(Circular) (Six-base) MAPSORT of: pFN21AE4194.seq Check: 6718 from: 1 to: 6814 pFN21AE4194 6814 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2228 2228 Size: 6814 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4624 278 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3660 4157 1937 Size: 1723 497 4594 Fragments arranged by size: 4594 1723 497 Acc65I G'GTAC_C Cuts at: 4135 4135 Size: 6814 AclI AA'CG_TT Cuts at: 1563 4941 5314 1563 Size: 3378 373 3063 Fragments arranged by size: 3378 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2475 2615 2771 3338 3722 4877 6261 6529 Size: 843 140 156 567 384 1155 1384 268 Cuts at: 6529 1632 Size: 1917 Fragments arranged by size: 1917 1384 1155 843 567 384 268 156 140 AflII C'TTAA_G Cuts at: 820 1017 2408 820 Size: 197 1391 5226 Fragments arranged by size: 5226 1391 197 AflIII A'CryG_T Cuts at: 1203 5713 6690 1203 Size: 4510 977 1327 Fragments arranged by size: 4510 1327 977 AgeI A'CCGG_T Cuts at: 5645 5645 Size: 6814 AhdI GACnn_n'nnGTC Cuts at: 5543 5543 Size: 6814 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6814 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6814 AlwNI CAG_nnn'CTG Cuts at: 2015 2923 4557 6129 2015 Size: 908 1634 1572 2700 Fragments arranged by size: 2700 1634 1572 908 ApaI G_GGCC'C Cuts at: 4069 4069 Size: 6814 ApaLI G'TGCA_C Cuts at: 4871 6027 4871 Size: 1156 5658 ApoI r'AATT_y Cuts at: 1493 2677 4058 4123 4349 4501 6610 1493 Size: 1184 1381 65 226 152 2109 1697 Fragments arranged by size: 2109 1697 1381 1184 226 152 65 AseI AT'TA_AT Cuts at: 160 1032 5368 160 Size: 872 4336 1606 Fragments arranged by size: 4336 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 AvaI C'yCGr_G Cuts at: 1870 1943 4139 1870 Size: 73 2196 4545 Fragments arranged by size: 4545 2196 73 BamHI G'GATC_C Cuts at: 4144 4511 4144 Size: 367 6447 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2418 4135 5590 618 Size: 325 217 627 631 1717 1455 1842 Fragments arranged by size: 1842 1717 1455 631 627 325 217 BanII G_rGCy'C Cuts at: 1391 4069 4133 1391 Size: 2678 64 4072 Fragments arranged by size: 4072 2678 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3939 928 Size: 971 2040 3803 Fragments arranged by size: 3803 2040 971 BbvCI CC'TCA_GC Cuts at: 2248 2248 Size: 6814 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5005 5039 1903 Size: 34 3068 34 3678 Fragments arranged by size: 3678 3068 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4706 5916 4706 Size: 1210 5604 BclI T'GATC_A Cuts at: 1548 2575 1548 Size: 1027 5787 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3053 5425 136 Size: 107 122 71 631 605 1381 2372 1525 Fragments arranged by size: 2372 1525 1381 631 605 122 107 71 BglII A'GATC_T Cuts at: 3060 6809 3060 Size: 3749 3065 BlpI GC'TnA_GC Cuts at: 3287 4231 3287 Size: 944 5870 Bme1580I G_kGCm'C Cuts at: 1790 4069 4875 6031 1790 Size: 2279 806 1156 2573 Fragments arranged by size: 2573 2279 1156 806 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6814 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2040 3363 3429 Size: 416 53 83 399 24 623 1323 66 Cuts at: 3429 3999 4545 5503 442 Size: 570 546 958 1753 Fragments arranged by size: 1753 1323 958 623 570 546 416 399 83 66 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6814 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2425 2517 3188 3689 3701 3942 5474 1239 Size: 1186 92 671 501 12 241 1532 2579 Fragments arranged by size: 2579 1532 1186 671 501 241 92 12 Bpu10I CC'TnA_GC Cuts at: 2248 2248 Size: 6814 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2978 3131 4939 5804 6102 2978 Size: 153 1808 865 298 3690 Fragments arranged by size: 3690 1808 865 298 153 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2099 2322 2409 5477 882 Size: 617 600 223 87 3068 2219 Fragments arranged by size: 3068 2219 617 600 223 87 BsaAI yAC'GTr Cuts at: 493 1204 3758 4605 493 Size: 711 2554 847 2702 Fragments arranged by size: 2702 2554 847 711 BsaBI GATnn'nnATC Cuts at: 2574 6808 2574 Size: 4234 2580 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4621 5003 5638 275 Size: 53 83 186 936 3088 382 635 1451 Fragments arranged by size: 3088 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2053 5247 5645 5919 6066 6477 2053 Size: 3194 398 274 147 411 2390 Fragments arranged by size: 3194 2390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2544 2574 3307 3337 1178 Size: 30 165 30 1141 30 733 30 4655 Fragments arranged by size: 4655 1141 733 