537 Size: 275 3283 4090 Fragments arranged by size: 4090 3283 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2405 2600 4003 5578 1353 Size: 1052 195 1403 1575 3423 Fragments arranged by size: 3423 1575 1403 1052 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6235 6609 6755 387 Size: 1064 138 4646 374 146 1280 Fragments arranged by size: 4646 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3044 3328 4018 3044 Size: 284 690 6674 Fragments arranged by size: 6674 690 284 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2535 2858 2898 3154 4214 4383 4497 Size: 393 903 323 40 256 1060 169 114 Cuts at: 4497 5711 6308 7095 7363 1239 Size: 1214 597 787 268 1524 Fragments arranged by size: 1524 1214 1060 903 787 597 393 323 268 256 169 114 40 EcoNI CCTnn'n_nnAGG Cuts at: 2373 3724 2373 Size: 1351 6297 EcoO109I rG'GnC_Cy Cuts at: 2425 2425 Size: 7648 EcoRI G'AATT_C Cuts at: 4544 4957 5335 4544 Size: 413 378 6857 Fragments arranged by size: 6857 413 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2734 2734 Size: 7648 FspI TGC'GCA Cuts at: 6154 6154 Size: 7648 HaeII r_GCGC'y Cuts at: 1678 3529 6795 1678 Size: 1851 3266 2531 Fragments arranged by size: 3266 2531 1851 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2341 2373 2620 2652 2871 Size: 32 135 32 769 32 247 32 219 Cuts at: 2871 2903 2981 3013 3908 3940 3978 4010 4262 Size: 32 78 32 895 32 38 32 252 Cuts at: 4262 4294 4784 4816 6289 6321 6363 6395 1373 Size: 32 490 32 1473 32 42 32 2626 Fragments arranged by size: 2626 1473 895 769 490 252 247 219 135 78 42 38 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4992 5238 669 Size: 988 281 3054 246 3079 Fragments arranged by size: 3079 3054 988 281 246 HindIII A'AGCT_T Cuts at: 748 2269 2300 3159 3752 748 Size: 1521 31 859 593 4644 Fragments arranged by size: 4644 1521 859 593 31 HpaI GTT'AAC Cuts at: 5238 5238 Size: 7648 KpnI G_GTAC'C Cuts at: 4924 4973 4924 Size: 49 7599 MfeI C'AATT_G Cuts at: 5247 5247 Size: 7648 MluI A'CGCG_T Cuts at: 7524 7524 Size: 7648 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2076 3019 6762 6946 1305 Size: 771 943 3743 184 2007 Fragments arranged by size: 3743 2007 943 771 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5826 Fragments arranged by size: 5826 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4181 5606 5965 6124 518 Size: 648 3015 1425 359 159 2042 Fragments arranged by size: 3015 2042 1425 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2402 2836 3301 3544 4798 5064 5741 6889 Size: 796 434 465 243 1254 266 677 1148 Cuts at: 6889 7134 1606 Size: 245 2120 Fragments arranged by size: 2120 1254 1148 796 677 465 434 266 245 243 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7096 NdeI CA'TA_TG Cuts at: 387 387 Size: 7648 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7648 NotI GC'GGCC_GC Cuts at: 5095 5095 Size: 7648 NsiI A_TGCA'T Cuts at: 2319 2319 Size: 7648 NspI r_CATG'y Cuts at: 1132 1229 2185 2509 5006 6551 1132 Size: 97 956 324 2497 1545 2229 Fragments arranged by size: 2497 2229 1545 956 324 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7648 PciI A'CATG_T Cuts at: 6547 6547 Size: 7648 PflMI CCAn_nnn'nTGG Cuts at: 1108 3692 1108 Size: 2584 5064 PfoI T'CCnGG_A Cuts at: 4901 4901 Size: 7648 PmeI GTTT'AAAC Cuts at: 4952 4952 Size: 7648 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5717 