################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccIII AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I MamI MfeI MluI MroI MseI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI PvuI RcaI RsrII SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 707 GTCGAC GT^[AC][GT]AC AclI 902 AACGTT AA^CGTT AlwNI 889 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 71 TGGCCA TGG^CCA BbsI 948 GAAGAC GAAGAC(2/6) BfaI 997 CTAG C^TAG BglI 34 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 948 GAAGAC GAAGAC(2/6) BseRI 856 GAGGAG GAGGAG(10/8) BsgI 28 CTGCAC GTGCAG(16/14) BsiWI 88 CGTACG C^GTACG BsrBI 388 GAGCGG CCGCTC(-3/-3) BstXI 293 CCAGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 258 GGATCT [GA]^GATC[CT] DsaI 544 CCGCGG C^C[GA][CT]GG EcoT14I 440 CCTTGG C^C[AT][AT]GG EcoT22I 878 ATGCAT ATGCA^T HaeII 491 AGCGCC [GA]GCGC^[CT] HapII 279 CCGG C^CGG HpaII 279 CCGG C^CGG KspI 544 CCGCGG CCGC^GG MaeI 997 CTAG C^TAG MflI 258 GGATCT [GA]^GATC[CT] MluNI 71 TGGCCA TGG^CCA MscI 71 TGGCCA TGG^CCA MspI 279 CCGG C^CGG NsiI 878 ATGCAT ATGCA^T Psp1406I 902 AACGTT AA^CGTT PstI 283 CTGCAG CTGCA^G SacII 544 CCGCGG CCGC^GG SalI 707 GTCGAC G^TCGAC SphI 965 GCATGC GCATG^C StuI 469 AGGCCT AGG^CCT StyI 440 CCTTGG C^C[AT][AT]GG TaqI 708 TCGA T^CGA TthHB8I 708 TCGA T^CGA XhoII 258 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 258 GGATC GGATC(4/5) 791 GGATC BanI 701 GGCACC G^G[CT][GA]CC 1034 GGTGCC BsrI 215 ACTGG ACTGG(1/-1) 943 CCAGT BstEII 429 GGTCACC G^GT[ACGT]ACC 462 GGTGACC BstPI 429 GGTCACC G^GT[ACGT]ACC 462 GGTGACC CfoI 79 GCGC GCG^C 492 GCGC EaeI 71 TGGCCA [CT]^GGCC[GA] 541 TGGCCG EcoNI 45 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 597 CCTCTCGCAGG EcoO65I 429 GGTCACC G^GT[ACGT]ACC 462 GGTGACC HhaI 79 GCGC GCG^C 492 GCGC HinP1I 79 GCGC G^CGC 492 GCGC HincII 707 GTCGAC GT[CT]^[GA]AC 731 GTCAAC HindII 707 GTCGAC GT[CT]^[GA]AC 731 GTCAAC HinfI 891 GAGTC G^A[ACGT]TC 920 GAGTC MaeII 220 ACGT A^CGT 903 ACGT MslI 567 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1014 CATGATCGTG NspI 128 ACATGC [GA]CATG^[CT] 965 GCATGC PleI 891 GAGTC GAGTC(4/5) 920 GAGTC PshAI 674 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 724 GACAGAAGTC SacI 274 GAGCTC GAGCT^C 651 GAGCTC SfcI 181 CTACAG C^T[GA][CT]AG 283 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AfaI AluI ApaI AspHI AvaII BanII BbvI BlpI BmyI BpmI Bpu1102I BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CelII Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HphI ItaI MaeIII MboI MboII MnlI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI TseI Tsp45I TspRI