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2372 3375 1186 Size: 1186 1003 4625 Fragments arranged by size: 4625 1186 1003 BseYI C'CCAG_C Cuts at: 3486 3491 6017 6513 3486 Size: 5 2526 496 3787 Fragments arranged by size: 3787 2526 496 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2109 3459 3792 1163 Size: 573 373 1350 333 4185 Fragments arranged by size: 4185 1350 573 373 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4264 5025 6053 1540 Size: 275 186 2263 761 1028 2301 Fragments arranged by size: 2301 2263 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 4875 4960 6031 2211 Size: 2664 85 1071 2994 Fragments arranged by size: 2994 2664 1071 85 BsmI GAATG_Cn' Cuts at: 4325 4418 4325 Size: 93 6721 BsmBI CGTCTCn'nnnn_ Cuts at: 3191 3191 Size: 6814 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 4069 4133 4875 4960 6031 1391 Size: 399 421 1858 64 742 85 1071 2174 Fragments arranged by size: 2174 1858 1071 742 421 399 85 64 BspEI T'CCGG_A Cuts at: 2053 6477 2053 Size: 4424 2390 BspHI T'CATG_A Cuts at: 4703 6433 4703 Size: 1730 5084 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2228 3400 4169 844 Size: 1384 1172 769 3489 Fragments arranged by size: 3489 1384 1172 769 BsrBI CCG'CTC Cuts at: 1394 1823 3450 3829 4701 1394 Size: 429 1627 379 872 3507 Fragments arranged by size: 3507 1627 872 429 379 BsrDI GCAATG_nn' Cuts at: 65 1248 2610 5309 5483 65 Size: 1183 1362 2699 174 1396 Fragments arranged by size: 2699 1396 1362 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5458 5645 1700 Size: 110 3648 187 2869 Fragments arranged by size: 3648 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2617 96 Size: 2521 4293 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6814 BssSI C'ACGA_G Cuts at: 4874 5886 4874 Size: 1012 5802 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6814 BstBI TT'CG_AA Cuts at: 5618 5618 Size: 6814 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6814 BstYI r'GATC_y Cuts at: 1972 2920 3060 3442 3675 4144 4511 4896 4913 Size: 948 140 382 233 469 367 385 17 Cuts at: 4913 6365 6451 6463 6809 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 948 469 385 382 367 346 233 140 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3423 3523 3565 3765 3780 513 Size: 552 939 1419 100 42 200 15 3547 Fragments arranged by size: 3547 1419 939 552 200 100 42 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6075 Fragments arranged by size: 6075 683 56 BtsI GCAGTG_nn' Cuts at: 2317 4330 5124 5144 2317 Size: 2013 794 20 3987 Fragments arranged by size: 3987 2013 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6814 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6779 DraI TTT'AAA Cuts at: 4118 4118 Size: 6814 DraIII CAC_nnn'GTG Cuts at: 2263 2263 Size: 6814 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5821 809 Size: 507 4505 1802 Fragments arranged by size: 4505 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2340 4261 Size: 54 1425 50 275 18 204 306 1921 Cuts at: 4261 5150 6779 8 Size: 889 1629 43 Fragments arranged by size: 1921 1629 1425 889 306 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4261 1537 Size: 275 2449 4090 Fragments arranged by size: 4090 2449 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2451 2593 2796 2956 3027 4744 1353 Size: 1098 142 203 160 71 1717 3423 Fragments arranged by size: 3423 1717 1098 203 160 142 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5401 5775 5921 387 Size: 1064 138 3812 374 146 1280 Fragments arranged by size: 3812 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2425 2475 2517 2615 2771 3188 3338 Size: 393 793 50 42 98 156 417 150 Cuts at: 3338 3689 3701 3722 3942 4877 5474 6261 6529 Size: 351 12 21 220 935 597 787 268 Cuts at: 6529 1239 Size: 1524 Fragments arranged by size: 1524 935 793 787 597 417 393 351 268 220 156 150 98 50 42 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 3606 3606 Size: 6814 EcoO109I rG'GnC_Cy Cuts at: 2731 2731 Size: 6814 EcoRI G'AATT_C Cuts at: 4123 4501 4123 Size: 378 6436 FspI TGC'GCA Cuts at: 5320 5320 Size: 6814 HaeII r_GCGC'y Cuts at: 1678 5961 1678 Size: 4283 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2374 2406 3050 3082 3434 Size: 32 135 32 802 32 644 32 352 Cuts at: 3434 3466 5455 5487 5529 5561 1373 Size: 32 1989 32 42 32 2626 Fragments arranged by size: 2626 1989 802 644 352 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3153 4158 4404 669 Size: 988 281 1215 1005 246 3079 Fragments arranged by size: 3079 1215 1005 988 281 246 HindIII A'AGCT_T Cuts at: 748 2506 2597 2942 748 Size: 1758 91 345 4620 Fragments arranged by size: 4620 1758 345 91 HpaI GTT'AAC Cuts at: 4404 4404 Size: 6814 KpnI G_GTAC'C Cuts at: 4139 4139 Size: 6814 MfeI C'AATT_G Cuts at: 4413 4413 Size: 6814 MluI A'CGCG_T Cuts at: 6690 6690 Size: 6814 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2542 4127 5928 6112 1305 Size: 1237 1585 1801 184 2007 Fragments arranged by size: 2007 1801 1585 1237 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 2342 10 Size: 54 1425 343 204 306 4482 Fragments arranged by size: 4482 1425 343 306 204 54 MslI CAynn'nnrTG Cuts at: 518 1166 3428 4772 5131 5290 518 Size: 648 2262 1344 359 159 2042 Fragments arranged by size: 2262 2042 1344 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2639 4230 4907 6055 6300 1606 Size: 1033 1591 677 1148 245 2120 Fragments arranged by size: 2120 1591 1148 1033 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3423 3523 3765 3780 513 Size: 552 939 1419 100 242 15 3547 Fragments arranged by size: 3547 1419 939 552 242 100 15 NdeI CA'TA_TG Cuts at: 387 387 Size: 6814 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6814 NotI GC'GGCC_GC Cuts at: 4261 4261 Size: 6814 NspI r_CATG'y Cuts at: 1132 1229 3580 3956 4172 5717 1132 Size: 97 2351 376 216 1545 2229 Fragments arranged by size: 2351 2229 1545 376 216 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6814 PciI A'CATG_T Cuts at: 5713 5713 Size: 6814 PflMI CCAn_nnn'nTGG Cuts at: 1108 2540 1108 Size: 1432 5382 PfoI T'CCnGG_A Cuts at: 2441 2733 3678 3937 2441 Size: 292 945 259 5318 Fragments arranged by size: 5318 945 292 259 PmeI GTTT'AAAC Cuts at: 4118 4118 Size: 6814 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4883 4915 6396 6428 4883 Size: 32 1481 32 5269 Fragments arranged by size: 5269 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2731 2731 Size: 6814 PshAI GACnn'nnGTC Cuts at: 1613 2091 4089 1613 Size: 478 1998 4338 Fragments arranged by size: 4338 1998 478 PsiI TTA'TAA Cuts at: 4384 4384 Size: 6814 PspOMI G'GGCC_C Cuts at: 4065 4065 Size: 6814 PstI C_TGCA'G Cuts at: 830 4166 830 Size: 3336 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6814 PvuII CAG'CTG Cuts at: 2639 2639 Size: 6814 SalI G'TCGA_C Cuts at: 1936 4156 1936 Size: 2220 4594 SapI GCTCTTCn'nnn_ Cuts at: 2593 2593 Size: 6814 SbfI CC_TGCA'GG Cuts at: 4166 4166 Size: 6814 ScaI AGT'ACT Cuts at: 3844 5062 6774 3844 Size: 1218 1712 3884 Fragments arranged by size: 3884 1712 1218 SexAI A'CCwGG_T Cuts at: 2259 2259 Size: 6814 SfcI C'TryA_G Cuts at: 826 1045 2523 4162 5297 5978 6169 826 Size: 219 1478 1639 1135 681 191 1471 Fragments arranged by size: 1639 1478 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6814 SmaI CCC'GGG Cuts at: 1872 4141 1872 Size: 2269 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 2993 3146 4918 5819 6081 Size: 197 926 465 585 153 1772 901 262 Cuts at: 6081 820 Size: 1553 Fragments arranged by size: 1772 1553 926 901 585 465 262 197 153 SnaBI TAC'GTA Cuts at: 493 4605 493 Size: 4112 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6814 SphI G_CATG'C Cuts at: 1132 4172 1132 Size: 3040 3774 SspI AAT'ATT Cuts at: 5 52 4738 5 Size: 47 4686 2081 Fragments arranged by size: 4686 2081 47 StuI AGG'CCT Cuts at: 1517 3774 1517 Size: 2257 4557 StyI C'CwwG_G Cuts at: 513 1065 2004 2178 3423 3523 3765 3780 4253 Size: 552 939 174 1245 100 242 15 473 Cuts at: 4253 513 Size: 3074 Fragments arranged by size: 3074 1245 939 552 473 242 174 100 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2391 4841 4858 5011 5196 5641 325 Size: 1255 811 2450 17 153 185 445 1498 Fragments arranged by size: 2450 1498 1255 811 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2617 3842 5060 Size: 274 80 33 51 1443 640 1225 1218 Cuts at: 5060 6772 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1225 1218 640 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2544 2576 3307 3339 6774 6806 2544 Size: 32 731 32 3435 32 2552 Fragments arranged by size: 3435 2552 731 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6814 XbaI T'CTAG_A Cuts at: 4150 4150 Size: 6814 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3376 3376 Size: 6814 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6814 XmaI C'CCGG_G Cuts at: 1870 4139 1870 Size: 2269 4545 XmnI GAAnn'nnTTC Cuts at: 3946 4943 3946 Size: 997 5817 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4622 276 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI