################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BlnI BsaHI BsiEI BsiWI BsmI BsoBI BspDI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 1654 GTAGAC GT^[AC][GT]AC AccIII 48 TCCGGA T^CCGGA AflII 403 CTTAAG C^TTAAG ApaI 2060 GGGCCC GGGCC^C Asp700I 1937 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 202 GAGCAC G[AT]GC[AT]^C BanI 413 GGTGCC G^G[CT][GA]CC BanII 2060 GGGCCC G[GA]GC[CT]^C BbsI 1941 GTCTTC GAAGAC(2/6) BbvCI 242 CCTCAGC CCTCAGC(-5/-2) BclI 570 TGATCA T^GATCA BfrI 403 CTTAAG C^TTAAG BglI 1042 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1055 AGATCT A^GATCT BlpI 1281 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1281 GCTGAGC GC^T[ACGT]AGC BpuAI 1941 GTCTTC GAAGAC(2/6) BsaAI 1751 CACGTA [CT]AC^GT[GA] BsaBI 565 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 48 TCCGGA [AT]^CCGG[AT] BseAI 48 TCCGGA T^CCGGA BsiHKAI 202 GAGCAC G[AT]GC[AT]^C BsmBI 1192 GAGACG CGTCTC(1/5) Bsp1407I 612 TGTACA T^GTACA BspEI 48 TCCGGA T^CCGGA BsrDI 606 CATTGC GCAATG(2/0) BsrGI 612 TGTACA T^GTACA CelII 1281 GCTGAGC GC^T[ACGT]AGC CfoI 250 GCGC GCG^C DraII 725 AGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 253 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 1597 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 725 AGGTCCT [GA]G^G[ACGT]CC[CT] FbaI 570 TGATCA T^GATCA HgaI 7 GCGTC GACGC(5/10) HhaI 250 GCGC GCG^C HinP1I 250 GCGC G^CGC HincII 1146 GTCAAC GT[CT]^[GA]AC HindII 1146 GTCAAC GT[CT]^[GA]AC MaeII 1752 ACGT A^CGT MamI 565 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 48 TCCGGA T^CCGGA MslI 1419 CATGGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 632 CAGCTG C[AC]G^C[GT]G PflMI 529 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 725 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 632 CAGCTG CAG^CTG SapI 581 GCTCTTC GCTCTTC(1/4) ScaI 1837 AGTACT AGT^ACT SexAI 254 ACCAGGT A^CC[AT]GGT SfcI 518 CTGTAG C^T[GA][CT]AG SspBI 612 TGTACA T^GTACA StuI 1767 AGGCCT AGG^CCT Van91I 529 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1364 CCAGTCCCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1937 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcsI 672 GAATTT [GA]^AATT[CT] 2053 AAATTC AlwNI 5 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG 913 CAGGATCTG ApoI 672 GAATTT [GA]^AATT[CT] 2053 AAATTC AvaII 726 GGTCC G^G[AT]CC 1564 GGTCC BalI 29 TGGCCA TGG^CCA 335 TGGCCA BmyI 202 GAGCAC G[AGT]GC[ACT]^C 2060 GGGCCC BseRI 1379 CTCCTC GAGGAG(10/8) 352 GAGGAG Bsp1286I 202 GAGCAC G[AGT]GC[ACT]^C 2060 GGGCCC BspMI 232 GCAGGT ACCTGC(4/8) 1404 GCAGGT BsrBI 1443 CCGCTC CCGCTC(-3/-3) 1822 GAGCGG EaeI 29 TGGCCA [CT]^GGCC[GA] 335 TGGCCA MluNI 29 TGGCCA TGG^CCA 335 TGGCCA MscI 29 TGGCCA TGG^CCA 335 TGGCCA NspI 1571 ACATGC [GA]CATG^[CT] 1947 ACATGC PshAI 82 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2080 GACACTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BcnI BfaI BpmI BsaI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII