(Linear) (Six-base) MAPSORT of: KIEE4194.seq Check: 1070 from: 1 to: 2109 KIEE4194.orf With 182 enzymes: SgfI * July 3, 2012 18:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 223 2109 Size: 223 1886 AccI GT'mk_AC Cuts at: 0 1655 2109 Size: 1655 454 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 470 610 766 1333 1717 2109 Size: 470 140 156 567 384 392 Fragments arranged by size: 567 470 392 384 156 140 AflII C'TTAA_G Cuts at: 0 403 2109 Size: 403 1706 AlwNI CAG_nnn'CTG Cuts at: 0 10 918 2109 Size: 10 908 1191 Fragments arranged by size: 1191 908 10 ApaI G_GGCC'C Cuts at: 0 2064 2109 Size: 2064 45 ApoI r'AATT_y Cuts at: 0 672 2053 2109 Size: 672 1381 56 Fragments arranged by size: 1381 672 56 BanI G'GyrC_C Cuts at: 0 413 2109 Size: 413 1696 BanII G_rGCy'C Cuts at: 0 2064 2109 Size: 2064 45 BbsI GAAGACnn'nnnn_ Cuts at: 0 1934 2109 Size: 1934 175 BbvCI CC'TCA_GC Cuts at: 0 243 2109 Size: 243 1866 BclI T'GATC_A Cuts at: 0 570 2109 Size: 570 1539 BglI GCCn_nnn'nGGC Cuts at: 0 1048 2109 Size: 1048 1061 BglII A'GATC_T Cuts at: 0 1055 2109 Size: 1055 1054 BlpI GC'TnA_GC Cuts at: 0 1282 2109 Size: 1282 827 Bme1580I G_kGCm'C Cuts at: 0 2064 2109 Size: 2064 45 BmrI ACTGGGnnnn_n' Cuts at: 0 35 1358 1424 1994 2109 Size: 35 1323 66 570 115 Fragments arranged by size: 1323 570 115 66 35 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 420 512 1183 1684 1696 1937 2109 Size: 420 92 671 501 12 241 172 Fragments arranged by size: 671 501 420 241 172 92 12 Bpu10I CC'TnA_GC Cuts at: 0 243 2109 Size: 243 1866 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 973 1126 2109 Size: 973 153 983 Fragments arranged by size: 983 973 153 BsaI GGTCTCn'nnnn_ Cuts at: 0 94 317 404 2109 Size: 94 223 87 1705 Fragments arranged by size: 1705 223 94 87 BsaAI yAC'GTr Cuts at: 0 1753 2109 Size: 1753 356 BsaBI GATnn'nnATC Cuts at: 0 569 2109 Size: 569 1540 BsaWI w'CCGG_w Cuts at: 0 48 2109 Size: 48 2061 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 539 569 1302 1332 2109 Size: 539 30 733 30 777 Fragments arranged by size: 777 733 539 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 367 1370 2109 Size: 367 1003 739 Fragments arranged by size: 1003 739 367 BseYI C'CCAG_C Cuts at: 0 1481 1486 2109 Size: 1481 5 623 Fragments arranged by size: 1481 623 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 1454 1787 2109 Size: 104 1350 333 322 Fragments arranged by size: 1350 333 322 104 BsiHKAI G_wGCw'C Cuts at: 0 206 2109 Size: 206 1903 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1186 2109 Size: 1186 923 Bsp1286I G_dGCh'C Cuts at: 0 206 2064 2109 Size: 206 1858 45 Fragments arranged by size: 1858 206 45 BspEI T'CCGG_A Cuts at: 0 48 2109 Size: 48 2061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 223 1395 2109 Size: 223 1172 714 Fragments arranged by size: 1172 714 223 BsrBI CCG'CTC Cuts at: 0 1445 1824 2109 Size: 1445 379 285 Fragments arranged by size: 1445 379 285 BsrDI GCAATG_nn' Cuts at: 0 605 2109 Size: 605 1504 BsrGI T'GTAC_A Cuts at: 0 612 2109 Size: 612 1497 BstYI r'GATC_y Cuts at: 0 915 1055 1437 1670 2109 Size: 915 140 382 233 439 Fragments arranged by size: 915 439 382 233 140 BtgI C'CryG_G Cuts at: 0 1418 1518 1560 1760 1775 2109 Size: 1418 100 42 200 15 334 Fragments arranged by size: 1418 334 200 100 42 15 BtsI GCAGTG_nn' Cuts at: 0 312 2109 Size: 312 1797 DraIII CAC_nnn'GTG Cuts at: 0 258 2109 Size: 258 1851 EaeI y'GGCC_r Cuts at: 0 29 335 2109 Size: 29 306 1774 Fragments arranged by size: 1774 306 29 EarI CTCTTCn'nnn_ Cuts at: 0 446 588 791 951 1022 2109 Size: 446 142 203 160 71 1087 Fragments arranged by size: 1087 446 203 160 142 71 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 420 470 512 610 766 1183 1333 1684 Size: 420 50 42 98 156 417 150 351 Cuts at: 1684 1696 1717 1937 2109 Size: 12 21 220 172 Fragments arranged by size: 420 417 351 220 172 156 150 98 50 42 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 1601 2109 Size: 1601 508 EcoO109I rG'GnC_Cy Cuts at: 0 726 2109 Size: 726 1383 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 369 401 1045 1077 1429 1461 2109 Size: 369 32 644 32 352 32 648 Fragments arranged by size: 648 644 369 352 32 32 32 HincII GTy'rAC Cuts at: 0 1148 2109 Size: 1148 961 HindIII A'AGCT_T Cuts at: 0 501 592 937 2109 Size: 501 91 345 1172 Fragments arranged by size: 1172 501 345 91 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 537 2109 Size: 537 1572 MscI TGG'CCA Cuts at: 0 31 337 2109 Size: 31 306 1772 Fragments arranged by size: 1772 306 31 MslI CAynn'nnrTG Cuts at: 0 1423 2109 Size: 1423 686 MspA1I CmG'CkG Cuts at: 0 634 2109 Size: 634 1475 NcoI C'CATG_G Cuts at: 0 1418 1518 1760 1775 2109 Size: 1418 100 242 15 334 Fragments arranged by size: 1418 334 242 100 15 NspI r_CATG'y Cuts at: 0 1575 1951 2109 Size: 1575 376 158 Fragments arranged by size: 1575 376 158 PflMI CCAn_nnn'nTGG Cuts at: 0 535 2109 Size: 535 1574 PfoI T'CCnGG_A Cuts at: 0 436 728 1673 1932 2109 Size: 436 292 945 259 177 Fragments arranged by size: 945 436 292 259 177 PpuMI rG'GwC_Cy Cuts at: 0 726 2109 Size: 726 1383 PshAI GACnn'nnGTC Cuts at: 0 86 2084 2109 Size: 86 1998 25 Fragments arranged by size: 1998 86 25 PspOMI G'GGCC_C Cuts at: 0 2060 2109 Size: 2060 49 PvuII CAG'CTG Cuts at: 0 634 2109 Size: 634 1475 SapI GCTCTTCn'nnn_ Cuts at: 0 588 2109 Size: 588 1521 ScaI AGT'ACT Cuts at: 0 1839 2109 Size: 1839 270 SexAI A'CCwGG_T Cuts at: 0 254 2109 Size: 254 1855 SfcI C'TryA_G Cuts at: 0 518 2109 Size: 518 1591 SmlI C'TyrA_G Cuts at: 0 403 988 1141 2109 Size: 403 585 153 968 Fragments arranged by size: 968 585 403 153 StuI AGG'CCT Cuts at: 0 1769 2109 Size: 1769 340 StyI C'CwwG_G Cuts at: 0 173 1418 1518 1760 1775 2109 Size: 173 1245 100 242 15 334 Fragments arranged by size: 1245 334 242 173 100 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 386 2109 Size: 386 1723 TatI w'GTAC_w Cuts at: 0 612 1837 2109 Size: 612 1225 272 Fragments arranged by size: 1225 612 272 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 539 571 1302 1334 2109 Size: 539 32 731 32 775 Fragments arranged by size: 775 731 539 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1371 2109 Size: 1371 738 XmnI GAAnn'nnTTC Cuts at: 0 1941 2109 Size: 1941 168 Enzymes that do cut: AarI AccI AcuI AflII AlwNI ApaI ApoI BanI BanII BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI BstYI BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NspI PflMI PfoI PpuMI PshAI PspOMI PvuII SapI ScaI SexAI SfcI SmlI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BmgBI BmtI BplI BsaHI BsiEI BsiWI BsmI BspHI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI