(Linear) (Six-base) MAPSORT of: KIEE4258.seq Check: 7191 from: 1 to: 2943 KIEE4258.orf With 182 enzymes: SgfI * July 3, 2012 18:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 199 2943 Size: 199 2744 AccI GT'mk_AC Cuts at: 0 18 1189 2786 2943 Size: 18 1171 1597 157 Fragments arranged by size: 1597 1171 157 18 Acc65I G'GTAC_C Cuts at: 0 2915 2943 Size: 2915 28 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 530 2378 2492 2943 Size: 530 1848 114 451 Fragments arranged by size: 1848 530 451 114 AhdI GACnn_n'nnGTC Cuts at: 0 2793 2943 Size: 2793 150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 726 2943 Size: 726 2217 AlwNI CAG_nnn'CTG Cuts at: 0 1363 2860 2943 Size: 1363 1497 83 Fragments arranged by size: 1497 1363 83 ApoI r'AATT_y Cuts at: 0 644 910 1179 2539 2624 2943 Size: 644 266 269 1360 85 319 Fragments arranged by size: 1360 644 319 269 266 85 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2899 2907 2932 2940 2943 Size: 2899 8 25 8 3 Fragments arranged by size: 2899 25 8 8 3 BanI G'GyrC_C Cuts at: 0 2915 2943 Size: 2915 28 BanII G_rGCy'C Cuts at: 0 82 1041 1325 2015 2943 Size: 82 959 284 690 928 Fragments arranged by size: 959 928 690 284 82 BbsI GAAGACnn'nnnn_ Cuts at: 0 327 1505 2117 2943 Size: 327 1178 612 826 Fragments arranged by size: 1178 826 612 327 BfrBI ATG'CAT Cuts at: 0 312 2943 Size: 312 2631 BglII A'GATC_T Cuts at: 0 346 2943 Size: 346 2597 BmrI ACTGGGnnnn_n' Cuts at: 0 2086 2452 2553 2915 2943 Size: 2086 366 101 362 28 Fragments arranged by size: 2086 366 362 101 28 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 853 893 1149 2209 2943 Size: 853 40 256 1060 734 Fragments arranged by size: 1060 853 734 256 40 Bpu10I CC'TnA_GC Cuts at: 0 1757 2450 2943 Size: 1757 693 493 Fragments arranged by size: 1757 693 493 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1672 1714 1971 2943 Size: 1672 42 257 972 Fragments arranged by size: 1672 972 257 42 BsaI GGTCTCn'nnnn_ Cuts at: 0 1782 2943 Size: 1782 1161 BsaAI yAC'GTr Cuts at: 0 2152 2943 Size: 2152 791 BsaWI w'CCGG_w Cuts at: 0 696 1463 2943 Size: 696 767 1480 Fragments arranged by size: 1480 767 696 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 85 115 2943 Size: 85 30 2828 Fragments arranged by size: 2828 85 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 297 2943 Size: 297 2646 BseYI C'CCAG_C Cuts at: 0 653 2090 2943 Size: 653 1437 853 Fragments arranged by size: 1437 853 653 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 227 2943 Size: 227 2716 BsiHKAI G_wGCw'C Cuts at: 0 1041 1325 2015 2451 2943 Size: 1041 284 690 436 492 Fragments arranged by size: 1041 690 492 436 284 BsmI GAATG_Cn' Cuts at: 0 2183 2943 Size: 2183 760 Bsp1286I G_dGCh'C Cuts at: 0 82 1041 1325 2015 2451 2943 Size: 82 959 284 690 436 492 Fragments arranged by size: 959 690 492 436 284 82 BspEI T'CCGG_A Cuts at: 0 1463 2943 Size: 1463 1480 BspHI T'CATG_A Cuts at: 0 1344 2943 Size: 1344 1599 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 199 568 628 2943 Size: 199 369 60 2315 Fragments arranged by size: 2315 369 199 60 BsrDI GCAATG_nn' Cuts at: 0 1340 2943 Size: 1340 1603 BsrGI T'GTAC_A Cuts at: 0 2759 2943 Size: 2759 184 BstBI TT'CG_AA Cuts at: 0 300 2943 Size: 300 2643 BstEII G'GTnAC_C Cuts at: 0 2231 2943 Size: 2231 712 BstYI r'GATC_y Cuts at: 0 346 1466 2165 2714 2943 Size: 346 1120 699 549 229 Fragments arranged by size: 1120 699 549 346 229 BstZ17I GTA'TAC Cuts at: 0 19 2943 Size: 19 2924 Bsu36I CC'TnA_GG Cuts at: 0 1720 2868 2943 Size: 1720 1148 75 Fragments arranged by size: 1720 1148 75 BtsI GCAGTG_nn' Cuts at: 0 2521 2669 2858 2943 Size: 2521 148 189 85 Fragments arranged by size: 2521 189 148 85 ClaI AT'CG_AT Cuts at: 0 1551 2943 Size: 1551 1392 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 71 106 2645 2680 2943 Size: 71 35 2539 35 263 Fragments arranged by size: 2539 263 71 35 35 DraI TTT'AAA Cuts at: 0 981 2943 Size: 981 1962 EarI CTCTTCn'nnn_ Cuts at: 0 400 595 1998 2943 Size: 400 195 1403 945 Fragments arranged by size: 1403 945 400 195 EcoICRI GAG'CTC Cuts at: 0 1039 1323 2013 2943 Size: 1039 284 690 930 Fragments arranged by size: 1039 930 690 284 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 530 853 893 1149 2209 2378 2492 2943 Size: 530 323 40 256 1060 169 114 451 Fragments arranged by size: 1060 530 451 323 256 169 114 40 EcoNI CCTnn'n_nnAGG Cuts at: 0 368 1719 2943 Size: 368 1351 1224 Fragments arranged by size: 1351 1224 368 EcoO109I rG'GnC_Cy Cuts at: 0 420 2943 Size: 420 2523 EcoRI G'AATT_C Cuts at: 0 2539 2943 Size: 2539 404 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 729 2943 Size: 729 2214 HaeII r_GCGC'y Cuts at: 0 1524 2943 Size: 1524 1419 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 336 368 615 647 866 898 976 1008 Size: 336 32 247 32 219 32 78 32 Cuts at: 1008 1903 1935 1973 2005 2257 2289 2779 2811 Size: 895 32 38 32 252 32 490 32 Cuts at: 2811 2943 Size: 132 Fragments arranged by size: 895 490 336 252 247 219 132 78 38 32 32 32 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 264 295 1154 1747 2943 Size: 264 31 859 593 1196 Fragments arranged by size: 1196 859 593 264 31 KpnI G_GTAC'C Cuts at: 0 2919 2943 Size: 2919 24 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 71 1014 2943 Size: 71 943 1929 Fragments arranged by size: 1929 943 71 MslI CAynn'nnrTG Cuts at: 0 2176 2943 Size: 2176 767 MspA1I CmG'CkG Cuts at: 0 397 831 1296 1539 2793 2943 Size: 397 434 465 243 1254 150 Fragments arranged by size: 1254 465 434 397 243 150 NsiI A_TGCA'T Cuts at: 0 314 2943 Size: 314 2629 NspI r_CATG'y Cuts at: 0 180 504 2943 Size: 180 324 2439 Fragments arranged by size: 2439 324 180 PflMI CCAn_nnn'nTGG Cuts at: 0 1687 2943 Size: 1687 1256 PfoI T'CCnGG_A Cuts at: 0 2896 2943 Size: 2896 47 PpuMI rG'GwC_Cy Cuts at: 0 420 2943 Size: 420 2523 PshAI GACnn'nnGTC Cuts at: 0 2771 2943 Size: 2771 172 PstI C_TGCA'G Cuts at: 0 2664 2943 Size: 2664 279 PvuII CAG'CTG Cuts at: 0 397 831 1296 1539 2793 2943 Size: 397 434 465 243 1254 150 Fragments arranged by size: 1254 465 434 397 243 150 SacI G_AGCT'C Cuts at: 0 1041 1325 2015 2943 Size: 1041 284 690 928 Fragments arranged by size: 1041 928 690 284 ScaI AGT'ACT Cuts at: 0 2441 2943 Size: 2441 502 SfcI C'TryA_G Cuts at: 0 560 1470 2239 2660 2783 2943 Size: 560 910 769 421 123 160 Fragments arranged by size: 910 769 560 421 160 123 SmlI C'TyrA_G Cuts at: 0 1651 1729 1950 2943 Size: 1651 78 221 993 Fragments arranged by size: 1651 993 221 78 SnaBI TAC'GTA Cuts at: 0 2152 2943 Size: 2152 791 SpeI A'CTAG_T Cuts at: 0 1089 2943 Size: 1089 1854 SspI AAT'ATT Cuts at: 0 1695 2943 Size: 1695 1248 StuI AGG'CCT Cuts at: 0 2399 2943 Size: 2399 544 StyI C'CwwG_G Cuts at: 0 141 2295 2943 Size: 141 2154 648 Fragments arranged by size: 2154 648 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 140 357 1773 2129 2434 2943 Size: 140 217 1416 356 305 509 Fragments arranged by size: 1416 509 356 305 217 140 TatI w'GTAC_w Cuts at: 0 2439 2759 2851 2943 Size: 2439 320 92 92 Fragments arranged by size: 2439 320 92 92 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 122 154 678 710 1606 1638 1734 1766 Size: 122 32 524 32 896 32 96 32 Cuts at: 1766 2699 2731 2943 Size: 933 32 212 Fragments arranged by size: 933 896 524 212 122 96 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2062 2943 Size: 2062 881 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 162 732 2342 2943 Size: 162 570 1610 601 Fragments arranged by size: 1610 601 570 162 XmnI GAAnn'nnTTC Cuts at: 0 841 2943 Size: 841 2102 Enzymes that do cut: AarI AccI Acc65I AcuI AhdI AlfI AlwNI ApoI BaeI BanI BanII BbsI BfrBI BglII BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BstBI BstEII BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII KpnI MmeI MslI MspA1I NsiI NspI PflMI PfoI PpuMI PshAI PstI PvuII SacI ScaI SfcI SmlI SnaBI SpeI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AflIII AgeI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmtI BplI BsaBI BsaHI BsiEI BsiWI BsmBI BsrBI BsrFI BssHII BssSI BstAPI BstXI BtgI BtgZI DraIII DrdI EaeI EagI EciI EcoRV FseI FspI FspAI HincII HpaI KasI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SphI SrfI SwaI XbaI XhoI XmaI ZraI