5749 7230 7262 5717 Size: 32 1481 32 6103 Fragments arranged by size: 6103 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2425 2425 Size: 7648 PshAI GACnn'nnGTC Cuts at: 1613 4776 1613 Size: 3163 4485 PsiI TTA'TAA Cuts at: 5218 5218 Size: 7648 PstI C_TGCA'G Cuts at: 830 4669 5000 830 Size: 3839 331 3478 Fragments arranged by size: 3839 3478 331 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7648 PvuII CAG'CTG Cuts at: 2402 2836 3301 3544 4798 2402 Size: 434 465 243 1254 5252 Fragments arranged by size: 5252 1254 465 434 243 SacI G_AGCT'C Cuts at: 3046 3330 4020 3046 Size: 284 690 6674 Fragments arranged by size: 6674 690 284 SalI G'TCGA_C Cuts at: 1936 4990 1936 Size: 3054 4594 SbfI CC_TGCA'GG Cuts at: 5000 5000 Size: 7648 ScaI AGT'ACT Cuts at: 4446 5896 7608 4446 Size: 1450 1712 4486 Fragments arranged by size: 4486 1712 1450 SfcI C'TryA_G Cuts at: 826 1045 2565 3475 4244 4665 4788 4996 6131 Size: 219 1520 910 769 421 123 208 1135 Cuts at: 6131 6812 7003 826 Size: 681 191 1471 Fragments arranged by size: 1520 1471 1135 910 769 681 421 219 208 191 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7648 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7648 SmaI CCC'GGG Cuts at: 1872 4975 1872 Size: 3103 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3656 3734 3955 5752 6653 6915 Size: 197 926 1713 78 221 1797 901 262 Cuts at: 6915 820 Size: 1553 Fragments arranged by size: 1797 1713 1553 926 901 262 221 197 78 SnaBI TAC'GTA Cuts at: 493 4157 5439 493 Size: 3664 1282 2702 Fragments arranged by size: 3664 2702 1282 SpeI A'CTAG_T Cuts at: 152 3094 152 Size: 2942 4706 SphI G_CATG'C Cuts at: 1132 5006 1132 Size: 3874 3774 SspI AAT'ATT Cuts at: 5 52 3700 5572 5 Size: 47 3648 1872 2081 Fragments arranged by size: 3648 2081 1872 47 StuI AGG'CCT Cuts at: 1517 4404 1517 Size: 2887 4761 StyI C'CwwG_G Cuts at: 513 1065 2146 4300 5087 513 Size: 552 1081 2154 787 3074 Fragments arranged by size: 3074 2154 1081 787 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2145 2362 3778 4134 4439 5675 5692 Size: 1255 565 217 1416 356 305 1236 17 Cuts at: 5692 5845 6030 6475 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1416 1255 1236 565 445 356 305 217 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4444 4764 4856 Size: 274 80 33 51 1443 2467 320 92 Cuts at: 4856 5894 7606 96 Size: 1038 1712 138 Fragments arranged by size: 2467 1712 1443 1038 320 274 138 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2127 2159 2683 2715 3611 3643 3739 3771 4704 Size: 32 524 32 896 32 96 32 933 Cuts at: 4704 4736 7608 7640 2127 Size: 32 2872 32 2135 Fragments arranged by size: 2872 2135 933 896 524 96 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4067 1316 Size: 2751 4897 XbaI T'CTAG_A Cuts at: 4984 4984 Size: 7648 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2737 4347 2167 Size: 570 1610 5468 Fragments arranged by size: 5468 1610 570 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7648 XmaI C'CCGG_G Cuts at: 1870 4973 1870 Size: 3103 4545 XmnI GAAnn'nnTTC Cuts at: 2846 5777 2846 Size: 2931 4717 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5456 276 Size: 53 83 186 936 3922 2468 Fragments arranged by size: 3922 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstXI DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI